Review
Plant Sciences
Luciano Di Fino, Andres Arruebarrena Di Palma, Enzo A. Perk, Carlos Garcia-Mata, Francisco J. Schopfer, Ana M. Laxalt
Summary: Nitro fatty acids serve as important signaling molecules in biotic and abiotic stress, growth, and development, but the mechanisms of action are controversial. Two main synthesis mechanisms involve nitric oxide release and thiol modification.
Article
Cardiac & Cardiovascular Systems
Jae Min Cho, Seul-Ki Park, Oh Sung Kwon, D. Taylor La Salle, James Cerbie, Caitlin C. Fermoyle, David Morgan, Ashley Nelson, Amber Bledsoe, Leena P. Bharath, Megan Tandar, Satya P. Kunapuli, Russell S. Richardson, Pon Velayutham Anandh Babu, Sohom Mookherjee, Bellamkonda K. Kishore, Fei Wang, Tianxin Yang, Sihem Boudina, Joel D. Trinity, J. David Symons
Summary: This study explores the importance of endothelial cell autophagy in maintaining vascular homeostasis and its role in nitric oxide generation. The findings suggest that autophagy is necessary for glycolysis-dependent purinergic signaling to eNOS, and impaired autophagy leads to arterial dysfunction. The activation of P2Y(1)-Rs improves arterial function in cases of compromised autophagy.
CARDIOVASCULAR RESEARCH
(2022)
Article
Cardiac & Cardiovascular Systems
Felix Sebastian Nettersheim, Julian Lemties, Simon Braumann, Simon Geissen, Senai Bokredenghel, Richard Nies, Alexander Hof, Holger Winkels, Bruce A. Freeman, Anna Klinke, Volker Rudolph, Stephan Baldus, Dennis Mehrkens, Martin Mollenhauer, Matti Adam
Summary: This study found that Nitro-oleic acid can attenuate the progression of aortic dilation in Marfan syndrome by modulating multiple disease-mediating pathways.
CARDIOVASCULAR RESEARCH
(2022)
Article
Plant Sciences
Pei Long, Yong Li, Qing Wen, Maohua Huang, Songtao Li, Yuning Lin, Xiaojun Huang, Minfeng Chen, Jie Ouyang, Yunlin Ao, Qi Qi, Haipeng Zhang, Wencai Ye, Guohua Cheng, Xiaoqi Zhang, Dongmei Zhang
Summary: The study shows that OTNA relaxes pulmonary arteries by antagonizing AhR, providing a potential therapeutic mechanism for PAH.
Article
Nutrition & Dietetics
Elena Vega-Martin, Marta Gil-Ortega, Raquel Gonzalez-Blazquez, Sara Benedito, Jesus Fernandez-Felipe, Mariano Ruiz-Gayo, Nuria del Olmo, Julie A. Chowen, Laura M. Frago, Beatriz Somoza, Maria S. Fernandez-Alfonso
Summary: The study found that high-fat diets enriched with saturated fatty acids have a negative impact on vascular function and structure, leading to endothelial dysfunction and arterial stiffness.
Article
Nutrition & Dietetics
Zbigniew Jost, Maja Tomczyk, Maciej Chroboczek, Philip C. Calder, Helena L. Fisk, Katarzyna Przewlocka, Jedrzej Antosiewicz
Summary: The effect of omega-3 fatty acid supplementation on the metabolism of amino acids required for NO synthesis, as well as its impact on running economy in runners, is not fully understood. In this 12-week study, male amateur endurance runners were divided into two groups: one supplemented with omega-3 fatty acids and the other with medium-chain triglycerides. The results showed that omega-3 supplementation increased the levels of certain amino acids and the ratio of L-arginine to asymmetric dimethylarginine, but did not improve running economy.
Article
Biochemistry & Molecular Biology
Tanmoy Kumar Dey, Puja Bose, Sangita Paul, Bipul Chandra Karmakar, Rudra Narayan Saha, Animesh Gope, Hemanta Koley, Amit Ghosh, Shanta Dutta, Pubali Dhar, Asish Kumar Mukhopadhyay
Summary: Fish oil-in-water nanoemulsion can protect the intestinal epithelial barrier against invasive infection of non-typhoidal Salmonella and reduce inflammation.
