Article
Biotechnology & Applied Microbiology
Tinuola Olorunsogbon, Yinka Adesanya, Hasan K. Atiyeh, Christopher Chukwudi Okonkwo, Victor Chinomso Ujor, Thaddeus Chukwuemeka Ezeji
Summary: This study provides evidence on the effectiveness of metabolically engineered C. beijerinckii NCIMB 8052 for fermenting LB-derived hydrolysates to produce acetone-butanol-ethanol (ABE). The engineered strain showed higher butanol and ABE production in non-detoxified hydrolysates compared to the wildtype strain, and even higher production in detoxified hydrolysates.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Marcelo Rivas-Astroza, Ivan Paredes, Karlo Guerrero, Silvia Mau, Julian Quintero, Juan Carlos Gentina, Raul Conejeros, German Aroca
Summary: A new kinetic model of Clostridium beijerinckii's Acetone-Butanol-Ethanol fermentation, considering three cell types, accurately replicates batch culture data. The model estimates cell type-specific kinetic parameters, which can improve the operation of ABE fermentation and provide a framework for studying acidogenic and solventogenic metabolic pathways.
JOURNAL OF BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Bunmi. B. B. Omorotionmwan, Hengzheng Wang, Jonathan. P. P. Baker, Krzysztof Gizynski, Minyeong Yoo, Cynthia Akaluka, Ying Zhang, Nigel. P. P. Minton
Summary: Engineered microbial chassis using fermentation processes have the potential to replace environmentally damaging petrochemical feedstocks by recycling biomass-derived carbon into chemicals and fuels. Multiple marked auxotrophic strains of Clostridium acetobutylicum were created to allow rapid integration of heterologous genes at distinct loci using allele-coupled exchange. The system was demonstrated to successfully produce isopropanol by integrating synthetic acetone operon and a gene encoding secondary dehydrogenase into specific loci.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Multidisciplinary Sciences
Lihua Lin, Zhikai Zhang, Hongchi Tang, Yuan Guo, Bingqing Zhou, Yi Liu, Ribo Huang, Liqin Du, Hao Pang
Summary: Introduction of a heterologous pathway for sucrose transport and metabolism in Clostridium beijerinckii significantly enhanced n-butanol production. The expression of sucrose transporter and invertase genes from other organisms improved acetone-butanol-ethanol production using sucrose-based substrates.
ROYAL SOCIETY OPEN SCIENCE
(2021)
Article
Agricultural Engineering
Carla Ferreira dos Santos Vieira, Mateus Cavichioli Codogno, Francisco Maugeri Filho, Rubens Maciel Filho, Adriano Pinto Mariano
Summary: This study demonstrates that Clostridium beijerinckii DSM 6423 is able to co-ferment first- and second-generation sugars while consuming lactic acid, resulting in high butanol yield.
BIORESOURCE TECHNOLOGY
(2021)
Article
Agricultural Engineering
Yinka Adesanya, Hasan K. Atiyeh, Tinuola Olorunsogbon, Asmita Khanal, Christopher C. Okonkwo, Victor C. Ujor, Ajay Shah, Thaddeus C. Ezeji
Summary: The study investigated a process engineering strategy to effectively convert hydrothermolysis pretreated non-detoxified switchgrass hydrolysates to acetone butanol ethanol (ABE) using a metabolically engineered strain of Clostridium beijerinckii. Through modifications to the engineered strain and the use of intermittent feeding, ABE fermentation was enhanced and detoxification of the hydrolysates prior to fermentation was eliminated. The ABE production from the non-detoxified hydrolysates by the engineered strain was comparable to the control medium, demonstrating the potential of this strategy in improving biofuel production.
