Article
Cell & Tissue Engineering
Tso-Fu Wang, Yu-Shan Liou, Shang-Hsien Yang, Guan-Ling Lin, Ya-Wen Chiang, Te-Sheng Lien, Chi-Cheng Li, Jen-Hung Wang, Hsin-Hou Chang, Der-Shan Sun
Summary: P-selectin is identified as a critical factor for G-CSF-induced HSC mobilization. Blocking the interaction between P-selectin and PSGL-1 can facilitate the mobilization of HSCs from bone marrow to peripheral circulation.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Hematology
Dino Mehic, Jennifer Machacek, Theresa Schramm, Lisbeth Buresch, Alexandra Kaider, Beate Eichelberger, Helmuth Haslacher, Michael Fillitz, Barbara Dixer, Tanja Flasch, Theresa Anderle, Anja Rath, Alice Assinger, Cihan Ay, Ingrid Pabinger, Johanna Gebhart
Summary: This study found that the bleeding phenotype in ITP patients is heterogeneous and usually mild. Platelet reactivity may be one of the factors underlying the mild phenotype.
THROMBOSIS RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Reza Haschemi, Lukas Maria Gockel, Gerd Bendas, Martin Schlesinger
Summary: Pancreatic cancer patients are at a higher risk of venous thrombosis, with platelets playing a key role in clot formation. The combination of tissue factor-induced thrombin formation by cancer cells and their binding to platelet P-selectin is responsible for platelet activation in AsPC-1 and Capan-2 pancreatic cancer cells. Simultaneously blocking both pathways with heparin may be an effective approach to reducing hypercoagulability in these patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Oncology
Hans-Ake Fabricius, Sarah Starzonek, Tobias Lange
Summary: Platelets, as the smallest cells in the blood stream, play crucial roles in hemostasis, inflammation, immunity, and tissue repair. In malignancies, platelets are also involved in promoting metastasis and resistance to anti-tumor treatment. The complex interaction between platelets and cancer cells is still not fully understood, although various surface molecules and transduction mechanisms have been identified.
FRONTIERS IN ONCOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Hla Nu Swe, Boonchoo Sritularak, Ponlapat Rojnuckarin, Rataya Luechapudiporn
Summary: Lusianthridin inhibited platelet aggregation induced by various stimuli and showed inhibitory effects on both COX-1 and COX-2 enzymatic activities. Its mechanisms of action may involve the arachidonic acid-thromboxane and adenylate cyclase pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Medicine, General & Internal
Philip Sommer, Michael Schreinlechner, Maria Noflatscher, Daniela Lener, Fabian Mair, Markus Theurl, Rudolf Kirchmair, Peter Marschang
Summary: The study suggests that increasing levels of sP-selectin can predict higher progression of atherosclerotic plaques. Serial measurements of sP-selectin may serve as an easily measurable biomarker for peripheral atherosclerotic plaque progression.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Hematology
Pandarisamy Sundaravadivel, Rita Christopher, Nitin Ramanujam, Sadanandavalli Retnaswami Chandra
Summary: In this study, serum thromboxane B2 (sTXB2) measurement was suggested as an alternative method to identify aspirin-treated ischemic stroke patients at risk of enhanced platelet reactivity and subsequent vascular events. Results showed that sTXB2, with a cut-off level of >4.15 ng/mL, could distinguish patients with elevated platelet reactivity with a sensitivity of 84.3%, and was in fair agreement with the LTA-based classification of patients. On the other hand, soluble P-selectin levels showed no discriminatory ability in identifying at-risk patients.
THROMBOSIS RESEARCH
(2021)
Article
Medicine, Research & Experimental
Agata Rolnik, Anna Stochmal, Beata Olas
Summary: Extracts from plants of the Asteraceae family, such as dandelion, have been found to possess antioxidant and anti-platelet activity. However, little is known about the effects of extracts from other Asteraceae plants, such as chicory leaves, lettuce leaves, and Jerusalem artichoke roots, on the hemostatic system. The study found that dandelion root extract and fruit extracts have the strongest anti-platelet potential in whole blood, while red lettuce leaves and Jerusalem artichoke roots showed the strongest anti-platelet activity in washed blood platelets.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Biotechnology & Applied Microbiology
Chia-Hung Liu, Pei-Ru Jheng, Lekha Rethi, Chandraiah Godugu, Ching Yi Lee, Yan-Ting Chen, Hieu Trung Nguyen, Er-Yuan Chuang
Summary: Thrombotic vascular disorders have a negative impact on public health, and current medication protocols for preventing thrombus formation are inadequate. This study developed self-assembling nanoparticles that can be delivered directly to thrombi, preventing recurrence and emitting visible signals.
