Article
Microbiology
Chunhui Song, Hanze Liu, Zhi Cao, Hu Shan, Qiaoya Zhang
Summary: Heat shock protein 27 (HSP27) is induced and phosphorylated during porcine reproductive and respiratory syndrome virus (PRRSV) infection. Overexpression of HSP27 promotes PRRSV replication, while its knockdown reduces PRRSV proliferation. The phosphorylation of HSP27 is involved in its interaction with viral nonstructural proteins (nsps). In conclusion, HSP27 plays a crucial role in PRRSV replication.
Review
Veterinary Sciences
Yangli Pei, Chenghong Lin, Hua Li, Zheng Feng
Summary: This article provides an overview of the basic infection process, symptoms, immune mechanisms, noncoding RNA, and alternative splicing in PRRSV infection. It emphasizes the significant role of genetic background in susceptibility and resistance to PRRSV and its implications for individual treatment options.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Veterinary Sciences
Peter Oba, Michel M. Dione, Joseph Erume, Barbara Wieland, Christine Mutisya, Linnet Ochieng, Elizabeth A. J. Cook, Frank N. Mwiine
Summary: This study identified the co-circulation of two species of PRRSv in northern Uganda. PRRSv showed an association with lung pathology, suggesting it may be a significant cause of lung disease in pigs in Uganda, leading to production losses. Further investigations are needed to assess the potential economic impacts of PRRSv on pig productivity, and discussions about surveillance and vaccination strategies in Uganda.
BMC VETERINARY RESEARCH
(2022)
Article
Veterinary Sciences
Rui Chen, Bing Liu, Xiangmei Zhang, Mengmeng Qin, Jianhui Dong, Guoqian Gu, Chunyan Wu, Jingyu Wang, Yuchen Nan
Summary: Current strategies for porcine reproductive and respiratory syndrome (PRRS) control are insufficient, and mainly rely on vaccination. This study used a novel monoclonal antibody as a vaccine adjuvant and compared its effectiveness with other vaccines. The results showed that the adjuvanted vaccine significantly improved the survival rate and cellular immune response, and reduced viral shedding.
VETERINARY RESEARCH
(2022)
Review
Microbiology
Xin-xin Chen, Songlin Qiao, Rui Li, Jing Wang, Xuewu Li, Gaiping Zhang
Summary: By studying how PRRSV evades host immune responses, we can understand that the virus establishes long-term infection through various complex mechanisms, which is a major obstacle in controlling PRRS disease. Understanding the exact mechanisms of PRRSV immune evasion will contribute to the development of novel antiviral strategies.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Veterinary Sciences
Giulia D'Annunzio, Fabio Ostanello, Luisa Vera Muscatello, Massimo Orioles, Niccolo Jacumin, Nicola Tommasini, Giorgio Leotti, Andrea Luppi, Luciana Mandrioli, Giuseppe Sarli
Summary: This study examines the impact of Porcine Circovirus type 2 (PCV2) and Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) on Porcine Respiratory Disease Complex (PRDC). The results show that PCV2 and PRRSV are the primary infections associated with PRDC in Italy, and co-infections can worsen the pathology. Therefore, selecting appropriate preventive measures is crucial.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Immunology
Loic Vivien Bocard, Andrew Robert Kick, Corinne Hug, Heidi Erika Lisa Lischer, Tobias Kaser, Artur Summerfield
Summary: This study aimed to understand the nature of innate and adaptive immune responses to PRRSV, revealing differences in regulation by PRRSV-2 strains and MLV. Different PRRSV strains induced varied immune responses, impacting platelet activation, dendritic cell activity, interferon type I, and plasma cell responses. Correlations of BTMs with T-cell responses showed both positive and negative associations post-infection, indicating complexities in immune regulation.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Virology
Shengnan Ruan, Wenhui Ren, Bin Yu, Xuexiang Yu, Hao Wu, Wentao Li, Yunbo Jiang, Qigai He
Summary: In this study, a new TaqMan-based RT-qPCR assay was developed to detect and type the circulating PRRSV strains in Chinese swine populations. The assay showed high specificity, sensitivity, and concordance with reference assays, and could assist in monitoring PRRSV prevalence and implementing prevention and control strategies effectively.
