Article
Biochemistry & Molecular Biology
Layth Ben-Trad, Constantin Ionut Matei, Mirela Maria Sava, Samira Filali, Marie-Eve Duclos, Yves Berthier, Michel Guichardant, Nathalie Bernoud-Hubac, Ofelia Maniti, Ahmed Landoulsi, Marie-Genevieve Blanchin, Pierre Miossec, Thierry Granjon, Ana-Maria Trunfio-Sfarghiu
Summary: This study compares the biochemical, tribological, and ultrastructural properties of synovial fluid in healthy individuals and patients with osteoarthritis and arthritis, finding differences that indicate the importance of synovial fluid in joint mechanics.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Chiara Baggio, Roberto Luisetto, Carlotta Boscaro, Anna Scanu, Roberta Ramonda, Mattia Albiero, Paolo Sfriso, Francesca Oliviero
Summary: Genomic damage is associated with autoimmune processes, chronic inflammation, and apoptosis. The T cell compartment may exhibit overall genomic instability in certain rheumatological diseases. However, there is limited knowledge regarding leukocyte abnormalities in synovial fluid (SF) and their connection to inflammation. This study aimed to investigate cellular phenotypes in SF from patients with various inflammatory arthropathies. The results showed that inflammatory SF is associated with genomic instability and abnormal cell subsets.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Orthopedics
Anne-Mari Mustonen, Janne Capra, Kirsi Rilla, Petri Lehenkari, Sanna Oikari, Tommi Kaariainen, Antti Joukainen, Heikki Kroger, Tommi Paakkonen, Johanna Matilainen, Petteri Nieminen
Summary: The study successfully developed a method for quantifying EV, HA particles, and HA-EV in human knee joint SF using CLSM and image analysis. The results showed that the fluorescence intensities of EV and HA particles were lower in RA patients compared to the control group and OA group, indicating potential implications for diagnosis and therapy in rheumatoid arthritis SF.
BMC MUSCULOSKELETAL DISORDERS
(2021)
Letter
Immunology
Francesco Caso, Anella Saviano, Marco Tasso, Federica Raucci, Noemi Marigliano, Saverio Passavanti, Paola Frallonardo, Roberta Ramonda, Vincenzo Brancaleone, Mariarosaria Bucci, Raffaele Scarpa, Luisa Costa, Francesco Maione
Summary: This study compared the expression levels of cyto-chemokines in PsA and RA synovial fluid, finding unique and specific modulation of CCL-2, G-CSF, IL-1 beta, and TNF-alpha in RA synovial fluid, and significantly higher levels of ICAM-1, IL-2, IL-6, IL-17A, C5a, and CXCL-9/12 in PsA patients. The expression of CCR2 was significantly upregulated in both PsA and RA groups, along with specific Th and Treg transcription factors such as STAT3/4 and FOXP3.
AUTOIMMUNITY REVIEWS
(2022)
Article
Medicine, General & Internal
Andrea Cuervo, Raquel Celis, Antonio Julia, Alicia Usategui, Regina Fare, Julio Ramirez, Ana Belen Azuaga, Andres Lorenzo, Raimon Sanmarti, Jose L. Pablos, Juan D. Canete
Summary: The study found that the cellular composition of synovium in undifferentiated arthritis (UA) patients differs from rheumatoid arthritis (RA) and psoriatic arthritis (PsA), with higher cellular infiltrate density in the UA group. Initial expression of the interferon inducible gene MxA may serve as a biomarker for progression to RA, while higher mast cell and fibroblastic density may be associated with PsA progression.
