期刊
CLINICAL AND VACCINE IMMUNOLOGY
卷 18, 期 9, 页码 1441-1446出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/CVI.00080-11
关键词
-
资金
- Institute for the Promotion of Innovation through Science and Technology in Flanders (IWT-Vlaanderen)
beta-Glucans are well known for their immunomodulatory capacities in humans and mice. For this reason, together with the European ban on growth-promoting antibiotics, beta-glucans are intensively used in pig feed. However, as shown in the present study, there is much variation in the stimulatory capacities of beta-glucans from different sources. Since dendritic cells (DCs) are the first cells that are encountered after an antigen is taken up by the intestinal epithelial cell barrier, we decided to investigate the effect of two concentrations (5 and 10 mu g/ml) of five commercial beta-glucan preparations, differing in structure and source, on porcine monocyte-derived dendritic cells (MoDCs). Although all beta-glucans gave rise to a significant reduction of the phagocytic activity of DCs, only Macrogard induced a significant phenotypic maturation. In addition to Macrogard, zymosan, another beta-glucan derived from Saccharomyces cerevisiae, and curdlan also significantly improved the T-cell-stimulatory capacity of MoDCs. Most interesting, however, is the cytokine secretion profile of curdlan-stimulated MoDCs, since only curdlan induced significant higher expression levels of interleukin-1 beta (IL-1 beta), IL-6, IL-10, and IL-12/IL-23p40. Since the cytokine profile of DCs influences the outcome of the ensuing immune response and thus may prove valuable in intestinal immunity, a careful choice is necessary when beta-glucans are used as dietary supplement.
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