Review
Immunology
Stefania Cuzzubbo, Sara Mangsbo, Divya Nagarajan, Kinana Habra, Alan Graham Pockley, Stephanie E. B. McArdle
Summary: The discovery of tumor antigens has led to the development of cancer vaccines, but many antigens lack immunogenicity. Adjuvants are added to vaccine formulations to trigger immune responses. Aging can lead to immunosenescence and chronic inflammation.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Haochi Zhang, Na Pan, Cheng Ma, Bohui Liu, Lei Xiu, He Tong, Shouxin Sheng, Yanchen Liang, Haotian Li, Fangfei Ma, Xuemei Bao, Wei Hu, Xiao Wang
Summary: This study developed a vaccine composed of Staphylococcus aureus antigen MntC and Lactobacillus casei exopolysaccharides, which provided high levels of protection against S. aureus infection by enhancing immune protection function and inducing cellular immune response. The vaccine induced superior humoral immunity, increased IL-17A and IFN-gamma production, and promoted robust Th 17/gamma delta T 17 primary and recall responses, showing promise for future clinical application against S. aureus-induced pulmonary and cutaneous infection.
Article
Biotechnology & Applied Microbiology
Ekaterina M. Ryabchevskaya, Ekaterina A. Evtushenko, Dmitry L. Granovskiy, Peter A. Ivanov, Joseph G. Atabekov, Olga A. Kondakova, Nikolai A. Nikitin, Olga Karpova
Summary: The potential of stabilizing rPA for anthrax vaccine was demonstrated through the simultaneous application of tobacco mosaic virus spherical particles (SPs) and site-specific mutagenesis. The results support the development of a recombinant vaccine with modified rPA and SPs.
HUMAN VACCINES & IMMUNOTHERAPEUTICS
(2021)
Article
Food Science & Technology
Weiming Ouyang, Tao Xie, Hui Fang, David M. Frucht
Summary: Anthrax toxin is a key virulence factor of Bacillus anthracis. Anti-PA antibodies have been developed for the treatment of anthrax infection, but current assays do not fully capture the pathological functions of anthrax toxin. This study reports the development of a cell-based gene reporter potency assay to measure the effectiveness of anti-PA antibodies.
Article
Biotechnology & Applied Microbiology
Sakshi Bajoria, Kawaljit Kaur, Ozan S. Kumru, Greta Van Slyke, Jennifer Doering, Hayley Novak, Sergio A. Rodriguez Aponte, Neil C. Dalvie, Christopher A. Naranjo, Ryan S. Johnston, Judith Maxwell Silverman, Harry Kleanthous, J. Christopher Love, Nicholas J. Mantis, Sangeeta B. Joshi, David B. Volkin
Summary: Low-cost, refrigerator-stable COVID-19 vaccines are important for global accessibility and vaccine coverage. This study found that aluminum-salt and CpG adjuvants decrease the stability of the RBD-J antigen, but enhance the immune response in mice, with the AH+CpG formulation being the most effective at inducing SARS-CoV-2 pseudovirus neutralizing antibodies.
HUMAN VACCINES & IMMUNOTHERAPEUTICS
(2022)
Article
Immunology
Li-Na Zhai, Yue Zhao, Xiao-Lin Song, Tong-Tong Qin, Zhi-Jun Zhang, Jia-Zhen Wang, Cheng-Yu Sui, Li-Li Zhang, Meng Lv, Ling-Fei Hu, Dong-Sheng Zhou, Tong-Yu Fang, Wen-Hui Yang, Yan-Chun Wang
Summary: A low protease activity Bacillus anthracis strain A16R-5.1 was prepared by deleting five extracellular protease activity-associated genes and its lef gene using the CRISPR-Cas9 genome editing system. An anthrax vaccine was formulated from the mutant strain, which contained a lethal toxin-free culture supernatant extract with 50% protective antigen. The vaccine induced strong immune responses and provided full protection against lethal aerosol challenges in mice. Compared to the recombinant protective antigen vaccine, this culture supernatant extract anthrax vaccine showed superior immunoprotection against pulmonary anthrax.
EMERGING MICROBES & INFECTIONS
(2023)
Article
Immunology
Ntungufhadzeni M. Rathogwa, Katherine A. Scott, Pamela Opperman, Jacques Theron, Francois F. Maree
Summary: This study compared the efficacy of two adjuvant formulations combined with a structurally stabilized SAT2 vaccine antigen and found that the Montanide ISA 206B-adjuvanted vaccine elicited a higher SAT2 neutralizing antibody response and three times higher systemic IFN-gamma responses compared to the Quil-A Saponin-adjuvanted vaccine group at 14- and 28-days post-vaccination. Serum antibodies from the immunized animals reacted similarly to both the parental vaccine virus and mutated viruses simulating antigenic drift in nature.
