Article
Plant Sciences
Leyu Zhang, Duo Lv, Jian Pan, Keyan Zhang, Haifan Wen, Yue Chen, Hui Du, Huanle He, Run Cai, Junsong Pan, Gang Wang
Summary: Trichomes in plants play a crucial role in protection, and a mutant named mict-L130F with abnormal trichome development was identified in cucumber. The gene CsNps controlling the nps trait was mapped to chromosome 3, and further analysis revealed a single nucleotide mutation in a specific gene. Comparative transcriptome analysis highlighted potential target genes related to trichome development.
Article
Plant Sciences
Yaqi Zhang, Junjun Shen, Ezra S. Bartholomew, Mingming Dong, Shuying Chen, Shuai Yin, Xuling Zhai, Zhongxuan Feng, Huazhong Ren, Xingwang Liu
Summary: The TBH gene in cucumber is preferentially expressed in multicellular trichomes and regulates their development, ethylene accumulation, and sex expression by directly binding to the promoters of the CsACS genes. This study provides new insights into the molecular mechanism of CsTBH in cucumber trichome development and sex expression through an ethylene pathway.
Article
Agricultural Engineering
Yanchao Yuan, Qingjia Wang, Boqiang Tong, Dan Liu, Hanzhu Liu, Qingkang Yan, Hua Xin
Summary: The distribution of glandular trichomes (GTs) on the shooting of Lonicera japonica was observed, showing that they were mainly distributed along the veins on the leaf surface and evenly distributed on the stem surface. A new type of GTs with disc-shaped base was identified. Two novel HD-ZIP genes, LjROC3 and LjHD-ZIP1, were found to be up-regulated during GT development. Additionally, LjROC3 was found to increase GT density while LjHD-ZIP1 showed no significant difference.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Agriculture, Multidisciplinary
Hong-yan Chen, Zhu Zhu, Xiao-wen Wang, Yang-yang Li, Dan-ling Hu, Xue-fei Zhang, Lu-qi Jia, Zhi-bo Cui, Xian-chun Sang
Summary: The trichomes of rice leaves are formed by the differentiation and development of epidermal cells. Plant trichomes play a crucial role in stress resistance and protection against direct ultraviolet irradiation. This study identified and characterized a mutant, lhl1 (less hairy leaf 1), which exhibited defective trichome development in rice leaves. The gene responsible for this phenotype, LOC_Os02g25230, was identified and its role in trichome development was verified through RNA interference and overexpression experiments.
JOURNAL OF INTEGRATIVE AGRICULTURE
(2023)
Article
Plant Sciences
Lihui Xie, Tingxiang Yan, Ling Li, Minghui Chen, Danial Hassani, Yongpeng Li, Wei Qin, Hang Liu, Tiantian Chen, Xueqing Fu, Qian Shen, Jocelyn K. C. Rose, Kexuan Tang
Summary: The study identified key transcription factors, AaMYB16 and AaMYB5, that interact with AaHD1 to regulate glandular secretory trichomes (GSTs) initiation in plants. AaMYB16 was found to be a positive regulator while AaMYB5 had an inhibitory effect on GST initiation. The competitive interaction between AaMYB16 and AaMYB5 modulates the binding activity of AaHD1 promoter, providing new insights into the regulatory network controlling GST formation.
Article
Plant Sciences
Zhilang Qiu, Zhuang Wen, Qiandong Hou, Guang Qiao, Kun Yang, Yi Hong, Xiaopeng Wen
Summary: The study revealed that the heavy fruitlet abscission was attributed to malformed pollen structure and low viability. Abscising fruits and carpopodium showed red coloration and aborted embryos, mainly due to low pollen viability and malformation. Transcriptome analysis identified numerous differentially expressed genes, including those related to plant hormone biosynthesis and signal transduction, as well as cell wall remodeling, indicating their crucial roles in regulating the abscission process.