FRONTIERS IN MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Rebecca Sohn, Marius Junker, Andrea Meurer, Frank Zaucke, Rainer H. Straub, Zsuzsa Jenei-Lanzl
Summary: The study showed that synoviocytes of OA and RA patients have the ability to synthesize adenosine/inosine, with adenosine predominantly responsible for the anti-inflammatory effects. Inhibiting CD73 increased IL-6 and decreased IL-10, while inhibiting ADA reduced IL-6 and induced IL-10. A(2A)- and A(2B)AR play a crucial role in mediating anti-inflammatory or pro-inflammatory effects.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Rheumatology
Mingyue Cheng, Yan Zhao, Yazhou Cui, Chaofang Zhong, Yuguo Zha, Shufeng Li, Guangxiang Cao, Mian Li, Lei Zhang, Kang Ning, Jinxiang Han
Summary: This study investigated the role of microbial dysbiosis and metabolic disorders in different stages of rheumatoid arthritis (RA). The findings suggest that microbial dysbiosis and metabolic disorders exhibit distinct patterns and play stage-specific roles in RA. This provides new insights for prognosis and therapy of RA.
ANNALS OF THE RHEUMATIC DISEASES
(2022)
Article
Rheumatology
Nicole Yager, Suzanne Cole, Alicia Lledo Lara, Ash Maroof, Frank Penkava, Julian C. Knight, Paul Bowness, Hussein Al-Mossawi
Summary: By using mass cytometry combined with transcriptomic analysis, this study identified significant differences in myeloid cell populations between psoriatic arthritis (PsA) synovial fluid (SF) and blood, with expansion of intermediate monocytes, macrophages, and dendritic cells in SF. Myeloid cells, particularly classical monocytes, intermediate monocytes, and macrophages, were found to produce proinflammatory mediators like osteopontin and CCL2 spontaneously in PsA SF, while minimal cytokine production was detected in T cells. Gene expression analysis revealed upregulation of osteopontin and CCL2 genes in PsA monocytes/macrophages, which were also elevated at the protein level in PsA SF.
ANNALS OF THE RHEUMATIC DISEASES
(2021)
Article
Cell Biology
Petteri Nieminen, Wilhelmiina Hamalainen, Juha Savinainen, Marko Lehtonen, Saara Lehtiniemi, Juho Rinta-Paavola, Petri Lehenkari, Tommi Kaariainen, Antti Joukainen, Heikki Kroger, Tommi Paakkonen, Anne-Mari Mustonen
Summary: This study characterized the metabolic profiles of synovial fluid and infrapatellar fat pad in patients with osteoarthritis (OA) and rheumatoid arthritis (RA). Significant differences in metabolite profiles were found between OA and RA, and alterations in metabolic pathways related to disease progression were identified. These findings provide insights into the pathogeneses of OA and RA and have potential implications for the development of biomarkers for early diagnosis and therapeutic targets.
Article
Rheumatology
Li Zheng, Fanlei Hu, Wenjie Bian, Yingni Li, Linqi Zhang, Lianjie Shi, Xiaoxu Ma, Yanying Liu, Xuewu Zhang, Zhanguo Li
Summary: Dkk-1 exacerbates inflammation, cartilage erosion, and angiogenesis mediated by synovial fibroblasts in RA. Modulation of DKK-1 expression may facilitate the development of novel strategies to control RA.
CLINICAL RHEUMATOLOGY
(2021)
Article
Rheumatology
Irina Gessl, Mihaela Popescu, Victoria Schimpl, Gabriela Supp, Thomas Deimel, Martina Durechova, Miriam Hucke, Michaela Loiskandl, Paul Studenic, Michael Zauner, Josef S. Smolen, Daniel Aletaha, Peter Mandl
Summary: Clinical tenderness may not always indicate active inflammation in RA, PsA, and OA. While tenderness in early arthritis may be more associated with inflammation, established disease is better explained by joint damage and malalignment.