Article
Multidisciplinary Sciences
Srinivas Kammanadiminti, Jason Comer, Gabriel Meister, Trevor Carnelley, Derek Toth, Shantha Kodihalli
Summary: In order to meet the requirements of the Animal Rule, the efficacy of monotherapy with ANTHRASIL was evaluated in blinded studies using rabbit and nonhuman primate models. The studies demonstrated that ANTHRASIL significantly improved survival rates in both animals, forming the basis for its approval by the FDA under the Animal Rule.
Article
Biotechnology & Applied Microbiology
Sarah M. Valencia, Athina Zacharia, Alexander Marin, Rebecca L. Matthews, Chia-Kuei Wu, Breana Myers, Chelsea Sanders, Simone Difilippantonio, Reinhard Kirnbauer, Richard B. Roden, Ligia A. Pinto, Robert H. Shoemaker, Alexander K. Andrianov, Jason D. Marshall
Summary: Current HPV vaccines provide protection against common HPV types, but many cancer-causing HPV types lack coverage. Utilization of PCEP as an adjuvant for the RG1-VLP vaccine showed superior immunogenicity compared to Alhydrogel, suggesting potential for dose sparing and longer-lasting humoral responses.
HUMAN VACCINES & IMMUNOTHERAPEUTICS
(2021)
Article
Materials Science, Biomaterials
Yiming Zhang, Zhiwen Hu, Xinxin Li, Yinghao Ding, Zhenghao Zhang, Xiangyang Zhang, Wenting Zheng, Zhimou Yang
Summary: The amino acid sequence of the peptide determines the adjuvant potency of Gel-gffy. The position and number of aromatic amino acids play a crucial role in the immunostimulatory activity of Gel-gffy. These findings provide valuable insights into designing peptide hydrogels as vaccine adjuvants.
BIOMATERIALS SCIENCE
(2022)
Article
Immunology
Meghdad Dehnavi, Setareh Haghighat, Mohammad Hossein Yazdi, Mehdi Mahdavi
Summary: The protective efficacy and immunological properties of recombinant autolysin, formulated in Montanide ISA266 and Alum adjuvants with Glucomannan as a polysaccharide, were assessed. Results showed that using Alum and Montanide ISA266 adjuvants with Glucomannan significantly increased total IgG and isotypes (IgG1 and IgG2a), enhanced cytokine secretion, and improved protectivity.
MICROBIAL PATHOGENESIS
(2023)
Article
Fisheries
Fei Sun, Jie Zhou, Yuanxing Zhang, Qin Liu, Qiyao Wang, Xiaohong Liu
Summary: An inactivated vaccine containing a compound adjuvant of ginseng stem leaf saponins and aluminum hydroxide gel (GSLS/Alum) was developed against Aeromonas salmonicida in turbot farming. The vaccine demonstrated a significant increase in serum antibody titer and a high relative percentage survival (RPS) of turbot. The formulation of GSLS and Alum proved to be a potential compound adjuvant in turbot vaccine development, showing excellent protection against natural infection.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Immunology
Maria Pino, Talha Abid, Susan Pereira Ribeiro, Venkata Viswanadh Edara, Katharine Floyd, Justin C. Smith, Muhammad Bilal Latif, Gabriela Pacheco-Sanchez, Debashis Dutta, Shelly Wang, Sanjeev Gumber, Shannon Kirejczyk, Joyce Cohen, Rachelle L. Stammen, Sherrie M. Jean, Jennifer S. Wood, Fawn Connor-Stroud, Jeroen Pollet, Wen-Hsiang Chen, Junfei Wei, Bin Zhan, Jungsoon Lee, Zhuyun Liu, Ulrich Strych, Neeta Shenvi, Kirk Easley, Daniela Weiskopf, Alessandro Sette, Justin Pollara, Dieter Mielke, Hongmei Gao, Nathan Eisel, Celia C. LaBranche, Xiaoying Shen, Guido Ferrari, Georgia D. Tomaras, David C. Montefiori, Rafick P. Sekaly, Thomas H. Vanderford, Mark A. Tomai, Christopher B. Fox, Mehul S. Suthar, Pamela A. Kozlowski, Peter J. Hotez, Mirko Paiardini, Maria Elena Bottazzi, Sudhir Pai Kasturi
Summary: The study demonstrates that a yeast-expressed, RBD-based vaccine with 3M-052-alum adjuvant provides strong immune responses and protection against SARS-CoV-2, significantly reducing virus levels in the respiratory tract and lung inflammation. Vaccination also correlates with decreased viral load and plasma analytes, indicating its potential as a scalable vaccine candidate.
SCIENCE IMMUNOLOGY
(2021)
Article
Virology
Ailar Sabbaghi, Masoud Malek, Sara Abdolahi, Seyed Mohammad Miri, Leila Alizadeh, Mehdi Samadi, Seyed Reza Mohebbi, Amir Ghaemi
Summary: The results of the study indicate that co-administration of the gamma-irradiated influenza vaccine with CCL21 or Poly (I:C) can significantly enhance vaccine immunogenicity and improve protective efficacy. Mice receiving the combination of CCL21 with Poly (I:C) showed stronger antibody and cell-mediated immunity, along with reduced inflammatory levels in lung tissue.
