Article
Cardiac & Cardiovascular Systems
Ran Zhang, Yue Zhong, Si-Yu Long, Qin-Ni Yang, Bin Zhou, Li Rao
Summary: The study suggests that CDK8 gene polymorphisms may be associated with DCM susceptibility and prognosis in the Chinese Han population, with specific genotypes correlating with poor prognosis in DCM patients.
CARDIOVASCULAR DIAGNOSIS AND THERAPY
(2021)
Article
Biochemistry & Molecular Biology
Jaroslaw Goracy, Anna Bogacz, Izabela Uzar, Marlena Wolek, Malgorzata Lochynska, Pawel Zietek, Boguslaw Czerny, Aneta Cymbaluk-Ploska, Piotr Modliborski, Adam Kaminski
Summary: This study aimed to evaluate the association between NQO1 gene 609C > T polymorphism and colorectal cancer risk in the Polish population, finding that carriers of the TT genotype have an elevated risk for colorectal cancer. The analysis also revealed a significant increase in colorectal cancer risk in carriers of the TT genotype, particularly related to tumor stage and location.
Article
Biochemistry & Molecular Biology
Ruslan Kalendar, Alexandr V. Shustov, Ilyas Akhmetollayev, Ulykbek Kairov
Summary: This article introduces a bioinformatic tool for PCR-based genotyping assays, which allows design of probe sequences, discrimination of allelic variants, and enhances reaction specificity and discriminative power, offering greater likelihood of research success.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Biochemical Research Methods
Weidu Song, Chuanhao Zhang, Huichao Lin, Taiyi Zhang, Haixia Liu, Xiaowen Huang
Summary: The proposed real-time rotary PCR reaction system (RRP) meets the requirements of low cost, low-power consumption, potential for miniaturization, and portability. Experimental and theoretical results demonstrate its feasibility in the rapid quantitative detection of Pseudomonas aeruginosa and have broad application prospects in bacteria detection and food safety.
Article
Biochemical Research Methods
Shaolei Huang, Yiquan An, Bangchao Xi, Xianglian Gong, Zhongfu Chen, Shan Shao, Shengxiang Ge, Jun Zhang, Dongxu Zhang, Ningshao Xia
Summary: Nucleic acid detection is a method that directly identifies the presence of pathogenic microorganisms and has advantages of high sensitivity, commendable specificity, and a short window period. A real-time PCR system based on multiple temperature zones was proposed to solve the time-consuming problem, enabling fast detection speed and high sensitivity under extreme conditions.
Article
Multidisciplinary Sciences
Daan van Beek, Job Verdonschot, Kasper Derks, Han Brunner, Theo M. M. de Kok, Ilja C. W. Arts, Stephane Heymans, Martina Kutmon, Michiel Adriaens
Summary: This study developed an analysis pipeline for detecting allele-specific expression, which can be used to study cis-regulatory variation and its impact on complex genetic disorders such as dilated cardiomyopathy. The pipeline was applied to a cohort of 87 well-phenotyped patients and revealed an overrepresentation of known disease-associated genes. Additionally, novel genes that have not been identified through conventional methods were also discovered. The pipeline provides various analysis tools and visualizations for candidate gene identification and biological insight.
SCIENTIFIC REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Fumie Takei, Misaki Akiyama, Minori Dateki
Summary: The study introduces a novel RT-Hpro-PCR technique for accurate detection of miRNAs with similar sequences. By enhancing the specificity of RT through the use of a DNA-tagged RT primer and hairpin probe structure, this method shows potential for exploring the roles of miRNAs in biological processes.
BIOORGANIC & MEDICINAL CHEMISTRY
(2021)
Article
Virology
Imene Ben Dhifallah, Kaouther Ayouni, Haifa Jmel, Wafa Kammoun, Kamel Hamzaoui, Amel Sadraoui, Henda Triki
Summary: The study revealed that the IL-12B gene polymorphism is significantly associated with an increased risk of chronic hepatitis B (CHB) in patients, particularly in males under the age of 50.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Article
Multidisciplinary Sciences
Jae Jong Kim, Hyoung-Min Park, A. Young Kyoung, In Kyung Park, Si-Kyu Lim, Byoung Chul Park
Summary: Genetic mutations like SNP are common in genetic disorders and cancers, and PCR methods are widely used for SNP detection. The specifically designed PCR platform STexS can efficiently distinguish small amounts of SNP mixed in normal DNA, aiding in targeting potential SNPs or genetically mutated biomarkers in human clinical samples.
