Article
Biochemistry & Molecular Biology
Parisa Fayyazpour, Effat Alizadeh, Vahid Hosseini, Ashkan Kalantary-Charvadeh, Mitra Niafar, Vahideh Sadra, Zahra Norouzi, Aysan Saebnazar, Amir Mehdizadeh, Masoud Darabi
Summary: In type 2 diabetes, dyslipidemia and increased serum free fatty acids (FFAs) negatively affect insulin secretion and worsen the disease. This study found that FFAs from diabetic serum have a detrimental effect on the stemness characteristics of adipose-derived mesenchymal stem cells, potentially impairing their regenerative capabilities.
JOURNAL OF CELLULAR BIOCHEMISTRY
(2022)
Article
Food Science & Technology
Xufang Wu, Fengen Wang, Meiqing Chen, Jiaqi Wang, Yangdong Zhang
Summary: The aim of this research was to establish a method for quantifying 10 short-chain fatty acids (FSCFAs) in raw cow milk. Acidified raw cow milk samples were processed using gas chromatography-mass spectrometry after pretreatment with hydrochloric acid ethanol (0.5%) solution. Except for iso C5:0 and anteiso C5:0 co-elution, the remaining eight FSCFAs were effectively separated by chromatography. The methodological validation showed satisfactory linear relationships, low limits of quantification, good recoveries, and precision. Our study provides a valuable method for future quality and safety control of raw milk and nutritional studies.
Article
Multidisciplinary Sciences
Hyejin Park, Woo-Young Song, Hyeonjeon Cha, Tae-Young Kim
Summary: This study developed a sample preparation protocol using mass spectrometry to minimize exogenous FFA contamination during quantification in food. The optimized method includes a combination of methanol-washed glass tube and chloroform extraction solvent to minimize errors in FFA quantification.
SCIENTIFIC REPORTS
(2021)
Article
Agriculture, Multidisciplinary
Zahir Shah, Syed Lal Badshah, Arshad Iqbal, Zamarud Shah, Abdul-Hamid Emwas, Mariusz Jaremko
Summary: Freshwater macroalgae were found to contain important secondary metabolites with medicinal properties, which can be used as biopesticides, plant growth enhancers, and remedies for diseases in farm animals. The extracts also contain essential oils that can be utilized in various industrial products.
CHEMICAL AND BIOLOGICAL TECHNOLOGIES IN AGRICULTURE
(2022)
Article
Biochemical Research Methods
Monica Mattarozzi, Nicolo Riboni, Monica Maffini, Simona Scarpella, Federica Bianchi, Maria Careri
Summary: Two commercial stationary phases, CSH C 18 and Atlantis PREMIER BEH C 18 AX, were tested for separating a mixture of 21 fatty acids. BEH C 18 AX showed superior performance due to higher surface area and anion-exchange capacity. The UHPLC-HRMS system allows fast analysis without the need for derivatization or ion-pairing reagents.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Oncology
Alicja Pakiet, Agata Jedrzejewska, Katarzyna Duzowska, Alina Waclawska, Patrycja Jablonska, Jacek Zielinski, Adriana Mika, Tomasz Sledzinski, Ewa Slominska
Summary: The aim of this study was to examine the serum fatty acids (FAs) profiles in breast cancer survivors to assess if the FA levels normalize. After treatment for breast cancer, the patients' serum FA profile differs from the profile before treatment and from controls, especially 12 months after treatment. Some changes may reflect lifestyle changes in breast cancer survivors and have an impact on the risk of recurrence.
Review
Biochemistry & Molecular Biology
Christiana Mantzourani, Maroula G. Kokotou
Summary: Fatty acids play important roles in living organisms and mass spectrometry has become a crucial technique for their analysis. Chemical derivatization is often used to improve the ionization efficiency of fatty acids. This review summarizes the different reagents used for fatty acid derivatization and discusses their applications in liquid chromatography-mass spectrometry analysis.
