期刊
CIRCULATION RESEARCH
卷 111, 期 1, 页码 28-36出版社
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1161/CIRCRESAHA.112.265652
关键词
calcium-induced calcium release; cardiac muscle; ryanodine receptor; sarcoplasmic reticulum; spark
资金
- National Institutes of Health [R01-HL057832, R01-AR054098]
- American Heart Association
Rationale: In cardiac muscle, Ca2+-induced Ca2+ release (CICR) from the sarcoplasmic reticulum (SR) is mediated by ryanodine receptor (RyR) Ca2+ release channels. The inherent positive feedback of CICR is normally well-controlled. Understanding this control mechanism is a priority because its malfunction has life-threatening consequences. Objective: We show that CICR local control is governed by SR Ca2+ load, largely because load determines the single RyR current amplitude that drives inter-RyR CICR. Methods and Results: We differentially manipulated single RyR Ca2+ flux amplitude and SR Ca2+ load in permeabilized ventricular myocytes as an endogenous cell biology model of the heart. Large RyR-permeable organic cations were used to interfere with Ca2+ conductance through the open RyR pore. Single-channel studies show this attenuates current amplitude without altering other aspects of RyR function. In cells, the same experimental maneuver increased resting SR Ca2+ load. Despite the increased load, Ca2+ spark (inter-RyR CICR events) frequency decreased and sparks terminated earlier. Conclusions: Spark local control follows single RyR current amplitude, not simply SR Ca2+ load. Spark frequency increases with load because spontaneous RyR openings at high loads produce larger currents (ie, a larger CICR trigger signal). Sparks terminate when load falls to the point at which single RyR current amplitude is no longer sufficient to sustain inter-RyR CICR. Thus, RyRs that spontaneously close no longer reopen and local Ca2+ release ends. (Circ Res. 2012;111:28-36.)
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