SCIENTIFIC REPORTS
(2021)
Article
Biology
Priya Jarwar, Yar Muhammad Waryah, Muhammad Rafiq, Ali Muhammad Waryah
Summary: This study analyzed the association between SNP variants in CRYAA and CRYAB genes and Congenital Cataract. The results showed that the rs13053109 G > C variant in CRYAA gene is associated with increased risk of congenital cataract in infants. Haplotype analysis indicated weak linkage disequilibrium between the SNPs rs3761382, rs7278468, and rs13051039 of CRYAA gene. These findings suggest that the variation in CRYAA gene may be a risk factor for congenital cataract in infants.
SAUDI JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Review
Cardiac & Cardiovascular Systems
Pau Bosch-Nicolau, Juan Espinosa-Pereiro, Fernando Salvador, Adrian Sanchez-Montalva, Israel Molina
Summary: This study conducted a systematic review of the available literature and found that a correlation between PCR-detectable parasitemia and chronic chagasic cardiomyopathy (CCC) could not be established. Due to the heterogeneity across studies and the risk of bias in some of the methods, further research is needed to investigate the relationship between the two.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Masaaki Sudo, Masahiro Osakabe
Summary: PCR techniques are commonly used to estimate allele frequency in populations, with variation in DNA yields and measurement errors causing uncertainty in qPCR results. A statistical model was developed to estimate allele frequency and confidence intervals using Delta Delta Cq data from multiple bulk samples. The model showed unbiased estimates compared to simple averaging, with suggestions that dividing bulk samples for more precision or pooling into fewer samples for cost efficiency may yield comparable results.
MOLECULAR ECOLOGY RESOURCES
(2022)
Article
Chemistry, Multidisciplinary
Sudheendran Mavila, Heidi R. Culver, Alex J. Anderson, Tania R. Prieto, Christopher N. Bowman
Summary: An athermal approach using self-immolative thioester linked nucleic acids (TENA) for mRNA enrichment from total RNA has shown comparable performance to a commercial kit. The method is compatible with downstream applications such as RT-qPCR for gene expression analysis.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Ophthalmology
Satoko Nakano, Yasuhiro Tomaru, Toshiaki Kubota, Hiroshi Takase, Manabu Mochizuki, Norio Shimizu, Sunao Sugita
Summary: The Direct Strip PCR test offers rapid and accurate detection of infectious pathogens causing uveitis. It shows high concordance with qPCR results, even when used by beginners, indicating its reliability for clinical use. Additionally, it can be used for intraoperative diagnosis, improving the prognosis of infectious diseases.
Article
Medicine, General & Internal
Jeong-Hwa Kim, Jae-Woon Oh, Young Lee, Jeong-Ho Yun, Seong-Ho Choi, Dong-Woon Lee
Summary: This study found that detecting 18 bacterial species in mouth-rinse samples can be used to diagnose periodontitis, with Treponema denticola, Prevotella nigrescens, and Streptococcus mitis significantly associated with severe periodontitis. Further research is needed to assess the potential of oral-rinsing solutions in indicating oral infection risk and improving oral hygiene.
JOURNAL OF CLINICAL MEDICINE
(2021)
Review
Clinical Neurology
Yuxin Shen, Zhengyi Huang, Ruiqing Yang, Yunlong Chen, Qiang Wang, Linbo Gao
Summary: This review discusses the concept, characteristics, classification, function, and potential roles of enhancer RNAs (eRNAs) in brain diseases. Despite the small proportion of eRNAs, they play important roles in enhancer activity and transcriptional activation.
