Article
Chemistry, Applied
Weifeng Lin, Kiana Mellinghaus, Ana Rodriguez-Mateos, Daniel Globisch
Summary: Researchers have developed a mass spectrometric methodology to analyze carbonyl metabolites in urine samples related to diet. They have successfully identified 30 carbonyl metabolites of dietary origin and discovered four unknown dietary markers. This method provides the basis for large-scale analysis and identification of diet-related biomarkers.
Review
Biochemistry & Molecular Biology
Montse Baldan-Martin, Maria Chaparro, Javier P. Gisbert
Summary: Inflammatory bowel diseases (IBDs) are chronic and inflammatory conditions affecting the gastrointestinal tract. Despite endoscopy being the gold standard test for assessing IBD, it is invasive and uncomfortable for patients. Therefore, non-invasive biomarkers for IBD diagnosis are urgently needed. This review summarizes proteomics and metabolomics studies in animals and humans, identifying urinary biomarkers for IBD diagnosis. Large-scale multi-omics studies with collaboration between clinicians, researchers, and industry are necessary for the development of sensitive and specific diagnostic biomarkers for personalized medicine.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Food Science & Technology
Eirini Trichia, Albert Koulman, Isobel D. Stewart, Soren Brage, Simon J. Griffin, Julian L. Griffin, Kay-Tee Khaw, Claudia Langenberg, Nicholas J. Wareham, Fumiaki Imamura, Nita G. Forouhi
Summary: The study identified metabolites associated with habitual dairy consumption and found that consuming milk, butter, and total dairy products is associated with lower risk of type 2 diabetes.
MOLECULAR NUTRITION & FOOD RESEARCH
(2023)
Article
Chemistry, Analytical
Yanqing Song, Jie Ma, Huan Gao, Jinghui Zhai, Yueming Zhang, Jiawei Gong, Xiaoyu Qu, Tingting Hu
Summary: In this study, HPLC-TOF/MS-based untargeted metabolomics was used to analyze urine samples from a mouse model to investigate the mechanisms of hepatotoxicity and neurotoxicity induced by the co-administration of isoniazid and rifampicin. The analysis revealed disruptions in various metabolic pathways, including vitamin B6 metabolism, unsaturated fatty acid biosynthesis, and the metabolism of various amino acids and other compounds. Several urinary biomarkers were identified as potential diagnostic and evaluation tools for toxicity.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Endocrinology & Metabolism
Yuwei Tan, Xiaosong Liu, Yinping Yang, Baoying Li, Fei Yu, Wenqian Zhao, Chunli Fu, Xin Yu, Zhenxia Han, Mei Cheng
Summary: Diabetic sarcopenia (DS) is a disease characterized by muscle atrophy, slower nerve conduction, reduced muscle contraction capacity, and slower contraction rate. Serum metabolomics analysis identified potential biomarkers and revealed the underlying pathophysiological mechanism of DS. These findings provide new insights for the treatment of DS.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Zhangjian Chen, Shuo Han, Jiahe Zhang, Pai Zheng, Xiaodong Liu, Yuanyuan Zhang, Guang Jia
Summary: This study identified potential biomarkers of occupational exposure to low concentrations of TiO2 NPs in urine samples of workers through metabolomics analysis. A total of 1760 differentially expressed metabolites were found, with 60 confirmed as potential biomarkers. Among these biomarkers, 10 showed good sensitivity and specificity, including compounds like dibenzyl ether and quassimarin. Pathway enrichment analysis revealed significant alterations in the metabolic pathway related to long chain acyl-coa dehydrogenase deficiency. These findings provide insights into early health effects of TiO2 NPs exposure.
Article
Biochemistry & Molecular Biology
Petros Pousinis, Christina Virgiliou, Thomai Mouskeftara, Sofia Chalvatzi, Fotios Kroustallas, Eleftherios Panteris, Georgios A. A. Papadopoulos, Paschalis Fortomaris, Michaela Cernat, Leonidas Leontides, Olga Begou
Summary: Urinary tract infections (UTI) of sows are major health issues in the swine industry, but current detection methods lack sensitivity. This study investigated the urine metabolome of sows to identify UTI biomarkers. Eighteen potential markers were found, and these biomarkers showed good predictive accuracy for UTI. This study provides new information for early and accurate diagnosis of UTI and sheds light on their application in live animals.