Article
Pharmacology & Pharmacy
Esra Akcabag, Zeliha Bayram, Ikbal Ozen Kucukcetin, Gulbahar Uzun, Sebahat Ozdem, Sadi S. Ozdem
Summary: Visfatin can inhibit the endothelium-dependent relaxation responses of isolated rat small resistance arteries to acetylcholine, likely mediated by oxygen free radicals and a reduction in nitric oxide bioavailability. High levels of visfatin in systemic and/or local environments due to pathologies like obesity may contribute to vascular dysfunctions.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2021)
Article
Engineering, Multidisciplinary
Sohail Nadeem, Salman Akhtar, Anber Saleem, Nevzat Akkurt, Hassan Ali Ghazwani, Sayed M. Eldin
Summary: This research investigates the analysis of arterial blood flow through different stenotic regions. Four types of stenotic regions, including triangular, trapezoidal, overlapping (w-shape), and composite formations, are studied for the first time in numerical analysis. An elliptical cross-sectional artery is modeled using a cartesian coordinate system, and the governing partial differential equations are numerically solved with the Open-FOAM CFD software. The results show a high flow profile with minor disruptions near the origin of stenotic sections, and high pressure values near the sharp corners of stenotic regions. Expected irregularities in the flow profile are observed, typical of real-life stenosed artery blood flow problems.
ALEXANDRIA ENGINEERING JOURNAL
(2023)
Article
Environmental Sciences
Ashraf Albrakati
Summary: Caveolae serve as organizing centers for cellular signal transduction in blood vessels. Disruption of caveolae using MBCD affects the contractility of rat carotid arteries by altering the function of maxi-K channels and inhibiting the release of NO from endothelial cells.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Cheng Zhong, Minze Xu, Senguel Boral, Holger Summer, Falk-Bach Lichtenberger, Cem Erdogan, Maik Gollasch, Stefan Golz, Pontus B. Persson, Johanna Schleifenbaum, Andreas Patzak, Pratik H. Khedkar
Summary: Aging affects the downstream signaling of the endothelial nitric oxide system in vascular smooth muscle, leading to compromised vasorelaxation even in the absence of histopathological alterations. The reduced expression of sGC and PDE5 may contribute to the impaired vasorelaxation in aged vessels.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Physiology
Weipeng Hu, Shan Jiang, Yixin Liao, Jinhong Li, Fang Dong, Jie Guo, Xiaohua Wang, Lingyan Fei, Yu Cui, Xiaoqiu Ren, Nan Xu, Liang Zhao, Limeng Chen, Yali Zheng, Lingli Li, Andreas Patzak, Pontus B. Persson, Zhihua Zheng, En Yin Lai
Summary: The study found that high phosphate diet can impair endothelial function in arteries and kidneys, reduce GFR, and decrease blood pressure response to acetylcholine. Additionally, high phosphate levels also increase mitochondrial oxidative stress and decrease expression of mitochondrial-related antioxidant genes.
Article
Peripheral Vascular Disease
Estefano Pinilla, Simon Comerma-Steffensen, Judit Prat-Duran, Luis Rivera, Vladimir V. Matchkov, Niels Henrik Buus, Ulf Simonsen
Summary: Pharmacological modulation of TG2 to the closed conformation age-dependently lowers blood pressure and enhances endothelium-dependent vasorelaxation by opening potassium channels. In older rats, LDN 27219 effectively improves acetylcholine-induced relaxation and decreases blood pressure compared to younger rats, indicating potential for treating age-related vascular dysfunction.
Article
Plant Sciences
Yethreb Mahjoubi, Touhami Rzigui, Oussama Kharbech, Salma Nait Mohamed, Leila Abaza, Abdelilah Chaoui, Issam Nouairi, Wahbi Djebali
Summary: Nitric oxide can alleviate manganese toxicity in plants by enhancing growth and restoring leaf lipid content, which may be attributed to NO/Mn cross-talk.