Article
Biochemistry & Molecular Biology
Guangwei Han, Haotian Yang, Huiling Xu, Suya Zheng, Yingxiang Li, Zehui Liu, Fang He
Summary: Novel antiviral peptides derived from pCD163 were developed, which showed inhibitory effects on PRRSV of different lineages and modulated the NF-kappa B pathway. These findings deepen the understanding of the interaction between PRRSV and pCD163 receptor, and provide alternative universal antiviral strategies against PRRSV.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Review
Virology
Jessica Risser, Matthew Ackerman, Robert Evelsizer, Stephen Wu, Byungjoon Kwon, James Mark Hammer
Summary: With the decreasing cost of genetic analysis, veterinary practitioners will have access to more comprehensive diagnostics like whole genome sequencing (WGS). Understanding the mechanics and consequences of PRRSV genetic variability is becoming increasingly important, as recombination events and mutations continuously impact swine populations. Controlling PRRSV and managing its impact on swine populations requires a well-defined understanding of genetic diversity and the factors influencing recombination.
Article
Immunology
Yuening Cheng, Miaoli Wu, Li Xiao, Mengdi Zhang, Bihong Huang, Feng Cong, Li Yi
Summary: In this study, a specific monoclonal antibody (mAb N06) against porcine reproductive and respiratory syndrome virus (PRRSV) was identified to recognize the linear epitope NRKKNPEKPHFPLATE. This antibody can be used as a diagnostic reagent for PRRSV detection and can also be helpful for the development of epitope-based vaccines to control PRRSV infections in swine.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Microbiology
Dike Jiang, Teng Tu, You Zhou, Yanwei Li, Yan Luo, Xueping Yao, Zexiao Yang, Meishen Ren, Yin Wang
Summary: This study investigated the PRRSV epidemic situation in Sichuan province, China, and identified HP-PRRSV as the predominant strain. A new PRRSV strain named SCSN2020 was isolated and characterized, showing high genomic similarity to the JXA1 strain. The study also demonstrated the pathogenicity of the SCSN2020 strain in piglets.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Feng Long, Yating Chen, Kaichuang Shi, Yanwen Yin, Shuping Feng, Hongbin Si
Summary: PRRSV type 1 (European genotype) and PRRSV type 2 (North American genotype) are prevalent in China, and classical PRRSV (C-PRRSV), highly pathogenic PRRSV (HP-PRRSV), and NADC30-like PRRSV (NL-PRRSV) are the most common circulating strains. Multiplex real-time quantitative RT-PCR and multiplex Crystal digital RT-PCR were developed for the differential detection of these strains and showed high sensitivity, strong specificity, and excellent repeatability.
Article
Microbiology
Rong Wang, Yueqiang Xiao, Qian Zhang, Liang Bai, Weirong Wang, Sihai Zhao, Enqi Liu
Summary: This study demonstrated that highly pathogenic PRRSV strain HuN4 induced significant secretion of HMGB1 and inflammatory cytokines in the bronchoalveolar lavage fluid (BALF), resulting in severe interstitial pneumonia in pigs. In contrast, infection with an avirulent PRRSV vaccine strain CH1R led to mild lung inflammation with minimal HMGB1 secretion.
VETERINARY MICROBIOLOGY
(2021)
Article
Virology
Qiyun Ke, Kaiqi Duan, Yan Cheng, Si Xu, Shaobo Xiao, Liurong Fang
Summary: Porcine reproductive and respiratory syndrome virus (PRRSV) is a worldwide prevalent virus that causes significant economic losses to the swine industry. Current vaccines fail to effectively control PRRSV, therefore the development of safe and effective antiviral drugs is urgently needed. Sanguinarine, a natural alkaloid found in many plants including Macleaya cordata, has been demonstrated as a potent antagonist against PRRSV. It targets multiple stages of the viral life cycle and shows promising potential as a novel candidate for anti-PRRSV agents.
Article
Biotechnology & Applied Microbiology
Peiyang Li, Jacek A. Koziel, Jeffrey J. Zimmerman, Steven J. Hoff, Jianqiang Zhang, Ting-Yu Cheng, Wannarat Yim-Im, Myeongseong Lee, Baitong Chen, William S. Jenks
Summary: This study built and tested a virus aerosolization system, ensuring uniform and repeatable aerosol generation and collection through effective control of flow rates and airflow distribution, while verifying impinger performance. The results showed that the system is ready for research on virus aerosolization and treatment, with potential for laboratory-scale research on other types of airborne pathogens.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Veterinary Sciences
Edison S. Magalhaes, Jeffrey J. Zimmerman, Pete Thomas, Cesar A. A. Moura, Giovani Trevisan, Derald J. Holtkamp, Chong Wang, Christopher Rademacher, Gustavo S. Silva, Daniel C. L. Linhares
Summary: This study developed an automated framework to analyze the risk factors for swine wean-to-finish mortality by integrating data from multiple sources. The results showed that sow farm health and productivity were closely linked to subsequent mortality during the wean-to-finish phase.
