期刊
CHINESE JOURNAL OF CHEMISTRY
卷 30, 期 5, 页码 1163-1167出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cjoc.201100452
关键词
graphene; glucose oxidase; direct electrochemistry; biosensor; electroanalysis
资金
- National Natural Science Foundation of China [21161003, 20701010]
- Guangxi Natural Science Foundation of China [0991093, 2011 GXNSFA018044, 2010GXNSFF013001]
- Guangxi Education Department of China [201012MS024]
A simple and effective glucose biosensor based on immobilization of glucose oxidase (GOD) in graphene (GR)/Nafion film was constructed. The results indicated that the immobilized GOD can maintain its native structure and bioactivity, and the GR/Nafion film provides a favorable microenvironment for GOD immobilization and promotes the direct electron transfer between the electrode substrate and the redox center of GOD. The electrode reaction of the immobilized GOD shows a reversible and surface-controlled process with the large electron transfer rate constant (ks) of 3.42 +/- 0.08 s-1. Based on the oxygen consumption during the oxidation process of glucose catalyzed by the immobilized GOD, the as-prepared GOD/GR/Nafion/GCE electrode exhibits a linear range from 0.5 to 14 mmol.L-1 with a detection limit of 0.03 mmol.L-1. Moreover, it displays a good reproducibility and long-term stability.
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