Article
Multidisciplinary Sciences
Peter J. Dahl, Sophia M. Yi, Yangqi Gu, Atanu Acharya, Catharine Shipps, Jens Neu, J. Patrick O'Brien, Uriel N. Morzan, Subhajyoti Chaudhuri, Matthew J. Guberman-Pfeffer, Dennis Vu, Sibel Ebru Yalcin, Victor S. Batista, Nikhil S. Malvankar
Summary: Geobacter sulfurreducens transports respiratory electrons over micrometers through nanowires composed of polymerized cytochrome OmcS. Individual nanowires exhibit hopping conductance and show a significant increase in their intrinsic conductance upon cooling. The cooling effect is caused by the rearrangement of hydrogen bonding networks in the nanowires, resulting in a temperature-sensitive switch for charge transport controlled by the protein surrounding the hemes.
Article
Biotechnology & Applied Microbiology
Lewis Grozinger, Elizabeth Heidrich, Angel Goni-Moreno
Summary: Synthetic biology utilizes molecular biology to create genetic circuits that can perform computations. These circuits process inputs and produce outputs based on predefined rules encoded in genetic parts. However, recent advancements in the field have shifted towards using mechanisms beyond genetic parts for engineering biological circuits. This study explores the use of electrogenic processes for circuit design and presents a model of a hybrid genetic and electrogenetic toggle switch operating in a biofilm attached to an electrode. Computational simulations were conducted to identify circuit design principles that result in bistability, providing a foundation for the rational design and implementation of hybrid devices that can be controlled genetically and electronically.
MICROBIAL BIOTECHNOLOGY
(2023)
Review
Biochemical Research Methods
Elie Desmond-Le Quemener, Roman Moscoviz, Nicolas Bernet, Andrew Marcus
Summary: The study of mathematical modeling of DIET and MIET in anaerobic ecosystems offers new strategies for improving biotechnologies. While the modeling of DIET kinetics is still in its early stages, recent concepts introduced for modeling electroactive biofilms should be further developed. DIET shows potential to increase IET rates compared to H 2 -MIET, but additional characterizations of biological components supporting IET are needed to enhance predictions.
CURRENT OPINION IN BIOTECHNOLOGY
(2021)
Article
Biology
Fengbin Wang, Chi Ho Chan, Victor Suciu, Khawla Mustafa, Madeline Ammend, Dong Si, Allon Hochbaum, Edward H. Egelman, Daniel R. Bond
Summary: Early genetic and structural observations suggested that metal-reducing organisms like Geobacter had extracellular conductive filaments composed of type IV pili. However, recent research has shown that bacterial c-type cytochromes can also form extracellular filaments for long-range electron transport. Cryo-electron microscopy was used to determine the structure of a third extracellular filament, formed from Geobacter sulfurreducens octaheme cytochrome, OmcZ. The structure of OmcZ differs from that of OmcS and OmcE, indicating that conductive cytochrome polymers likely arose independently from different ancestral multiheme proteins.
Article
Agricultural Engineering
Haiquan Dong, Jun Cheng, Hui Li, Liangchen Yue, Rongxin Xia, Junhu Zhou
Summary: This study investigated the use of co-culturing Geobacter sulfurreducens with mixed methanogens to address the issue of poor utilization of H-2 and CO2 in biomethane conversion. The results showed that, with a specific inoculation volume ratio, there was a significant improvement in methane production and H2 conversion efficiency. Furthermore, electrochemical detection and fluorescence spectra analysis revealed an enhanced metabolic capacity and the ability for direct interspecific electron transfer to produce CH4 in the co-culture system. Additionally, gene sequencing demonstrated the establishment of a mutualistic relationship between G. sulfurreducens and Methanoculleus.