PREVENTIVE VETERINARY MEDICINE
(2022)
Article
Microbiology
Karyn A. Havas, Andrey E. Gogin, Julia V. Basalaeva, Irina P. Sindryakova, Olga L. Kolbasova, Ilya A. Titov, Valentina M. Lyska, Sergey Y. Morgunov, Mikhail E. Vlasov, Timofey A. Sevskikh, Elena Y. Pivova, Dmitry A. Kudrjashov, Kent Doolittle, Silvia Zimmerman, Wendy Witbeck, Luis G. Gimenez-Lirola, Joel Nerem, Gordon D. Spronk, Jeffrey J. Zimmerman, Alexey D. Sereda
Summary: This study focused on the diagnostic detection of attenuated African swine fever virus. The results showed that early detection requires active surveillance in healthy pigs, and it is only reliable at the herd level.
Correction
Agronomy
Peiyang Li, Jacek A. Koziel, Jeffrey J. Zimmerman, Jianqiang Zhang, Ting-Yu Cheng, Wannarat Yim-Im, William S. Jenks, Myeongseong Lee, Baitong Chen, Steven J. Hoff
Article
Veterinary Sciences
Ana Paula S. Poeta Silva, Gabriel Y. Storino, Franco S. Matias Ferreyra, Min Zhang, Jessica M. Miller, Karen M. Harmon, Phillip C. Gauger, Wendy Witbeck, Kent Doolittle, Silvia Zimmerman, Chong Wang, Rachel J. Derscheid, Maria J. Clavijo, Bailey L. Arruda, Jeffrey J. Zimmerman
Summary: The probability of detecting Mycoplasma hyopneumoniae (MHP) DNA in oral fluid samples was compared using different nucleic acid extractions and PCR protocols. The study found that the probability of detection varied among test protocols and was positively associated with within-pen MHP prevalence. This suggests that improvements in extraction methods and MHP PCR are possible, and the dynamics of MHP infections should be considered when using oral fluid samples for surveillance.
PREVENTIVE VETERINARY MEDICINE
(2022)
Article
Microbiology
Lu Yen, Ronaldo Magtoto, Juan Carlos Mora-Diaz, Jose Antonio Carrillo-Avila, Jianqiang Zhang, Ting-Yu Cheng, Precy Magtoto, Rahul K. Nelli, David H. Baum, Jeffrey J. Zimmerman, Luis G. Gimenez-Lirola
Summary: This study evaluated the potential of using the receptor-binding subunit of the PDCoV spike protein for specific antibody detection via an ELISA assay. The results showed that the S1 protein-based ELISA can be used for accurate detection of PDCoV infections, transfer of maternal antibodies, or active surveillance.
Article
Virology
Lu Yen, Juan Carlos Mora-Diaz, Rolf Rauh, William Nelson, Gino Castillo, Fangshu Ye, Jianqiang Zhang, David Baum, Jeffrey Zimmerman, Rahul Nelli, Luis Gimenez-Lirola
Summary: This study characterized the susceptibility and dynamic of porcine deltacoronavirus infection in grower pigs under experimental conditions. The study found that active surveillance based on systematic sampling and laboratory testing is critical for the accurate detection of subclinical circulation of PDCoV in pigs after weaning.
Article
Microbiology
Virginia Friedrichs, Darwin Reicks, Tobias Hasenfuss, Elisabeth Gerstenkorn, Jeffrey J. Zimmerman, Eric A. Nelson, Tessa Carrau, Paul Deutschmann, Julia Sehl-Ewert, Hanna Roszyk, Martin Beer, Jane Christopher-Hennings, Sandra Blome
Summary: African swine fever virus (ASFV) can efficiently spread through artificial insemination to female pigs and also to implanted embryos, highlighting the critical role that boar semen plays in ASFV transmission.
