期刊
CLINICAL BIOCHEMISTRY
卷 45, 期 18, 页码 1689-1693出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.clinbiochem.2012.09.001
关键词
Saliva carbohydrate antigen 15-3; Surface plasmon resonance; Gold/zinc oxide; Biacore SPR
资金
- National Science Council of the Republic of China [NSC 95-2218-E-002-054-MY3]
Objective: To evaluate a novel surface plasmon resonance (SPR) system for the direct measurement of tumor marker carbohydrate antigen 15-3 (CA15-3) in human saliva. Design and methods: We measured the presence of the tumor marker carbohydrate antigen 15-3 (CA15-3) in human saliva using 2 different surface plasmon resonance (SPR) systems. To compare the sensitivity of an SPR biosensor based on thin-film Au/ZnO and the Biacore SPR system, we prepared CA15-3 samples in saliva and analyzed intensity responses to the samples at various concentrations of CA15-3. Results: The linear detection range of CA15-3 in human saliva with the SIR system based on thin-film Au/ZnO was 2.5-20 U/mL (the cut-off point in cancer patients is around 4 U/mL). The linear range with the Biacore SPR system was 40-300 U/mL Conclusions: These results show that thin-film Au/ZnO-based SPR systems have higher sensitivity and can be used for measuring the levels of CA15-3 in human saliva without concentrating the samples. (C) 2012 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.
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