Article
Biochemistry & Molecular Biology
Kai Liu, Xiaolei Han, Zhaolin Liang, Jiadi Yan, Peihua Cong, Caixia Zhang
Summary: This study detected 48 MdHDZ genes in apples and categorized them into three subfamilies. Analysis of chromosomal locations, gene/protein structures, and physiological and biochemical properties revealed the key role of segmental duplications in the expansion of the apple HD-Zip family. MdHDZ genes may be involved in the regulation of apple growth and tolerance to environmental stresses. Additionally, this research suggests the potential involvement of apple HD-Zip genes in the response to fungal infection and adventitious bud regeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Jian Ling, Xiaoxiao Xie, Xingfang Gu, Jianlong Zhao, Xingxing Ping, Yan Li, Yuhong Yang, Zhenchuan Mao, Bingyan Xie
Summary: The African horned cucumber displays high-level resistance to important plant pathogens and has a genome with a large number of resistance-related genes. Its genome shows significant structural variations compared to other species in the Cucurbitaceae family. Originating in Zimbabwe, the cucumber likely underwent similar domestic selection as melon in other southern African regions. These findings will accelerate research on resistance-related gene evolution and improve efforts in cucurbit crop enhancement.
Article
Plant Sciences
Jing Gao, Jiyu Chen, Lingyang Feng, Qi Wang, Shenglan Li, Xianming Tan, Feng Yang, Wenyu Yang
Summary: The HD-Zip III gene family plays a central role in the regulation of vascular development in soybean. A total of twelve HD-Zip III genes were identified in the soybean genome. GmHB15-L-1 may be an essential regulator for the formation or maintenance of soybean vein cambial cells, while GmREV-L-1 and GmHB14-L-2 are correlated with xylem differentiation in soybean leaf veins.
Article
Plant Sciences
Yuehui Tang, Jingrui Peng, Jin Lin, Miaomiao Zhang, Yun Tian, Yaqian Shang, Shuying Chen, Xinxin Bao, Qiyuan Wang
Summary: In this study, a HD-Zip I family gene JcHDZ21 was cloned from physic nut and its expression pattern and function were analyzed. The results showed that JcHDZ21 gene had the highest expression in physic nut seeds and its expression was inhibited by salt stress. Transgenic Arabidopsis plants with ectopic expression of JcHDZ21 showed increased sensitivity to salt stress. This study provides a theoretical basis for the future breeding of salt-tolerant varieties of physic nut using JcHDZ21 gene.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Tingting Ji, Si Ma, Meiting Liang, Xueyun Wang, Lihong Gao, Yongqiang Tian
Summary: This study identified the optimal reference genes for cucumber under M. incognita stress and Pseudomonas treatment, providing potential insights into the interaction between M. incognita or Pseudomonas soil rhizosphere microbe and cucumber.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Genetics & Heredity
Zhige Yang, Mengfei Song, Feng Cheng, Mengru Zhang, Marzieh Davoudi, Jinfeng Chen, Qunfeng Lou
Summary: This study identified a multiple trichomes mutant in cucumber and identified a candidate gene responsible for multicellular trichome development. It was found that the mt gene may regulate epidermal development by influencing plant hormone signaling pathways or participating in several transcription factor pathways. These results provide essential insights into the function of the HD-DDT transcription factor in cucumber trichome development.
Article
Plant Sciences
Yanyan Liu, Shaoyun Dong, Shuang Wei, Weiping Wang, Han Miao, Kailiang Bo, Xingfang Gu, Shengping Zhang
Summary: This study identified a quantitative inherited heat tolerance QTL-qHT1.1 in adult cucumber, with marker HT-1 showing a high accuracy of 97.8% in heat-sensitive plants. Further sequence analysis revealed multiple genes possibly involved in heat tolerance. The study provides valuable markers for heat tolerance breeding in cucumber and lays the foundation for identifying heat-tolerant genes.