ANNALS OF THE RHEUMATIC DISEASES
(2021)
Article
Immunology
Viviana Marzaioli, Mary Canavan, Achilleas Floudas, Keelin Flynn, Ronan Mullan, Douglas J. Veale, Ursula Fearon
Summary: Dendritic cells (DC) play a crucial role in the development of inflammatory arthritis. A unique population of DC derived from monocytes, called CD209/CD14(+) DC, was identified in the blood of healthy individuals as well as patients with rheumatoid arthritis (RA) and psoriatic arthritis (PsA). These CD209/CD14(+) DC exhibited distinct characteristics and increased expression of inflammatory cytokines in the circulation of patients. Furthermore, they displayed a unique inflammatory and maturation phenotype in the inflamed joint. A novel protocol for isolating and expanding CD209(+) DC was developed, revealing transcriptional differences between RA and PsA CD209(+) DC. Additionally, culture of healthy CD209(+) DC with synovial fluid from patients with IA induced the development of CD209/CD14(+) DC, accompanied by changes in chemokine receptor and chemokine expression. The JAK/STAT pathway was found to regulate the function of CD209/CD14(+) DC, suggesting its potential as a therapeutic target for reducing inflammation. Overall, this study provides insights into the role of CD209/CD14(+) DC in IA and highlights the potential of targeting the JAK/STAT pathway for therapeutic intervention.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Cell Biology
Clara Cambria, Francesca Ingegnoli, Eleonora Borzi, Laura Cantone, Lavinia Agra Coletto, Alessandra Stefania Rizzuto, Orazio De Lucia, Sabrina Briguglio, Massimiliano Ruscica, Roberto Caporali, Valentina Bollati, Massimiliano Buoli, Flavia Antonucci
Summary: Arthritides is a group of diverse disorders that affect joints, including localized joint disorders and systemic autoimmune-driven diseases. Poor mental health is common among arthritides patients. Research has shown that extracellular vesicles derived from synovial fluid can affect neuronal function, and the concentration of synovial vesicles is associated with psychiatric symptoms in arthritides patients.
Article
Multidisciplinary Sciences
Bin Zhao, Lingting Zeng, Danyang Chen, Songqing Xie, Zhaokui Jin, Guanglin Li, Wei Tang, Qianjun He
Summary: This study proposes a concept of photocatalytic regulation of the synovial microenvironment (SME) for arthritis treatment. Monodispersive hydrogen-doped titanium dioxide nanorods with a rutile single-crystal structure are developed to achieve near infrared-photocatalytic generation of hydrogen molecules and depletion of lactic acid (LA) in a collagen-induced mouse model of rheumatoid arthritis. The results show that locally produced hydrogen molecules scavenge reactive oxygen species and regulate the synovial microenvironment, thereby preventing synovial pannus formation and cartilage destruction.
Article
Immunology
Nuria Barbarroja, Maria Dolores Lopez-Montilla, Laura Cuesta-Lopez, Carlos Perez-Sanchez, Miriam Ruiz-Ponce, Clementina Lopez-Medina, Maria Lourdes Ladehesa-Pineda, Chary Lopez-Pedrera, Alejandro Escudero-Contreras, Eduardo Collantes-Estevez, Ivan Arias-de la Rosa
Summary: This study characterized the inflammatory proteome of synovial fluid in patients with Psoriatic Arthritis (PsA) using a high-throughput proteomic platform. The results showed the presence of 79 inflammation-related proteins in the synovial fluid of PsA patients, and identified two specific phenotypes based on the levels of these proteins. Additionally, compared to patients with osteoarthritis, PsA patients had significantly altered levels of 39 proteins specifically related to cell migration and inflammatory response.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Anna Scanu, Roberto Luisetto, Francesca Oliviero, Francesca Galuppini, Vanni Lazzarin, Gianmaria Pennelli, Stefano Masiero, Leonardo Punzi
Summary: The study showed that BPI alone or in combination with HA can improve inflammation in animal models of CIA and crystal-induced inflammation. The combined use of BPI and HA resulted in greater improvement in both models compared to the administration of the substances alone.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Dentistry, Oral Surgery & Medicine
Stefano Sivolella, Anna Scanu, Zijing Xie, Sara Vianello, Edoardo Stellini
Summary: Biobanks are non-profit services that collect, process, store and distribute biological samples and data for research and diagnostic purposes. In dentistry, biobanks can be used for studying oral and systemic diseases, such as oral cancer, dental caries and facial pain.