Article
Multidisciplinary Sciences
Lei Luo, Zifeng Yang, Jingyi Liang, Yu Ma, Hui Wang, Chitin Hon, Mei Jiang, Zhengshi Lin, Wenda Guan, Zhitong Mai, Yongming Li, Kailin Mai, Zhiqi Zeng, Chuanmeizi Tu, Jian Song, Bin Liu, Yong Liu, Jianfeng He, Huiyuan Li, Bosheng Li, Hang Dong, Yutian Miao, Shujun Fan, Lirui Fan, Xingyi Liang, Ke Li, Chun Chen, Huihong Deng, Zhicong Yang, Nanshan Zhong
Summary: This study analyzed the transmission characteristics of the Delta variant in Guangzhou and found that it spread rapidly within a short period of time. By redefining close contact and implementing measures such as health-code usage, the proportion of confirmed cases discovered from close contacts significantly increased. The establishment of makeshift inflatable laboratories and mass testing effectively controlled the Delta epidemic in Guangzhou.
NATIONAL SCIENCE REVIEW
(2022)
Article
Genetics & Heredity
Rangran Zhang, Yu Tan, Li Wang, Hui Jian, Jing Zhu, Yuanyuan Xiao, Mengyu Tan, Jiaming Xue, Fan Yang, Weibo Liang
Summary: A new set of SNP-SNP microhaplotypes with short amplicons was developed for detecting minor DNA in complex mixtures, showing potential for mixture studies and noninvasive prenatal paternity testing. The SNP-SNP strategy based on the CE platform proved to be useful for UDM detection and NIPPT.
FRONTIERS IN GENETICS
(2022)
Article
Medicine, General & Internal
Wei Li, Shuai Yang, Peng Xu, Dapeng Zhang, Ying Tong, Lu Chen, Ben Jia, Ang Li, Cheng Lian, Daoping Ru, Baolong Zhang, Mengxing Liu, Cancan Chen, Weihui Fu, Songhua Yuan, Chenjian Gu, Lu Wang, Wenxuan Li, Ying Liang, Zhicong Yang, Xiaoguang Ren, Shaoxuan Wang, Xiaoyan Zhang, Yuanlin Song, Youhua Xie, Hongzhou Lu, Jianqing Xu, Hailin Wang, Wenqiang Yu
Summary: This study reveals the mechanism of interaction between SARS-CoV-2 virus and host and the development of various symptoms in COVID-19. The study found that the virus shares similar RNA sequences with the human genome, and these sequences can upregulate the expression of host genes, including hyaluronan and cytokine genes, through direct genomic interaction. The study also found that severe COVID-19 patients have elevated levels of hyaluronan, and hymecromone can inhibit hyaluronan production, showing potential as a therapeutic option.
Article
Environmental Sciences
Shujun Fan, Wenru Feng, Ziyan Zhou, Yuming Guo, Zhenxiang Xue, Jun Yuan, Yuzhong Wang, Congcong Li, Yi Zhong, Weiyun He, Guanghui Dong, Boyi Yang, Zhicong Yang, Zhoubin Zhang
Summary: The study found that living in greener areas was associated with lower waist circumference and body mass index levels among rural Uyghur adults in China. Higher residential greenness levels were also linked to a lower odds of peripheral overweight/obesity prevalence, especially in older adults.
ENVIRONMENTAL RESEARCH
(2022)
Article
Medicine, Legal
Jiaming Xue, Shengqiu Qu, Mengyu Tan, Yuanyuan Xiao, Ranran Zhang, Dezhi Chen, Meili Lv, Yiming Zhang, Lin Zhang, Weibo Liang
Summary: Microhaplotypes (MHs) are a promising new type of forensic markers that have advantages such as low mutation rates, lack of stutter artifacts, and short amplicons. They have improved human identification, kinship analysis, ancestry prediction, and mixture deconvolution capabilities. While there are some public databases available for MHs, there is a need for a more comprehensive database that integrates information from other databases. This study successfully established a dual-SNP MH database (D-SNPsDB) for 26 populations, providing basic data such as physical positions, allele frequencies, and variant information.