Article
Food Science & Technology
Christiana Mantzourani, Charikleia S. Batsika, Maroula G. Kokotou, George Kokotos
Summary: A new method was developed to simultaneously determine multiple fatty acids in yogurt samples, including both common and uncommon ones. The concentrations of various fatty acids in the samples allowed the discrimination between cow and sheep yogurt.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Multidisciplinary Sciences
Angela Criscuolo, Palina Nepachalovich, Diego Fernando Garcia-del Rio, Mike Lange, Zhixu Ni, Massimo Baroni, Gabriele Cruciani, Laura Goracci, Matthias Blueher, Maria Fedorova
Summary: This study utilizes bioinformatics and LC-MS/MS technologies to uncover the diversity and specificity of modified lipids in human blood plasma of lean and obese individuals, revealing the significant alteration of endocrine signaling by oxidized lipids in metabolic disorders.
NATURE COMMUNICATIONS
(2022)
Article
Agriculture, Multidisciplinary
Sonia Tome-Rodriguez, Carlos A. Ledesma-Escobar, Jose M. Penco-Valenzuela, Feliciano Priego-Capote
Summary: This study developed a method for qualitative and semi-quantitative determination of volatile components in thermally stressed virgin olive oils using HS-GC-MS. The volatile components were found to be closely correlated with the concentration of the main unsaturated fatty acids, explaining their impact on organoleptic properties.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2021)
Article
Biochemistry & Molecular Biology
Cheng Li, Zhiqian Liu, Carolyn Bath, Leah Marett, Jennie Pryce, Simone Rochfort
Summary: This paper presents an optimized protocol combining 3-NPH derivatization and LC-MS analysis to quantify SCFA and BHBA in bovine milk and serum. The method is sensitive, accurate, reproducible, and has a short sample preparation step. Applying this method to samples reveals significant variation in SCFA concentration between milk and serum, as well as strong correlations between some SCFAs in milk and serum samples.
Article
Chemistry, Multidisciplinary
Cody Williams, Lethiwe L. Mbuyane, Florian F. Bauer, Lucky Mokwena, Benoit Divol, Astrid Buica
Summary: The study proposes a systematic optimization procedure for the analysis of fatty acids and sterols relevant to the grape fermentation process, with reproducible results and implementation flexibility indicated for practical use in the future.
APPLIED SCIENCES-BASEL
(2021)
Article
Biochemistry & Molecular Biology
Maroula G. Kokotou, Charikleia S. Batsika, Christiana Mantzourani, George Kokotos
Summary: Recent studies have discovered the presence of oxidized saturated fatty acids in milk, which exhibit anti-cancer and anti-apoptotic effects. A liquid chromatography-high-resolution mass spectrometry method was applied to detect and quantify previously unrecognized saturated oxo fatty acids in milk. The study revealed the presence of families of oxostearic acids and oxopalmitic acids in milk, as well as the detection of ricinoleic acid in all milk samples.
Article
Multidisciplinary Sciences
Dina S. Ghallab, Eman Shawky, Reham S. Ibrahim, Mohamed M. Mohyeldin
Summary: The present study used UPLC-MS metabolite fingerprinting to characterize the metabolites of four marine algae species. It was found that some of these metabolites exhibited cytotoxic activity, providing insight into the chemistry and activity relationship of these algae species.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Analytical
Li-Xue Wang, Shi-Si Tian, Xi-Lin Song, Wen-Jie Li, Hui-Min Li, Ya-Bin Zhoa
Summary: This study explored a new method to measure the fatty acid components in fingerprint substances, improving the sensitivity and scientificity of fingerprint inspection technique. The experiment results showed that the method accurately detected the fatty acid components in fingerprint residues and revealed significant individual differences in fingerprint lipid composition.
