Article
Medicine, Research & Experimental
You Zhuo, David A. Keire, Kang Chen
Summary: The N-glycosylation pattern of Asn-297 can impact mAb drug plasma clearance, ADCC, and CDC. Using a middle-down NMR spectroscopy based analytical procedure, the composition and structure of glycans on adalimumab and trastuzumab were assessed without glycan cleavage. The obtained information is relevant for assessing mAb quality attributes.
MOLECULAR PHARMACEUTICS
(2021)
Review
Chemistry, Multidisciplinary
Ignacio Sanz-Martinez, Sandra Pereira, Pedro Merino, Francisco Corzana, Ramon Hurtado-Guerrero
Summary: N-acetylgalactosamine (GalNAc) type O-glycosylation is a crucial posttranslational modification in biology. Malfunction of this modification is associated with truncated O-glycans in cancer. Understanding the structure and recognition of GalNAc-containing glycopeptides can contribute to the development of therapeutic vaccines and new diagnostic tools for cancer detection.
ACCOUNTS OF CHEMICAL RESEARCH
(2023)
Article
Chemistry, Multidisciplinary
Shaomin Lin, Zeyuan Mo, Peng Wang, Chunmao He
Summary: In order to meet the demand for peptide salicylaldehyde esters, we developed a new method for the preparation of these esters from peptide hydrazides through nitrite oxidation and phenolysis. This method is simple and suitable for both synthetic peptides and expressed proteins, facilitating peptide synthesis and the combined application of NCL and STL.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Multidisciplinary Sciences
Yang Han, Lin Zhong, Fuli Ren
Summary: This study established a simple and broad-spectrum system for preparing phosphorylation-positive samples, which were then validated in cells from different species and tissues, demonstrating effectiveness in evaluating the quality of phosphorylation-specific antibodies.
Article
Multidisciplinary Sciences
Yang Han, Lin Zhong, Fuli Ren
Summary: This study established a simple and broad-spectrum system for evaluating the quality of phosphorylation-specific antibodies, which was validated in multiple experiments and shown to be effective and applicable in various biomedical fields.
Article
Multidisciplinary Sciences
Andrea Piserchio, Kimberly J. Long, Luke S. Browning, Amanda L. Bohanon, Eta A. Isiorho, Kevin N. Dalby, Ranajeet Ghose
Summary: Protein translation, a high-energy process, requires robust regulation under energy-deprived conditions. The eEF-2K plays a crucial role in this regulation by suppressing translational elongation through phosphorylation of eEF-2. It has been suggested that eEF-2K's response to energy levels is mediated by AMPK and the mTOR pathway.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biotechnology & Applied Microbiology
Anuja Prabhu, Dhanasekaran Shanmugam, Mugdha Gadgil
Summary: This study demonstrates the ability to predictably modulate the glycoform profile of recombinant IgG by engineering nucleotide sugar synthesis pathways in CHO cells. By knocking out specific enzymes, the researchers were able to narrow down the array of N-glycans and control the galactosylation and fucosylation levels of the recombinant IgG.
METABOLIC ENGINEERING
(2022)
Article
Biochemistry & Molecular Biology
Zhi-Da Zhang, Bing Wen, Da-Jia Li, Dan-Xia Deng, Xiao-Dong Wu, Yin-Wei Cheng, Lian-Di Liao, Lin Long, Geng Dong, Li-Yan Xu, En-Min Li
Summary: In this study, we identified a novel phosphorylation site in Fascin and confirmed AKT2 as the kinase for this site. Phosphorylation of Fascin Threonine 403 suppresses the behavior of esophageal cancer cells, actin-bundling activity, and filopodia formation.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2022)
Review
Pharmacology & Pharmacy
Soghra Bagheri, Mahdie Rahban, Fatemeh Bostanian, Fatemeh Esmaeilzadeh, Arash Bagherabadi, Samaneh Zolghadri, Agata Stanek
Summary: Prostate cancer, when it spreads to the bone, is known as metastatic bone cancer. Currently, effective treatment options are still lacking for advanced/metastatic PC. However, understanding the molecular events that contribute to PC progression provides hope for the development of new treatment strategies. Protein kinases play crucial roles in the growth, proliferation, and metastases of prostatic tumors, making combinatorial therapy with PK inhibitors a potential treatment approach. Additionally, epigenetic pathways are also involved in PC, suggesting another potential combination treatment strategy.
Article
Multidisciplinary Sciences
Dhanashree Lokesh, P. S. N. Suresh, Rajagopal Kammara
Summary: This article describes the signal transduction pathway of prokaryotes, involving a peptidoglycan synthesis cluster and protein kinases. The authors developed a data repository of probiotic eSTKs, containing 830 STK sequences. This resource can be useful for sequence-based studies and drug development.
Article
Multidisciplinary Sciences
Vadim M. Gumerov, Ekaterina P. Andrianova, Miguel A. Matilla, Karen M. Page, Elizabet Monteagudo-Cascales, Annette C. Dolphin, Tino Krell, Igor B. Zhulin
Summary: This study identifies a common amino acid recognition mechanism in bacteria, archaea, and humans, which may lead to improvements in drug targeting pain and neurobiological disorders.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Hua Tang, Qiang Tang, Qian Zhang, Pengmian Feng
Summary: This study constructed a prediction model based on feature fusion to predict O-glycosites linked to threonine residues in human proteins. Random forest was selected as the final classifier and achieved high accuracy on both training and validation datasets. The developed webserver, O-GlyThr, assists glycobiologists in researching glycosylation structure and function.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Cell Biology
Mirabela Hali, Brian E. Wadzinski, Anjaneyulu Kowluru
Summary: This study investigated the role of Alpha4, a non-canonical subunit of protein phosphatase 2A, in the regulation of glucose-induced acute and chronic effects in pancreatic beta cells. Alpha4 expression was increased in response to high glucose and C2-Ceramide. Knockdown of Alpha4 attenuated ER stress and cell death induced by high glucose, while preventing the decrease in Connexin36 expression. These findings suggest that Alpha4 contributes to metabolic dysfunction and cell death in pancreatic beta cells under high glucose conditions.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2022)
Article
Microbiology
Qiao Hu, Lun Yao, Xia Liao, Liang-Sheng Zhang, Hao-Tian Li, Ting-Ting Li, Qing-Gen Jiang, Mei-Fang Tan, Lu Li, Roger R. Draheim, Qi Huang, Rui Zhou
Summary: A comparative study on STK/STP in Streptococcus suis revealed that STK plays a more critical role in cell division, energy metabolism, and protein translation, while STP primarily impacts bacterial growth. Deletion of stk or stp had significant effects on protein expression, with more virulence factors downregulated in increment stk strains. Overall, both STP and STK are essential regulatory proteins for S. suis, with STK playing a more prominent role in growth, cell division, and pathogenesis.
Article
Endocrinology & Metabolism
Michael A. Swanson, Kristen Miller, Sarah P. Young, Suhong Tong, Lina Ghaloul-Gonzalez, Juanita Neira-Fresneda, Lisa Schlichting, Cheryl Peck, Linda Gabel, Marisa W. Friederich, Johan L. K. Van Hove
Summary: Changes in serine and threonine levels in nonketotic hyperglycinemia (NKH) patients are related to the severity of clinical outcomes. This finding provides additional guidance for diagnosis and prognosis, as well as new therapeutic opportunities.
JOURNAL OF INHERITED METABOLIC DISEASE
(2022)