Article
Medicine, General & Internal
Soumaya Kouidhi, Nessrine Souai, Muhanad Alhujaily, Oumaima Zidi, Ameni Kochbati, Alaeddine Redissi, Tareg M. Belali, Imene El Kossai, Jamelddine El Manaa, Ameur Cherif, Wissem Mnif, Amor Mosbah
Summary: The study conducted untargeted fecal metabolomic analysis to investigate the metabolic differences between stable kidney allograft recipients and control group. The results showed distinct metabolic profiles affecting fatty acid biosynthesis and amino acid metabolism, providing new relevant biomarkers for preventing kidney transplant complications.
Article
Chemistry, Analytical
Jung Bin Phyo, Ayoung Woo, Ho Jae Yu, Kyongmook Lim, Baek Hwan Cho, Ho Sang Jung, Min-Young Lee
Summary: Metabolomics has shown great potential for early cancer diagnosis, and a rapid urine analysis system based on SERS has been developed for the diagnosis of pancreatic cancer and prostate cancer. The results indicate that the system has great potential for noninvasive cancer diagnosis and screening.
ANALYTICAL CHEMISTRY
(2021)
Article
Pharmacology & Pharmacy
Yin Yuan, Fei-Xue Dong, Xu Liu, Hong-Bin Xiao, Zhong-Guang Zhou
Summary: This study revealed that Magnoflorine has an intervention effect on prostatitis, inhibiting its development by regulating metabolic pathways and biomarkers. The results suggest that alkaloids could be an effective therapy for prostatitis, with specific metabolic pathways closely related to the condition identified.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Gabriel A. Kurokawa, Pedro T. Hamamoto Filho, Jeany Delafiori, Aline F. Galvani, Arthur N. de Oliveira, Flavia L. Dias-Audibert, Rodrigo R. Catharino, Maria Ines M. C. Pardini, Marco A. Zanini, Estela de O. Lima, Adriana C. Ferrasi
Summary: This study identified plasma metabolic alterations that can differentiate between low-grade and high-grade meningiomas, providing valuable information for surgeons to determine the ideal surgical strategy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Public, Environmental & Occupational Health
Tianqi Li, Andrei Ihanus, Pauli Ohukainen, Marjo-Riitta Jarvelin, Mika Kahonen, Johannes Kettunen, Olli T. Raitakari, Terho Lehtimaki, Ville-Petteri Makinen, Tuulia Tynkkynen, Mika Ala-Korpela
Summary: Urinary metabolomics has the potential to assess kidney function and its metabolic corollaries. With the addition of metabolites and automated quantification, this study presents associations between metabolites and clinical measures, providing insights in renal-cardiometabolic system and supporting its application in epidemiology.
INTERNATIONAL JOURNAL OF EPIDEMIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Yongpeng Shi, Jiande Li, Ji Wang, Hanwen Cao, Huanbing Tian, FeiFei Yu, Lan Gao
Summary: This study investigated the mechanism of COL-induced diarrhea by exposing mice to COL. The results showed that COL exposure disrupted intestinal metabolic homeostasis, resulting in accumulation or depletion of various metabolites. Certain core metabolites were found to be closely related to COL-induced diarrhea and could be used as biomarkers. Changes in metabolic pathways and abnormal bile secretion were identified as important factors in COL-induced diarrhea.
CHEMICO-BIOLOGICAL INTERACTIONS
(2022)
Review
Biochemistry & Molecular Biology
John Oloche Onuh, Hongyu Qiu
Summary: Detecting pathogenesis early through metabolomics profiling offers new insights on disease mechanisms and potential in diagnosing clinical hypertension early. This review summarizes the latest progress in metabolomics and discusses current trends in applying it to clinical hypertension, exploring associated mechanisms and research challenges for future perspectives. The information enhances understanding of hypertension development and encourages clinical application of metabolomics in hypertension and cardiovascular complications.