INTERNATIONAL JOURNAL OF LEGAL MEDICINE
(2022)
Review
Biochemistry & Molecular Biology
Yuwen Sun, Manrui Li, Shuqiang Cao, Yang Xu, Peiyan Wu, Shuting Xu, Qian Pan, Yadong Guo, Yi Ye, Zheng Wang, Hao Dai, Xiaoqi Xie, Xiameng Chen, Weibo Liang
Summary: Optogenetics is a promising method for controlling cellular activity, particularly in the field of brain injury. This review discusses the latest research on optogenetics, focusing on its improvement, existing challenges, and potential clinical applications. It highlights the importance of advancing optogenetics in the study of brain injury and explores the future prospects of this technology.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Shuangshuang Wang, Feng Song, Haoyu Gu, Zhilong Shu, Xiaowen Wei, Ke Zhang, Yuxiang Zhou, Lanrui Jiang, Zefei Wang, Jienan Li, Haibo Luo, Weibo Liang
Summary: This study investigated the gut microbiota of healthy adults with different traits. The results showed differences in gut microbiota composition between male and female subjects. Additionally, the gut microbiota composition was able to distinguish between different BMI groups. At the phylum level, specific phyla were found to be increased in different BMI groups for both males and females. At the genus level, several distinct genera were identified across BMI groups. The accuracy of predicting body type using a linear regression model was 74%.
MICROBIAL CELL FACTORIES
(2022)
Article
Veterinary Sciences
Yuehong Wei, Dapeng Li, Zhicong Yang, Kuncai Chen, Xinhong Pan, Jianmin Xu, Shouyi Chen
Summary: A multidisciplinary One Health intervention was effective in controlling the rabies outbreak and addressing the issue of attacks by stray dogs.
VETERINARY MEDICINE AND SCIENCE
(2023)
Article
Neurosciences
Huiqing Wang, Qiang Wang, Xiao Xiao, Xiaolei Luo, Linbo Gao
CURRENT NEUROPHARMACOLOGY
(2023)
Article
Environmental Sciences
Li-Xin Hu, Shujun Fan, Yinghua Ma, Xiao-Xuan Liu, Wen-Wen Bao, Yuming Guo, Li-Wen Hu, Gongbo Chen, Xiao-Wen Zeng, Zhiyong Zou, Bo-Yi Yang, Jun Ma, Zhicong Yang, Ya-Jun Chen, Guang-Hui Dong
Summary: There is a positive association between greenspace surrounding schools and lipid levels and dyslipidemia prevalence among Chinese children and teenagers. The association is stronger in students with lower education levels and those aged <= 12 years. Part of the association can be explained by lower levels of PM2.5 in the air.
ENVIRONMENTAL POLLUTION
(2023)
Article
Multidisciplinary Sciences
Manrui Li, Xiameng Chen, Qiuyun Yang, Shuqiang Cao, Steven Wyler, Ruixuan Yuan, Lingxuan Zhang, Miao Liao, Meili Lv, Feng Wang, Yadong Guo, Jihong Zhou, Lin Zhang, Xiaoqi Xie, Weibo Liang
Summary: Explosive blast-related traumatic brain injuries (bTBIs) are common in war zones and terrorist attacks, but there is a lack of specific strategies for diagnosing and treating them. This study uses single-nucleus RNA-sequencing of the sub-ventricular zone (SVZ) to explore the mechanisms underlying the pathogenesis of bTBIs and provide a reference for future studies on diagnosis and treatment.
Article
Genetics & Heredity
Ranran Zhang, Jiaming Xue, Mengyu Tan, Dezhi Chen, Yuanyuan Xiao, Guihong Liu, Yazi Zheng, Qiushuo Wu, Miao Liao, Meili Lv, Shengqiu Qu, Weibo Liang
Summary: In this study, a panel of 50 Microhaplotypes (MHs) was constructed and analyzed using the Multiseq multiple polymerase chain reaction (multi-PCR) targeted capture sequencing protocol. It was found that this panel has the advantages of both short tandem repeats (STRs) and single nucleotide polymorphisms (SNPs), with high sensitivity and specificity. It also showed measurable polymorphism among a group of Han individuals and had high detection rates for highly degraded samples and mixtures.
