Article
Biotechnology & Applied Microbiology
Xiaodong Xu, Shanqing Jiang, Longjun Gu, Bin Li, Fang Xu, Changyong Li, Pu Chen
Summary: A novel type of liver organoids can be generated through micropatterning technique, which have consistent morphology, size, and location. These organoids closely recapitulate critical features of human liver development at the fetal stage and allow whole-mount in-situ staining and imaging.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Robert J. Hall, Jonathan O'Loughlin, Charlotte K. Billington, Dhruma Thakker, Ian P. Hall, Ian Sayers
Summary: GPR126 is an adhesion G protein-coupled receptor associated with lung function and COPD, with identified SNPs affecting its function/expression. Activation of GPR126 in airway cells leads to downstream gene/pathway alterations, suggesting a role in airway remodeling. Further research is warranted to elucidate the exact mechanisms involved.
Article
Multidisciplinary Sciences
Max G. Schubert, Daniel B. Goodman, Timothy M. Wannier, Divjot Kaur, Fahim Farzadfard, Timothy K. Lu, Seth L. Shipman, George M. Church
Summary: RLR is a high-throughput method for genetic variant screening, utilizing retrons' reverse transcription activity to produce ssDNA for editing, introducing multiple genomic variants with barcoding for targeted deep sequencing, and suitable for natural variation screening.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Allergy
Stephan A. Ramos, John J. Morton, Prabha Yadav, Brendan Reed, Sheila Alizadeh, Ali H. Shilleh, Loni Perrenoud, James Jaggers, John Kappler, Antonio Jimeno, Holger A. Russ
Summary: This study successfully developed a refined direct differentiation protocol that generates patient-derived thymic epithelial progenitor cells from multiple induced pluripotent stem cell (iPSC) lines, which further differentiate into functional patient-derived thymic epithelial cells. These findings provide important insights for researchers studying human thymus biology.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2022)
Article
Biochemical Research Methods
Qi Wang, Sueanne Chear, Kristof Wing, David Stellon, Minh Thuan Nguyen Tran, Jana Talbot, Alice Pebay, Alex W. Hewitt, Anthony L. Cook
Summary: The combination of induced pluripotent stem cells (iPSCs) and the CRISPR/Cas system provides a powerful tool for disease modeling by enabling programmable gene editing to investigate genetic variants associated with diseases affecting multiple organs. In this study, an optimized protocol for using CRISPR/Cas ribonucleoproteins to edit disease-causing gene variants in human iPSCs is described, offering an important method for disease research.
Review
Cardiac & Cardiovascular Systems
Mengcheng Shen, Thomas Quertermous, Michael P. Fischbein, Joseph C. Wu
Summary: The developmental origin of vascular smooth muscle cells plays a crucial role in regional susceptibility and resistance to vascular diseases. Patient induced pluripotent stem cell-derived VSMCs are valuable for research and therapeutic applications, but the heterogeneity in developmental origins poses challenges.
CIRCULATION RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Chih-Hsien Wu, Hsuan-Hwai Lin, Yi-Ying Wu, Yi-Lin Chiu, Li-Yen Huang, Cheng-Chung Cheng, Chung-Chi Yang, Tsung-Neng Tsai
Summary: Coronary artery disease is a major global issue. Patients with coronary artery disease often develop peptic ulcers when receiving aspirin treatment, making panendoscopy with biopsy necessary for diagnosis and treatment. A protocol for generating iPSCs from gastric fibroblasts via biopsy was established, with successful differentiation into functional cardiomyocytes demonstrated. This study represents the first in vitro generation of iPSCs from gastric fibroblasts.
Article
Multidisciplinary Sciences
Yin-Yu Lam, Chun-Ho Chan, Lin Geng, Nicodemus Wong, Wendy Keung, Yiu-Fai Cheung
Summary: Cardiomyocytes can be derived from hiPSC lines, but the efficacy varies. By performing single cell transcriptomics, researchers identified cardiac and extra-cardiac lineages during differentiation. They found APLNR as a surface marker for in vitro cardiac progenitors, and using this marker, they isolated and differentiated cardiac progenitors from multiple hiPSC lines. This study has potential applications in studying cardiac development, disease modeling, and cardiac regeneration.