Article
Physiology
Eric Leslie, Vanessa Lopez, Nana A. O. Anti, Rafael Alvarez, Isaac Kafeero, Donald G. Welsh, Monica Romero, Shawn Kaushal, Catherine M. Johnson, Remy Bosviel, Ivana Blazenovic, Rui Song, Alex Brito, Michael R. La Frano, Lubo Zhang, John W. Newman, Oliver Fiehn, Sean M. Wilson
Summary: The research demonstrates that long-term hypoxia in fetuses leads to metabolic reprogramming and phenotypic transformations in the pulmonary arteries of fetal sheep, potentially contributing to the development of persistent pulmonary hypertension.
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
(2021)
Article
Cardiac & Cardiovascular Systems
Yang Zhao, Ziyi Chang, Guizhen Zhao, Haocheng Lu, Wenhao Xiong, Wenying Liang, Huilun Wang, Luis Villacorta, Minerva T. Garcia-Barrio, Tianqing Zhu, Yanhong Guo, Yanbo Fan, Lin Chang, Francisco J. Schopfer, Bruce A. Freeman, Jifeng Zhang, Y. Eugene Chen
Summary: Administration of NO2-OA significantly reduces the incidence of AAA and the diameter of the supra-renal aorta in a mouse model. It also decreases extracellular matrix degradation, inflammatory cytokine levels, and leucocyte/macrophage infiltration in the vasculature. The protective effect of NO2-OA is partially mediated through activation of PPAR gamma and inhibition of macrophage prostaglandin E2-induced EP4 cAMP signaling.
CARDIOVASCULAR DRUGS AND THERAPY
(2021)
Article
Plant Sciences
Luciano M. Di Fino, Ignacio Cerrudo, Sonia R. Salvatore, Francisco J. Schopfer, Carlos Garcia-Mata, Ana M. Laxalt
FRONTIERS IN PLANT SCIENCE
(2020)
Article
Biochemistry & Molecular Biology
Sonia R. Salvatore, Pascal Rowart, Francisco J. Schopfer
Summary: Studies have found that isomers of two fatty acids nitrated were discovered in human urine, with the main group corresponding to nitrated conjugated linolenic acid (NO2-CLnA). Previously reported nitrated omega-3's, oleic acid, arachidonic acid and alpha- and gamma-linolenic acid were unable to be confirmed, suggesting the need for a re-evaluation of their biological formation and presence in humans. Metabolite analysis of NO2-CLnA in human urine revealed their relative abundance and possible role in cell signaling through electrophilic properties and Michael addition reactions.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Matias M. Vazquez, Maria Gutierrez, Sonia R. Salvatore, Marcelo Puiatti, Virginia Actis Dato, Gustavo A. Chiabrando, Bruce A. Freeman, Francisco J. Schopfer, Gustavo Bonacci
Article
Biochemistry & Molecular Biology
Lucia Turell, Martina Steglich, Maria J. Torres, Matias Deambrosi, Laura Antmann, Cristina M. Furdui, Francisco J. Schopfer, Beatriz Alvarez
Summary: This study confirmed the formation process of HSA-SOH and its further oxidation to sulfinic acid and sulfonic acid in the presence of hydrogen peroxide. The decay pathways of HSA-SOH were investigated, revealing its reaction with thiols to form mixed disulfides.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Marco Fazzari, Steven R. Woodcock, Pascal Rowart, Karina Ricart, Jack R. Lancaster Jr, Rakesh Patel, Dario A. Vitturi, Bruce A. Freeman, Francisco J. Schopfer
Summary: This study identified nitro-nitrate ester-containing lipids as intermediates in unsaturated fatty acid nitration reactions in rat stomach, leading to the production of anti-inflammatory and tissue-protective signaling mediators. These observations provide new insight into the mechanisms of unsaturated fatty acid nitration and expand the scope of endogenous products resulting from nitrogen oxides metabolic reactions.
