Article
Chemistry, Analytical
Natalie Tran, Priyanka Shiveshwarkar, Justyn Jaworski
Summary: This study demonstrates a proof-of-concept assay to detect the presence of antibodies by creating vesicles with peptide epitopes that can bind specifically to the antibodies. The vesicles visibly aggregate in response to the specific antibodies, and the density of the peptide epitopes on the vesicles plays a role in antibody binding.
Article
Microbiology
Huan Huang, Rong Qu, Kang Wu, Jinchuan Xu, Jianhui Li, Shuihua Lu, Guodong Sui, Xiao-Yong Fan
Summary: This study developed a proteinase K-pretreated ConA-based ELISA assay to detect LAM. The method could distinguish TB patients from healthy controls and improve sensitivity through proteinase K treatment.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Anneliese S. Ashhurst, Matt D. Johansen, Joshua W. C. Maxwell, Skye Stockdale, Caroline L. Ashley, Anupriya Aggarwal, Rezwan Siddiquee, Stefan Miemczyk, Duc H. Nguyen, Joel P. Mackay, Claudio Counoupas, Scott N. Byrne, Stuart Turville, Megan Steain, James A. Triccas, Philip M. Hansbro, Richard J. Payne, Warwick J. Britton
Summary: Current vaccines against SARS-CoV-2 reduce mortality but are less effective in preventing infection. In this study, the authors demonstrate that intranasal vaccination with a subunit vaccine containing a TLR2-stimulating adjuvant can induce strong neutralizing antibody and T-cell responses against SARS-CoV-2 in the lungs, providing protection against infection. This highlights the potential of mucosal vaccination to improve protection in the respiratory tract against SARS-CoV-2.
NATURE COMMUNICATIONS
(2022)
Article
Chemistry, Analytical
Bareum Kil, Eunseon Kim, Minji Kang, Jinseong Jeon, Yunhee Chang, Chulhun L. Chang, Changill Ban
Summary: This study developed an aptamer-based qPCR assay for the safe and rapid diagnosis of tuberculosis. The assay used a DNA aptamer to target the TB biomarker ESAT6, and achieved high reproducibility and reliability. In clinical application, the assay showed similar sensitivity, specificity, and accuracy to sputum smear microscopy, with the advantage of reducing the risk of infection.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Multidisciplinary Sciences
Rutwik Gulakala, Bernd Markert, Marcus Stoffel
Summary: This study proposes an AI-based rapid diagnostic tool for Covid infections using generative adversarial and convolutional neural networks. It achieves high accuracy in identifying lung infections based on X-ray images. The method provides a reliable and efficient way to determine the presence of Covid infections.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Erlend Gronningen, Marywinnie Nanyaro, Lisbet Sviland, Esther Ngadaya, William Muller, Lisete Torres, Sayoki Mfinanga, Tehmina Mustafa
Summary: Pediatric extrapulmonary tuberculosis diagnosis can be challenging. A new immunochemistry based MPT64 antigen detection test showed improved sensitivity compared to current tests. The study aimed to implement and validate this test in a resource limited African setting, demonstrating its effectiveness in improving diagnosis accuracy.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Medicinal
Xinggui Yang, Xu Chen, Junfei Huang, Yijiang Chen, Wenlin Zheng, Wei Chen, Huijuan Chen, Shiguang Lei, Shijun Li
Summary: A simple, specific, low-cost, and highly sensitive detection method for Mycobacterium tuberculosis (MTB) was developed using multiplex loop-mediated isothermal amplification combined with a label-based lateral flow immuno-assay biosensor (mLAMP-LFIA). The mLAMP-LFIA method, targeting the IS6110 and mpb64 genes, showed a detection limit of 100 fg per reaction for MTB DNA, and a sensitivity of 100% and specificity of 98.04% in preliminary clinical evaluations. This method has the potential to be used as a screening and/or diagnostic tool for MTB in various settings.
