Article
Chemistry, Multidisciplinary
Qiang Liu, Gunnar Knobloch, Jim Voorneveld, Nico J. Meeuwenoord, Herman S. Overkleeft, Gijsbert A. van Der Marel, Andreas G. Ladurner, Dmitri Filippov
Summary: The translation discusses the significance of ADP-ribosylation and the chemical synthesis of linear ADP-ribose oligomers, as well as their affinity for ALC1. The study reveals the length-dependent binding manner of ADPr oligomers to ALC1, illustrating how the activity of this molecular machine is controlled by PAR.
Article
Chemistry, Multidisciplinary
Hugo Minnee, Johannes G. M. Rack, Gijsbert A. van der Marel, Herman S. Overkleeft, Jeroen D. C. Codee, Ivan Ahel, Dmitri V. Filippov
Summary: ADP-ribosylation is a process that transfers an ADP ribose moiety to a nucleophilic side chain by consuming nicotinamide adenine dinucleotide, allowing for the regulation of key processes such as apoptosis and DNA repair. Recent studies have identified histidine as an acceptor site for ADP-ribose, leading to the development of a synthetic strategy for ADP-ribosylated histidine peptides. The stability and resistance of these peptides against chemical conditions and degradation by ADP-ribosylhydrolases have been investigated, showing promising results for their application in further research.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Multidisciplinary
Rui Hagino, Keita Mozaki, Naoko Komura, Akihiro Imamura, Hideharu Ishida, Hiromune Ando, Hide-Nori Tanaka
Summary: Poly(ADP-ribosylation) is a protein post-translational modification that generates branched poly(ADP-ribose). Through the development of a synthesis method, the branched core structure of poly(ADP-ribose) was successfully synthesized. This method overcame synthetic challenges, including the construction of glycosidic bonds in the presence of purine base and the installation of phosphate groups.
Article
Biochemistry & Molecular Biology
Yong Zhang, Qinqin Cheng, Xiao-Nan Zhang, Jiawei Li, Jingwen Chen, Yiling Wang
Summary: In this study, a PAR polymer-based bispecific antibody targeting both HER2 and T-cell CD3 antigens was developed. The conjugate had specific binding to target cells expressing HER2 and CD3, and exhibited potent cytotoxicity against HER2-positive breast cancer cells in the presence of non-activated PBMCs. Functionalized PAR polymers provide a new strategy for synthesizing bispecific antibodies with unique pharmacological activities for biomedical applications.
Article
Biology
Sridevi Challa, Keun W. Ryu, Amy L. Whitaker, Jonathan C. Abshier, Cristel Camacho, W. Lee Kraus
Summary: In this study, PAR Trackers were developed and characterized as highly sensitive tools for detecting ADPR in various experimental and biological systems, providing greater temporal and spatial precision.
Article
Chemistry, Organic
Jiaojiao Shen, Gongming Zhu, Yanbo You, Bianbian Huo, Fangyin Dai, Honglei Wang, Anlian Zhu, Lingjun Li
Summary: In this study, a poststage synthetic protocol for accessing novel ADP-2''-deoxyribosylderivatives through designing a light-induced biomimetic reaction was reported, and SPR assays revealed effective binding of ADP-2''-deoxyribosylpeptides to MacroH2A1.1 with a high affinity (K(D) = 3.75 x 10(-6) M).
Article
Biochemistry & Molecular Biology
David Hutin, Karoline Alvik Hagen, Peng Shao, Kim Sugamori, Denis M. Grant, Jason Matthews
Summary: PARP7 is an important regulator of the immune system, and is involved in intestinal inflammation. Loss of PARP7 impairs immune response to colon inflammation, possibly due to its role in IFN-I signaling rather than AHR signaling.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Samuele Lodovichi, Roberto Quadri, Sarah Sertic, Achille Pellicioli
Summary: In this study, it was discovered that inhibiting PARylation of the tumor suppressor gene BRCA1 leads to hyper-resection of DNA double-strand breaks (DSBs) through BRCA2 and the EXO1 nuclease. This process was accompanied by reduced barrier for resection and increased recruitment of the RAD51 recombinase. It was proposed that PARylation of BRCA1 is important in limiting the formation of excessively extended DNA filaments, thereby reducing illegitimate chromosome rearrangements. These findings provide insights into the mechanisms of gene recombination and PARP inhibitor treatment.