BIORESOURCE TECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Juan Carlos Quintero-Diaz, Diego F. Mendoza, Claudio Avignone-Rossa
Summary: In this study, a kinetic model of acetone-butanol-ethanol (ABE) fermentation based on enzyme kinetics expressions was proposed. The model takes into account the effect of the co-substrate NADH as a modulating factor of cellular metabolism. The simulations obtained with the model showed a good fit to experimental data, surpassing previous models. The introduction of NADH in the model offers a basis for analyzing electrofermentation processes with Clostridium.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Engineering, Environmental
Marwa M. El-Dalatony, Bikram Basak, Mayur B. Kurade, Hyun-Seog Roh, Min Jang, Byong-Hun Jeon
Summary: This study demonstrates the effective production of biohydrogen (bioH(2)) and ABE through ABE fermentation using sonication pretreated Chlamydomonas mexicana biomass as a cost-effective substrate. Sonication enhances the recovery and bioaccessibility of carbohydrates and proteins, resulting in higher yields of bioH(2) and volatile fatty acids. The study shows the efficient conversion of soluble carbohydrates and proteins into bioH(2) and ABE, which can be used for high-energy biofuel production with minimal waste generation.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2022)
Article
Energy & Fuels
Zezhou Guo, Xiumin Yu, Yaodong Du, Tianqi Wang
Summary: With the increasing regulatory requirements for vehicles powered by internal combustion engines, improving engine thermal efficiency and reducing harmful emissions has become urgent. Alcohols, known for their renewability and cleaner emissions, have attracted wide attention. Among them, bio-butanol, which is most similar to gasoline, has been widely studied in spark ignition (SI) engines. However, the high energy consumption during the separation and purification process in the fermentation of bio-butanol results in its high price. ABE and IBE, as intermediates for the preparation of butanol through different fermentation methods, can be directly used in SI engines, significantly reducing the cost. Combined injection is the most suitable injection approach for dual-fuel engines, as it allows flexible control of the dual-fuel injection ratio and parameters. Therefore, this study experimentally compared the combustion and emissions of gasoline/ABE, gasoline/IBE, and gasoline/butanol using gasoline port injection plus alcohol direct injection mode. The results show that gasoline/IBE performs better in terms of power performance and brake thermal efficiency (BTE) compared to gasoline/ABE and gasoline/butanol. Moreover, when the direct injection ratio (DIr) is low, the gaseous emissions of gasoline/ABE are superior, while emissions of gasoline/butanol are superior at high DIr. In addition, the particle number (PN) of gasoline/ABE is the lowest among the three modes, and the particles exhibit a unimodal distribution. Overall, as direct injection fuel in dual-fuel combined injection engines, ABE and IBE have more advantages than butanol in terms of combustion and emissions performance as well as cost.
Article
Green & Sustainable Science & Technology
Mutlu Okcu, Yasin Varol, Sehmus Altun, Mujdat Firat
Summary: The study investigated the impact of using low reactivity fuel IBE in an RCCI engine and found the optimal premixed ratio for combustion characteristics and NOx emissions under different loads.
SUSTAINABLE ENERGY TECHNOLOGIES AND ASSESSMENTS
(2021)
Article
Energy & Fuels
Sarita Sae-hun, Piyawat Chinwatpaiboon, Akarin Boonsombuti, Ancharida Savarajara, Apanee Luengnaruemitchai
Summary: This study applied immobilization technique to improve cell culture in ABE fermentation and selected cassava rhizome charcoal, bamboo charcoal, and coconut shell activated carbon as carriers. The results showed that the immobilized cells on bamboo charcoal produced higher butanol than free cells, and cassava rhizome charcoal exhibited the highest cell efficiency after sequential reuse cycles.
BIOMASS CONVERSION AND BIOREFINERY
(2022)
Article
Biochemistry & Molecular Biology
Carlos Alberto Garcia Mogollon, Juan Carlos Quintero Diaz, Jorge Omar Gil Posada
Summary: This manuscript examines the impact of modifying the extracellular redox potential on the production of acetone, butanol, and ethanol in a microbial fuel cell. The addition of NADH enhances acetone production, while adjusting the cathode potential favors butanol production. The findings highlight the potential of bio-electrochemical systems to improve conventional fermentation processes.
BIOELECTROCHEMISTRY
(2023)
Article
Biochemical Research Methods
Sireethorn Khaonuan, Rattana Jariyaboon, Nikannapas Usmanbaha, Benjamas Cheirsilp, Nils-Kare Birkeland, Prawit Kongjan
Summary: Marine macroalgae, specifically Gracilaria tenuistipitata, showed the highest potential for butanol production through acetone-butanol-ethanol fermentation. Low-temperature hydrothermal pretreatment of G. tenuistipitata resulted in a high reducing sugar yield rate and ABE yield.
BIOTECHNOLOGY JOURNAL
(2023)
Article
Agricultural Engineering
Juan C. Lopez-Linares, Maria Teresa Garcia-Cubero, Monica Coca, Susana Lucas
Summary: The study proposes a method to valorize potential sugars from spent coffee grounds and produce biobutanol under optimal conditions, achieving high enzymatic hydrolysis yields and butanol production.
BIORESOURCE TECHNOLOGY
(2021)
Article
Agricultural Engineering
Carla Ferreira dos Santos Vieira, Augusto Duzi Sia, Francisco Maugeri Filho, Rubens Maciel Filho, Adriano Pinto Mariano
Summary: The productivity of Isopropanol-Butanol-Ethanol can be increased by immobilizing cells on low-cost, renewable fibrous materials, with in-situ vacuum product recovery technique applied for repeated-batch cultivation. The fermentation process showed improved IBE productivity compared to free- and immobilized-cell cultures, with the product stream containing 45 g/L IBE. However, unsatisfactory results were obtained in two cycles, leading to the introduction of a concept of an internal-loop boiling-driven fibrous-bed bioreactor.