ACS INFECTIOUS DISEASES
(2023)
Article
Biophysics
Anh Thi Viet Nguyen, Bao Tuan Duong, Hyun Park, Seon-Ju Yeo
Summary: A novel peptide pair-based flow immunochromatographic test strip was developed to detect Zika virus (ZIKV) with a limit of detection of 2 x 10^4 TCID50/mL, and the test could distinguish ZIKV from Dengue virus (DENV) with high stability and performance in human sera and urine. This study demonstrates the potential of using in silico modeling to develop rapid diagnostic tests for detecting ZIKV.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Immunology
Yuanyuan Zhou, Huan Xiong, Rong Chen, Lixia Wan, Ying Kong, Jianwei Rao, Yan Xie, Chaolin Huang, Xiao-Lian Zhang
Summary: The study aimed to establish an immunohistochemistry (IHC) method for TB diagnosis based on M.tb ManLAM aptamer, showing high sensitivity and specificity, and further enhancement of sensitivity when combined with routine TB laboratory diagnostic methods.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Patricia Comella-del-Barrio, Jose Luis Izquierdo-Garcia, Jacqueline Gautier, Mariette Jean Coute Doresca, Ramon Campos-Olivas, Clara M. Santiveri, Beatriz Muriel-Moreno, Cristina Prat-Aymerich, Rosa Abellana, Tomas M. Perez-Porcuna, Luis E. Cuevas, Jesus Ruiz-Cabello, Jose Dominguez
Summary: In this study, differences in metabolic response in children with different diagnostic certainty of TB were identified using urine nuclear magnetic resonance-based metabolomics. This could help improve the accuracy of TB diagnosis and prediction in pediatric populations.
SCIENTIFIC REPORTS
(2021)
Article
Electrochemistry
Sasinee Bunyarataphan, Therdsak Prammananan, Deanpen Japrung
Summary: A ratiometric electrochemical biosensor was developed for detection of Mycobacterium tuberculosis (MTB), the causative agent of tuberculosis. The incorporation of internal control detection minimized false negative results, and the biosensor exhibited high accuracy and practicality in practical samples.
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
(2022)
Review
Immunology
Wenping Gong, Chao Pan, Peng Cheng, Jie Wang, Guangyu Zhao, Xueqiong Wu
Summary: Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis. The COVID-19 pandemic has led to an increase in global TB mortality rate, necessitating the development of more effective TB vaccines. Peptides have been found to play important roles in immune response. This review provides an overview of tools for designing peptide-based vaccines, the current research status of such vaccines for TB, protein-based bacterial vaccine delivery systems, and animal models for peptide-based vaccines.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Radiology, Nuclear Medicine & Medical Imaging
Chenggong Yan, Lingfeng Wang, Jie Lin, Jun Xu, Tianjing Zhang, Jin Qi, Xiangying Li, Wei Ni, Guangyao Wu, Jianbin Huang, Yikai Xu, Henry C. Woodruff, Philippe Lambin
Summary: This study developed an AI-based fully automated CT image analysis system for detection, diagnosis, and burden quantification of pulmonary TB, achieving human-level diagnostic performance through deep learning technology.
EUROPEAN RADIOLOGY
(2022)
Article
Biochemical Research Methods
Dan Ren, Xinyun Zhang, Wei Zhang, Mingjun Lian, Xianchen Meng, Tuofan Li, Quan Xie, Hongxia Shao, Zhimin Wan, Aijian Qin, Wei Gao, Jianqiang Ye
Summary: A peptide-based ELISA method was developed for the detection of antibodies against GAstV-1. The pELISA showed high specificity and sensitivity in detecting positive sera, and had good reproducibility. It also had a high concordance rate with the IFA for clinical sample detection.
JOURNAL OF VIROLOGICAL METHODS
(2023)
Article
Chemistry, Analytical
Muhammad Hafiznur Yunus, Nor Azah Yusof, Jaafar Abdullah, Yusran Sulaiman, Nurul Hanun Ahmad Raston, Siti Suraiya Md Noor
Summary: A novel dual aptasensor was developed for the simultaneous detection of Mycobacterium tuberculosis secreted antigens CFP10 and MPT64, improving the diagnosis and control of TB.