Article
Oncology
Hemanth K. Potluri, Tun L. Ng, Michael A. Newton, Douglas G. McNeel
Summary: Antibody responses to off-target cancer-associated proteins may not necessarily indicate antigen spread, especially in vaccine strategies that use GM-CSF as an adjuvant. Evaluating T cell responses to non-target antigens is a preferred approach for detecting immune-mediated antigen spread following immunotherapies.
CANCER IMMUNOLOGY IMMUNOTHERAPY
(2022)
Article
Chemistry, Multidisciplinary
Seckin Akin, Yakup Ulusu, Helen Waller, Jeremy H. Lakey, Savas Sonmezoglu
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2018)
Article
Materials Science, Biomaterials
Gema Dura, Helen Waller, Piergiorgio Gentile, Jeremy H. Lakey, David A. Fulton
MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
(2018)
Article
Multidisciplinary Sciences
Nicolo Paracini, Luke A. Clifton, Maximilian W. A. Skoda, Jeremy H. Lakey
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2018)
Article
Biochemistry & Molecular Biology
Hyea Hwang, Nicolo Paracini, Jerry M. Parks, Jeremy H. Lakey, James C. Gumbart
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2018)
Article
Biochemistry & Molecular Biology
Daniel T. Peters, Laura Kay, Jeyanthy Eswaran, Jeremy H. Lakey, Meera Soundararajan
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2018)
Article
Biochemical Research Methods
Daniel T. Peters, Helen Waller, Mark A. Birch, Jeremy H. Lakey
JOURNAL OF BIOLOGICAL ENGINEERING
(2019)
Article
Biochemistry & Molecular Biology
Yan Huang, Andrei Soliakov, Anton P. Le Brun, Colin Macdonald, Christopher L. Johnson, Alexandra S. Solovyova, Helen Waller, Geoffrey R. Moore, Jeremy H. Lakey
Article
Chemistry, Multidisciplinary
Luke A. Clifton, Nicolo Paracini, Arwel Hughes, Jeremy H. Lakey, Nina-Juliane Steinke, Joshaniel F. K. Cooper, Martynas Gavutis, Maximilian W. A. Skoda
Review
Biochemistry & Molecular Biology
Nicolo Paracini, Luke A. Clifton, Jeremy H. Lakey
BIOCHEMICAL SOCIETY TRANSACTIONS
(2020)
Article
Biophysics
Alexandra S. Solovyova, Daniel T. Peters, Gema Dura, Helen Waller, Jeremy H. Lakey, David A. Fulton
Summary: Caf1 is an oligomeric protein made up of 15 kDa monomeric subunits that can efficiently repolymerize in vitro. The repolymerization process proceeds linearly and is concentration-dependent, with relatively fast polymer growth initially before slowing down dramatically.
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS
(2021)
Article
Microbiology
Daniel T. Peters, Antonio Reifs, Alvaro Alonso-Caballero, Azzeldin Madkour, Helen Waller, Brendan Kenny, Raul Perez-Jimenez, Jeremy H. Lakey
Summary: This study elucidates the mechanism of how F1 protects the pathogenic bacterium Yersina pestis from engulfment by macrophages, by reducing cell adhesion and hiding the innate receptor binding sites required for engulfment.
Article
Biochemistry & Molecular Biology
Gema Dura, Maria Crespo-Cuadrado, Helen Waller, Daniel T. Peters, Ana Ferreira-Duarte, Jeremy H. Lakey, David A. Fulton
Summary: 3D cell culture is superior to 2D in mimicking in vivo environments, and the use of Caf1 hydrogels as a cell culture platform shows promise in avoiding cytotoxicity and enabling successful 3D cell culture.
MACROMOLECULAR BIOSCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Chau Le Bao, Helen Waller, Alessandra Dellaquila, Daniel Peters, Jeremy Lakey, Frederic Chaubet, Teresa Simon-Yarza
Summary: This study aimed to develop 3D porous crosslinked hydrogels with different spatial presentation of pro-angiogenic molecules to guide endothelial cell behavior. The study found that the combined action of two pro-angiogenic proteins was necessary for cell migration, and Caf1-YIGSR provided an adhesive substrate for Caf1-VEGF to be effective. These findings are important for studying pro-angiogenic signals in a 3D environment and developing pro-angiogenic implantable materials.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biophysics
Jeremy H. Lakey, Nicolo Paracini, Luke A. Clifton
Summary: Biological membranes composed of lipids and proteins are essential for cell function. Neutron methods, which can measure structure and dynamics and penetrate samples, are valuable for studying complex molecular mixtures within membranes. This review discusses how contrast can be increased to resolve structures of individual membrane components.
BIOPHYSICS REVIEWS
(2022)
Article
Nanoscience & Nanotechnology
Timothy Robson, Deepan S. H. Shah, Alexandra S. Solovyova, Jeremy H. Lakey
ACS APPLIED NANO MATERIALS
(2018)