BIORESOURCE TECHNOLOGY
(2022)
Article
Polymer Science
Figen V. Yaylali, Hande Ozel, Yasemin A. Udum, Levent Toppare, Saniye Soylemez, Gorkem Gunbas
Summary: Research on synthesizing sulfur-containing derivatives of bridged dioxythiophene based polymers (PEDOT and PProDOT) for electrochromic applications has shown promising results. A novel electron rich monomer, 3,4-dihydro-2H-thieno [3,4-b] [1,4]oxathiepine (ProTOT), has been successfully synthesized and used as the donor unit in a D-A-D monomer, resulting in the creation of P(ProTThia) with excellent electrochromic properties. Additionally, a glucose biosensor utilizing P(ProTThia), chitosan, and multi-walled carbon nanotubes has been proposed as an effective and inexpensive alternative for highly sensitive and fast response glucose detection.
Article
Chemistry, Medicinal
Sultan Cetin, Zubeyir Elmazoglu, Osman Karaman, Hande Gunduz, Gorkem Gunbas, Safacan Kolemen
Summary: Iodination of the silicon-fluorescein core resulted in a new class of cytotoxic, red-shifted, water-soluble photosensitizer SF-I, with good emissive properties and singlet oxygen generation capacity. SF-I showed efficient singlet oxygen generation and photocytotoxicity in triple negative breast and colon cancer cell lines, indicating great potential as a small-molecule theranostic agent.
ACS MEDICINAL CHEMISTRY LETTERS
(2021)
Article
Engineering, Chemical
Scott Svadlenak, Skip Rochefort, Konstantinos A. Goulas
Summary: The gasification behavior of polymer waste is correlated to the energy of the weakest C-H bond in the polymer structure. The use of adsorbents and reforming catalysts can enhance syngas yield while promoting gasification and reforming over pyrolysis reactions. The Ni/Al2O3 catalyst shows stability over multiple recycle reactor runs and demonstrates synergistic effects during the gasification of polymer blends.
Article
Chemistry, Physical
Tanmayi Bathena, Truc Phung, Scott R. Svadlenak, Yu Liu, Lars C. Grabow, Konstantinos A. Goulas
Summary: In this study, PdxCuy/SiO2 and PdxAgy/SiO2 catalysts were investigated for oxygenate upgrading in biofuels. The selectivity was found to be mainly influenced by geometric effects, while the reaction rate of the catalysts was dominated by the electronic effects of alloying.
Article
Chemistry, Physical
Kubra Nur Ozvural Sertcelik, Osman Karaman, Toghrul Almammadov, Gorkem Gunbas, Safacan Kolemen, Havva Yagci Acar, Kubra Onbasli
Summary: Development of targeted nanoparticles as carriers to deliver photosensitizers to cancer cells is highly beneficial for ensuring the expected therapeutic outcome of photodynamic therapy. In the study, Cet-PAA@SPION/BOD-Se-I showed significantly enhanced phototoxicity on colon cancer cells and completely eliminated phototoxicity on healthy cells.
Article
Chemistry, Multidisciplinary
Parisa Sharif, Eda Alemdar, Soner Ozturk, Omer Caylan, Tugba Haciefendioglu, Goknur Buke, Murat Aydemir, Andrew Danos, Andrew P. Monkman, Erol Yildirim, Gorkem Gunbas, Ali Cirpan, Ahmet Oral
Summary: Observation of thermally activated delayed fluorescence (TADF) has revolutionized the molecular design of highly efficient OLEDs. The effectiveness of TADF is largely determined by the first-order mixing coefficient (lambda) and the difference between singlet and triplet excited state energies (Delta E-ST). While Delta E-ST has been the main focus, the influence of spin-orbit coupling (H-SO) has been overlooked. In this study, TADF materials with increased H-SO are achieved by substituting heteroatoms with heavy-atom selenium, resulting in remarkable EQEs.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Article
Chemistry, Multidisciplinary
Ahmet Caglar Ozketen, Osman Karaman, Alara Ozdemir, Isil Soysal, Caglayan Kizilenis, Aisegkioul Nteli Chatzioglou, Yagiz Anil Cicek, Safacan Kolemen, Gorkem Gunbas
Summary: Microorganisms play a crucial role in human survival, but they can also cause infections. Antibiotics are important for controlling the balance between microorganisms. However, the emergence of multidrug-resistant bacteria poses a threat to health, and the discovery of new antibiotics is a slow process. Cationic photosensitizers offer a unique approach to develop light-inducible antimicrobial drugs.