Article
Chemistry, Organic
Steven R. Woodcock, Sonia R. Salvatore, Bruce A. Freeman, Francisco J. Schopfer
Summary: This study reports synthetic methods for obtaining three main biological nitrated fatty acids from commercially available starting materials, for biological evaluation.
TETRAHEDRON LETTERS
(2021)
Article
Biochemistry & Molecular Biology
Peng Wang, Meaghan E. Killeen, Tina L. Sumpter, Laura K. Ferris, Louis D. Falo, Bruce A. Freeman, Francisco J. Schopfer, Alicia R. Mathers
Summary: Electrophilic nitro-fatty acids have potent anti-inflammatory effects in murine models of chronic inflammatory diseases and show therapeutic potential for psoriasiform dermatitis. Oral treatment with nitro oleic acid can prevent and treat psoriasiform inflammation, downregulate inflammatory cytokine production, and inhibit keratinocyte proliferation through NF-kappa B and STAT3 pathways.
Article
Multidisciplinary Sciences
Dana Al Rijjal, Ying Liu, Mi Lai, Youchen Song, Zahra Danaei, Anne Wu, Haneesha Mohan, Li Wei, Francisco J. Schopfer, Feihan F. Dai, Michael B. Wheeler
Summary: In a study conducted on mice, Vascepa was found to have protective effects on diet-induced insulin resistance and glucose intolerance, as well as improving beta cell function and expression of hepatic fatty acid oxidation genes.
Review
Plant Sciences
Luciano Di Fino, Andres Arruebarrena Di Palma, Enzo A. Perk, Carlos Garcia-Mata, Francisco J. Schopfer, Ana M. Laxalt
Summary: Nitro fatty acids serve as important signaling molecules in biotic and abiotic stress, growth, and development, but the mechanisms of action are controversial. Two main synthesis mechanisms involve nitric oxide release and thiol modification.
Article
Biochemistry & Molecular Biology
Shuai Yuan, Scott A. Hahn, Megan P. Miller, Subramaniam Sanker, Michael J. Calderon, Mara Sullivan, Atinuke M. Dosunmu-Ogunbi, Marco Fazzari, Yao Li, Michael Reynolds, Katherine C. Wood, Claudette M. St Croix, Donna Stolz, Eugenia Cifuentes-Pagano, Placido Navas, Sruti Shiva, Francisco J. Schopfer, Patrick J. Pagano, Adam C. Straub
Summary: The study demonstrates that CYB5R3 assists in NOX4-dependent H2O2 generation via CoQ, mitigating endothelial inflammatory activation. Knockout of CYB5R3 led to endothelial dysfunction in mice and enhanced VCAM-1 expression, which was alleviated by silencing NOX4. Overall, these findings suggest a regulatory role of CYB5R3 in endothelial inflammation through interaction with NOX4 and CoQ.
Article
Biochemistry & Molecular Biology
Fabliha A. Chowdhury, Nicole Colussi, Malini Sharma, Katherine C. Wood, Julia Z. Xu, Bruce A. Freeman, Francisco J. Schopfer, Adam C. Straub
Summary: Sickle cell disease (SCD) is a hereditary hematological disease with high morbidity and mortality rates worldwide. This article evaluates the potential of small molecule nitroalkenes as a therapy for SCD and highlights their protective effects against end organ damage.
Meeting Abstract
Hematology
Heidi M. Schmidt, Scott Hahn, Gowtham K. Annarapu, Mara Carreno, Francisco Schopfer, Sruti Shiva, Dario A. Vitturi, Eric Kelley, Adam C. Straub
Meeting Abstract
Immunology
Peng Wang, Meaghan Killeen, Bruce Freeman, Louis D. Falo, Francisco Schopfer, Alicia R. Mathers
JOURNAL OF IMMUNOLOGY
(2020)
Meeting Abstract
Biochemistry & Molecular Biology
Heidi Schmidt, Scott Hahn, Gowtham Annarapu, Mara Carreno, Francisco Schopfer, Dario Vitturi, Sruti Shiva, Eric Kelley, Adam Straub
FREE RADICAL BIOLOGY AND MEDICINE
(2020)