Article
Biochemistry & Molecular Biology
Xuehui Fan, Lukas Cyganek, Katja Nitschke, Stefanie Uhlig, Philipp Nuhn, Karen Bieback, Daniel Duerschmied, Ibrahim El-Battrawy, Xiaobo Zhou, Ibrahim Akin
Summary: This study compared the electrophysiological and functional properties of endothelial cells derived from human induced pluripotent stem cells with primary human cardiac microvascular endothelial cells. The results demonstrated that the two types of endothelial cells were similar in terms of ion channel function, membrane receptor signaling, and physiological cell functions, although some differences were observed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Engineering, Biomedical
Yanjie Zhang, Tingyun Lei, Chenqi Tang, Yangwu Chen, Youguo Liao, Wei Ju, Hong Zhang, Bo Zhou, Renjie Liang, Tao Zhang, Chunmei Fan, Xiaoyi Chen, Yanyan Zhao, Yuanhao Xie, Jinchun Ye, Boon Chin Heng, Xiao Chen, Yi Hong, Weiliang Shen, Zi Yin
Summary: By using small molecules in an ex vivo culture system, this study established an effective stepwise culture strategy for TSPCs application, leading to enhanced expression of tendon-related genes and proteins. Combining small molecule-based culture system with 3D printing technology enabled the construction of engineered tendon grafts, which showed improved ability in promoting functional tendon repair and regeneration in vivo and in situ.
Article
Cell Biology
Jana Krueger, Markus Breunig, Lino Pascal Pasquini, Mareen Morawe, Alexander Gross, Frank Arnold, Ronan Russell, Thomas Seufferlein, Ninel Azoitei, Hans A. Kestler, Cecile Julier, Sandra Heller, Meike Hohwieler, Alexander Kleger
Summary: In this study, human pluripotent stem cells were used to identify novel genes involved in early endodermal development. The findings provide important insights into human development and disease models.
Review
Cell Biology
Mourad A. M. Aboul-Soud, Alhusain J. Alzahrani, Amer Mahmoud
Summary: The discovery of induced pluripotent stem cells (iPSCs) has revolutionized biological research, providing valuable models for drug screening and disease modeling in regenerative medicine. iPSCs have the potential to circumvent ethical concerns surrounding embryonic stem cells and offer a promising avenue for clinical translation research.
Article
Cell Biology
Ying Zhang, Jun Wei, Jiani Cao, Kehua Zhang, Yaojin Peng, Hongkui Deng, Jiuhong Kang, Guangjin Pan, Yong Zhang, Boqiang Fu, Shijun Hu, Jie Na, Yan Liu, Lei Wang, Lingmin Liang, Huanxin Zhu, Yu Zhang, Zi-Bing Jin, Jie Hao, Aijin Ma, Tongbiao Zhao, Junying Yu
Summary: "Requirements for Human-Induced Pluripotent Stem Cells" is the first set of guidelines in China on human-induced pluripotent stem cells. It provides detailed technical requirements and aims to promote international standardization.
CELL PROLIFERATION
(2022)
Article
Cell Biology
Hye Ryoun Jang, Hyung Joon Cho, Yang Zhou, Ning-Yi Shao, Kyungho Lee, Hoai Huong Thi Le, Junseok Jeon, Jung Eun Lee, Wooseong Huh, Sang-Ging Ong, Won Hee Lee, Yoon-Goo Kim
Summary: The study aimed to develop a novel model of uremic vasculopathy and investigate the effects of uremic toxins on iPSC-derived endothelial cells. The findings demonstrated that simplified uremic toxin mixtures can simulate uremic vasculopathy effectively and CKD patient-specific iPSC-ECs may recapitulate susceptibility to uremic vasculopathy.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Immunology
Iki Sonn, Fumiko Honda-Ozaki, Sho Yoshimatsu, Satoru Morimoto, Hirotaka Watanabe, Hideyuki Okano
Summary: This study established a new method to generate microglia from human induced pluripotent stem cells (hiPSCs) that resulted in a greater number of hiPSC-derived microglia with high expression of microglia-specific markers. Whole-transcriptome analysis showed that these microglia were similar to human primary microglia. In addition, their specific physiological functions were confirmed through indices of lipopolysaccharide responsiveness, phagocytotic ability, and inflammasome formation. Co-culturing these microglia with mouse primary neurons demonstrated their ability to regulate neuronal activity and maturation.