Article
Microbiology
Shujuan Wang, Yanjie Shang, Zhiyun Pi, Ziqi Zhou, Xiangyan Zhang, Lipin Ren, Weibo Liang, Yadong Guo, Jifeng Cai, Changquan Zhang
Summary: A mild traumatic brain injury (mTBI) can increase the risk of neurodegenerative-related disease, and serious long-term outcomes are often overlooked. Recent research has revealed that the oral cavity and fecal microbiota play a fundamental role in deeply interconnecting the gut and brain injury. We investigated the relationship between the temporal changes of the oral cavity and fecal bacterial communities with damage identification and post-injury time estimation after mTBI.
Article
Multidisciplinary Sciences
Lingxuan Zhang, Qiuyun Yang, Ruixuan Yuan, Manrui Li, Meili Lv, Lin Zhang, Xiaoqi Xie, Weibo Liang, Xiameng Chen
Summary: This study employed single-nucleus RNA sequencing to sequence the hippocampal nuclei of mice from bTBI and sham groups, obtaining reliable gene expression data. The dataset holds important reference value for exploring secondary hippocampal injury and neurogenesis mechanisms following bTBI.
Article
Medical Laboratory Technology
Jiansheng Lin, Weihua Lin, Yiming Lin, Weilin Peng, Zhenzhu Zheng
Summary: This study retrospectively analyzed the cases of Chinese infants with NICCD and identified multiple genetic mutations. The study also found comorbidity of NICCD and other inborn errors of metabolism in some patients.
CLINICA CHIMICA ACTA
(2024)
Review
Medical Laboratory Technology
Lihua Guan, Wei Su, Jian Zhong, Ling Qiu
Summary: Multiple myeloma is characterized by excessive production of monoclonal immunoglobulins. Routine screening methods are insufficient for detecting low levels of M proteins, but advances in mass spectrometry enable reliable detection of low abundance serum biomarkers for minimal residual disease assessment in multiple myeloma.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Liya Zhu, Chao Zhu, Jialie Jin, Jinxin Wang, Xiaojing Zhao, Rongxi Yang
Summary: This study found an association between blood-based ITGB2 methylation and coronary heart disease (CHD), with hypomethylation of ITGB2 being a risk factor for CHD. Additionally, the combination of ITGB2 methylation and conventional CHD risk factors could efficiently discriminate CHD patients from controls.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
H. Al Habobe, E. B. Haverkort, K. Nazmi, A. P. Van Splunter, R. H. H. Pieters, F. J. Bikker
Summary: Saliva diagnostics have become popular due to their non-invasive nature and patient-friendly collection process. However, the choice of saliva collection method can affect the measured levels of various biomarkers.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Abdurrahman Coskun, Anna Carobene, Ozlem Demirelce, Michele Mussap, Federica Braga, Ebru Sezer, Aasne Karine Aarsand, Sverre Sandberg, Pilar Fernandez Calle, Jorge Diaz-Garzon, Metincan Erkaya, Cihan Coskun, Esila Nur Erol, Hunkar Dag, Bill Bartlett, Mustafa Serteser, Niels Jonker, Ibrahim Unsal
Summary: In this study, BV estimates for 22 AAs were provided based on a large sample size, and it was found that there are differences in CVI estimates between males and females for most AAs, which has implications for the clinical interpretation and use of AAs.
CLINICA CHIMICA ACTA
(2024)
Review
Medical Laboratory Technology
Valentinus Besin, Farizky Martriano Humardani, Trilis Yulianti, Matthew Justyn
Summary: The study of Parkinson's Disease in Asian populations has revealed the impact of genetic variants on multiple biological pathways and highlighted shared genetic susceptibility with other diseases. These findings emphasize the importance of personalized treatment based on individual genetic profiles.
CLINICA CHIMICA ACTA
(2024)
Review
Medical Laboratory Technology
Simona Ferraro, Sara Benedetti, Savina Mannarino, Santica Marcovina, Elia Mario Biganzoli, Gianvincenzo Zuccotti
Summary: Risk stratification for cardio-vascular disease should be implemented in childhood to promote early prevention strategies, as atherosclerotic lesions can be present even in very young individuals. Evaluating pediatric CV risk factors/clinical conditions and conducting lipid profile and genetic testing can help identify children at risk of future CV events and guide appropriate therapeutic options.
