Review
Cell Biology
William Khoury, Ryan Trus, Xingyu Chen, Leili Baghaie, Mira Clark, Myron R. Szewczuk, Mohammad El-Diasty
Summary: Angiogenesis is the process of developing new blood vessels for delivering oxygen and nutrients to growing tissues. Pentoxifylline (PTX) has been used to manage vascular disorders, and its effects on angiogenesis have been studied. While some studies suggest that PTX has an antiangiogenic effect, others show it may have a proangiogenic effect or no effect at all. The mechanisms and clinical implications of PTX in angiogenesis are still unclear.
Article
Reproductive Biology
Dong-Dong Gao, Chong-Feng Lan, Xiao-Nian Cao, Lei Chen, Tian-Lun Lei, Lei Peng, Jia-Wen Xu, Zhuo-Er Qiu, Long-Long Wang, Qing Sun, Zi-Yang Huang, Yun-Xin Zhu, Wen-Liang Zhou, Yi-Lin Zhang
Summary: In this study, it was found that GPER is expressed at the acrosome and mid-flagellum of mouse sperm. Activation of GPER triggers an increase in intracellular Ca2+ concentration in mouse sperm through PLC-dependent Ca2+ mobilization and CatSper-mediated Ca2+ influx, which promotes the acrosome reaction of mouse sperm.
BIOLOGY OF REPRODUCTION
(2022)
Article
Engineering, Environmental
Liping Lu, Lilai Shen, Shixuan Cui, Yizhou Huang, Yuchen Gao, Xiaoming Zhu, Shaoyong Lu, Chunlong Zhang, Shulin Zhuang
Summary: Bisphenols at environmentally-relevant concentrations can induce abnormal angiogenesis through disruption of different nuclear receptor-mediated phosphatidylinositol 3-kinase pathways. Bisphenol A (BPA) and bisphenol S (BPS) promote angiogenesis, while tetrabromobisphenol S (TBBPS) inhibits angiogenesis. The different effects are attributed to their distinct interactions with phosphatidylinositol 3-kinase (PI3K) and thyroid receptor (TR) or estrogen receptor (ER).
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Review
Cell Biology
Haoran Jiang, Daniella Galtes, Jialu Wang, Howard A. Rockman
Summary: This review explores the signaling pathways, dynamic structures, and physiological relevance of the three most important GPCR signaling effectors in the cardiovascular system: heterotrimeric G proteins, GPCR kinases (GRKs), and 8-arrestins. It summarizes their prominent roles in GPCR pharmacology before transitioning into less well-explored areas. The application of new technologies has contributed to an increasing understanding of GPCR structure and downstream effectors.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2022)
Article
Cell Biology
Vadim Le Joncour, Pierre-Olivier Guichet, Kleouforo-Paul Dembele, Alexandre Mutel, Daniele Campisi, Nicolas Perzo, Laurence Desrues, Romain Modzelewski, Pierre-Olivier Couraud, Jerome Honnorat, Francois-Xavier Ferracci, Florent Marguet, Annie Laquerriere, Pierre Vera, Pierre Bohn, Olivier Langlois, Fabrice Morin, Pierrick Gandolfo, Helene Castel
Summary: The study demonstrated that urotensin II induces aggressive behavior in GBM, leading to necrosis and angiogenesis through integrin activation, which can be targeted by UT biased ligands/antagonists.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Li-Hua Zhao, Jingyu Lin, Su-Yu Ji, X. Edward Zhou, Chunyou Mao, Dan-Dan Shen, Xinheng He, Peng Xiao, Jinpeng Sun, Karsten Melcher, Yan Zhang, Xiao Yu, H. Eric Xu
Summary: This study presents the structures of CRF2R bound to UCN1 and coupled to G proteins G(11) and G(o), and compares them with the structure of CRF2R bound to G(s), uncovering the structural differences that determine the selective coupling of G protein subtypes by CRF2R.
NATURE COMMUNICATIONS
(2022)
Article
Neurosciences
Mariela Mitre, Khalil Saadipour, Kevin Williams, Latika Khatri, Robert C. Froemke, Moses V. Chao
Summary: The study shows that oxytocin can enhance the activity of BDNF in neurons by transactivating the TrkB receptor. This finding is important for understanding the mechanism of oxytocin and its relationship with neural development and social behaviors.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2022)
Review
Biology
Terry W. Moody, Irene Ramos-Alvarez, Robert T. Jensen
Summary: Cancer growth is regulated by receptor tyrosine kinases (RTKs) and G-protein-coupled receptors (GPCRs). Different mutations in EGFR and NTSR1 can lead to abnormal cancer proliferation. The use of tyrosine kinase inhibitors and SR48692 as antagonists can impair the transactivation process and inhibit cancer growth.
