Article
Immunology
Maxim Shkurnikov, Stepan Nersisyan, Tatjana Jankevic, Alexei Galatenko, Ivan Gordeev, Valery Vechorko, Alexander Tonevitsky
Summary: The study found an association between HLA class I genotypes and the age of deceased patients with COVID-19, and developed a risk score model based on different HLA alleles to predict disease severity. The results suggest the important role of HLA class I peptide presentation in the development of a specific immune response to COVID-19.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Kenji Sugata, Yukiko Matsunaga, Yuki Yamashita, Munehide Nakatsugawa, Tingxi Guo, Levon Halabelian, Yota Ohashi, Kayoko Saso, Muhammed A. Rahman, Mark Anczurowski, Chung-Hsi Wang, Kenji Murata, Hiroshi Saijo, Yuki Kagoya, Dalam Ly, Brian D. Burt, Marcus O. Butler, Tak W. Mak, Naoto Hirano
Summary: By combining molecular biological and immunological techniques, this study successfully cloned sequences encoding HLA-DP, HLA-DQ, and HLA-DR molecules with enhanced CD4 binding affinity and produced affinity-matured class II dimers that stain antigen-specific T cells better than conventional multimers. These affinity-matured class II dimers will aid in the investigation of human CD4(+) T-cell responses, providing a comprehensive library of dimers for HLA-DP, HLA-DQ, and HLA-DR alleles. The readily detectable CD4(+) T cells with class II MHC dimers are crucial for studying human immune responses.
NATURE BIOTECHNOLOGY
(2021)
Review
Immunology
Laura Santambrogio
Summary: Advances in the analysis of the MHC class II ligandome have increased our understanding of the factors that regulate the range and selection of presented peptides, and the landscape is highly sensitive to changes in protein composition.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
M. Cortazar-Chinarro, A. Richter-Boix, P. Rodin-Morch, P. Halvarsson, J. B. Logue, A. Laurila, J. Hoglund
Summary: Microbiomes play a crucial role in determining the ecology and behavior of their hosts. This study investigated the effects of geography, evolutionary history, and host genetics on the skin microbiome diversity and structure in a widespread amphibian. The results showed that microbiome diversity was correlated with host genetic diversity, and the bacterial community composition varied between geographical clusters and specific host genetic variations.
Article
Immunology
Yanan Wu, Nianzhi Zhang, Keiichiro Hashimoto, Chun Xia, Johannes M. Dijkstra
Summary: The structures of peptide-loaded major histocompatibility complex class I (pMHC-I) and class II (pMHC-II) complexes are similar, but there are differences in the components. Comparative analysis of primitive vertebrate species' pMHC-I and pMHC-II structures sheds light on the evolution of MHC structures. The establishment of pMHC-I involved the development of important new functions that have been well conserved since their early inception.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Cell Biology
Jihong He, Jun Chen, Xue Han, Qin Gu, Jiangli Liang, Mingbo Sun, Shuyuan Liu, Yufeng Yao, Li Shi
Summary: This study found that variations in the HLA-DM and HLA class II genes may influence the expression of neutralising antibodies (NAbs) induced by the inactivated Japanese encephalitis vaccine (IJEV) at both the seroconversion and geometric mean titres (GMT) levels. DQB1*02:01 was positively associated with NAbs, while DQB1*02:02 was negatively associated. Additionally, different haplotypes were associated with the expression levels of NAbs.
Article
Immunology
Mark N. Lee, Matthew Meyerson
Summary: This study introduces a high-throughput epitope identification system that combines T cell-secreted cytokines capture, cell sorting, and next-generation sequencing to identify new class I- and class II-restricted epitopes. The technology successfully validated known viral, neoepitope, and autoimmune epitope-specific TCR targets, as well as discovered new epitopes encoded by the human cytomegalovirus genome. This cytokine capture-based assay enables pooled screening of thousands of encoded peptides for epitope discovery and may lead to identification of HLA-epitope-TCR complexes relevant to disease control, etiology, or treatment.
SCIENCE IMMUNOLOGY
(2021)
Article
Biochemical Research Methods
Niclas Olsson, Wei Jiang, Lital N. Adler, Elizabeth D. Mellins, Joshua E. Elias
Summary: This study investigates the effects of DO:DM ratios on peptide presentation by creating a panel of HLA-DR4-expressing APC lines and analyzing over 10,000 unique DR4-presented peptides. The findings provide insights into the characteristics that influence peptide presentation and have implications for HLA-II prediction algorithms and research strategies.
MOLECULAR & CELLULAR PROTEOMICS
(2022)
Article
Genetics & Heredity
Esteban Arrieta-Bolanos, Diana Iraiz Hernandez-Zaragoza, Rodrigo Barquera
Summary: HLA frequencies show variation across human populations. Geography has a strong effect on the distribution of HLA class I allele groups, while class II frequencies correlate less clearly to actual geographic location. The study also reveals intra-continental sub-clusters and characteristic HLA allele group patterns.
