Elevated transrenal DNA (cell-free urine DNA) in patients with urinary tract infection compared to healthy controls

Objective: To study whether levels of transrenal DNA (Tr-DNA) are influenced by the presence of urinary tract infections (UTI). Methods: Tr-DNA concentrations were measured in 124 donors and 55 patients with UTI. Two methods for DNA extraction were compared. Results: UTI patients showed higher concentrations than the donors (normal range: 0-147 GE/mL). QIAamp MiniElute Virus Spin Kit was more efficient than QIAamp DNA Blood Mini Kit. Conclusion: Increased concentrations of Tr-DNA urine DNA are shown in presence of UTI. (C) 2008 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.