JOURNAL OF NANOBIOTECHNOLOGY
(2023)
Article
Medicine, General & Internal
Bernadeta Chyrchel, Olga Kruszelnicka, Ewa Wieczorek-Surdacka, Andrzej Surdacki
Summary: The degree of platelet inhibition in patients receiving dual antiplatelet therapy (DAPT) can affect cardiovascular outcomes after acute coronary syndromes (ACS) and/or percutaneous coronary intervention. This study aimed to find correlates of residual ex vivo platelet reactivity and circulating soluble P-selectin (sP-selectin), which is an indicator of platelet activation in patients treated with DAPT using ticagrelor. The study found that lower residual platelet aggregation was associated with better control of low-density lipoprotein cholesterol (LDL-c) in patients undergoing ticagrelor-based DAPT.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Biochemical Research Methods
Chi-Ju Kim, Jungmin Kim, Jonathan Sabate del Rio, Dong Yeob Ki, Junyoung Kim, Yoon-Kyoung Cho
Summary: Platelet function tests are crucial in diagnosing inherited platelet function disorders and monitoring antiplatelet therapies. Light transmission aggregometry (LTA) is considered the gold standard, but lacks standardization and requires a large volume of blood. A centrifugal microfluidic disc has been developed to automate LTA testing from a small blood sample, producing more precise results in a shorter time.
Review
Microbiology
Antoine Mariotti, Amina Ezzeroug Ezzraimi, Laurence Camoin-Jau
Summary: Despite improvements in the prognosis of sepsis, it remains a common cause of hospitalization and mortality in Western countries, leading to various complications including thrombotic complications. Escherichia coli, a Gram-negative bacillus, is frequently implicated in sepsis and can induce platelet activation. The mechanisms of platelet activation and the use of antiplatelet agents in sepsis are still controversial.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Cell Biology
Benedetta Izzi, Alessandro Gialluisi, Francesco Gianfagna, Sabatino Orlandi, Amalia De Curtis, Sara Magnacca, Simona Costanzo, Augusto Di Castelnuovo, Maria Benedetta Donati, Giovanni de Gaetano, Marc F. Hoylaerts, Chiara Cerletti, Licia Iacoviello
Summary: Platelet distribution width (PDW) can serve as a simple and useful marker for platelet activation, specifically P-selectin dependent, with no sex differences, while mean platelet volume (MPV) shows no significant association. Further studies in larger cohorts are needed to fully understand the predictive value of PDW in thrombo-inflammatory conditions.
Article
Biochemistry & Molecular Biology
Bibian M. E. Tullemans, Alicia Veninga, Delia Fernandez, Maureen J. B. Aarts, Johannes A. Eble, Paola E. J. van der Meijden, Johan W. M. Heemskerk, Marijke J. E. Kuijpers
Summary: This study compared the antiplatelet properties of eight TKIs for potential repurposing as antiplatelet drugs. TKIs such as dasatinib, fostamatinib, sunitinib, and pazopanib exhibited strong inhibitory effects on platelet function and thrombus formation, making them promising candidates for further evaluation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Hematology
Slimane Allali, Fabienne Marquant, Rachel Rignault-Bricard, Melissa Taylor, Josephine Brice, Mariane de Montalembert, Thiago Trovati Maciel, Caroline Elie, Olivier Hermine
Summary: Plasma histamine levels are increased in sickle cell disease (SCD) patients, and famotidine, a H2 receptor antagonist, could reduce P-selectin expression and vaso-occlusion. A study was conducted on SCD children, and it was found that oral famotidine significantly reduced plasma P-selectin levels in 28 patients without adverse events. Further randomized controlled trials are needed to assess the efficacy of famotidine in preventing vaso-occlusion in SCD.
