期刊
ACS NANO
卷 9, 期 6, 页码 6128-6138出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsnano.5b01366
关键词
laser scanning lithography; image-guided patterning; biomimetic; cell-derived patterning; nanopatterning; self-assembled monolayers; actin cytoskeleton; cell adhesion; mechanotransduction; cell patterning; cell population heterogeneity; cell population homogeneity; cell engineering; cell arrays
类别
资金
- National Institutes of Health [P20EB007076, R01HL097520, R01EB005173, T32HL007676]
- National Science Foundation [NSF1150645]
- National Institutes of Health Nanobiology Training Program of the Keck Center of the Gulf Coast Consortia [T32DK070121]
- Howard Hughes Medical Institute
- Direct For Biological Sciences
- Div Of Biological Infrastructure [1262296] Funding Source: National Science Foundation
- Div Of Chem, Bioeng, Env, & Transp Sys
- Directorate For Engineering [1150645] Funding Source: National Science Foundation
Heterogeneity of cell populations can confound population-averaged measurements and obscure important findings or foster inaccurate conclusions. The ability to generate a homogeneous cell population, at least with respect to a chosen trait, could significantly aid basic biological research and development of high-throughput assays. Accordingly, we developed a high-resolution, image-based patterning strategy to produce arrays of single-cell patterns derived from the morphology or adhesion site arrangement of user-chosen cells of interest (COIs). Cells cultured on both cell-derived patterns displayed a cellular architecture defined by their morphology, adhesive state, cytoskeletal organization, and nuclear properties that quantitatively recapitulated the COls that defined the patterns. Furthermore, slight modifications to pattern design allowed for suppression of specific actin stress fibers and direct modulation of adhesion site dynamics. This approach to patterning provides a strategy to produce a more homogeneous cell population, decouple the influences of cytoskeletal structure, adhesion dynamics, and intracellular tension on nnechanotransduction-mediated processes, and a platform for high-throughput cellular assays.
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