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
(2022)
Article
Clinical Neurology
Han Soo Yoo, Seong Ho Jeong, Kyeong Taek Oh, Sangwon Lee, Young H. Sohn, Byoung Seok Ye, Mijin Yun, Phil Hyu Lee
Summary: Yoo et al. investigated the relationship between striatal dopamine depletion, brain metabolism, and cognition in patients with dementia with Lewy bodies. They found that striatal dopamine loss and brain hypometabolism are closely related, impacting cognition in a specific manner, and predicting cognitive decline. Brain hypometabolism was found to be more relevant to longitudinal cognitive outcomes than striatal dopaminergic degeneration in patients with Lewy body dementia.
Article
Geriatrics & Gerontology
Sakulrat Mankhong, Sujin Kim, Sohee Moon, Seong-Hye Choi, Hyo-Bum Kwak, Dong-Ho Park, Pratik Shah, Phil Hyu Lee, Seong Wook Yang, Ju-Hee Kang
Summary: This study discovered differentially expressed blood miRNAs in Korean AD patients and investigated their role in amyloidogenesis. However, the diagnostic utility of these miRNAs as biomarkers was limited.
JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES
(2023)
Article
Clinical Neurology
Seong Ho Jeong, Hye Sun Lee, Jin Ho Jung, Kyoungwon Baik, Young H. Sohn, Seok Jong Chung, Phil Hyu Lee
Summary: This study revealed the correlation between striatal DAT availability and the severity of WMHs, with different types of WMHs affecting cognitive function in PD patients depending on the cognitive domains.
PARKINSONISM & RELATED DISORDERS
(2022)
Article
Geriatrics & Gerontology
Ji Eun Lee, Yu Jin Shin, Yi Seul Kim, Ha Na Kim, Dong Yeol Kim, Seok Jong Chung, Han Soo Yoo, Jin Young Shin, Phil Hyu Lee
Summary: The study demonstrated that uric acid could enhance neurogenesis in Parkinson's disease models, particularly by regulating mitochondrial dynamics, promoting fusion machinery, and inhibiting fission process.
FRONTIERS IN AGING NEUROSCIENCE
(2022)
Article
Neurosciences
Young-Gun Lee, Seong Ho Jeong, Mincheol Park, Sung Woo Kang, Kyoungwon Baik, Seun Jeon, Phil Hyu Lee, Young Ho Sohn, Byoung Seok Ye
Summary: The coexistence of Alzheimer's disease pathology is common in Parkinson's disease. This study explores the implications of genetic risk scores for Alzheimer's disease (GRS-AD) in Parkinson's disease (PD). Higher GRS-AD and CSF p-tau/A beta are associated with cognitive decline in PD patients.
NPJ PARKINSONS DISEASE
(2022)
Article
Multidisciplinary Sciences
Han Soo Yoo, Eun-Chong Lee, Seok Jong Chung, Byoung Seok Ye, Young H. Sohn, Joon-Kyung Seong, Phil Hyu Lee
Summary: This study found that the occurrence of levodopa-induced dyskinesia in Parkinson's disease patients is associated with a contracted shape of the thalamus, independent of presynaptic dopamine depletion.
SCIENTIFIC REPORTS
(2022)
Article
Geriatrics & Gerontology
SeungWook Kim, Sung-Woo Kim, Young Noh, Phil Hyu Lee, Duk L. Na, Sang Won Seo, Joon-Kyung Seong
Summary: Analyzing neuroimages is an important method in the fields of neuroscience and neurology, but incompatibilities across protocols and vendors pose a major problem. In this study, we successfully corrected center effects in cortical features obtained from multicenter MRIs. Our LME model-based w-score effectively corrected the center effects while preserving disease effects, as shown in various classification tasks. This model also harmonized data that were not used for model training, making it a valuable tool in neuroimaging analysis.