Review
Chemistry, Inorganic & Nuclear
Osman Karaman, Gizem Atakan Alkan, Caglayan Kizilenis, Cevahir Ceren Akgul, Gorkem Gunbas
Summary: This article reviews the design and synthesis strategies, key photophysical properties, application in fluorescence imaging, and limited utilization as photodynamic therapy agents of hybrid xanthene dyes that have emerged in the last decade.
COORDINATION CHEMISTRY REVIEWS
(2023)
Article
Chemistry, Applied
Musa Dirak, Dilay Kepil, Toghrul Almammadov, Zubeyir Elmazoglu, Sultan Cetin, Naz Ozogul, Gorkem Gunbas, Safacan Kolemen
Summary: A hydrogen sulfide (H2S) responsive iodinated resorufin-based photosensitizer (RHS) was developed for selective treatment of neuroblastoma cancer cells using photodynamic therapy (PDT). RHS showed fluorescence signal and singlet oxygen generation capability after reacting with H2S, demonstrating its potential as a phototheranostic agent. It exhibited high cytotoxicity in H2S rich neuroblastoma cells but not in H2S deficient nonmalignant fibroblast cells. RHS is the first example of a H2S activatable photo-theranostic agent based on resorufin, providing a new pathway for effective neuroblastoma treatment through PDT.
Article
Chemistry, Physical
Yuying Song, Scott Svadlenak, Tanmayi Bathena, Melanie J. Hazlett, William S. Epling, Konstantinos A. Goulas, Lars C. Grabow
Summary: Designing robust catalysts for low-temperature oxidation is crucial for the development of advanced combustion engines. Through computational screening, a PdCu alloy catalyst was discovered that can completely oxidize CO below 150 degrees C without inhibition by NO, propylene, or water. This catalyst can be used together with a PtPd catalyst in a two-bed system to achieve sequential, inhibition-free complete oxidation of CO and 50% NO conversion below 120 degrees C.
Article
Chemistry, Analytical
Aliekber Karabag, Dilek Soyler, Yasemin Arslan Udum, Levent Toppare, Gorkem Gunbas, Saniye Soylemez
Summary: Molecular engineering of conjugated systems is an effective approach for understanding structure-property relationships in the development of optoelectronic properties and biosensing characteristics. In this study, a series of conjugated monomers based on thieno[3,4-c]pyrrole-4,6-dione (TPD), modified with electron-rich selenophene, 3,4-ethylenedioxythiophene (EDOT), or both, were synthesized and their electrochromic properties and applications in glucose biosensing were investigated. The results showed that structural modifications significantly influenced the electrochemical, electronic, optical, and biosensing properties of the materials.
Article
Chemistry, Physical
Stephen Kristy, Scott Svadlenak, Adam S. Hoffman, Simon R. Bare, Konstantinos A. Goulas
Summary: This study reports the effects of alloying Cu into silica supported Pd nanoparticles on the catalytic activity, surface composition, and particle structure during CO oxidation. By alloying Cu into Pd nanoparticles, the inhibition of CO oxidation by NO is suppressed without any loss of CO oxidation activity.
APPLIED CATALYSIS B-ENVIRONMENT AND ENERGY
(2024)
Article
Nanoscience & Nanotechnology
Toghrul Almammadov, Zubeyir Elmazoglu, Gizem Atakan, Dilay Kepil, Guzide Aykent, Safacan Kolemen, Gorkem Gunbas
Summary: Selective and effective therapy for brain cancer, particularly glioblastoma, remains challenging. In this study, a new photodynamic therapy agent was developed, which could selectively kill glioblastoma cells and emit fluorescence upon enzymatic activation.
ACS APPLIED BIO MATERIALS
(2022)
Article
Nanoscience & Nanotechnology
Eda Kilic, Zubeyir Elmazoglu, Toghrul Almammadov, Dilay Kepil, Thibaud Etienne, Antoine Marion, Gorkem Gunbas, Safacan Kolemen
Summary: In this study, photosensitizers with a heavy iodine atom were developed, which can be further converted to activity-based photosensitizers. Cell culture experiments proved the potential application of these photosensitizers in phototherapy and diagnosis.
ACS APPLIED BIO MATERIALS
(2022)
Article
Chemistry, Organic
Siddhartha Pal, Pramod S. Kumbhar, Anjani Varma, Sasisanker Padmanabhan
Summary: This review discusses the importance of efficient fractionation and downstream process integration for the implementation and viability of lignocellulosic biorefineries in producing biofuels and bio-based chemicals. It highlights the significance of pre-treatment, chemical additives, and technology development in enhancing fermentable sugar production. Additionally, the review covers various pre-treatment methods and emphasizes the transition from lab scale to demo/commercial scale for scalability and viability of bioethanol technology.
TRENDS IN CARBOHYDRATE RESEARCH
(2021)
