期刊
JOURNAL OF INORGANIC BIOCHEMISTRY
卷 149, 期 -, 页码 1-5出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.jinorgbio.2015.03.012
关键词
Protein phosphatase-1; Inhibitor-2; TXRF; Iron; Zinc
资金
- Fund for Scientific Research-Flanders [G.0482.12]
- Prime Minister's office [IAP7/13]
Protein phosphatase-1 (PP1) is a major protein Ser/Thr phosphatase in eukaryotic cells. Its activity depends on two metal ions in the catalytic site, which were identified as manganese in the bacterially expressed phosphatase. However, the identity of the metal ions in native PP1 is unknown. In this study, total reflection X-ray fluorescence (TXRF) was used to detect iron and zinc in PP1 that was purified from rabbit skeletal muscle. Metal exchange experiments confirmed that the distinct substrate specificity of recombinant and native PP1 is determined by the nature of their associated metals. We also found that the iron level associated with native PP1 is decreased by incubation with inhibitor-2, consistent with a function of inhibitor-2 as a PP1 chaperone. (C) 2015 Elsevier Inc. All rights reserved
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