Review
Health Care Sciences & Services
Gina Gheorghe, Nicolae Bacalbasa, Gabriela Ceobanu, Madalina Ilie, Valentin Enache, Gabriel Constantinescu, Simona Bungau, Camelia Cristina Diaconu
Summary: GISTs are the most common mesenchymal neoplasms of the gastrointestinal tract, originating from interstitial cells of Cajal. They are often found in the stomach and small intestine, characterized by gene mutations, and treated with surgery, endoscopic treatment, and chemotherapy.
JOURNAL OF PERSONALIZED MEDICINE
(2021)
Article
Oncology
Daniele Fanale, Lorena Incorvaia, Giuseppe Badalamenti, Ida De Luca, Laura Algeri, Annalisa Bonasera, Lidia Rita Corsini, Chiara Brando, Antonio Russo, Juan Lucio Iovanna, Viviana Bazan
Summary: This study aimed to investigate the correlation between circulating levels of soluble immune checkpoints and survival in metastatic GIST patients. It found that high baseline levels of sPD-1, sPD-L1, sBTN3A1, and pan-BTN3As were associated with shorter progression-free survival (PFS) and poor prognosis, while lower levels were linked to longer PFS. Additional multivariate analysis highlighted the importance of specific plasma concentrations of sPD-L1 and pan-sBTN3As, as well as the absence of certain KIT exon 11 mutations, in predicting longer PFS in mGIST patients.
Article
Oncology
Xiaojing Lu, Yuzhi Pang, Hui Cao, Xiaoxiao Liu, Lin Tu, Yanying Shen, Xiaona Jia, Jen-Chieh Lee, Yuexiang Wang
Summary: CDK1 is highly expressed in advanced GIST and essential for GIST proliferation. Inhibition of CDK1 with RO-3306 showed promising results in reducing tumor growth in both imatinib-resistant and imatinib-sensitive GIST xenograft mouse models, suggesting CDK1 as a potential therapeutic target for advanced GIST patients.
Review
Oncology
Chiao-En Wu, Chiao-Ping Chen, Wen-Kuan Huang, Yi-Ru Pan, Erhan Aptullahoglu, Chun-Nan Yeh, John Lunec
Summary: This article reviews the role of p53 in GIST and proposes targeting the p53 pathway as a novel treatment strategy for GIST.
FRONTIERS IN ONCOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Jiatong Xu, Jiaying Li, Yan Li, Xiaoxiao Shi, Huadong Zhu, Limeng Chen
Summary: This study utilizes a mouse model and proteomics and metabolomics analyses to uncover the molecular mechanisms and potential pathways of sepsis-associated acute kidney injury.
Article
Oncology
Xin Fan, He Han, Zhiyu Sun, Liwen Zhang, Gong Chen, Said Abdulrahman Salim Mzee, Hanqing Yang, Jixiang Chen
Summary: Patients with gastrointestinal stromal tumors who experience gastrointestinal bleeding have a significantly worse overall survival compared to those without bleeding, while recurrence-free survival is not significantly affected. Factors such as age, location of the tumor in the small intestine, tumor diameter, mitotic index, and tumor rupture may increase the risk of gastrointestinal bleeding in GIST patients.
TECHNOLOGY IN CANCER RESEARCH & TREATMENT
(2021)
Article
Veterinary Sciences
Hui Zhang, Guyue Hu, Doukun Lu, Gang Zhao, Yiqiu Zhang, Muhammad Zubair, Yingyu Chen, Changmin Hu, Xi Chen, Jianguo Chen, Huanchun Chen, Liguo Yang, Aizhen Guo
Summary: This study compared the secretomes of virulent and attenuated strains of M.bovis to identify potential pathogenesis-related secreted proteins. Results showed 34 proteins were more abundant and uniquely expressed in the virulent strain, potentially associated with the virulence of M.bovis.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Oncology
Lorena Incorvaia, Dario De Biase, Margherita Nannini, Elena Fumagalli, Bruno Vincenzi, Ida De Luca, Chiara Brando, Alessandro Perez, Maria A. Pantaleo, Silvia Gasperoni, Lorenzo D'Ambrosio, Giovanni Grignani, Thais Maloberti, Erika Pedone, Tancredi Didier Bazan Russo, Alessandro Mazzocca, Laura Algeri, Alessandra Dimino, Nadia Barraco, Roberta Serino, Valerio Gristina, Antonio Galvano, Viviana Bazan, Antonio Russo, Giuseppe Badalamenti
Summary: This study investigated the prognostic role of KIT or PDGFRA-VAF in GIST patients and found that higher VAF levels were independent predictors of poor prognosis and survival in localized GIST patients with KIT or PDGFRA mutations. This study is important for the prognostication and choice of adjuvant treatment in patients with gastrointestinal stromal tumors.