INFLAMMATION AND REGENERATION
(2022)
Article
Cardiac & Cardiovascular Systems
Indroneal Banerjee, Katrina Carrion, Ricardo Serrano, Jeffrey Dyo, Roman Sasik, Sean Lund, Erik Willems, Seema Aceves, Rudolph Meili, Mark Mercola, Ju Chen, Alexander Zambon, Gary Hardiman, Taylor A. Doherty, Stephan Lange, Juan C. del Alamo, Vishal Nigam
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
(2015)
Article
Cell & Tissue Engineering
Joaquim Cabral-Teixeira, Almudena Martinez-Fernandez, Wenqing Cai, Andre Terzic, Mark Mercola, Erik Willems
STEM CELL RESEARCH
(2015)
Review
Biochemistry & Molecular Biology
Daniel Laengle, Jonas Halver, Bernd Rathmer, Erik Willems, Dennis Schade
ACS CHEMICAL BIOLOGY
(2014)
Editorial Material
Cardiac & Cardiovascular Systems
Erik Willems, Mark Mercola
CIRCULATION RESEARCH
(2013)
Editorial Material
Cardiac & Cardiovascular Systems
Erik Willems, Mark Mercola
CIRCULATION RESEARCH
(2014)
Article
Cell Biology
Wenqing Cai, Sonia Albini, Ke Wei, Erik Willems, Rosa M. Guzzo, Masanao Tsuda, Lorenzo Giordani, Sean Spiering, Leo Kurian, Gene W. Yeo, Pier Lorenzo Puri, Mark Mercola
GENES & DEVELOPMENT
(2013)
Article
Chemistry, Medicinal
Marion Lanier, Dennis Schade, Erik Willems, Masanao Tsuda, Sean Spiering, Jaroslaw Kalisiak, Mark Mercola, John R. Cashman
JOURNAL OF MEDICINAL CHEMISTRY
(2012)
Article
Biochemical Research Methods
Stephen A. Bustin, Vladimir Benes, Jeremy Garson, Jan Hellemans, Jim Huggett, Mikael Kubista, Reinhold Mueller, Tania Nolan, Michael W. Pfaffl, Gregory Shipley, Carl T. Wittwer, Peter Schjerling, Philip J. Day, Monica Abreu, Begona Aguado, Jean-Francois Beaulieu, Anneleen Beckers, Sara Bogaert, John A. Browne, Fernando Carrasco-Ramiro, Liesbeth Ceelen, Kate Ciborowski, Pieter Cornillie, Stephanie Coulon, Ann Cuypers, Sara De Brouwer, Leentje De Ceuninck, Jurgen De Craene, Helene De Naeyer, Ward De Spiegelaere, Kato Deckers, Annelies Dheedene, Kaat Durinck, Margarida Ferreira-Teixeira, Annelies Fieuw, Jack M. Gallup, Sandra Gonzalo-Flores, Karen Goossens, Femke Heindryckx, Elizabeth Herring, Hans Hoenicka, Laura Icardi, Rolf Jaggi, Farzad Javad, Michael Karampelias, Frederick Kibenge, Molly Kibenge, Candy Kumps, Irina Lambertz, Tim Lammens, Amelia Markey, Peter Messiaen, Evelien Mets, Sofia Morais, Alberto Mudarra-Rubio, Justine Nakiwala, Hilde Nelis, Pal A. Olsvik, Claudina Perez-Novo, Michelle Plusquin, Tony Remans, Ali Rihani, Paulo Rodrigues-Santos, Pieter Rondou, Rebecca Sanders, Katharina Schmidt-Bleek, Kerstin Skovgaard, Karen Smeets, Laura Tabera, Stefan Toegel, Tim Van Acker, Wim Van den Broeck, Joni Van der Meulen, Mireille Van Gele, Gert Van Peer, Mario Van Poucke, Nadine Van Roy, Sarah Vergult, Joris Wauman, Marina Tshuikina-Wiklander, Erik Willems, Sara Zaccara, Fjoralba Zeka, Jo Vandesompele
Article
Medicine, Research & Experimental
Agustin Rojas-Munoz, Mano R. Maurya, Frederick Lo, Erik Willems
WILEY INTERDISCIPLINARY REVIEWS-SYSTEMS BIOLOGY AND MEDICINE
(2014)
Article
Multidisciplinary Sciences
Vipul Bhargava, Pang Ko, Erik Willems, Mark Mercola, Shankar Subramaniam
SCIENTIFIC REPORTS
(2013)
Article
Multidisciplinary Sciences
BanuPriya Sridharan, Christopher Hubbs, Nerea Llamosas, Murat Kilinc, Fakhar U. Singhera, Erik Willems, David R. Piper, Louis Scampavia, Gavin Rumbaugh, Timothy P. Spicer
SCIENTIFIC REPORTS
(2019)
Article
Biology
Rachel A. Battaglia, Adriana S. Beltran, Samed Delic, Raluca Dumitru, Jasmine A. Robinson, Parijat Kabiraj, Laura E. Herring, Victoria J. Madden, Namritha Ravinder, Erik Willems, Rhonda A. Newman, Roy A. Quinlan, James E. Goldman, Ming-Der Perng, Masaki Inagaki, Natasha T. Snider
Article
Pharmacology & Pharmacy
Jonas Halver, Kristin Wenzel, Jandirk Sendker, Carmen Carrillo Garcia, Clemens A. J. Erdelmeier, Erik Willems, Mark Mercola, Nico Symma, Stephanie Koenemann, Egon Koch, Andreas Hensel, Dennis Schade
FRONTIERS IN PHARMACOLOGY
(2019)
Article
Neurosciences
Nerea Llamosas, Vineet Arora, Ridhima Vij, Murat Kilinc, Lukasz Bijoch, Camilo Rojas, Adrian Reich, BanuPriya Sridharan, Erik Willems, David R. Piper, Louis Scampavia, Timothy P. Spicer, Courtney A. Miller, J. Lloyd Holder, Gavin Rumbaugh
JOURNAL OF NEUROSCIENCE
(2020)