BIORESOURCE TECHNOLOGY
(2022)
Article
Chemistry, Analytical
R. Saravanakumar, P. Pirabaharan, L. Rajendran
Summary: The motivation of this work was to develop a theoretical analysis for the transport and kinetics of substrate and product in PPO-based rotating disk bioelectrodes. The model is based on a nonlinear reaction-diffusion system containing a nonlinear term related to the CEC' mechanism. The hyperbolic function approach was used to solve the nonlinear coupled system of reaction-diffusion equations. Approximate analytical expressions for phenol, catechol, o-quinone concentrations, and current response were obtained for various diffusion and kinetic parameter values. The sensitivity and amplification factor of the bioelectrode towards phenol substrate were also studied in terms of rotation rate and enzyme layer thickness. Numerical solutions were obtained using Scilab/Matlab program, and a good agreement was observed between analytical and numerical results.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2022)
Article
Environmental Sciences
Zheng Zhuang, Guiqin Yang, Li Zhuang
Summary: The study investigated the role of exopolysaccharides in electroactive biofilms by constructing an exopolysaccharides-deficient Geobacter sulfurreducens-based BES. The findings demonstrated the essential role of exopolysaccharides in extracellular electron transfer (EET) and early biofilm formation, with pili playing a compensatory role in biofilm formation. The mutant biofilm had lower cell viability, thinner biofilm, and decreased electrochemical activity compared to the wild-type biofilm.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Biochemistry & Molecular Biology
Dietmar Hammerschmid, Francesca Germani, Salvador I. Drusin, Charline Fagnen, Claudio D. Schuster, David Hoogewijs, Marcelo A. Marti, Catherine Venien-Bryan, Luc Moens, Sabine Van Doorslaer, Frank Sobott, Sylvia Dewilde
Summary: GCS, consisting of a sensor/globin, linker, and transmitter domain, have different activities and functions depending on the nature of the transmitter domain. The study characterized GsGCS spectroscopically, showing it forms a tetramer with minimal contact between the GDs and outward-oriented heme groups, making intramolecular signal transduction less likely. Results suggest a potential role for GsGCS as a redox sensor or in membrane-bound e(-)/H+ transfer.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2021)
Article
Chemistry, Physical
Xuan Liu, Mariana C. O. Monteiro, Marc T. M. Koper
Summary: Insights into the mechanism and kinetics of the electrochemical CO2 reduction reaction are limited by the lack of information on interfacial pH. This study combines a reliable pH sensor with the rotating ring-disk electrode technique to investigate the relationship between interfacial pH and CO2 reduction reactions. The results show that interfacial pH increases with increasing current density, and there is a sharp increase at around -0.5 V vs. RHE, indicating a change in buffering species. pH measurements under different conditions further reveal the influence of electrolyte effects on interfacial pH.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2023)
Article
Microbiology
Jie Jiang, Pengchen He, Ying Luo, Zhaofeng Peng, Yongguang Jiang, Yidan Hu, Lei Qi, Xiuzhu Dong, Yiran Dong, Liang Shi
Summary: The pilA-N, omcE, omcS, omcT, and omcZ genes of Geobacter sulfurreducens have different roles in extracellular electron transfer (EET), enabling efficient EET reactions with substrates of different properties.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Roland Berthomieu, Maria Fernanda Perez-Bernal, Gaelle Santa-Catalina, Elie Desmond-Le Quemener, Nicolas Bernet, Eric Trably
Summary: The study revealed the interaction mechanism between the electroactive bacterium Geobacter sulfurreducens and Clostridium pasteurianum, with Geobacter sulfurreducens potentially modifying the metabolic pathway of Clostridium pasteurianum through the production of cofactor molecules, and entering Clostridium pasteurianum with its electrons, possibly reinforcing the metabolic shift in Clostridium pasteurianum.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Microbiology