Review
Medicine, General & Internal
Zhenjie Li, Dengming Zhang, Chunlin Mo, Peijin Zhu, Xiaoxi Fan, Tingyong Tang
Summary: This study aimed to verify the prognostic role of the prognostic nutritional index (PNI) in patients with gastrointestinal stromal tumors (GISTs). The analysis of 8 eligible studies showed that an elevated PNI was associated with a better recurrence-free survival. The findings suggest that PNI may be a useful indicator for assessing the risk of recurrence in Chinese patients with GISTs.
Article
Oncology
Xiaofan Mao, Xuezhu Yang, Xiangping Chen, Sifei Yu, Si Yu, Beiying Zhang, Yong Ji, Yihao Chen, Ying Ouyang, Wei Luo
Summary: This study analyzed multiple cell types in GIST, including tumor cells, T cells, macrophages, etc., and revealed their interactions as well as their clinical relevance and prognostic values. The research uncovered the heterogeneity of the GIST microenvironment, providing a novel theoretical basis for the treatment of GIST.
Article
Immunology
Dean Rao, Chengpeng Yu, Tiantian Wang, Jiaqi Sheng, Enjun Lv, Huifang Liang, Wenjie Huang, Hanhua Dong
Summary: IL4I1 is a secreted enzyme involved in the regulation of adaptive immune responses and affects tumor development. IL4I1 is associated with the infiltration status of immune cells and may serve as a potential biomarker and therapeutic target.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Review
Immunology
Matthieu Roulleaux Dugage, Robin Lewis Jones, Jonathan Trent, Stephane Champiat, Sarah Dumont
Summary: Gastrointestinal stromal tumors (GISTs) are a subtype of soft tissue sarcoma that are highly sensitive to imatinib and have immune infiltrated tumors with predominance of M2 macrophages, providing prognostic factors for response to ICI. While having immunosuppressive attributes, there is also interest in exploring immunotherapeutic agents in GIST and highlighting the rationale for combining c-kit inhibition with immune checkpoint inhibitors.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Endocrinology & Metabolism
Siqi Wen, Jiajia Li, Jingru Yang, Biao Li, Na Li, Xianquan Zhan
Summary: This study identified significant down-acetylation of numerous proteins in NF-PitNETs, impacting various cellular processes and signaling pathways. Additionally, a direct competition relationship between acetylation and ubiquitination was observed in NF-PitNETs, shedding light on potential mechanisms for targeted therapy.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Surgery
Sheng-Chieh Lin, Hung-Hsuan Yen, Po-Chu Lee, I-Rue Lai
Summary: This study demonstrates that laparoscopic surgery is safe and oncologically feasible for gastric GISTs between 5 and 8 cm. The clinical characteristics, peri-operative factors, and oncological outcomes were similar between groups.
SURGICAL ENDOSCOPY AND OTHER INTERVENTIONAL TECHNIQUES
(2023)
Article
Biochemical Research Methods
Bo Wang, Long Zhao, Zhidong Gao, Jianyuan Luo, Haoran Zhang, Lin Gan, Kewei Jiang, Shan Wang, Yingjiang Ye, Zhanlong Shen
Summary: This study identified a total of 2904 acetylation sites from 1319 proteins in GIST, with quantitative data obtained for 2548 sites from 1169 proteins. Differentially expressed lysine acetylation sites were assessed, showing that 42 acetylated sites from 38 proteins were upregulated in the H/M risk group, while 48 acetylated sites from 44 proteins were downregulated, including Ki67 K1063Ac and FCHSD2 K24Ac as the most affected acetylated proteins.
CLINICAL PROTEOMICS
(2021)
Article
Cell Biology
Rei Noguchi, Yuki Yoshimatsu, Takuya Ono, Akane Sei, Kaoru Hirabayashi, Iwao Ozawa, Kazutaka Kikuta, Tadashi Kondo
Summary: TGCT is a mesenchymal tumor arising from the synovium of tendon sheath and joints, with diverse clinical behaviors requiring further research. A novel TGCT cell line, NCC-TGCT1-C1, was successfully established in this study, showing strong responses to anticancer drugs in vitro, making it a valuable tool for studying TGCT development and the effects of anticancer agents.
