Article
Biochemical Research Methods
Edvaldo Vasconcelos Soares Maciel, Fernando Mauro Lancas
Summary: This study presents a method that combines solid-packed cartridges and capillary LC-MS to integrate sample preparation and analytical steps. The proposed method effectively extracts and analyzes selected pesticides in soil samples, with potential applications in other solid samples. Initial qualitative tests on pesticides in honeybees were also conducted.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Zhiqi Zhang, Yaling Cai, Kai Fan, Qingwen Huang, Xiuying Zhao, Haojie Cao, Zhihui Zhao, Emmanuel K. Tangni, Zheng Han
Summary: A reliable UHPLC-MS/MS method was developed for simultaneous determination of two mycotoxins in various feed products. The method was thoroughly validated and successfully applied to investigate 60 feed samples, revealing a high contamination rate and wide concentration range of the mycotoxins. This developed method is a specific and reliable tool for intensive monitoring of mycotoxins in feed matrices.
FRONTIERS IN CHEMISTRY
(2022)
Article
Chemistry, Applied
Philipp Eyring, Marc Tienstra, Hans Mol, Susan Strange Herrmann, Peter Have Rasmussen, Henrik Lauritz Frandsen, Mette Erecius Poulsen
Summary: Various generic extraction methods have been used for determination of pesticide residues, mycotoxins, and PAHs in food and animal feed, but a new method introduced in this study facilitated identification of a wide range of compounds across different polarities. The method involved initial extraction with water, acetonitrile, formic acid, and heptane followed by solid-phase extraction clean-up and analysis by chromatography and mass spectrometry, achieving analyte recoveries with high accuracy and precision.
Article
Multidisciplinary Sciences
Nikola Ladislavova, Petra R. Pojmanova, Pavel R. Vrbka, Jana R. Snuparkova, Stepan R. Urban
Summary: This paper focuses on the chemical analysis of human scent samples from fired cartridge cases and their comparison with scent samples obtained under laboratory conditions. Comprehensive two-dimensional gas chromatography coupled with time-of-flight mass spectrometer was used for analyzing the scent samples. The results confirmed the stability of human scent evidence and its potential application in forensic purposes. The qualitative findings align with previous studies on the composition of human scent and the chemical composition of human fingerprints. Statistical analysis using similarity algorithms yielded a perfect identification rate of the scent inflictor when comparing the scent samples secured from fired cartridge cases with those collected under laboratory conditions.
Article
Chemistry, Analytical
Sophia A. Schreckenbach, Denina Simmons, Adam Ladak, Lauren Mullin, Derek C. G. Muir, Myrna J. Simpson, Karl J. Jobst
Summary: This study developed a suspect screening method based on industrial compounds with bioaccumulation potential. Results showed that SQDIA has a significantly lower false-positive rate compared to standard DIA. Through suspect screening and nontargeted screening, multiple pollutants were identified, with some compounds confirmed through standards and structural analysis.
ANALYTICAL CHEMISTRY
(2021)
Review
Medical Laboratory Technology
Kenichi Tamama
Summary: Drugs of abuse testing is commonly used in clinical and forensic settings, with urine being the preferred specimen type but other emerging types include saliva, sweat, hair, and meconium. Liquid chromatography-tandem mass spectrometry (LC-tandem-MS) has become the new gold standard for confirmatory drug testing due to its ability to analyze a wider range of analytes compared to gas chromatography-mass spectrometry (GC-MS). Technical advancements such as high-resolution mass spectrometry and miniature ambient ionization mass spectrometry have improved the speed and efficiency of drugs of abuse testing.
CLINICA CHIMICA ACTA
(2021)
Article
Multidisciplinary Sciences
Cara Olivier, Bianca Allen, Laneke Luies
Summary: This study optimized a urine preparation procedure for non-targeted metabolomics using GC-MS. The direct analysis method showed superior repeatability and higher metabolome coverage compared to the organic acid methods. The findings contribute to the advancement of urine metabolomics research.