FRONTIERS IN AGING NEUROSCIENCE
(2022)
Article
Neurosciences
Sooyeon Ji, Eun-Jung Choi, Beomseok Sohn, Kyoungwon Baik, Na-Young Shin, Won-Jin Moon, Seongbeom Park, Soohwa Song, Phil Hyu Lee, Dong Hoon Shin, Se-Hong Oh, Eung Yeop Kim, Jongho Lee
Summary: This study proposes a new NM-sensitive imaging method called sandwichNM, which utilizes the incidental MT effects of spatial saturation RF pulses to generate high-quality NM images using product sequences. Compared to conventional NM-sensitive imaging methods, sandwichNM provides higher NM contrast and consistent contrasts across vendors in a multi-vendor experiment.
Article
Clinical Neurology
Seong Ho Jeong, Jungho Cha, Mincheol Park, Jin Ho Jung, Byoung Seok Ye, Young H. Sohn, Seok Jong Chung, Phil Hyu Lee
Summary: This study investigated the effects of enlarged perivascular space (EPVS) on amyloid burden and cognitive function in Alzheimer's disease (AD) continuum. The results showed that BG-EPVS, CSO-EPVS, and HP-EPVS were not associated with baseline amyloid burden or cognitive function in AD continuum. However, CSO-EPVS appeared to be associated with the progression of cognitive decline independently of amyloid burden.
Article
Clinical Neurology
Seok Jong Chung, Yae Ji Kim, Yun Joong Kim, Hye Sun Lee, Seong Ho Jeong, Ji-Man Hong, Young H. Sohn, Mijin Yun, Yong Jeong, Phil Hyu Lee
Summary: Different patterns of striatal dopamine depletion are closely associated with WM structural alterations, which may contribute to heterogeneous cognitive profiles in individuals with PD.
Article
Clinical Neurology
Yae Ji Kim, Chan Wook Park, Hye Won Shin, Hye Sun Lee, Yun Joong Kim, Mijin Yun, Phil Hyu Lee, Young H. Sohn, Yong Jeong, Seok Jong Chung
Summary: This study explored the white matter structural network associated with motor reserve in patients with newly diagnosed Parkinson's disease (PD). It found that higher network strength was associated with a slower longitudinal increase in levodopa-equivalent dose (LED) during a 3-year follow-up period.
PARKINSONISM & RELATED DISORDERS
(2022)
Article
Clinical Neurology
Seong Ho Jeong, Chan Wook Park, Hye Sun Lee, Yun Joong Kim, Mijin Yun, Phil Hyu Lee, Young H. Sohn, Seok Jong Chung
Summary: The study investigated whether different patterns of striatal dopamine depletion are associated with motor deficits in Parkinson's disease. A total of 404 drug-naive patients with early-stage PD were enrolled and underwent dopamine transporter imaging. Principal component analysis identified three patterns of dopamine depletion: overall deficiency, selective loss in the sensorimotor striatum, and symmetric loss in the striatum. Linear regression analysis revealed that overall deficiency and selective loss were associated with motor deficits, while symmetric loss was not. Mediation analysis showed that dopamine deficiency in the posterior putamen mediated the association between dopamine depletion patterns and motor deficits. These findings suggest that dopamine deficiency in the posterior putamen plays a crucial role in motor deficits in PD.
JOURNAL OF NEURAL TRANSMISSION
(2023)
Review
Clinical Neurology
Jin Yong Hong, Phil Hyu Lee
Summary: Subjective cognitive complaints (SCCs), self-perceived cognitive decline, are related to objective cognitive decline. SCCs in cognitively normal individuals serve as a preclinical sign of subsequent cognitive impairment due to Alzheimer's disease. This review highlights the current research on SCCs in cognitively normal patients with Parkinson's disease, emphasizing the need for coherence in defining and assessing SCCs, and the value of SCCs in predicting cognitive decline. It also calls for further biomarker studies to establish the underlying pathological basis for these findings.
JOURNAL OF MOVEMENT DISORDERS
(2023)
Article
Neurosciences
Seong Ho Jeong, Jungho Cha, Jin Ho Jung, Mijin Yun, Young H. Sohn, Seok Jong Chung, Phil Hyu Lee
Summary: This study found that additional occipital amyloid-beta deposition is associated with poor baseline language function and rapid cognitive deterioration in patients with Alzheimer's disease.