Article
Biochemistry & Molecular Biology
Gaurav Baranwal, Rachel Pilla, Bethany L. Goodlett, Aja K. Coleman, Cristina M. Arenaz, Arul Jayaraman, Joseph M. Rutkowski, Robert C. Alaniz, Brett M. Mitchell
Summary: Recent metabolomics studies have identified a wide array of microbial metabolites and metabolite pathways that are significantly altered in hypertension. Common changes in serum metabolites were found in experimental hypertension models, but not in urine metabolites, indicating the potential association between gut dysbiosis and hypertension needs further exploration.
Review
Pharmacology & Pharmacy
Dan-na Huang, Fang-fang Wu, Ai-hua Zhang, Hui Sun, Xi-jun Wang
Summary: The study recaps the potential therapeutic effects and mechanisms of action of berberine in treating rheumatoid arthritis, as well as discusses its modulation of gut microbiota during RA. Berberine may be a promising lead drug with multi-functional activities for the therapeutic strategy of RA.
PHARMACOLOGICAL RESEARCH
(2021)
Article
Chemistry, Analytical
Taiping Li, Mengli Zhang, Zhien Tan, Jianhua Miao, Yanmei He, Aihua Zhang, Min Ou, Danna Huang, Fangfang Wu, Xijun Wang
Summary: A rapid and robust method was established for the comprehensive analysis of the chemical composition of Jigucao capsule for the first time in this study, providing important reference for the quality control and pharmacodynamic material basis research of Jigucao capsule.
JOURNAL OF SEPARATION SCIENCE
(2022)
Article
Pharmacology & Pharmacy
Hui-Yu Li, Hui Sun, Ai-Hua Zhang, Lu-Wen He, Shi Qiu, Jun-Ru Xue, Fangfang Wu, Xi-Jun Wang
Summary: Through urine metabolomics analysis, SMYAT was found to have significant antithrombotic and anti-inflammatory effects on thromboangiitis obliterans. It can regulate the metabolic profile of TAO rats, and the therapeutic effect is closely related to tyrosine metabolism, vitamin B6 metabolism, and cysteine and methionine metabolism.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Pharmacology & Pharmacy
Yanmei He, Mengli Zhang, Taiping Li, Zhien Tan, Aihua Zhang, Min Ou, Danna Huang, Fangfang Wu, Xijun Wang
Summary: This study investigated the mechanism of Jigucao capsule (JGCC) in the treatment of Dampness-heat Jaundice Syndrome (DHJS) using urine metabolomics. The results showed that JGCC significantly improved clinical biochemical indexes and histopathological changes, and adjusted disturbed biomarkers, indicating its remarkable effect on the treatment of DHJS.
FRONTIERS IN PHARMACOLOGY
(2022)
Review
Oncology
Jing Li, Ai-hua Zhang, Fang-fang Wu, Xi-jun Wang
Summary: Dysbiosis of gut bacteria is associated with colorectal cancer (CRC), and the association analysis of metabolomics and gut microbiome will provide novel strategies for the prevention, diagnosis, and therapy of CRC.
FRONTIERS IN ONCOLOGY
(2022)
Article
Microbiology
Changhong Ding, Dan Yu, Yiyang Chen, Junyi Zhu, Junkai Wu, Xiaowei Du, Xijun Wang
Summary: This study sequenced and analyzed the genome of endophytic fungus Fusarium sp. C39 and found key enzyme genes involved in saponin synthesis. The proposed mechanism of saponin production in Fusarium sp. C39 provides a theoretical basis for efficient and low-cost production of saponins.