SCIENTIFIC REPORTS
(2023)
Article
Food Science & Technology
Liyuan Wang, Zhengyan Hu, Jing Chen, Tianjiao Wang, Pinggu Wu, Ying Ying
Summary: This study developed a method to simultaneously detect a dozen preservatives in pastry using gas chromatography-mass spectrometry. The preservatives showed good linearity within the range of 1.0-50 μg/mL, with detection limits of 0.04-2.00 mg/kg and quantification limits of 0.12-6.67 mg/kg. The method is suitable for the determination of 12 preservatives in pastry.
Review
Chemistry, Analytical
A. Fernez Asensio, E. Alvarez-Gonzalez, A. Rodriguez, L. M. Sierra, E. Blanco-Gonzalez
Summary: Dysregulation of TCA cycle enzymes leads to abnormal accumulation of oncometabolites, which contribute to cancer formation and progression. GC-MS and LC-MS are powerful analytical tools for accurate identification and determination of these metabolites.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Applied
Petar Ristivojevic, Vesna Jovanovic, Dusanka Milojkovic Opsenica, Jihae Park, Judith M. Rollinger, Tanja Cirkovic Velickovic
Summary: A novel high-throughput methodological approach was developed for tracing compounds with radical scavenging and antimicrobial activities in brown seaweeds. Major components identified with these activities include stearidonic, eicosapentaenoic, and arachidonic acids. This method allows fast identification and quantification of bioactive compounds in multicomponent biological samples.
Article
Food Science & Technology
Mark M. Skinner, Jared T. Seale, Maranda S. Cantrell, Joseph M. Collins, Matthew W. Turner, Owen M. McDougal
Summary: This experimental study aimed to detect acrylamide in French fries using methods suitable for the food processing industry, comparing the pros and cons of each method to provide decision-making suggestions for food processors.
Article
Chemistry, Analytical
Ying Zhang, Bei Gao, Luis Valdiviez, Chao Zhu, Tara Gallagher, Katrine Whiteson, Oliver Fiehn
Summary: Stable isotope tracers are commonly used with gas chromatography coupled to mass spectrometry for enzyme activity and metabolic pathway studies. In this research, three commercially available instruments were used to analyze samples from a stable isotope labeling study on Rothia mucilaginosa metabolism. The study found that all three GC-MS instruments can be used for stable isotope tracing studies, with high-resolution GC-QTOF MS offering additional capabilities in identifying the chemical structures of unknown compounds.
ANALYTICAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Tsz Hei Fong, Wangpan Shi, Guohui Ruan, Siyi Li, Guanghui Liu, Leyun Yang, Kaibin Wu, Jingxian Fan, Chung Lam Ng, Yafang Hu, Haishan Jiang
Summary: This study retrospectively reviewed medical records and constructed a tuberculostearic acid (TBSA)-combined scoring system based on multiple factors, which showed promising results for predicting and diagnosing tuberculous meningitis (TBM).
Article
Food Science & Technology
Shi Nie, Laihao Li, Yueqi Wang, Yanyan Wu, Chunsheng Li, Shengjun Chen, Yongqiang Zhao, Di Wang, Huan Xiang, Ya Wei
Summary: This study identified and quantified volatile organic compounds (VOCs) in fermented sea bass using gas chromatography-ion mobility mass spectrometry (GC-IMS) and gas chromatography-mass spectrometry (GC-MS). Aldehydes were found to be the principal contributors to the overall flavor of fermented sea bass, and several characteristic VOCs were identified. GC-IMS was demonstrated to rapidly detect VOCs, providing a theoretical basis for studying flavor formation in fermented sea bass.
Article
Chemistry, Multidisciplinary
Kangyi Zhang, Lingling Gao, Can Zhang, Tao Feng, Haining Zhuang
Summary: A rapid and accurate method for detecting volatile components of corn was established using GC-IMS and GC-MS. The fingerprints of corns under different treatments were analyzed, and key flavor compounds were identified. The corns were classified into four categories, consistent with the results of sensory evaluation. Steamed, boiled, and fried corns were found to be more popular.