Article
Biochemistry & Molecular Biology
Richard Agren, Kristoffer Sahlholm
Summary: The study revealed that there is a difference in Ca2+ sensitivity between the two isoforms of dopamine D-2 receptor (D2LR and D2SR), with the desensitization rate of D2SR being influenced by co-expression of GRK2 and beta-arrestin2. The Ca2+ sensitive desensitization of D2SR appears to be mediated via a GRK2 phosphorylation-dependent mechanism.
Article
Pharmacology & Pharmacy
Gang Xie, Wenjie Liu, Zhen Lian, Dantao Xie, Guixin Yuan, Jiajie Ye, Zihong Lin, Weidong Wang, Jican Zeng, Huaxing Shen, Xinjia Wang, Haotian Feng, Wei Cong, Guanfeng Yao
Summary: The study demonstrated the inhibitory effects of the SYK inhibitor PRT on osteoclast and breast cancer functionalities in vitro and in vivo. PRT showed inhibition on osteoclastogenesis, bone resorption, and breast cancer cell growth, migration, and invasion, as well as prevention of bone loss post-OVX and cancer-induced bone destruction.
BIOCHEMICAL PHARMACOLOGY
(2021)
Review
Immunology
Nan Li, Shan Shan, Xiu-Qin Li, Ting-Ting Chen, Meng Qi, Sheng-Nan Zhang, Zi-Ying Wang, Ling-Ling Zhang, Wei Wei, Wu-Yi Sun
Summary: G protein-coupled receptor kinase 2 (GRK2) plays important roles in regulating signaling pathways associated with fibrotic diseases. Recent research suggests that GRK2 could be a potential therapeutic target for fibrotic diseases.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Oncology
Jin Ma, Jiang Ren, Midory Thorikay, Maarten van Dinther, Gonzalo Sanchez-Duffhues, Josselin Caradec, Pascal Benderitter, Jan Hoflack, Peter ten Dijke
Summary: Novel macrocyclic compounds OD16 and OD29 have been synthesized as inhibitors of BMP type I receptor and VEGF-induced signaling, demonstrating potential as anti-angiogenesis therapeutic agents by impairing endothelial cell function and inhibiting angiogenesis in both normal and tumour-induced conditions in vitro and in zebrafish embryos. These compounds show promise for overcoming therapy resistance and improving clinical efficacy in targeting angiogenesis pathways.
Article
Pharmacology & Pharmacy
Estefania Moreno, Nil Casajuana-Martin, Michael Coyle, Baruc Campos Campos, Ewa Galaj, Claudia Llinas del Torrent, Arta Seyedian, William Rea, Ning-Sheng Cai, Alessandro Bonifazi, Benjamin Floran, Zheng-Xiong Xi, Xavier Guitart, Vicent Casado, Amy H. Newman, Christopher Bishop, Leonardo Pardo, Sergi Ferre
Summary: This study provides evidence that heteromerization of G protein-coupled receptors (GPCRs), specifically dopamine D1 and D3 receptors, can influence the pharmacological properties of selective ligands. In vivo experiments support the involvement of D1R-D3R heteromers in the development of L-DOPA-induced dyskinesia in Parkinson's disease, suggesting the potential of targeting GPCR heteromers for drug development.
PHARMACOLOGICAL RESEARCH
(2022)
Article
Neurosciences
Wenqi Fu, Luca Franchini, Cesare Orlandi
Summary: This study generated new GPRC5B polyclonal antibodies and investigated the expression levels of GPRC5B in different organs and brain regions. It was found that GPRC5B had high levels of glycosylation in the mouse brain and was expressed at the highest level in the olfactory bulb, hippocampus, cerebellum, and pons. GABAergic neurons showed higher expression levels of Gprc5b than glutamatergic neurons in most regions.
FRONTIERS IN NEUROSCIENCE
(2022)
Article
Multidisciplinary Sciences
Maike Stegen, Andrea Engler, Crista Ochsenfarth, Iris Manthey, Jurgen Peters, Winfried Siffert, Ulrich H. Frey
Summary: The study characterized the human GRK6 promoter and found that the CREB binding site influenced promoter activity. Stimulation with a PKC activator led to decreased GRK6 expression at mRNA and protein levels, suggesting PKC might play a role in regulating GRK6 expression.
Article
Cardiac & Cardiovascular Systems
Si Chen, Yishuai Zhang, Janet K. Lighthouse, Deanne M. Mickelsen, Jiangbin Wu, Peng Yao, Eric M. Small, Chen Yan
Article
Immunology
Kensei Komatsu, Dae-Hwan Nam, Ji-Yun Lee, Go Yoneda, Chen Yan, Jian-Dong Li
JOURNAL OF IMMUNOLOGY
(2020)
Editorial Material
Medicine, Research & Experimental
Mary Wines-Samuelson, Sayantani Chowdhury, Bradford C. Berk
JOURNAL OF CLINICAL INVESTIGATION
(2020)
Review
Pharmacology & Pharmacy
Si Chen, Chen Yan
Summary: This review focuses on recent research advances on different PDE isoforms in the heart, discussing their expression patterns and biological functions. Current limitations and future directions in research are also addressed, along with the development of PDE inhibitors.