JOURNAL OF BASIC MICROBIOLOGY
(2022)
Article
Biochemical Research Methods
Taiping Li, Yanmei He, Mengli Zhang, Zhien Tan, Aihua Zhang, Min Ou, Danna Huang, Fangfang Wu, Xijun Wang
Summary: This study explored the potential mechanism of the Chinese patent medicine Jigucao capsule in treating the serum metabolic profile of rats with Yanghuang syndrome. The results showed that Jigucao capsule reduced inflammatory cells, inhibited the proliferation of bile duct epithelial cells and hepatocyte necrosis. It also significantly reversed abnormal levels of certain metabolites related to lipid and carbohydrate metabolism. In conclusion, Jigucao capsule has a therapeutic effect on rats with Yanghuang syndrome, and this study provides basic data for further pharmacodynamic studies.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Xing-Hua Li, Feng-Ting Yin, Xiao-Hang Zhou, Ai-Hua Zhang, Hui Sun, Guang-Li Yan, Xi-Jun Wang
Summary: This study summarizes the key pathways and targets of natural compounds from traditional Chinese medicine in the treatment of ischemic stroke by reviewing relevant literature over the past five years. This provides a valuable information base for the future development of effective treatments for ischemic stroke.
Article
Biochemistry & Molecular Biology
Qingyu Zhang, Aihua Zhang, Fangfang Wu, Xijun Wang
Summary: This study used metabonomics technology to reveal the regulatory effect of Yunnan Baiyao on small molecular metabolites in promoting blood circulation and removing blood stasis, and identified 28 urine biomarkers. The results demonstrated that Yunnan Baiyao improved the pathological indexes in rats with blood stasis syndrome and clarified the relevant metabolic pathways involved in its treatment.
Article
Chemistry, Analytical
Nannan Huang, Dan Yu, Jinhai Huo, Junkai Wu, Yiyang Chen, Xiaowei Du, Xijun Wang
Summary: This study conducted solid fermentation of Dioscorea nipponica using endophytic fungi C39 and found that it could effectively transform the saponin components, leading to a significant increase in diosgenin concentration. Furthermore, 11 new metabolites were identified during the biotransformation process of D. nipponica, and the transformation pathways were inferred based on the variation trend of saponins.
JOURNAL OF ANALYTICAL METHODS IN CHEMISTRY
(2022)
Article
Pharmacology & Pharmacy
Wen-feng Wei, Hui Sun, Shao-bo Liu, Sheng-wen Lu, Ai-hua Zhang, Wan-ying Wang, Wen-jun Chai, Fang-fang Wu, Guang-li Yan, Yu Guan, Xi-jun Wang
Summary: By studying animal models and utilizing metabonomics tools, the effective components of ZhiziBaipi Decoction in treating Damp-heat jaundice syndrome and their correlation with biomarkers were identified, providing new insights into its therapeutic mechanism.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Pharmacology & Pharmacy
Taiping Li, Fangfang Wu, Aihua Zhang, Hui Dong, Ihsan Ullah, Hao Lin, Jianhua Miao, Hui Sun, Ying Han, Yanmei He, Xijun Wang
Summary: By combining untargeted metabolomics and serum pharmacochemistry of traditional Chinese medicine, this study revealed the metabolic mechanisms and key pharmacodynamic components of Wutou decoction in rheumatoid arthritis rats, providing a theoretical basis for quality control investigation.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Pharmacology & Pharmacy
Ling Kong, Ye Sun, Hui Sun, Ai-hua Zhang, Bo Zhang, Nan Ge, Xi-jun Wang
Summary: This study applied the chinmedomics strategy to discover the effective constituents of KLX in the treatment of diabetic retinopathy (DR), and revealed some of the mechanisms involved.