JAPANESE DENTAL SCIENCE REVIEW
(2022)
Article
Multidisciplinary Sciences
Francesca Oliviero, Anna Scanu, Paola Galozzi, Roberta Ramonda
Summary: Synovial fluid analysis is crucial in diagnosing osteoarthritis, as it helps determine the non-inflammatory nature of the fluid and identifies the presence of calcium crystals.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2022)
Article
Medicine, Research & Experimental
Oliviero Francesca, Galozzi Paola, Padoan Andrea, Lorenzin Mariagrazia, Scanu Anna, Ramonda Roberta
Summary: This study investigated the frequency and disease distribution of cytophagocytic mononuclear (CPM) cells in synovial fluid of patients with rheumatic diseases over a 12-year period. The results showed a strong association between CPM cells and seronegative spondyloarthritis (SpA), with a seasonal variation in their incidence, peaking in spring and autumn. This suggests that the presence of CPM cells in synovial fluid may offer diagnostic insights into SpA.
EXPERIMENTAL BIOLOGY AND MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Chiara Baggio, Roberto Luisetto, Carlotta Boscaro, Anna Scanu, Roberta Ramonda, Mattia Albiero, Paolo Sfriso, Francesca Oliviero
Summary: Genomic damage is associated with autoimmune processes, chronic inflammation, and apoptosis. The T cell compartment may exhibit overall genomic instability in certain rheumatological diseases. However, there is limited knowledge regarding leukocyte abnormalities in synovial fluid (SF) and their connection to inflammation. This study aimed to investigate cellular phenotypes in SF from patients with various inflammatory arthropathies. The results showed that inflammatory SF is associated with genomic instability and abnormal cell subsets.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Marta Favero, Francesca Ometto, Elisa Belluzzi, Giacomo Cozzi, Laura Scagnellato, Francesca Oliviero, Pietro Ruggieri, Andrea Doria, Mariagrazia Lorenzin, Roberta Ramonda
Summary: This study aimed to evaluate the association between fetuin-A levels and radiographic sacroiliitis and syndesmophytes in early axSpA patients, and to identify potential predictors of radiographic damage. Results showed that fetuin-A levels were negatively associated with sacroiliac joint damage and may serve as a biomarker for predicting early structural damage.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Editorial Material
Pharmacology & Pharmacy
Anna Lisa Giuliani, Alessandra Bortoluzzi, Francesca Oliviero
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Oncology
Aamir Amin, Marzia Morello, Maria Raffaella Petrara, Beatrice Rizzo, Francesco Argenton, Anita De Rossi, Silvia Giunco
Summary: Blocking telomerase (TERT) to erode telomeres and inhibit cancer cell proliferation seems impractical. However, evidence suggests that TERT has telomere-length independent functions that promote tumor growth. Understanding the mechanisms behind these effects is crucial in establishing TERT as a therapeutic target. By inhibiting TERT in B-cell lymphoproliferative disorders and malignancies, we found that it impairs the NF-kappa B p65 pathway, resulting in decreased MYC expression and cell cycle arrest. In vivo results in zebrafish models support these findings, indicating the potential of combining TERT inhibition with chemotherapy for effective cancer treatment.
Review
Biochemistry & Molecular Biology
Chiara Baggio, Sara Bindoli, Irina Guidea, Andrea Doria, Francesca Oliviero, Paolo Sfriso
Summary: IL-18 is a potent pro-inflammatory cytokine involved in various immune processes. It plays a key role in autoinflammatory diseases and may be a useful diagnostic and monitoring tool. Researchers are investigating IL-18 as a therapeutic target for inflammatory diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Chemistry, Multidisciplinary
Maria Chiara Maccarone, Anna Scanu, Gianluca Regazzo, Antonella Fioravanti, Stefano Masiero
Summary: This review explores the potential benefits of balneotherapy (BT) as a complementary intervention for managing hand osteoarthritis (HOA), based on recent randomized controlled trials (RCTs). The findings indicate that BT, particularly when using specific methods such as mud packs and mineral-rich baths, shows promise in reducing pain and improving functionality and quality of life, especially in the initial stages of the condition. Further research and larger-scale trials are needed to validate these preliminary findings.