CLINICA CHIMICA ACTA
(2024)
Review
Medical Laboratory Technology
Ahmad Mobed, Bita Abdi, Sajjad Masoumi, Mohammad Mikaeili, Elham Shaterian, Hamed Shaterian, Esmat Sadat Kazemi, Mahdiye Shirafkan
Summary: Reproductive biomarkers play important regulatory roles in women. The discovery and quantification of these biomarkers are clinically significant. Various detection strategies, including nanotechnology-based methods, have been developed. This article provides an in-depth introduction to the latest advances in biosensor and nanosensor research for detecting and quantitatively identifying reproductive biomarkers.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Chengfang Tang, Fang Tang, Yanna Cai, Minyi Tan, Sichi Liu, Ting Xie, Xiang Jiang, Yonglan Huang
Summary: This study proposes an effective method for screening X-ALD and evaluates the performance of newborn screening for X-ALD in Guangzhou. The LC-MS/MS method can accurately identify X-ALD through analysis of C26:0-LPC.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Lan Liu, Jiamin Wang, Xijing Liu, Jing Wang, Lin Chen, Hongmei Zhu, Jingqun Mai, Ting Hu, Shanling Liu
Summary: The 16p11.2 deletion is a common genetic cause of neurodevelopmental disorders, with prenatal and postnatal presentations including vertebral malformations and language impairment. The majority of deletions are de novo and may be associated with MAPK3 and histidine-associated metabolism.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Sneha Venkatesalu, Shanmugapriya Dilliyappan, Avanthika Satish Kumar, Thirunavukkarasu Palaniyandi, Gomathy Baskar, Maddaly Ravi, Asha Sivaji
Summary: Microfluidics is a science and technology that deals with less sample-to-more precision in vitro analysis. It has wide applications in cancer theranostics, enabling precise diagnosis and personalized treatment.
CLINICA CHIMICA ACTA
(2024)
Review
Medical Laboratory Technology
Yuanqin Zhao, Wei Deng, Zhaoyue Wang, Yanxia Wang, Hongyu Zheng, Kun Zhou, Qian Xu, Le Bai, Huiting Liu, Zhong Ren, Zhisheng Jiang
Summary: The cardiovascular system and the central nervous system exhibit a coordinated developmental process during embryonic development. Congenital heart disease (CHD) is the most common congenital disorder, and neurodevelopmental disorders (NDD) are common complications in CHD patients. Both genetic and non-genetic factors contribute to the co-occurrence of CHD and NDD. Further research should focus on identifying common molecular mechanisms underlying this co-occurrence and promoting the research and treatment of developmental disorders related to the cardiovascular and central nervous systems.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Karol Gostomczyk, Ewelina Lukaszewska, Jedrzej Borowczak, Anita Bator, Marek Zdrenka, Magdalena Bodnar, Lukasz Szylberg
Summary: Flow cytometry improves the detection of epithelial cancer cells in peritoneal and pleural fluids compared to conventional cytology. Due to similar speciflcity and higher sensitivity, flow cytometry offers a promising alternative to cytology for patient screening.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Heping Tian, Genghuan Wang, Qi Zhong, Haihang Zhou
Summary: This study found that the decline of serum ITIH4 concentrations during the early phase after aneurysmal subarachnoid hemorrhage (aSAH) was closely related to the severity and poor prognosis of the disease. Serum ITIH4 may represent a promising prognostic biomarker of aSAH.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Xueting Zhu, Yang Yu, Jun Zhang, Yuxia Zhan, Guanghua Luo, Lu Zheng
Summary: This study successfully established a 2D-PCR method for identifying HLA-B*15:02 through a two-tube reaction. This method can distinguish HLA-B*15:02 from 16 highly homologous HLA-B*15 alleles. Among 1830 samples from the clinical general population, 3 HLA-B*15:02 homozygotes and 84 HLA-B*15:02 heterozygotes were detected.
CLINICA CHIMICA ACTA
(2024)