BRITISH JOURNAL OF HAEMATOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Cristina Morelli, Vincenzo Formica, Silvia Riondino, Antonio Russo, Patrizia Ferroni, Fiorella Guadagni, Mario Roselli
Summary: The study discusses the potential link between RAS, BRAF, and microsatellite instability with the efficacy of oxaliplatin and irinotecan in chemotherapy. KRAS mutation increases sensitivity to oxaliplatin, while limited data is available on the efficacy of BRAF mutations. Irinotecan shows enhanced cytotoxicity to MSI cell lines due to the involvement of mismatch repair components in DNA repair processes.
CURRENT MEDICINAL CHEMISTRY
(2021)
Article
Medicine, General & Internal
Vincenzo Formica, Jessica Lucchetti, Elena Doldo, Silvia Riondino, Cristina Morelli, Renato Argiro, Nicola Renzi, Daniele Nitti, Antonella Nardecchia, Emanuela Dell'Aquila, Patrizia Ferroni, Fiorella Guadagni, Giampiero Palmieri, Augusto Orlandi, Mario Roselli
Summary: This study demonstrates that mutational analysis through plasma can better predict survival outcomes for colorectal cancer patients, compared to relying solely on tumor tissue analysis. Plasma mutations further refine prognosis and can help guide clinical management.
JOURNAL OF CLINICAL MEDICINE
(2021)
Review
Biochemistry & Molecular Biology
Antonio Jesus Banegas-Luna, Jorge Pena-Garcia, Adrian Iftene, Fiorella Guadagni, Patrizia Ferroni, Noemi Scarpato, Fabio Massimo Zanzotto, Andres Bueno-Crespo, Horacio Perez-Sanchez
Summary: Artificial intelligence has made astonishing progress in medicine, especially in cancer diagnosis and therapies. Machine learning models and in-silico tools are widely used, yet the interpretability of machine learning predictions for doctors is still an issue that needs improvement.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Editorial Material
Oncology
Patrizia Ferroni, Fiorella Guadagni, Mario Roselli
Meeting Abstract
Oncology
C. Morelli, V. Formica, E. Doldo, S. Riondino, M. Rofei, G. Palmieri, A. Orlandi, M. Roselli
ANNALS OF ONCOLOGY
(2021)
Meeting Abstract
Oncology
M. Rofei, C. Morelli, S. Riondino, S. Guerriero, G. Parisi, R. Argiro, D. Nitti, F. Guadagni, V. Formica, M. Roselli
ANNALS OF ONCOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Giovanni Maria Iannantuono, Francesco Torino, Roberto Rosenfeld, Simona Guerriero, Manuela Carlucci, Stefano Sganga, Barbara Capotondi, Silvia Riondino, Mario Roselli
Summary: Precision medicine has ushered in a new era in anti-cancer drug development by focusing on biomarkers predictive of treatment response regardless of tumor histology. Although there have been histology-agnostic drugs approved by the FDA in recent years, the efficacy data for metastatic castration-resistant prostate cancer patients is still limited. Therefore, large multi-institutional registries are needed to collect real-world data.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Health Care Sciences & Services
Roberto Rosenfeld, Silvia Riondino, Vincenzo Formica, Francesco Torino, Eugenio Martuscelli, Mario Roselli
Summary: Breast cancer is the most common type of cancer in women, and the chemotherapy drug doxorubicin can cause heart toxicity. Early diagnosis of heart toxicity is important to prevent complications. Non-coding RNA, specifically microRNA and circular RNA, are being researched as potential biomarkers for early detection. Further studies are needed to understand the mechanisms and develop reliable detection methods.
JOURNAL OF PERSONALIZED MEDICINE
(2022)
Review
Oncology
Valentina Russo, Eleonora Lallo, Armelle Munnia, Miriana Spedicato, Luca Messerini, Romina D'Aurizio, Elia Giuseppe Ceroni, Giulia Brunelli, Antonio Galvano, Antonio Russo, Ida Landini, Stefania Nobili, Marcello Ceppi, Marco Bruzzone, Fabio Cianchi, Fabio Staderini, Mario Roselli, Silvia Riondino, Patrizia Ferroni, Fiorella Guadagni, Enrico Mini, Marco Peluso
Summary: Metastatic colorectal cancer (mCRC) has a high incidence and mortality rate. Innovative biomarkers have been developed for predicting treatment response. By analyzing specific narrative publications, this study examines the ability of learning methods to build predictive models for mCRC patients, either for chemotherapy alone or combined with targeted therapy. The results show promising outcomes in predicting treatment response or toxic side-effects. Radiomics and molecular biomarkers are able to accurately identify responders and non-responders in the majority of patients.