APPLIED SCIENCES-BASEL
(2023)
Review
Medicine, Research & Experimental
Paola Galozzi, Sara Bindoli, Andrea Doria, Paolo Sfriso
Summary: Adult-onset Still's disease is a rare autoinflammatory disorder characterized by excessive cytokine release and joint and systemic symptoms. Blockade of IL-1 and IL-6 has been effective in controlling and achieving remission in AOSD patients.
BIOLOGICS-TARGETS & THERAPY
(2022)
Article
Medicine, General & Internal
Lucrezia Tognolo, Maria Chiara Maccarone, Stefania De Trane, Anna Scanu, Stefano Masiero, Pietro Fiore
Summary: Therapeutic exercise, intra-articular viscosupplementation with Hyaluronic Acid, and Platelet Rich Plasma treatment have been found to have beneficial effects on preventing cartilage degeneration, reducing pain, and enhancing physical function in the conservative management of early knee osteoarthritis. A multimodal treatment combining therapeutic exercise and moderate aerobic activity is indicated to prevent kOA development, while minimally invasive injection therapy can contribute to improving motor function and symptomatology in symptomatic cases.
MEDICINA-LITHUANIA
(2022)
Article
Medical Laboratory Technology
Jiansheng Lin, Weihua Lin, Yiming Lin, Weilin Peng, Zhenzhu Zheng
Summary: This study retrospectively analyzed the cases of Chinese infants with NICCD and identified multiple genetic mutations. The study also found comorbidity of NICCD and other inborn errors of metabolism in some patients.
CLINICA CHIMICA ACTA
(2024)
Review
Medical Laboratory Technology
Lihua Guan, Wei Su, Jian Zhong, Ling Qiu
Summary: Multiple myeloma is characterized by excessive production of monoclonal immunoglobulins. Routine screening methods are insufficient for detecting low levels of M proteins, but advances in mass spectrometry enable reliable detection of low abundance serum biomarkers for minimal residual disease assessment in multiple myeloma.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Liya Zhu, Chao Zhu, Jialie Jin, Jinxin Wang, Xiaojing Zhao, Rongxi Yang
Summary: This study found an association between blood-based ITGB2 methylation and coronary heart disease (CHD), with hypomethylation of ITGB2 being a risk factor for CHD. Additionally, the combination of ITGB2 methylation and conventional CHD risk factors could efficiently discriminate CHD patients from controls.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
H. Al Habobe, E. B. Haverkort, K. Nazmi, A. P. Van Splunter, R. H. H. Pieters, F. J. Bikker
Summary: Saliva diagnostics have become popular due to their non-invasive nature and patient-friendly collection process. However, the choice of saliva collection method can affect the measured levels of various biomarkers.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Abdurrahman Coskun, Anna Carobene, Ozlem Demirelce, Michele Mussap, Federica Braga, Ebru Sezer, Aasne Karine Aarsand, Sverre Sandberg, Pilar Fernandez Calle, Jorge Diaz-Garzon, Metincan Erkaya, Cihan Coskun, Esila Nur Erol, Hunkar Dag, Bill Bartlett, Mustafa Serteser, Niels Jonker, Ibrahim Unsal
Summary: In this study, BV estimates for 22 AAs were provided based on a large sample size, and it was found that there are differences in CVI estimates between males and females for most AAs, which has implications for the clinical interpretation and use of AAs.
CLINICA CHIMICA ACTA
(2024)
Review
Medical Laboratory Technology
Valentinus Besin, Farizky Martriano Humardani, Trilis Yulianti, Matthew Justyn
Summary: The study of Parkinson's Disease in Asian populations has revealed the impact of genetic variants on multiple biological pathways and highlighted shared genetic susceptibility with other diseases. These findings emphasize the importance of personalized treatment based on individual genetic profiles.
CLINICA CHIMICA ACTA
(2024)
Review
Medical Laboratory Technology
Simona Ferraro, Sara Benedetti, Savina Mannarino, Santica Marcovina, Elia Mario Biganzoli, Gianvincenzo Zuccotti
Summary: Risk stratification for cardio-vascular disease should be implemented in childhood to promote early prevention strategies, as atherosclerotic lesions can be present even in very young individuals. Evaluating pediatric CV risk factors/clinical conditions and conducting lipid profile and genetic testing can help identify children at risk of future CV events and guide appropriate therapeutic options.
