期刊
CHINESE SCIENCE BULLETIN
卷 55, 期 22, 页码 2416-2421出版社
SCIENCE PRESS
DOI: 10.1007/s11434-010-3254-9
关键词
Rbp3; fatty acid composition; des genes; Synechocystis
资金
- National Natural Science Foundation of China [30825003]
- National Basic Research Program of China [2008CB418001]
- Chinese Academy of Sciences [KZCX1-YW-14-1]
In the cyanobacterium Synechocystis 6803, rbp3, a type-II RNA-binding protein gene, is slightly induced by temperature downshift. An rbp3 mutant shows significant reduction in total polyunsaturated fatty acids (PUFA) in membrane lipids. However, the reduction in PUFA has not attained the extent that would significantly affect the growth of the mutant at low temperature. Transcripts of fatty acid desaturase genes desA, desB and desD, and ccr-1, a gene required for growth at 15 degrees C, are significantly reduced in the mutant relative to the wild type, while transcripts of rbp1 (RNA-binding protein 1) and crhR (RNA helicase Light) are not affected. Rbp3 may directly or indirectly affect mRNA levels of certain genes.
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