Article
Multidisciplinary Sciences
Johannes Gregor Matthias Rack, Qiang Liu, Valentina Zorzini, Jim Voorneveld, Antonio Ariza, Kourosh Honarmand Ebrahimi, Julia M. Reber, Sarah C. Krassnig, Dragana Ahel, Gijsbert A. van der Marel, Aswin Mangerich, James S. O. McCullagh, Dmitri V. Filippov, Ivan Ahel
Summary: Poly(ADP-ribosyl)ation is a versatile posttranslational modification involved in regulating cellular processes. This study focuses on the mechanisms of PAR removal by PARG and ARH3, highlighting their differences in hydrolysis of linear and terminal ADP-ribose linkages.
NATURE COMMUNICATIONS
(2021)
Editorial Material
Biochemistry & Molecular Biology
Michael S. Cohen
Summary: ADP-ribosylation is a crucial post-translational modification, involving MAR and PAR. Researchers have identified distinct MAR and PAR readers using a chemical biology approach.
Article
Chemistry, Multidisciplinary
Indranil Chatterjee, Deblina Roy, Gautam Panda
Summary: This article reports a scalable and environmentally friendly total synthesis method for a PARP inhibitor. Compared to existing synthesis methods, this method is operationally simple, highly atom economical, and environmentally friendly. The method also includes a key step of phosphonate-free synthesis, which is more convenient compared to previous synthesis methods.
Article
Biochemistry & Molecular Biology
Johannes Rudolph, Karolin Luger
Summary: The auxiliary factor HPF1 redirects the enzymatic activity of PARP1, altering the PAR modification of proteins and affecting DNA repair in cells.
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Maria Laura Kevorkian, Salome C. Vilchez Larrea, Silvia H. Fernandez Villamil
Summary: This study identifies the nuclear localization of TcPARP and its enrichment in the nucleolus of Trypanosoma cruzi. The study also reveals the presence of TcPARP in a thread-like structure connecting two dividing nuclei, and the detection of ADP-ribose polymers in this structure during mitosis. These findings shed light on new potential functions of TcPARP inside the nucleus and contribute to our understanding of its role in trypanosomatids beyond the well-described DNA damage response.
Article
Biology
Kelsie M. Rodriguez, Sara C. Buch-Larsen, Ilsa T. Kirby, Ivan Rodriguez Siordia, David Hutin, Marit Rasmussen, Denis M. Grant, Larry L. David, Jason Matthews, Michael L. Nielsen, Michael S. Cohen
Summary: PARP-7 has been identified as an important regulator of the innate immune response, with a key target being the inactive PARP family member PARP-13. Specifically, PARP-7 preferentially MARylates cysteine residues in the RNA binding zinc finger domain of PARP-13, as revealed by proteome-wide ADP-ribosylation analysis.
Article
Oncology
Sachiko Yamashita, Masakazu Tanaka, Chieri Ida, Kenichi Kouyama, Setsu Nakae, Taisuke Matsuki, Masataka Tsuda, Tsuyoshi Shirai, Kazuo Kamemura, Yoshisuke Nishi, Joel Moss, Masanao Miwa
Summary: This study investigates the regulation of cell proliferation by polyADP-ribosylation, revealing significantly higher levels of PAR in S phase compared to G1 phase, and a short half-life of PAR. Inhibition of PARP delays the cell cycle and decreases cell proliferation.