EXPERT OPINION ON DRUG DISCOVERY
(2021)
Article
Peripheral Vascular Disease
Chao Xue, Sharon Senchanthisai, Mark Sowden, Jinjiang Pang, Jim White, Bradford C. Berk
Article
Cardiac & Cardiovascular Systems
Hongmei Zhao, Hongqin Yang, Chi Geng, Yang Chen, Junling Pang, Ting Shu, Meijun Zhao, Yaqin Tang, Zhiwei Li, Baicun Li, Cuiliu Hou, Xiaomin Song, Aoxue Wu, Xiaoxiao Guo, Si Chen, Bin Liu, Chen Yan, Jing Wang
Summary: The study found that IgE plays a causative role in pathological cardiac remodeling, at least partially through activating IgE-Fc epsilon R1 signaling in cardiomyocytes and cardiac fibroblasts. Therapeutic strategies targeting the IgE-Fc epsilon R1 axis may be effective for managing IgE-mediated cardiac remodeling.
Article
Medicine, Research & Experimental
Jiangbin Wu, Kadiam C. Venkata Subbaiah, Feng Jiang, Omar Hedaya, Amy Mohan, Tingting Yang, Kevin Welle, Sina Ghaemmaghami, Wai Hong Wilson Tang, Eric Small, Chen Yan, Peng Yao
Summary: The study reveals that miR-574 plays a crucial role in cardiac diseases by regulating FAM210A expression and modulating mitochondrial-encoded protein expression, affecting cardiac remodeling.
EMBO MOLECULAR MEDICINE
(2021)
Article
Cardiac & Cardiovascular Systems
Lingfeng Luo, Yujun Cai, Yishuai Zhang, Chia G. Hsu, Vyacheslav A. Korshunov, Xiaochun Long, Peter A. Knight, Bradford C. Berk, Chen Yan
Summary: The study revealed that PDE10A contributes to SMC proliferation and intimal hyperplasia by antagonizing the CNP/NPR2/cGMP/PKG1alpha signaling pathway, suggesting that PDE10A may be a novel drug target for treating vascular occlusive disease.
CARDIOVASCULAR RESEARCH
(2022)
Correction
Cardiac & Cardiovascular Systems
Lingfeng Luo, Yujun Cai, Yishuai Zhang, Chia G. Hsu, Vyacheslav A. Korshunov, Xiaochun Long, Peter A. Knight, Bradford C. Berk, Chen Yan
CARDIOVASCULAR RESEARCH
(2023)
Review
Physiology
Michael P. Massett, Caitlyn Matejka, Hyoseon Kim
Summary: Inbred and genetically modified mice are often used for studying molecular mechanisms of beneficial adaptations to exercise training. However, the diversity in treadmill-based endurance exercise training protocols for mice makes comparisons difficult. Factors such as treadmill incline, training duration, exercise performance test, and outcome variables can significantly affect the efficacy of exercise training in mice, with training duration being the most significant factor.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Chia George Hsu, Camila Lage Chavez, Chongyang Zhang, Mark Sowden, Chen Yan, Bradford C. Berk
Summary: Pyroptosis is a form of cell death triggered by the innate immune system that is associated with the pathogenesis of sepsis and acute lung injury. A study found that the endogenous lipid 4-hydroxynonenal (HNE) can inhibit pyroptosis and inflammasome activation.
CELL DEATH AND DIFFERENTIATION
(2022)
Editorial Material
Cardiac & Cardiovascular Systems
Himanshu Meghwani, Bradford C. Berk
CIRCULATION RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Krishna R. Ojha, Hyoseon Kim, Samuel Padgham, Laura Hopkins, Robert J. Zamen, Abhijnan Chattopadhyay, Gang Han, Dianna M. Milewicz, Michael P. Massett, Andreea Trache
Summary: Thoracic aortic aneurysm is associated with ACTA2 pathogenic variants, and this study demonstrates that ACTA2(R149C/+) mutation leads to altered actin isoform expression and decreased integrin recruitment, resulting in reduced aortic contractility.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Physiology
Dylan Holly, Hyoseon Kim, Christopher R. Woodman, Michael P. Massett
Summary: This study assessed the influence of genetic background and sex on nitric oxide (NO)-mediated vasomotor function in arteries from different vascular territories. The results indicated that vasomotor responses vary by genetic background, sex, and artery type.
PHYSIOLOGICAL REPORTS
(2023)
Article
Physiology
Heather L. Vellers, Michael P. Massett, Josh J. Avila, Seung Kyum Kim, Jacqui M. Marzec, Janine H. Santos, J. Timothy Lightfoot, Steven R. Kleeberger
PHYSIOLOGICAL REPORTS
(2020)