FRONTIERS IN GENETICS
(2023)
Article
Oncology
Ila J. Datar, Sacha C. Hauc, Shruti Desai, Nicole Gianino, Brian Henick, Yuting Liu, Kostas Syrigos, David L. Rimm, Paula Kavathas, Soldano Ferrone, Kurt A. Schalper
Summary: This study analyzed the distribution, immune contexture, and clinical significance of HLA class-I and -II subunits in NSCLC. The results revealed frequent and differential defects in HLA protein subunit expression in NSCLCs, associated with distinct tumor microenvironment composition and patient survival.
CLINICAL CANCER RESEARCH
(2021)
Article
Immunology
Yue Shen, Jerry M. Parks, Jeremy C. Smith
Summary: This study presents a method for classifying HLA class I molecules using three-dimensional structures, which improves the breadth, accuracy, stability, and flexibility of classification. The findings show that structural similarity is highly correlated with peptide binding specificity. This new classification is useful in peptide-based vaccine development and HLA-disease association studies.
JOURNAL OF IMMUNOLOGY
(2023)
Article
Immunology
Rodrigo Ochoa, Victoria Alves Santos Lunardelli, Daniela Santoro Rosa, Alessandro Laio, Pilar Cossio
Summary: In this study, researchers developed a method called PanMHC-PARCE for engineering multiple-allele binders by optimizing epitope sequence through mutations and simulations. They successfully improved the binding affinity of a Plasmodium vivax epitope for multiple human MHC II alleles. Additionally, in vivo experiments showed that immunization with the engineered peptides induced interferon-gamma cellular immune response in mice. This method contributes to the design of new immunotherapies.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Genetics & Heredity
Jana Sticht, Miguel Alvaro-Benito, Stefan Konigorski
Summary: Type 1 diabetes is an autoimmune disease with increasing incidence in high-income countries. Genetic and environmental factors play important roles in its pathogenesis. A comprehensive genetic association analysis of the HLA region can provide deeper insights into the etiology of the disease.
FRONTIERS IN GENETICS
(2021)
Article
Immunology
Yuri Poluektov, Marybeth George, Pirouz Daftarian, Marc C. Delcommenne
Summary: The study focuses on evaluating the binding affinities of SARS-CoV-2 peptides to different MHC alleles using the QuickSwitchTM platform, identifying multiple MHC binders with high promiscuity. These results provide important data for further research on the SARS-CoV-2 virus and its antigenic epitopes.
Article
Oncology
Simone Stupia, Christina Heeke, Alicia Bruggemann, Anne Zaremba, Beatrice Thier, Julia Kretz, Antje Sucker, Manuel Philip, Gennadiy Zelinskyy, Soldano Ferrone, Alexander Roesch, Susanne Horn, Eva Hadaschik, Dirk Schadendorf, Mirko Trilling, Ulf Dittmer, Klaus Griewank, Fang Zhao, Annette Paschen
Summary: Recent studies have shown that cytotoxic CD4 T cells can kill melanoma cells dependent on HLA class II (HLA-II). This study investigates the evolution of HLA-II-loss tumors that evade cytotoxic CD4 T cell activity and contribute to immunotherapy resistance. The findings highlight the importance of tumor cell-intrinsic HLA-II antigen presentation in disease control and suggest the need to overcome its down-regulation for better patient outcomes.
CLINICAL CANCER RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Sundeep Malik, Terrance M. Dolan, Zachary J. Maben, Patricia M. Hinkle
JOURNAL OF BIOLOGICAL CHEMISTRY
(2015)
Article
Endocrinology & Metabolism
Zachary J. Maben, Sundeep Malik, Liyi H. Jiang, Patricia M. Hinkle
FRONTIERS IN ENDOCRINOLOGY
(2016)
Article
Biochemistry & Molecular Biology
Anastasia Mpakali, Zachary Maben, Lawrence J. Stern, Efstratios Stratikos
MOLECULAR IMMUNOLOGY
(2019)
Article
Immunology
Michael D. Valentino, Zachary J. Maben, Lucinda L. Hensley, Matthew D. Woolard, Thomas H. Kawula, Jeffrey A. Frelinger, John G. Frelinger
Article
Biochemical Research Methods
Michael D. Valentino, C. Siddiq Abdul-Alim, Zachary J. Maben, Denise Skrombolas, Lucinda L. Hensley, Thomas H. Kawula, Michelle Dziejman, Edith M. Lord, Jeffrey A. Frelinger, John G. Frelinger
JOURNAL OF IMMUNOLOGICAL METHODS
(2011)
Article
Biochemistry & Molecular Biology
Athanasios Stamogiannos, Zachary Maben, Athanasios Papakyriakou, Anastasia Mpakali, Paraskevi Kokkala, Dimitris Georgiadis, Lawrence J. Stern, Efstratios Stratikos