JOURNAL OF ALZHEIMERS DISEASE
(2023)
Article
Clinical Neurology
Kyoungwon Baik, Seun Jeon, Mincheol Park, Young-gun Lee, Phil Hyu Lee, Young H. Sohn, Byoung Seok Ye
Summary: This study compared the imaging characteristics and cutoff value of 18F-florapronol (FC119S) and 18F-florbetaben (FBB) positron emission tomography (PET) in detecting 3-amyloid positivity and Alzheimer's disease (AD) in patients with cognitive impairment. The findings showed that quantitative FC119S-PET analysis provided reliable information for detecting 3-amyloid deposition and the presence of AD.
JOURNAL OF CLINICAL NEUROLOGY
(2023)
Article
Medical Laboratory Technology
Jiansheng Lin, Weihua Lin, Yiming Lin, Weilin Peng, Zhenzhu Zheng
Summary: This study retrospectively analyzed the cases of Chinese infants with NICCD and identified multiple genetic mutations. The study also found comorbidity of NICCD and other inborn errors of metabolism in some patients.
CLINICA CHIMICA ACTA
(2024)
Review
Medical Laboratory Technology
Lihua Guan, Wei Su, Jian Zhong, Ling Qiu
Summary: Multiple myeloma is characterized by excessive production of monoclonal immunoglobulins. Routine screening methods are insufficient for detecting low levels of M proteins, but advances in mass spectrometry enable reliable detection of low abundance serum biomarkers for minimal residual disease assessment in multiple myeloma.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Liya Zhu, Chao Zhu, Jialie Jin, Jinxin Wang, Xiaojing Zhao, Rongxi Yang
Summary: This study found an association between blood-based ITGB2 methylation and coronary heart disease (CHD), with hypomethylation of ITGB2 being a risk factor for CHD. Additionally, the combination of ITGB2 methylation and conventional CHD risk factors could efficiently discriminate CHD patients from controls.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
H. Al Habobe, E. B. Haverkort, K. Nazmi, A. P. Van Splunter, R. H. H. Pieters, F. J. Bikker
Summary: Saliva diagnostics have become popular due to their non-invasive nature and patient-friendly collection process. However, the choice of saliva collection method can affect the measured levels of various biomarkers.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Abdurrahman Coskun, Anna Carobene, Ozlem Demirelce, Michele Mussap, Federica Braga, Ebru Sezer, Aasne Karine Aarsand, Sverre Sandberg, Pilar Fernandez Calle, Jorge Diaz-Garzon, Metincan Erkaya, Cihan Coskun, Esila Nur Erol, Hunkar Dag, Bill Bartlett, Mustafa Serteser, Niels Jonker, Ibrahim Unsal
Summary: In this study, BV estimates for 22 AAs were provided based on a large sample size, and it was found that there are differences in CVI estimates between males and females for most AAs, which has implications for the clinical interpretation and use of AAs.
CLINICA CHIMICA ACTA
(2024)
Review
Medical Laboratory Technology
Valentinus Besin, Farizky Martriano Humardani, Trilis Yulianti, Matthew Justyn
Summary: The study of Parkinson's Disease in Asian populations has revealed the impact of genetic variants on multiple biological pathways and highlighted shared genetic susceptibility with other diseases. These findings emphasize the importance of personalized treatment based on individual genetic profiles.
CLINICA CHIMICA ACTA
(2024)
Review
Medical Laboratory Technology
Simona Ferraro, Sara Benedetti, Savina Mannarino, Santica Marcovina, Elia Mario Biganzoli, Gianvincenzo Zuccotti
Summary: Risk stratification for cardio-vascular disease should be implemented in childhood to promote early prevention strategies, as atherosclerotic lesions can be present even in very young individuals. Evaluating pediatric CV risk factors/clinical conditions and conducting lipid profile and genetic testing can help identify children at risk of future CV events and guide appropriate therapeutic options.