FRONTIERS IN PHARMACOLOGY
(2022)
Review
Endocrinology & Metabolism
Ye Sun, Ling Kong, Ai-Hua Zhang, Ying Han, Hui Sun, Guang-Li Yan, Xi-Jun Wang
Summary: Diabetic retinopathy, a serious complication of diabetes, is a leading cause of blindness worldwide. The irreversibility of the condition makes current treatment methods unsatisfactory. Early detection and intervention are important in reducing the damage caused by diabetic retinopathy. Metabolomics, a branch of systems biology, can identify characteristic metabolic changes associated with the progression of diabetic retinopathy and discover potential biomarkers for clinical diagnosis and treatment. This review summarizes the known metabolomics biomarkers of diabetic retinopathy and discusses the possibility of the arginine-creatine metabolic pathway as a therapeutic strategy.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Plant Sciences
Feng-ting Yin, Xiao-hang Zhou, Shu-yu Kang, Xing-hua Li, Jing Li, Ihsan Ullah, Ai-hua Zhang, Hui Sun, Xi-jun Wang
Summary: This study explored the main constituents and potential targets of Dachengqi Decoction (DCQ) in the treatment of colorectal cancer (CRC) using serum pharmacochemistry and network pharmacology. The results showed that flavonoids and anthraquinones were the main active substances of DCQ, which may exert anti-tumor effects by regulating targets such as MAPK, IL-6, and VEGF, as well as pathways including PI3K-AKT signaling pathway, AGE-RAGE signaling pathway, and IL-17 signaling pathway.
JOURNAL OF ETHNOPHARMACOLOGY
(2022)
Article
Medical Laboratory Technology
Jiansheng Lin, Weihua Lin, Yiming Lin, Weilin Peng, Zhenzhu Zheng
Summary: This study retrospectively analyzed the cases of Chinese infants with NICCD and identified multiple genetic mutations. The study also found comorbidity of NICCD and other inborn errors of metabolism in some patients.
CLINICA CHIMICA ACTA
(2024)
Review
Medical Laboratory Technology
Lihua Guan, Wei Su, Jian Zhong, Ling Qiu
Summary: Multiple myeloma is characterized by excessive production of monoclonal immunoglobulins. Routine screening methods are insufficient for detecting low levels of M proteins, but advances in mass spectrometry enable reliable detection of low abundance serum biomarkers for minimal residual disease assessment in multiple myeloma.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Liya Zhu, Chao Zhu, Jialie Jin, Jinxin Wang, Xiaojing Zhao, Rongxi Yang
Summary: This study found an association between blood-based ITGB2 methylation and coronary heart disease (CHD), with hypomethylation of ITGB2 being a risk factor for CHD. Additionally, the combination of ITGB2 methylation and conventional CHD risk factors could efficiently discriminate CHD patients from controls.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
H. Al Habobe, E. B. Haverkort, K. Nazmi, A. P. Van Splunter, R. H. H. Pieters, F. J. Bikker
Summary: Saliva diagnostics have become popular due to their non-invasive nature and patient-friendly collection process. However, the choice of saliva collection method can affect the measured levels of various biomarkers.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Abdurrahman Coskun, Anna Carobene, Ozlem Demirelce, Michele Mussap, Federica Braga, Ebru Sezer, Aasne Karine Aarsand, Sverre Sandberg, Pilar Fernandez Calle, Jorge Diaz-Garzon, Metincan Erkaya, Cihan Coskun, Esila Nur Erol, Hunkar Dag, Bill Bartlett, Mustafa Serteser, Niels Jonker, Ibrahim Unsal
Summary: In this study, BV estimates for 22 AAs were provided based on a large sample size, and it was found that there are differences in CVI estimates between males and females for most AAs, which has implications for the clinical interpretation and use of AAs.
CLINICA CHIMICA ACTA
(2024)
Review
Medical Laboratory Technology
Valentinus Besin, Farizky Martriano Humardani, Trilis Yulianti, Matthew Justyn
Summary: The study of Parkinson's Disease in Asian populations has revealed the impact of genetic variants on multiple biological pathways and highlighted shared genetic susceptibility with other diseases. These findings emphasize the importance of personalized treatment based on individual genetic profiles.
CLINICA CHIMICA ACTA
(2024)
Review
Medical Laboratory Technology
Simona Ferraro, Sara Benedetti, Savina Mannarino, Santica Marcovina, Elia Mario Biganzoli, Gianvincenzo Zuccotti
Summary: Risk stratification for cardio-vascular disease should be implemented in childhood to promote early prevention strategies, as atherosclerotic lesions can be present even in very young individuals. Evaluating pediatric CV risk factors/clinical conditions and conducting lipid profile and genetic testing can help identify children at risk of future CV events and guide appropriate therapeutic options.