Article
Biochemistry & Molecular Biology
Svetlana S. Gorina, Alevtina M. Egorova, Natalia V. Lantsova, Yana Y. Toporkova, Alexander N. Grechkin
Summary: The profiling of endogenous oxylipins in cucumber revealed the abundance of 16-hydroxy-9,12,14-octadecatrienoic acid. The homogenates of cucumber organs yielded 16(S)-hydroperoxide as a predominant product when incubated with a-linolenic acid. The targeted proteomic analysis identified CsLOX3 as the first 16(S)-LOX and w3-LOX that synthesizes 16(S)-HPOT.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Minliang Wu, Jiang Chang, Xiaoqian Han, Jingyuan Shen, Liling Yang, Shourong Hu, Ben-Ben Huang, Huimin Xu, Mengyuan Xu, Shurong Wu, Pengxue Li, Bin Hua, Meina Yang, Zhenbiao Yang, Shuang Wu
Summary: A transcription factor called Woolly controls the fate of different types of trichomes in tomatoes through a dosage-dependent mechanism. This study reveals the developmental switch of trichome formation and the mechanism of fate determination in plants, providing insights for enhancing plant stress resistance and the production of beneficial chemicals.
DEVELOPMENTAL CELL
(2023)
Article
Plant Sciences
Yuhui Wang, Junyi Tan, Zhiming Wu, Kyle VandenLangenberg, Todd C. Wehner, Changlong Wen, Xiangyang Zheng, Ken Owens, Alyson Thornton, Hailey H. Bang, Eric Hoeft, Peter A. G. Kraan, Jos Suelmann, Junsong Pan, Yiqun Weng
Article
Genetics & Heredity
Yue Chen, Gang Wang, Jian Pan, Haifan Wen, Hui Du, Jingxian Sun, Keyan Zhang, Duo Lv, Huanle He, Run Cai, Junsong Pan
Review
Plant Sciences
Yuhui Wang, Kailiang Bo, Xingfang Gu, Junsong Pan, Yuhong Li, Jinfeng Chen, Changlong Wen, Zhonghai Ren, Huazhong Ren, Xuehao Chen, Rebecca Grumet, Yiqun Weng
HORTICULTURE RESEARCH
(2020)
Article
Plant Sciences
Hui Du, Gang Wang, Jian Pan, Yue Chen, Tingting Xiao, Leyu Zhang, Keyan Zhang, Haifan Wen, Liangrong Xiong, Yao Yu, Huanle He, Junsong Pan, Run Cai, Tracy Lawson
JOURNAL OF EXPERIMENTAL BOTANY
(2020)
Article
Genetics & Heredity
Duo Lv, Gang Wang, Liang-Rong Xiong, Jing-Xian Sun, Yue Chen, Chun-Li Guo, Yao Yu, Huan-Le He, Run Cai, Jun-Song Pan
Article
Biochemistry & Molecular Biology
Liangrong Xiong, Hui Du, Keyan Zhang, Duo Lv, Huanle He, Junsong Pan, Run Cai, Gang Wang
Summary: The study identified a yellow leaf mutant, yl2.1, controlled by a recessive nuclear gene encoding a plastid isoform of triose phosphate isomerase (pdTPI). The yl2.1 leaves showed reduced chlorophyll content, abnormal chloroplasts, and impaired photosynthetic efficiency.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Genetics & Heredity
Haifan Wen, Yue Chen, Hui Du, Leyu Zhang, Keyan Zhang, Huanle He, Junsong Pan, Run Cai, Gang Wang
Review
Agronomy
Keyan Zhang, Junsong Pan, Yue Chen, Ying Wei, Hui Du, Jingxian Sun, Duo Lv, Haifan Wen, Huanle He, Gang Wang, Run Cai
Summary: CsSh5.1, controlling hypocotyl elongation in cucumber under high temperature, encodes a xyloglucan galactosyltransferase affecting cell wall strength and extensibility. The research reveals the expression and function of CsSh5.1 in hypocotyls under high temperature conditions.