CLINICA CHIMICA ACTA
(2024)
Review
Medical Laboratory Technology
Ahmad Mobed, Bita Abdi, Sajjad Masoumi, Mohammad Mikaeili, Elham Shaterian, Hamed Shaterian, Esmat Sadat Kazemi, Mahdiye Shirafkan
Summary: Reproductive biomarkers play important regulatory roles in women. The discovery and quantification of these biomarkers are clinically significant. Various detection strategies, including nanotechnology-based methods, have been developed. This article provides an in-depth introduction to the latest advances in biosensor and nanosensor research for detecting and quantitatively identifying reproductive biomarkers.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Chengfang Tang, Fang Tang, Yanna Cai, Minyi Tan, Sichi Liu, Ting Xie, Xiang Jiang, Yonglan Huang
Summary: This study proposes an effective method for screening X-ALD and evaluates the performance of newborn screening for X-ALD in Guangzhou. The LC-MS/MS method can accurately identify X-ALD through analysis of C26:0-LPC.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Lan Liu, Jiamin Wang, Xijing Liu, Jing Wang, Lin Chen, Hongmei Zhu, Jingqun Mai, Ting Hu, Shanling Liu
Summary: The 16p11.2 deletion is a common genetic cause of neurodevelopmental disorders, with prenatal and postnatal presentations including vertebral malformations and language impairment. The majority of deletions are de novo and may be associated with MAPK3 and histidine-associated metabolism.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Sneha Venkatesalu, Shanmugapriya Dilliyappan, Avanthika Satish Kumar, Thirunavukkarasu Palaniyandi, Gomathy Baskar, Maddaly Ravi, Asha Sivaji
Summary: Microfluidics is a science and technology that deals with less sample-to-more precision in vitro analysis. It has wide applications in cancer theranostics, enabling precise diagnosis and personalized treatment.
CLINICA CHIMICA ACTA
(2024)
Review
Medical Laboratory Technology
Yuanqin Zhao, Wei Deng, Zhaoyue Wang, Yanxia Wang, Hongyu Zheng, Kun Zhou, Qian Xu, Le Bai, Huiting Liu, Zhong Ren, Zhisheng Jiang
Summary: The cardiovascular system and the central nervous system exhibit a coordinated developmental process during embryonic development. Congenital heart disease (CHD) is the most common congenital disorder, and neurodevelopmental disorders (NDD) are common complications in CHD patients. Both genetic and non-genetic factors contribute to the co-occurrence of CHD and NDD. Further research should focus on identifying common molecular mechanisms underlying this co-occurrence and promoting the research and treatment of developmental disorders related to the cardiovascular and central nervous systems.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Karol Gostomczyk, Ewelina Lukaszewska, Jedrzej Borowczak, Anita Bator, Marek Zdrenka, Magdalena Bodnar, Lukasz Szylberg
Summary: Flow cytometry improves the detection of epithelial cancer cells in peritoneal and pleural fluids compared to conventional cytology. Due to similar speciflcity and higher sensitivity, flow cytometry offers a promising alternative to cytology for patient screening.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Heping Tian, Genghuan Wang, Qi Zhong, Haihang Zhou
Summary: This study found that the decline of serum ITIH4 concentrations during the early phase after aneurysmal subarachnoid hemorrhage (aSAH) was closely related to the severity and poor prognosis of the disease. Serum ITIH4 may represent a promising prognostic biomarker of aSAH.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Xueting Zhu, Yang Yu, Jun Zhang, Yuxia Zhan, Guanghua Luo, Lu Zheng
Summary: This study successfully established a 2D-PCR method for identifying HLA-B*15:02 through a two-tube reaction. This method can distinguish HLA-B*15:02 from 16 highly homologous HLA-B*15 alleles. Among 1830 samples from the clinical general population, 3 HLA-B*15:02 homozygotes and 84 HLA-B*15:02 heterozygotes were detected.
CLINICA CHIMICA ACTA
(2024)