FRONTIERS IN CHEMISTRY
(2022)
Review
Agriculture, Multidisciplinary
Leina Dou, Yingjie Zhang, Yuchen Bai, Yuan Li, Minggang Liu, Shibei Shao, Qing Li, Wenbo Yu, Jianzhong Shen, Zhanhui Wang
Summary: This study discussed the application of chicken yolk antibody (IgY) in detecting chemical and biological hazards in food samples, presenting its advantages, structure, production methods, and principles of immunoassays. The future requires more types of IgY, increased sensitivity, and diversification in recognition-to-signal methods.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Infectious Diseases
Yehui Luan, Kexin Chen, Junjie Zhao, Linli Cheng
Summary: The study highlights the combined toxicity of four commonly used antibiotics on human hepatocytes. Synergistic toxicity was observed in certain combinations at specific concentrations, while others exhibited synergistic toxicity at almost all concentrations. The study emphasizes the significance of evaluating the mixing ratio in assessing the combined toxicity of antibiotics.
Article
Chemistry, Analytical
Yuchen Bai, Rui Liu, Leina Dou, Weilin Wu, Wenbo Yu, Kai Wen, Xuezhi Yu, Jianzhong Shen, Zhanhui Wang
Summary: This study investigated the influence of spacer arm length on antibody titer, affinity, and assay performance. It found that spacer arms of 6-8 carbon atoms induced the strongest antibody response, while shorter and longer spacer arms failed to do so.
ANALYTICA CHIMICA ACTA
(2023)
Review
Chemistry, Analytical
Liang Luo, Yantong Pan, Qing Li, Yingjie Zhang, Chaochao Chen, Jianzhong Shen, Zhanhui Wang
Summary: Adrenergic receptor agonists are analogues of adrenergic transmitters that bind and stimulate adrenergic receptors. They have been illegally used in animal husbandry for their growth-promoting and fat-reducing properties. It is important to detect these residues for food safety and human health.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Leina Dou, Qing Li, Yuchen Bai, Jiaqian Kou, Xiaonan Wang, Qian Zhao, Xuezhi Yu, Kai Wen, Zhanhui Wang, Jianzhong Shen, Wenbo Yu
Summary: Aggregation-induced emission luminogens (AIE-gens) have great potential for bacterial imaging and detection without complicated labeling or washing steps. In this study, we investigated the mechanism of their bacterial Light-Up behavior using large-scale molecular dynamics simulations. We proposed that the triphenylamine motif of TBP-1 plays a crucial role in the fluorescence turn-on and bacterial Light-Up. Based on this, we developed a heterogeneous lateral flow immunoassay (LFIA) for foodborne pathogen detection with improved sensitivity and specificity.
ANALYTICAL CHEMISTRY
(2023)
Article
Engineering, Environmental
Xiya Zhang, Yuchen Bai, Qianqian Tang, Minggang Liu, Li Nan, Kai Wen, Xuezhi Yu, Wenbo Yu, Jianzhong Shen, Zhanhui Wang
Summary: Antibody is a crucial biomolecule for the sensitivity and specificity of immunoassays. Predicting the effectiveness of hapten molecules before synthesis can improve success rate, cost-effectiveness, and controllability. In this study, a rational hapten design method was proposed and evaluated, leading to the production of highly specific antibodies with enhanced affinity, which were successfully applied in antigen detection.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Food Science & Technology
Changfei Duan, Huiyan Zhang, Yingjie Zhang, Qiang Li, Peipei Li, Ghulam Mujtaba Mari, Sergei A. Eremin, Jianzhong Shen, Zhanhui Wang
Summary: A highly sensitive, specific, robust, and rapid fluorescence polarization immunoassay (FPIA) was developed for the determination of erythromycin (ERY) in milk samples. The method utilized five tracers of ERY and three monoclonal antibodies to achieve high sensitivity. The developed FPIA showed a detection limit of 14.08 μg/L and recoveries of 96.08-107.77% with coefficients of variations of 3.41-10.97%. The total detection time was less than 5 minutes. The proposed FPIA method is a rapid, accurate, and simple screening tool for ERY in milk samples.
