期刊
CHROMATOGRAPHIA
卷 71, 期 11-12, 页码 1107-1111出版社
SPRINGER HEIDELBERG
DOI: 10.1365/s10337-010-1565-2
关键词
Column liquid chromatography; Tandem mass spectrometry; Gallic acid; Rhubarb; Pharmacokinetics
资金
- State Administration of Traditional Chinese Medicine [06-07ZP17]
- Chinese Natural Science Fund [30672587]
- Technology Innovation Program for post-graduate of Jiangsu province [CX08B_194Z]
Liquid chromatography-electrospray ionization tandem mass spectrometry has been used for rapid, selective, and sensitive quantitative analysis of gallic acid in rat plasma. Sample pretreatment involved a one-step extraction using ethyl acetate with protocatechic acid as internal standard. Separation was on a C(18) column using an isocratic mobile phase, consisting of methanol-0.1% aqueous formic acid (40:60, v/v) at 0.2 mL min(-1). The stability of gallic acid was evaluated in acidified and non-acidified plasma. The method was validated then successfully applied to a pharmacokinetic study in rats after oral administration of rhubarb extract.
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