CLINICA CHIMICA ACTA
(2024)
Review
Medical Laboratory Technology
Ahmad Mobed, Bita Abdi, Sajjad Masoumi, Mohammad Mikaeili, Elham Shaterian, Hamed Shaterian, Esmat Sadat Kazemi, Mahdiye Shirafkan
Summary: Reproductive biomarkers play important regulatory roles in women. The discovery and quantification of these biomarkers are clinically significant. Various detection strategies, including nanotechnology-based methods, have been developed. This article provides an in-depth introduction to the latest advances in biosensor and nanosensor research for detecting and quantitatively identifying reproductive biomarkers.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Chengfang Tang, Fang Tang, Yanna Cai, Minyi Tan, Sichi Liu, Ting Xie, Xiang Jiang, Yonglan Huang
Summary: This study proposes an effective method for screening X-ALD and evaluates the performance of newborn screening for X-ALD in Guangzhou. The LC-MS/MS method can accurately identify X-ALD through analysis of C26:0-LPC.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Lan Liu, Jiamin Wang, Xijing Liu, Jing Wang, Lin Chen, Hongmei Zhu, Jingqun Mai, Ting Hu, Shanling Liu
Summary: The 16p11.2 deletion is a common genetic cause of neurodevelopmental disorders, with prenatal and postnatal presentations including vertebral malformations and language impairment. The majority of deletions are de novo and may be associated with MAPK3 and histidine-associated metabolism.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Sneha Venkatesalu, Shanmugapriya Dilliyappan, Avanthika Satish Kumar, Thirunavukkarasu Palaniyandi, Gomathy Baskar, Maddaly Ravi, Asha Sivaji
Summary: Microfluidics is a science and technology that deals with less sample-to-more precision in vitro analysis. It has wide applications in cancer theranostics, enabling precise diagnosis and personalized treatment.
CLINICA CHIMICA ACTA
(2024)
Review
Medical Laboratory Technology
Yuanqin Zhao, Wei Deng, Zhaoyue Wang, Yanxia Wang, Hongyu Zheng, Kun Zhou, Qian Xu, Le Bai, Huiting Liu, Zhong Ren, Zhisheng Jiang
Summary: The cardiovascular system and the central nervous system exhibit a coordinated developmental process during embryonic development. Congenital heart disease (CHD) is the most common congenital disorder, and neurodevelopmental disorders (NDD) are common complications in CHD patients. Both genetic and non-genetic factors contribute to the co-occurrence of CHD and NDD. Further research should focus on identifying common molecular mechanisms underlying this co-occurrence and promoting the research and treatment of developmental disorders related to the cardiovascular and central nervous systems.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Karol Gostomczyk, Ewelina Lukaszewska, Jedrzej Borowczak, Anita Bator, Marek Zdrenka, Magdalena Bodnar, Lukasz Szylberg
Summary: Flow cytometry improves the detection of epithelial cancer cells in peritoneal and pleural fluids compared to conventional cytology. Due to similar speciflcity and higher sensitivity, flow cytometry offers a promising alternative to cytology for patient screening.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Heping Tian, Genghuan Wang, Qi Zhong, Haihang Zhou
Summary: This study found that the decline of serum ITIH4 concentrations during the early phase after aneurysmal subarachnoid hemorrhage (aSAH) was closely related to the severity and poor prognosis of the disease. Serum ITIH4 may represent a promising prognostic biomarker of aSAH.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Xueting Zhu, Yang Yu, Jun Zhang, Yuxia Zhan, Guanghua Luo, Lu Zheng
Summary: This study successfully established a 2D-PCR method for identifying HLA-B*15:02 through a two-tube reaction. This method can distinguish HLA-B*15:02 from 16 highly homologous HLA-B*15 alleles. Among 1830 samples from the clinical general population, 3 HLA-B*15:02 homozygotes and 84 HLA-B*15:02 heterozygotes were detected.
CLINICA CHIMICA ACTA
(2024)
