期刊
CIRCULATION
卷 128, 期 7, 页码 729-736出版社
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1161/CIRCULATIONAHA.113.001371
关键词
macrophages; magnetic resonance imaging; therapeutic thrombolysis; venous thrombosis
资金
- British Heart Foundation [PG/08/039/24436]
- Wellcome Trest [WT090252MA]
- Chilean Agency for Research in Science and Technology (CONICYT)
- British Heart Foundation [FS/11/37/28819, RG/12/1/29262] Funding Source: researchfish
- Medical Research Council [G1000327] Funding Source: researchfish
- National Institute for Health Research [CL-2011-17-006, ACF-2008-17-021] Funding Source: researchfish
- MRC [G1000327] Funding Source: UKRI
Background-The magnetic resonance longitudinal relaxation time (T-1) changes with thrombus age in humans. In this study, we investigate the possible mechanisms that give rise to the T-1 signal in venous thrombi and whether changes in T-1 relaxation time are informative of the susceptibility to lysis. Methods and Results-Venous thrombosis was induced in the vena cava of BALB/C mice, and temporal changes in T-1 relaxation time correlated with thrombus composition. The mean T-1 relaxation time of thrombus was shortest at 7days following thrombus induction and returned to that of blood as the thrombus resolved. T-1 relaxation time was related to thrombus methemoglobin formation and further processing. Studies in inducible nitric oxide synthase (iNOS(-/-))-deficient mice revealed that inducible nitric oxide synthase mediates oxidation of erythrocyte lysis-derived iron to paramagnetic Fe3+, which causes thrombus T-1 relaxation time shortening. Studies using chemokine receptor-2-deficient mice (Ccr2(-/-)) revealed that the return of the T-1 signal to that of blood is regulated by removal of Fe3+ by macrophages that accumulate in the thrombus during its resolution. Quantification of T-1 relaxation time was a good predictor of successful thrombolysis with a cutoff point of <747 ms having a sensitivity and specificity to predict successful lysis of 83% and 94%, respectively. Conclusions-The source of the T-1 signal in the thrombus results from the oxidation of iron (released from the lysis of trapped erythrocytes in the thrombus) to its paramagnetic Fe3+ form. Quantification of T-1 relaxation time appears to be a good predictor of the success of thrombolysis.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据