Article
Cell Biology
Rei Noguchi, Yuki Yoshimatsu, Takuya Ono, Akane Sei, Kaoru Hirabayashi, Iwao Ozawa, Kazutaka Kikuta, Tadashi Kondo
Summary: A novel LMS cell line, NCC-LMS2-C1, has been established from a 90-year-old female patient with pleomorphic LMS, showing continuous growth, spheroid formation, and invasion capability. This cell line will be valuable for studying the mechanisms of LMS progression and conducting high-throughput screening for anti-cancer drug discovery.
Article
Dermatology
Nozomi Jimura, Kazuyasu Fujii, Zhiwei Qiao, Ryuto Tsuchiya, Yuki Yoshimatsu, Tadashi Kondo, Takuro Kanekura
Summary: The study identified Src pathway as a potential target in combination therapy for CTCL, with Ponatinib enhancing apoptosis induced by HDACis.
JOURNAL OF DERMATOLOGICAL SCIENCE
(2021)
Article
Cell Biology
Rei Noguchi, Yuki Yoshimatsu, Takuya Ono, Akane Sei, Kaoru Hirabayashi, Iwao Ozawa, Kazutaka Kikuta, Tadashi Kondo
Summary: Pleomorphic liposarcoma (PLPS) is a rare and aggressive subtype of liposarcoma with limited treatment options. A novel patient-derived cell line, NCC-PLPS1-C1, has been successfully established and characterized for basic research and drug screening, demonstrating significant responses to various anti-cancer drugs.
Article
Medicine, Research & Experimental
Toru Hirozane, Mari Masuda, Teppei Sugano, Tetsuya Sekita, Naoko Goto, Toru Aoyama, Takato Sakagami, Yuko Uno, Hideki Moriyama, Masaaki Sawa, Naofumi Asano, Masaya Nakamura, Morio Matsumoto, Robert Nakayama, Tadashi Kondo, Akira Kawai, Eisuke Kobayashi, Tesshi Yamada
Summary: The study demonstrated that TNIK could be a potential therapeutic target in osteosarcoma, as its inhibition suppressed cell proliferation, altered metabolic network direction, and converted cell phenotype. These findings offer a new direction for osteosarcoma treatment.
Article
Cell Biology
Rei Noguchi, Yuki Yoshimatsu, Takuya Ono, Akane Sei, Kaoru Hirabayashi, Iwao Ozawa, Kazutaka Kikuta, Tadashi Kondo
Summary: DDLPS is a highly aggressive subtype of liposarcoma with complex karyotype and genomic features, leading to poor prognosis. A novel DDLPS cell line NCC-DDLPS2-C1 showed varying sensitivity to chemotherapy drugs, especially in three-dimensional cell culture. This cell line may serve as a valuable resource for DDLPS studies.
Article
Oncology
Kaoru Yamawaki, Yutaro Mori, Hiroaki Sakai, Yusuke Kanda, Daisuke Shiokawa, Haruka Ueda, Tatsuya Ishiguro, Kosuke Yoshihara, Kazunori Nagasaka, Takashi Onda, Tomoyasu Kato, Tadashi Kondo, Takayuki Enomoto, Koji Okamoto
Summary: By analyzing patient-derived ovarian cancer spheroids, it was found that cisplatin resistance is correlated with elevated levels of G6PD and active glutathione. Additionally, the combination of a G6PD inhibitor with cisplatin in vitro effectively inhibited spheroid proliferation.
Article
Gastroenterology & Hepatology
Yasuyuki Takamizawa, Dai Shida, Tomoko Horie, Shunsuke Tsukamoto, Minoru Esaki, Kazuaki Shimada, Tadashi Kondo, Yukihide Kanemitsu
Summary: This study evaluated the prognostic value of primary tumor location in patients with colorectal liver metastases, and found that rectal cancer had worse relapse-free survival and overall survival compared with left-sided colon cancer.
DISEASES OF THE COLON & RECTUM
(2023)
Article
Cell Biology
Ryuto Tsuchiya, Yuki Yoshimatsu, Rei Noguchi, Yooksil Sin, Takuya Ono, Taro Akiyama, Jun Sugaya, Fumihiko Nakatani, Naoki Kojima, Akihiko Yoshida, Seiji Ohtori, Akira Kawai, Tadashi Kondo
Summary: Undifferentiated pleomorphic sarcoma (UPS) is an aggressive sarcoma with no identifiable line of differentiation, often caused by radiation exposure, refractory to conventional chemotherapy, and surgical treatment remains the most important modality. To study novel treatment methods for UPS, a new UPS cell line NCC-UPS3-C1 was established, which was found to be sensitive to the histone deacetylase inhibitor romidepsin.