THEORETICAL AND APPLIED GENETICS
(2021)
Article
Plant Sciences
Jian Pan, Leyu Zhang, Guanqun Chen, Haifan Wen, Yue Chen, Hui Du, Junlong Zhao, Huanle He, Hongli Lian, Huiming Chen, Jianxin Shi, Run Cai, Gang Wang, Junsong Pan
Summary: This study characterized the glabrous mutant mict/csgl1/cstbh in cucumber through metabolomic and transcriptomic analyses, revealing the important role of the mict gene in regulating multicellular trichome development and metabolism. The findings provide insights into transcriptional regulation mechanisms of multicellular trichome development and specific metabolism pathways in cucumber.
HORTICULTURE RESEARCH
(2021)
Article
Plant Sciences
Leyu Zhang, Duo Lv, Jian Pan, Keyan Zhang, Haifan Wen, Yue Chen, Hui Du, Huanle He, Run Cai, Junsong Pan, Gang Wang
Summary: Trichomes in plants play a crucial role in protection, and a mutant named mict-L130F with abnormal trichome development was identified in cucumber. The gene CsNps controlling the nps trait was mapped to chromosome 3, and further analysis revealed a single nucleotide mutation in a specific gene. Comparative transcriptome analysis highlighted potential target genes related to trichome development.
Article
Biochemistry & Molecular Biology
Guanqun Chen, Weizhi He, Xiangxin Guo, Junsong Pan
Summary: This study identified and analyzed the MYB gene family in petunia, revealing different expression patterns in different tissues among genes from various subgroups. Analysis of gene structure and protein motifs supported the results of phylogenetic analysis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Haifan Wen, Jian Pan, Yue Chen, Guanqun Chen, Hui Du, Leyu Zhang, Keyan Zhang, Huanle He, Gang Wang, Run Cai, Junsong Pan
Summary: This study identified the gene CsTFL1 that controls determinate growth in cucumber. It was found that CsTFL1d can partially rescue determinate growth of CsTFL1(del) through induction by long-day and high-temperature signals. Biochemical analysis showed that CsTFL1d interacts with CsNOT2a and functions in a similar regulatory mechanism as CsTFL1.
PLANT CELL AND ENVIRONMENT
(2021)
Review
Agronomy
Yue Chen, Haifan Wen, Jian Pan, Hui Du, Keyan Zhang, Leyu Zhang, Yao Yu, Huanle He, Run Cai, Junsong Pan, Gang Wang
Summary: An unusual flower and tendril (uft) mutant in cucumber was caused by a mutation in the gene encoding an F-box protein, resulting in down-regulation of organ development genes and significantly decreased expression level of CsUFO gene. This study provides new insights into the molecular mechanisms of flower organogenesis in cucumber.
THEORETICAL AND APPLIED GENETICS
(2021)
Article
Plant Sciences
Duo Lv, Gang Wang, Qi Zhang, Yao Yu, Pei-Chao Qin, Jin-An Pang, Jing-Xian Sun, Ke-Yan Zhang, Huan-Le He, Run Cai, Jun-Song Pan
Summary: This study investigated the development of cucumber fruit spines and found that CsTs gene can influence their development. Transcriptome analysis revealed differential gene expression associated with fruit spine development at different stages. Additionally, significant differences were observed between mutant and wild-type spines at stage III. Functional and interaction analyses suggested that CsTs may regulate fruit spine development by influencing cell polarity.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Jian Pan, Haifan Wen, Guanqun Chen, Wen-Hui Lin, Hui Du, Yue Chen, Leyu Zhang, Hongli Lian, Gang Wang, Run Cai, Junsong Pan
Summary: This study reveals the crucial role of a gynoecium-specific gene CsERF31 in sex determination in cucumber, providing insights into how the ethylene signaling pathway promotes female flower development in cucumber.