Article
Biochemistry & Molecular Biology
Patrizia Ferroni, Raffaele Palmirotta, Gabriella Egeo, Cinzia Aurilia, Maria Giovanna Valente, Antonella Spila, Alberto Pierallini, Piero Barbanti, Fiorella Guadagni
Summary: The study investigates the association between white matter hyperintensities (WMHs) and genetic pro-inflammatory/pro-oxidative status in migraine patients. The results show no significant difference in gene polymorphisms between patients and controls. However, specific alleles of the lymphotoxin alpha (LTA) and superoxide dismutase 1 (SOD1) genes are associated with WMHs. This suggests that an imbalance between pro-inflammatory/pro-oxidative and antioxidant events may contribute to the development of WMHs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Silvia Riondino, Vincenzo Formica, Elena Valenzi, Cristina Morelli, Valeria Flaminio, Ilaria Portarena, Francesco Torino, Mario Roselli
Summary: This study aimed to identify a cut-off point of body mass index (BMI) that would have a significant prognostic impact on stage I-II BC patients receiving AI treatment. The results showed that a BMI cut-off >29 was associated with a deteriorating disease-free survival (DFS) in AI-treated BC patients, indicating that obesity is a risk factor for recurrence in breast cancer.
Article
Medical Laboratory Technology
Jiansheng Lin, Weihua Lin, Yiming Lin, Weilin Peng, Zhenzhu Zheng
Summary: This study retrospectively analyzed the cases of Chinese infants with NICCD and identified multiple genetic mutations. The study also found comorbidity of NICCD and other inborn errors of metabolism in some patients.
CLINICA CHIMICA ACTA
(2024)
Review
Medical Laboratory Technology
Lihua Guan, Wei Su, Jian Zhong, Ling Qiu
Summary: Multiple myeloma is characterized by excessive production of monoclonal immunoglobulins. Routine screening methods are insufficient for detecting low levels of M proteins, but advances in mass spectrometry enable reliable detection of low abundance serum biomarkers for minimal residual disease assessment in multiple myeloma.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Liya Zhu, Chao Zhu, Jialie Jin, Jinxin Wang, Xiaojing Zhao, Rongxi Yang
Summary: This study found an association between blood-based ITGB2 methylation and coronary heart disease (CHD), with hypomethylation of ITGB2 being a risk factor for CHD. Additionally, the combination of ITGB2 methylation and conventional CHD risk factors could efficiently discriminate CHD patients from controls.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
H. Al Habobe, E. B. Haverkort, K. Nazmi, A. P. Van Splunter, R. H. H. Pieters, F. J. Bikker
Summary: Saliva diagnostics have become popular due to their non-invasive nature and patient-friendly collection process. However, the choice of saliva collection method can affect the measured levels of various biomarkers.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Abdurrahman Coskun, Anna Carobene, Ozlem Demirelce, Michele Mussap, Federica Braga, Ebru Sezer, Aasne Karine Aarsand, Sverre Sandberg, Pilar Fernandez Calle, Jorge Diaz-Garzon, Metincan Erkaya, Cihan Coskun, Esila Nur Erol, Hunkar Dag, Bill Bartlett, Mustafa Serteser, Niels Jonker, Ibrahim Unsal
Summary: In this study, BV estimates for 22 AAs were provided based on a large sample size, and it was found that there are differences in CVI estimates between males and females for most AAs, which has implications for the clinical interpretation and use of AAs.
CLINICA CHIMICA ACTA
(2024)
Review
Medical Laboratory Technology
Valentinus Besin, Farizky Martriano Humardani, Trilis Yulianti, Matthew Justyn
Summary: The study of Parkinson's Disease in Asian populations has revealed the impact of genetic variants on multiple biological pathways and highlighted shared genetic susceptibility with other diseases. These findings emphasize the importance of personalized treatment based on individual genetic profiles.