Article
Cell Biology
Yooksil Sin, Yuki Yoshimatsu, Rei Noguchi, Ryuto Tsuchiya, Takuya Ono, Taro Akiyama, Fumihiko Nakatani, Jun Sugaya, Akihiko Yoshida, Akira Kawai, Tadashi Kondo
Summary: The establishment of the NCC-MPNST6-C1 cell line from surgically resected MPNST tissue provides a valuable resource for studying MPNSTs. The cells exhibit characteristics similar to the original tumors, including constant proliferation and invasion capability, making them suitable for drug screening despite not forming tumors in nude mice. This indicates potential for the development of effective therapies for MPNSTs.
Article
Cell Biology
Takuya Ono, Rei Noguchi, Yuki Yoshimatsu, Ryuto Tsuchiya, Yooksil Sin, Rumi Nakagawa, Kaoru Hirabayashi, Iwao Ozawa, Kazutaka Kikuta, Tadashi Kondo
Summary: Giant cell tumor of bone (GCTB) is a rare bone tumor with a pathogenic gene mutation, requiring complete surgical resection for treatment, but often resulting in recurrence. A new GCTB cell line, NCC-GCTB4-C1, was successfully established in this study, showing potential for drug discovery and treatment.
Article
Cell Biology
Ryuto Tsuchiya, Yuki Yoshimatsu, Rei Noguchi, Yooksil Sin, Takuya Ono, Taro Akiyama, Jun Sugaya, Eisuke Kobayashi, Naoki Kojima, Akihiko Yoshida, Seiji Ohtori, Akira Kawai, Tadashi Kondo
Summary: In this study, a new MFS cell line, NCC-MFS5-C1, was established for investigating the pathogenesis of MFS and developing new treatment approaches. Through drug screening analysis using NCC-MFS5-C1 cells and four other MFS cell lines, it was found that Bortezomib and romidepsin showed stronger anti-tumor effects than the standard drug doxorubicin.
Article
Cell Biology
Aoi Komatsu, Kotaro Matsumoto, Yuki Yoshimatsu, Yooksil Sin, Arisa Kubota, Tomoki Saito, Ayaka Mizumoto, Shinya Ohashi, Manabu Muto, Rei Noguchi, Tadashi Kondo, Fuyuhiko Tamanoi
Summary: The CAM assay shows promise as a model for CIC-DUX4 sarcoma, demonstrating tumor formation and retention of the fusion gene in CAM-derived organoids.
Article
Health Care Sciences & Services
Rei Noguchi, Yuki Yoshimatsu, Yooksil Sin, Takuya Ono, Ryuto Tsuchiya, Hiroshi Yoshida, Tohru Kiyono, Yutaka Yonemura, Tadashi Kondo
Summary: Pseudomyxoma peritonei (PMP) is a condition characterized by the accumulation of mucus in the peritoneal cavity due to a mucinous tumor. The standard treatment for PMP is complete cytoreductive surgery and hyperthermic intraperitoneal chemotherapy. However, due to high recurrence rates and the invasive nature of the treatment, 30-40% of patients are unable to receive standard therapy, highlighting the need for novel treatments. In this study, a new PMP cell line, NCC-PMP1-C1, was established from metastatic PMP tissue, providing a potential resource for basic and pre-clinical research and drug screening for metastatic PMP.
JOURNAL OF PERSONALIZED MEDICINE
(2022)
Article
Health Care Sciences & Services
Ryuto Tsuchiya, Yuki Yoshimatsu, Rei Noguchi, Yooksil Sin, Takuya Ono, Taro Akiyama, Takeshi Hirose, Shintaro Iwata, Akihiko Yoshida, Seiji Ohtori, Akira Kawai, Tadashi Kondo
Summary: DDLPS is a highly malignant sarcoma characterized by the co-amplification of MDM2 and CDK4, with poor response to conventional chemotherapy. This study established a novel DDLPS cell line, NCC-DDLPS4-C1, which can be used for basic research and drug screening, identifying romidepsin as a potential candidate drug.
JOURNAL OF PERSONALIZED MEDICINE
(2021)