Monir Mollaei, Maria Suarez-Diez, Vicente T. Sedano-Nunez, Sjef Boeren, Alfons J. M. Stams, Caroline M. Plugge
Summary: The study suggests that the interspecies electron transfer (IET) mechanism in the SF-GS coculture may involve both direct electron transfer (DIET) and hydrogen/formate transfer (HFIT), indicating a complex interaction between the two species.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Fernanda Jimenez Otero, Grayson L. Chadwick, Matthew D. Yates, Rebecca L. Mickol, Scott H. Saunders, Sarah M. Glaven, Jeffrey A. Gralnick, Dianne K. Newman, Leonard M. Tender, Victoria J. Orphan, Daniel R. Bond
Summary: A study on a strain of Geobacter sulfurreducens lacking four of five outer membrane cytochrome complexes found that its biofilm growth is faster and produces greater current density. This increased performance is attributed to denser cell packing close to the electrode and higher metabolic rates per cell, as indicated by increased rates of N-15 incorporation. Additionally, the engineered strain shows a higher rate of electron transfer through biofilms compared to the wild type, suggesting a streamlined electron transfer pathway for enhanced current production.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Chemistry, Analytical
Milivoj Lovric
Summary: Theoretical single scan staircase voltammograms of homogeneous electrocatalytic reactions on the rotating disk electrode were calculated by digital simulation. A simple relationship between steady state current and dimensionless kinetic parameter was discovered, and the influence of the backward component of chemical reaction was demonstrated.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2022)
Article
Environmental Sciences
Huihui Zhou, Xianwen Xuanyuan, Xiaowei Lv, Jing Wang, Kun Feng, Chuan Chen, Jun Ma, Defeng Xing
Summary: Electroactive bacteria such as Geobacter sulfurreducens show plasticity in their response to magnetic fields, making them promising candidates for magnetic sensor applications. This study investigated the effects of magnetic fields on current generation by G. sulfurreducens and identified the molecular mechanism underlying this response at the transcriptomic level. Magnetic field treatment significantly enhanced electricity generation and energy efficiency of G. sulfurreducens. The response of current to magnetic fields was instantaneous and reversible. Differential gene expression analysis revealed upregulation of genes associated with electron transfer in G. sulfurreducens under magnetic field treatment.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Chemistry, Multidisciplinary
Paulo R. Bueno, German D. Schrott, Pablo S. Bonanni, Silvia N. Simison, Juan P. Busalmen
Article
Chemistry, Multidisciplinary
Diego Massazza, Rodrigo Parra, Juan P. Busalmen, Hernan E. Romeo
ENERGY & ENVIRONMENTAL SCIENCE
(2015)
Editorial Material
Chemistry, Multidisciplinary
M. V. Ordonez, G. D. Schrott, D. A. Massazza, J. P. Busalmen
ENERGY & ENVIRONMENTAL SCIENCE
(2016)
Article
Chemistry, Analytical
Luciana Robuschi, J. Pablo Tomba, Juan Pablo Busalmen
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2017)
Article
Environmental Sciences
Tobias Salinas, Ignacio Durruty, Lorena Arciniegas, Gustavo Pasquevich, Magas Lanfranconi, Isabela Orsi, Vera Alvarez, Sebastian Bonanni
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2018)
Article
Chemistry, Physical
Diego Massazza, Juan P. Busalmen, Rodrigo Parra, Hernan E. Romeo
JOURNAL OF MATERIALS CHEMISTRY A
(2018)
Article
Biochemistry & Molecular Biology
Beatriz Maestro, Juan M. Ortiz, German Schrott, Juan P. Busalmen, Victor Climent, Juan M. Feliu
BIOELECTROCHEMISTRY
(2014)
Article
Electrochemistry
German D. Schrott, P. Sebastian Bonanni, Juan Pablo Busalmen
ELECTROCHIMICA ACTA
(2019)
Article
Engineering, Environmental
S. Cabred, V Giunta Ramos, J. E. Busalmen, J. P. Busalmen, S. Bonanni
CHEMICAL ENGINEERING JOURNAL
(2019)
Article
Chemistry, Multidisciplinary
Maria Victoria Ordonez, Luciana Robuschi, Cristina Elena Hoppe, Juan Pablo Busalmen
ENVIRONMENTAL SCIENCE-NANO
(2020)