CLINICA CHIMICA ACTA
(2024)
Review
Medical Laboratory Technology
Ahmad Mobed, Bita Abdi, Sajjad Masoumi, Mohammad Mikaeili, Elham Shaterian, Hamed Shaterian, Esmat Sadat Kazemi, Mahdiye Shirafkan
Summary: Reproductive biomarkers play important regulatory roles in women. The discovery and quantification of these biomarkers are clinically significant. Various detection strategies, including nanotechnology-based methods, have been developed. This article provides an in-depth introduction to the latest advances in biosensor and nanosensor research for detecting and quantitatively identifying reproductive biomarkers.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Chengfang Tang, Fang Tang, Yanna Cai, Minyi Tan, Sichi Liu, Ting Xie, Xiang Jiang, Yonglan Huang
Summary: This study proposes an effective method for screening X-ALD and evaluates the performance of newborn screening for X-ALD in Guangzhou. The LC-MS/MS method can accurately identify X-ALD through analysis of C26:0-LPC.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Lan Liu, Jiamin Wang, Xijing Liu, Jing Wang, Lin Chen, Hongmei Zhu, Jingqun Mai, Ting Hu, Shanling Liu
Summary: The 16p11.2 deletion is a common genetic cause of neurodevelopmental disorders, with prenatal and postnatal presentations including vertebral malformations and language impairment. The majority of deletions are de novo and may be associated with MAPK3 and histidine-associated metabolism.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Sneha Venkatesalu, Shanmugapriya Dilliyappan, Avanthika Satish Kumar, Thirunavukkarasu Palaniyandi, Gomathy Baskar, Maddaly Ravi, Asha Sivaji
Summary: Microfluidics is a science and technology that deals with less sample-to-more precision in vitro analysis. It has wide applications in cancer theranostics, enabling precise diagnosis and personalized treatment.
CLINICA CHIMICA ACTA
(2024)
Review
Medical Laboratory Technology
Yuanqin Zhao, Wei Deng, Zhaoyue Wang, Yanxia Wang, Hongyu Zheng, Kun Zhou, Qian Xu, Le Bai, Huiting Liu, Zhong Ren, Zhisheng Jiang
Summary: The cardiovascular system and the central nervous system exhibit a coordinated developmental process during embryonic development. Congenital heart disease (CHD) is the most common congenital disorder, and neurodevelopmental disorders (NDD) are common complications in CHD patients. Both genetic and non-genetic factors contribute to the co-occurrence of CHD and NDD. Further research should focus on identifying common molecular mechanisms underlying this co-occurrence and promoting the research and treatment of developmental disorders related to the cardiovascular and central nervous systems.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Karol Gostomczyk, Ewelina Lukaszewska, Jedrzej Borowczak, Anita Bator, Marek Zdrenka, Magdalena Bodnar, Lukasz Szylberg
Summary: Flow cytometry improves the detection of epithelial cancer cells in peritoneal and pleural fluids compared to conventional cytology. Due to similar speciflcity and higher sensitivity, flow cytometry offers a promising alternative to cytology for patient screening.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Heping Tian, Genghuan Wang, Qi Zhong, Haihang Zhou
Summary: This study found that the decline of serum ITIH4 concentrations during the early phase after aneurysmal subarachnoid hemorrhage (aSAH) was closely related to the severity and poor prognosis of the disease. Serum ITIH4 may represent a promising prognostic biomarker of aSAH.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Xueting Zhu, Yang Yu, Jun Zhang, Yuxia Zhan, Guanghua Luo, Lu Zheng
Summary: This study successfully established a 2D-PCR method for identifying HLA-B*15:02 through a two-tube reaction. This method can distinguish HLA-B*15:02 from 16 highly homologous HLA-B*15 alleles. Among 1830 samples from the clinical general population, 3 HLA-B*15:02 homozygotes and 84 HLA-B*15:02 heterozygotes were detected.
CLINICA CHIMICA ACTA
(2024)