Article
Veterinary Sciences
Berenice Munguia-Ramirez, Betsy Armenta-Leyva, Alexandra Henao-Diaz, Ting-Yu Cheng, Jianqiang Zhang, Gaurav Rawal, Fangshu Ye, Luis Gimenez-Lirola, Jeffrey J. Zimmerman
Summary: We investigated the effects of temperature x time, freeze-thaw cycles, and high PRRSV RNA concentrations on the detection of PRRSV and ISC in different sample types. Serum samples should be stored at <=20 degrees C for optimal PRRSV RNA detection, while oral fluid and fecal samples should be frozen until tested. Freeze-thaw cycles had little impact on detection, but should be minimized in oral fluid samples.
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION
(2023)
Article
Veterinary Sciences
Betsy Armenta-Leyva, Berenice Munguia-Ramirez, Luis G. Gimenez-Lirola, Xue Lin, Fangshu Ye, Jeffrey Zimmerman
Summary: Based on publications reporting improvements in real-time PCR (rtPCR) performance, this study compared different protocols for the detection of specific viruses in swine oral fluids (OFs). The results showed that standard extraction and amplification methods provided superior detection of the viruses compared to heat treatment or dilution followed by direct rtPCR.
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION
(2023)
Article
Veterinary Sciences
Berenice Munguia-Ramirez, Betsy Armenta-Leyva, Alexandra Henao-Diaz, Fangshu Ye, David H. Baum, Luis G. Gimenez-Lirola, Jeffrey J. Zimmerman
Summary: This study aimed to evaluate the consistency of endogenous reference gene expression and their interpretation in diagnostic specimens. Results showed that the reference gene can accurately monitor sample quality in swine specimens tested for PRRSV. Failure to detect the reference gene may indicate abnormalities in the sample or testing procedures.
VETERINARY SCIENCES
(2023)
Article
Veterinary Sciences
Betsy Armenta-Leyva, Berenice Munguia-Ramirez, Ting-Yu Cheng, Fangshu Ye, Alexandra Henao-Diaz, Luis G. Gimenez-Lirola, Jeffrey Zimmerman
Summary: This study demonstrates the use of a normalization method in real-time PCR assays to control for normal variation in sampling and testing. The results show that converting sample quantification cycles to efficiency standardized cycles improves the accuracy of the assay. The method is shown to have good diagnostic capability in commercial porcine reproductive and respiratory syndrome virus testing.
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION
(2023)
Article
Veterinary Sciences
Menghang Wang, Ying Yu, Jianan Wu, Shujie Wang, Luis G. Gimenez-Lirola, Pablo Pineyro, Yu Wang, Hongliang Cui, Xijun He, Jeffrey J. Zimmerman, Yabin Tu, Xuehui Cai, Gang Wang
Summary: In this study, researchers isolated and characterized the PCV3-DB-1 strain in PK-15 cells. They observed PCV-like particles through electron microscopy and confirmed the replication of PCV3 in PK-15 cell culture using in situ hybridization RNA analysis. Phylogenetic analysis placed PCV3-DB-1 in the PCV3a clade based on its Cap protein characteristics.
VETERINARY SCIENCES
(2023)
Article
Virology
Gaurav Rawal, Marcelo N. Almeida, Phillip C. Gauger, Jeffrey J. Zimmerman, Fangshu Ye, Christopher J. Rademacher, Betsy Armenta Leyva, Berenice Munguia-Ramirez, Grzegorz Tarasiuk, Loni L. Schumacher, Ethan K. Aljets, Joseph T. Thomas, Jin-Hui Zhu, Jolie B. Trexel, Jianqiang Zhang
Summary: This study characterized the recently emerged PRRSV 1-4-4 L1C variant (L1C.5) and found it to be highly virulent with potential higher transmissibility. Compared to other virus strains, pigs infected with L1C.5 showed more severe symptoms and higher viral levels. Additionally, the study found that some PRRSV isolates can cause brain infection.
Article
Environmental Sciences
Peiyang Li, Jacek A. A. Koziel, Nubia Macedo, Jeffrey J. J. Zimmerman, Danielle Wrzesinski, Erin Sobotka, Mateo Balderas, William B. B. Walz, Reid Vincent Paris, Myeongseong Lee, Dongjie Liu, Bauyrzhan Yedilbayev, Brett C. C. Ramirez, William S. S. Jenks
Summary: Improving indoor air quality has become crucial during the COVID-19 pandemic. Research shows that adding UV-C light to air filtration devices can effectively eliminate viable airborne bacteria and remove particulate matter.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)