EXPERIMENTAL CELL RESEARCH
(2022)
Article
Biochemical Research Methods
Vera Bilan, Nathalie Selevsek, Hans A. V. Kistemaker, Jeannette Abplanalp, Roxane Feurer, Dmitri V. Filippov, Michael O. Hottiger
MOLECULAR & CELLULAR PROTEOMICS
(2017)
Article
Chemistry, Multidisciplinary
Lucien N. Lameijer, Tobias G. Breve, Vincent H. S. van Rixel, Sven H. C. Askes, M. A. Siegler, Sylvestre Bonnet
CHEMISTRY-A EUROPEAN JOURNAL
(2018)
Article
Chemistry, Organic
Lucien N. Lameijer, Julien le Roy, Stefan van der Vorm, Sylvestre Bonnet
JOURNAL OF ORGANIC CHEMISTRY
(2018)
Article
Chemistry, Multidisciplinary
Lucien N. Lameijer, Corjan van de Griend, Samantha L. Hopkins, Anne-Geert Volbeda, Sven H. C. Askes, Maxime A. Siegler, Sylvestre Bonnet
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2019)
Article
Chemistry, Multidisciplinary
Vincent H. S. van Rixel, Vadde Ramu, Austin B. Auyeung, Nataliia Beztsinna, David Y. Leger, Lucien N. Lameijer, Stan T. Hilt, Sylvia E. Le Devedec, Tugba Yildiz, Tania Betancourt, M. Brenton Gildner, Todd W. Hudnall, Vincent Sol, Bertrand Liagre, Alexander Kornienko, Sylvestre Bonnet
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2019)
Article
Chemistry, Multidisciplinary
Lucien N. Lameijer, Simon Budzak, Nadja A. Simeth, Mickel J. Hansen, Ben L. Feringa, Denis Jacquemin, Wiktor Szymanski
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2020)
Article
Chemistry, Multidisciplinary
Jim Voorneveld, Max S. Kloet, Sven Wijngaarden, Robbert Q. . Kim, Angeliki Moutsiopoulou, Marnix Verdegaal, Mohit Misra, Ivan Dikic, Gijsbert A. . van der Marel, Herman S. Overkleeft, Dmitri V. Filippov, Gerbrand J. van der Heden van Noort
Summary: This article describes the development and optimization of a methodology for preparing peptides and proteins modified on the arginine residue with an ADPr group. The authors successfully obtained four regioisomers of ADP-ribosylated ubiquitin using this method and investigated the activity and selectivity of Legionella pneumophila ligase and hydrolase enzymes through biochemical experiments.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Chemistry, Multidisciplinary
Miroslav Medved, Mariangela Di Donato, Wybren Jan Buma, Adele D. Laurent, Lucien Lameijer, Tomas Hrivnak, Ivan Romanov, Susannah Tran, Ben L. Feringa, Wiktor Szymanski, G. Andrew Woolley
Summary: Azonium ions derived from aminoazobenzenes with ortho-methoxy substitutions isomerize under red light, which makes them useful for the photocontrol of physiological processes. This study provides a mechanistic understanding of the isomerization process and thermal relaxation, and suggests improvements for the design of derivatives. The study also reveals the impact of solution pH on the photoswitching behavior of azonium ions.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Chemistry, Multidisciplinary
Miroslav Medved, Mariangela Di Donato, Wybren Jan Buma, Adele D. Laurent, Lucien Lameijer, Tomas Hrivnak, Ivan Romanov, Susannah Tran, Ben L. Feringa, Wiktor Szymanski, G. Andrew Woolley
Summary: Azonium ions formed by the protonation of aminoazobenzenes can undergo photoisomerization with red light under physiological conditions. By combining transient absorption measurements and quantum chemical calculations, the researchers revealed the mechanistic details of the photon-induced isomerization process. This understanding can guide the design of improved azonium ion derivatives for better photocontrol applications.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Chemistry, Multidisciplinary
Kaja Sitkowska, Martijn F. Hoes, Michael M. Lerch, Lucien N. Lameijer, Peter van der Meer, Wiktor Szymanski, Ben L. Feringa
CHEMICAL COMMUNICATIONS
(2020)
Article
Chemistry, Multidisciplinary
Lucien N. Lameijer, Daniel Ernst, Samantha L. Hopkins, Michael S. Meijer, Sven H. C. Askes, Sylvia E. Le Devedec, Sylvestre Bonnet
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2017)
Article
Biochemistry & Molecular Biology
Fredj Ben Bdira, Wouter W. Kallemeijn, Saskia V. Oussoren, Saskia Scheij, Boris Bleijlevens, Bogdan I. Florea, Cindy P. A. A. van Roomen, Roelof Ottenhoff, Marielle J. F. M. van Kooten, Marthe T. C. Walvoort, Martin D. Witte, Rolf G. Boot, Marcellus Ubbink, Herman S. Overkleeft, Johannes M. F. G. Aerts
ACS CHEMICAL BIOLOGY
(2017)
Article
Biochemistry & Molecular Biology
Emily S. Weyburne, Owen M. Wilkins, Zhe Sha, David A. Williams, Alexandre A. Pletnev, Gerjan de Bruin, Hermann S. Overkleeft, Alfred L. Goldberg, Michael D. Cole, Alexei F. Kisselev
CELL CHEMICAL BIOLOGY
(2017)
Review
Chemistry, Multidisciplinary
Daan van der Es, Wouter F. J. Hogendorf, Herman S. Overkleeft, Gijsbert A. van der Marel, Jeroen D. C. Codee
CHEMICAL SOCIETY REVIEWS
(2017)
Article
Chemistry, Multidisciplinary
Sander B. Engelsma, Nico J. Meeuwenoord, Hermen S. Overkleeft, Gijsbert A. van der Marel, Dmitri V. Filippov
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2017)