CLINICA CHIMICA ACTA
(2024)
Review
Medical Laboratory Technology
Simona Ferraro, Sara Benedetti, Savina Mannarino, Santica Marcovina, Elia Mario Biganzoli, Gianvincenzo Zuccotti
Summary: Risk stratification for cardio-vascular disease should be implemented in childhood to promote early prevention strategies, as atherosclerotic lesions can be present even in very young individuals. Evaluating pediatric CV risk factors/clinical conditions and conducting lipid profile and genetic testing can help identify children at risk of future CV events and guide appropriate therapeutic options.
CLINICA CHIMICA ACTA
(2024)
Review
Medical Laboratory Technology
Ahmad Mobed, Bita Abdi, Sajjad Masoumi, Mohammad Mikaeili, Elham Shaterian, Hamed Shaterian, Esmat Sadat Kazemi, Mahdiye Shirafkan
Summary: Reproductive biomarkers play important regulatory roles in women. The discovery and quantification of these biomarkers are clinically significant. Various detection strategies, including nanotechnology-based methods, have been developed. This article provides an in-depth introduction to the latest advances in biosensor and nanosensor research for detecting and quantitatively identifying reproductive biomarkers.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Chengfang Tang, Fang Tang, Yanna Cai, Minyi Tan, Sichi Liu, Ting Xie, Xiang Jiang, Yonglan Huang
Summary: This study proposes an effective method for screening X-ALD and evaluates the performance of newborn screening for X-ALD in Guangzhou. The LC-MS/MS method can accurately identify X-ALD through analysis of C26:0-LPC.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Lan Liu, Jiamin Wang, Xijing Liu, Jing Wang, Lin Chen, Hongmei Zhu, Jingqun Mai, Ting Hu, Shanling Liu
Summary: The 16p11.2 deletion is a common genetic cause of neurodevelopmental disorders, with prenatal and postnatal presentations including vertebral malformations and language impairment. The majority of deletions are de novo and may be associated with MAPK3 and histidine-associated metabolism.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Sneha Venkatesalu, Shanmugapriya Dilliyappan, Avanthika Satish Kumar, Thirunavukkarasu Palaniyandi, Gomathy Baskar, Maddaly Ravi, Asha Sivaji
Summary: Microfluidics is a science and technology that deals with less sample-to-more precision in vitro analysis. It has wide applications in cancer theranostics, enabling precise diagnosis and personalized treatment.
CLINICA CHIMICA ACTA
(2024)
Review
Medical Laboratory Technology
Yuanqin Zhao, Wei Deng, Zhaoyue Wang, Yanxia Wang, Hongyu Zheng, Kun Zhou, Qian Xu, Le Bai, Huiting Liu, Zhong Ren, Zhisheng Jiang
Summary: The cardiovascular system and the central nervous system exhibit a coordinated developmental process during embryonic development. Congenital heart disease (CHD) is the most common congenital disorder, and neurodevelopmental disorders (NDD) are common complications in CHD patients. Both genetic and non-genetic factors contribute to the co-occurrence of CHD and NDD. Further research should focus on identifying common molecular mechanisms underlying this co-occurrence and promoting the research and treatment of developmental disorders related to the cardiovascular and central nervous systems.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Karol Gostomczyk, Ewelina Lukaszewska, Jedrzej Borowczak, Anita Bator, Marek Zdrenka, Magdalena Bodnar, Lukasz Szylberg
Summary: Flow cytometry improves the detection of epithelial cancer cells in peritoneal and pleural fluids compared to conventional cytology. Due to similar speciflcity and higher sensitivity, flow cytometry offers a promising alternative to cytology for patient screening.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Heping Tian, Genghuan Wang, Qi Zhong, Haihang Zhou
Summary: This study found that the decline of serum ITIH4 concentrations during the early phase after aneurysmal subarachnoid hemorrhage (aSAH) was closely related to the severity and poor prognosis of the disease. Serum ITIH4 may represent a promising prognostic biomarker of aSAH.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Xueting Zhu, Yang Yu, Jun Zhang, Yuxia Zhan, Guanghua Luo, Lu Zheng
Summary: This study successfully established a 2D-PCR method for identifying HLA-B*15:02 through a two-tube reaction. This method can distinguish HLA-B*15:02 from 16 highly homologous HLA-B*15 alleles. Among 1830 samples from the clinical general population, 3 HLA-B*15:02 homozygotes and 84 HLA-B*15:02 heterozygotes were detected.
CLINICA CHIMICA ACTA
(2024)