Editorial Material
Nanoscience & Nanotechnology
Yuan Li, Zhanhui Wang
Summary: This article describes some technical concerns for the single transgenic hybridoma selection method (STHSM) that we developed, and outlines how we innovated the specific procedures in the STHSM to address the questions raised by Listek's group.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Nanoscience & Nanotechnology
Xuesong Li, Jie Cheng, Kun Zeng, Shulin Wei, Jiaxuan Xiao, Yanyan Lu, Fang Zhu, Zhanhui Wang, Kun Wang, Xiangyang Wu, Zhen Zhang
Summary: Traditional hybridization chain reaction (HCR) is a popular isothermal amplification technique in bioanalysis, however, its slow kinetics limits its application. In this study, a poly DNA tetrahedrons (pTDNs)-mediated HCR was developed to enhance the kinetics by stretching the HCR initiator through electrostatic forces. The pTDNs-mediated HCR was successfully applied to develop a sensitive immunoassay for rapid detection of aflatoxin B1.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Analytical
Jie Fei, Jiaqun Jiang, Yuchen Bai, Weilin Wu, Xiangjun Zhao, Wenbo Yu, Kai Wen, Xuezhi Yu, Jianzhong Shen, Zhanhui Wang
Summary: This study demonstrates the importance of careful hapten design and extensive antibody screening in the development of sandwich immunoassays for small molecules, using ABM as an example. Through combinatorial associations of antibody pairs, a sandwich immunoassay for ABM was successfully established.
ANALYTICAL CHEMISTRY
(2023)
Article
Agriculture, Multidisciplinary
Jianyu Zhu, Jiaqian Kou, Licai Ma, Xuezhi Yu, Chenglong Li, Zhanhui Wang, Jianzhong Shen, Kai Wen, Wenbo Yu
Summary: Amatoxins are polypeptides that cause the majority of fatalities from unintentional consumption of poisonous mushrooms. This study focused on preparing high-affinity antibodies against amatoxins and investigating the recognition mechanism. The researchers also developed a fully automated immunoassay using a microfluidics chip, which showed improved sensitivity compared to conventional ELISA. This platform has important implications for confirming poison agents and diagnosing patients with mushroom poisoning.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Nanoscience & Nanotechnology
Xuesong Li, Jie Cheng, Kun Zeng, Shulin Wei, Jiaxuan Xiao, Yanyan Lu, Fang Zhu, Zhanhui Wang, Kun Wang, Xiangyang Wu, Zhen Zhang
Summary: A poly DNA tetrahedrons (pTDNs)-mediated hybridization chain reaction (HCR) was developed, which significantly enhanced the kinetics of HCR and was applied in the detection of aflatoxin B1. The increased G4 DNAzyme loaded on the second antibody contributed to the signal amplification. This work provides a promising approach to improve the speed and signal amplification of HCR in bioanalysis using simple DNA frameworks.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Agriculture, Multidisciplinary
Jianyu Zhu, Jiaqian Kou, Licai Ma, Xuezhi Yu, Chenglong Li, Zhanhui Wang, Jianzhong Shen, Kai Wen, Wenbo Yu
Summary: Researchers have developed a monoclonal antibody against amatoxins and investigated its recognition mechanism. They have also designed a microfluidics chip and a fully automated immunoassay for the rapid and accurate detection of amatoxins poisoning.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Chemistry, Analytical
Yuan Li, Peipei Li, Yuebin Ke, Xuezhi Yu, Wenbo Yu, Kai Wen, Jianzhong Shen, Zhanhui Wang
Summary: We developed an indirect competitive screening method for efficient selection of rabbit monoclonal antibodies (RmAbs) against small molecules. Using this method, we discovered a highly affinity RmAb against chloramphenicol (CAP) with unique halophilic properties. Molecular dynamic simulation revealed the stable structure of the RmAb and enhanced interactions with CAP in a saturated salt solution. Furthermore, the RmAb was utilized for the development of a sensitive immunoassay for CAP detection.
Article
Nanoscience & Nanotechnology
Yuan Li, Peipei Li, Yuebin Ke, Xuezhi Yu, Wenbo Yu, Kai Wen, Jianzhong Shen, Zhanhui Wang
Summary: This study developed a single transgenic hybridoma selection method (STHSM) using an artificial and stable on-cell-surface anchor, which improved the efficiency of monoclonal antibody discovery and resulted in antibodies with higher affinity compared to traditional methods.
ACS APPLIED MATERIALS & INTERFACES
(2022)
