Effects of Modified Sanhuang Decoction (aS a3aparts per thousande⟫ae±currency sign) enema on serum tumor necrosis factor-α and colonic mucosa interleukin-1β, interleukin-6 levels in ulcerative colitis rats

To investigate the effects of Modified Sanhuang Decoction (aS a(3)a parts per thousand e >>ae +/- currency sign, MSD) enema on the serum tumor necrosis factor alpha (TNF-alpha) and colonic mucosa interleukin-1 beta (IL-1 beta), interleukin-6 (IL-6) levels in experimental ulcerative colitis (UC) rats. Forty-five male Wistar rats were randomly divided into 4 groups: normal group (n=12), model group (n=11), salazosulfapyridine (SASP) group (n=11) and MSD group (n=11). The UC model was induced by 2,4,6-trinitrobenzene sulfonic acid (TNBS)/ethanol solution. Rats in the normal group and model group were clystered with 0.9% normal saline, while in the SASP group and MSD group were clystered with SASP and MSD enema, respectively. After drug administration (10 mL/kg body weight, for 7 days), colonic gross changes and colonic mucosa histology were observed, serum TNF-alpha and colonic mucosa IL-1 beta, IL-6 levels were tested by enzyme linked immunosorbent assay and radioimmunoassay, respectively. As compared with the normal group, the experimental UC rats, the colonic mucosal damage index scores (CMDIs), histopathological scores (HS) and the serum TNF-alpha and colonic mucosa IL-1 beta, IL-6 levels significantly increased (P < 0.05 or P < 0.01). In the MSD and SASP groups, the ulcer area significantly reduced, and edema disappeared. The CMDIs, HS, the serum TNF-alpha and colonic mucosa IL-1 beta, IL-6 levels in the MSD and SASP groups significantly decreased (P < 0.05 or P < 0.01) compared with the model group. The CMDIs in the MSD group were lower than that in the SASP group (P < 0.05), but there were no significant differences in HS, serum TNF-alpha or colonic mucosa IL-1 beta, IL-6 levels between the MSD and SASP groups. MSD enema can improve colonic mucosa impairment and decrease serum TNF-alpha and colonic mucosa IL-1 beta, IL-6 levels in experimental UC.