Article
Chemistry, Analytical
Xianzhe Wei, Tong Zhu, Yongsheng Ma, Jianying Sun, Gengxiu Zheng, Tingbin Ma, Xiaofeng Yang, Zhiling Song, Yanfeng Lv, Jing Zhang, Mei Yan
Summary: Oxidative stress plays a significant role in various diseases, and acetylcholinesterase (AChE) plays a key role in regulating oxidative stress. However, there is a lack of effective analytical method for real-time monitoring of AChE under oxidative stress. This study designed and synthesized two NIR fluorescent probes (SNCN-AE and SNC-AE) for accurate detection of AChE activity by combining ESIPT and ICT processes. These probes showed distinct NIR fluorescence signals and large Stokes shifts, and SNCN-AE exhibited better detection performance and low cytotoxicity, making it suitable for imaging endogenous AChE in living systems. Importantly, SNCN-AE was successfully applied for monitoring AChE under oxidative stress in PC12 cells and zebrafish model, making it a promising tool for studying oxidative stress and related diseases.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Applied
Wenyi Liao, Shuwei Zhang, Xuewen Wang, Yuyuan Sun, Ting Wang, Huaqian Xu, Yu Yuan, Gang Chen, Xiaodong Jia
Summary: The abnormality of Cys concentration in organism could lead to a series of diseases, so its effective detection is of great importance in disease diagnosis. Here we developed a novel ESIPT fluorescent probe HBT-IDAc based on the classic ESIPT fluorophore 2-(2'-hydroxyphenyl)benzothiazole (HBT) and indanone, which could selectively and sensitively detect Cys with a large Stokes shift up to 210 nm and the detection limit to 0.89 μM. In addition, the probe exhibited good anti-interference and low cytotoxicity, and has been successfully applied to monitor Cys in cells.
Article
Chemistry, Applied
Jin Yu, Wei Shu, Hao Kang, Rubing Han, Xiaoli Zhang, Rubo Zhang, Jing Jing, Xiaoling Zhang
Summary: The quick reaction of nitric oxide with superoxide free radicals produces peroxynitrite, a powerful biological oxidant that leads to various diseases by interacting with proteins, lipids, nucleic acids, and other organisms. In this study, a fluorescent probe named YV was developed for the precise and sensitive detection of endogenous peroxynitrite. The probe exhibited high selectivity, fast response, significant Stokes shift, and low detection limit, making it a robust imaging tool for identifying variations in peroxynitrite.
Article
Chemistry, Analytical
Hao Chang, Yu Mei, Yidan Li, Luqing Shang
Summary: In this study, a novel neuraminidase fluorescent probe with aggregation-induced emission and excited state intramolecular proton transfer activity was developed for influenza virus detection. The probe showed high sensitivity and strong fluorescence signal towards neuraminidase, making it suitable for influenza virus detection and living cell imaging. It was also able to distinguish oseltamivir-resistant influenza virus from wild type.
Article
Spectroscopy
Hai-Yan Peng, Gang Zhang, Ru Sun, Yu-Jie Xu, Jian-Feng Ge
Summary: In order to detect the level of thiophenols in the environment and biological samples, probes 1a-b were synthesized by introducing the 2,4-dinitrophenyl ether group into diethylcoumarin-salicylaldehyde based compounds. These probes can form host-guest compounds with methylated beta-cyclodextrin, showing significant increase in fluorescence intensity in thiophenols detection. The hydrophobic cavity of methylated beta-cyclodextrin enhances the fluorescence intensity of probes 1a-b and reduces their detection limits to thiophenols, without affecting their selectivity and response time.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Chemistry, Applied
Tingting Zhu, Yan Hu, Xi Chen, Haibing Shao, Zhihua Chen, Hua Zhang, Chuanxiang Liu
Summary: A novel chromene-derived fluorescent probe was developed with a unique ESIPT mechanism for sensing cyanides in aqueous environments with high selectivity and sensitivity. The probe's structure changes under cyanide induction, and test strips based on this probe were successfully fabricated for practical cyanide detection. This probe also shows great potential for cyanide detection in food samples such as cassava flour and bitter almonds.
Article
Spectroscopy
Yulong Liu, Yeqi Zhang, Ming Sheng, Yihan Kang, Binbin Jia, Wenbo Li, Ying Fu
Summary: Based on the classical Schiff base reaction, a fluorescent probe PAI was designed and synthesized through structural modification by introducing Schiff base structure to pyrene unit. The synthesized PAI was characterized by FT-IR, 1H NMR, 13C NMR, and HRMS. PAI is a turn-on probe with high selectivity for specific recognition of Al3+ ion, and has a lower limit of detection compared to many other reported probes. The addition of Al3+ ion breaks the carbon-nitrogen double bond of Schiff base in PAI, resulting in a product without PET property and exhibiting enhanced fluorescence and blue color. PAI can also recognize Al3+ ion through test papers, which is beneficial for future research on Al3+ ion sensing.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Spectroscopy
Fugang Fan, Chenyang Xu, Xina Liu, Meiqing Zhu, Yi Wang
Summary: A novel fluorescent probe, BRBA, has been developed for selective detection of hydrazine under complex conditions. The probe exhibits low cytotoxicity and a large Stokes shift, making it suitable for monitoring environmental water samples and imaging cells.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Biochemical Research Methods
Shengli Hu, Junyu Wang, Meiling Luo, Zhou Wu, Yaoyao Hou, Xinpeng Chen
Summary: A new fluorescent probe derived from 3-hydroxyphthalimide was synthesized, which can rapidly and selectively detect hydrazine with potential applications in monitoring hydrazine in the environment and living cells.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Chunwei Yu, Mei Yang, Shuhua Cui, Yuxiang Ji, Jun Zhang
Summary: A novel ratiometric Cu2+-selective probe was constructed based on pyrene derivative, which showed selective recognition and detection of Cu2+ through a turn-on fluorescent response. The formation of a 1:1 metal-ligand complex and the binding mode were confirmed by MS spectra, Job's plot experiment, and H-1 NMR experiment. Satisfactory results were obtained from fluorescence imaging in living cells for Cu2+ detection.
Article
Spectroscopy
Feng Li, Chang-He Tian, Ya-Fei Du, Bao-Xiang Zhao
Summary: A benzothiazole-based fluorescent probe was developed for the detection of benzenethiol derivatives, showing rapid response and low detection limit, with potential for detecting such derivatives in real water samples.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Spectroscopy
Yu Wang, Qi Yan, Zhonglong Wang, Haijun Xu
Summary: In this study, a flavonol-derived fluorescent probe named TB-N2H4 was developed for detecting N2H4 based on the excited intramolecular proton transfer (ESIPT) principle. TB-N2H4 exhibited a remarkable fluorescence turn-on response toward N2H4 with a large Stokes shift. It could selectively recognize N2H4 and showed high sensitivity and low detection limit, making it suitable for quantifying N2H4 in environmental samples and tracking its distribution in living organisms.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Chemistry, Analytical
Liqiang Yan, Hong Yang, Jia Li, Cuiping Zhou, Lin Li, Xiongzhi Wu, Chenghong Lei
Summary: The fluorescent probe prepared in this study can rapidly and effectively detect zinc ions and hypochloric acid, showing good optical properties for cell imaging and satisfactory recoveries in actual water samples.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Ying Hu, Lingmin Lu, Shiman Guo, Xiaolan Wu, Jiayi Zhang, Chunsong Zhou, Haiyan Fu, Yuanbin She
Summary: A reversible fluorescent probe Pyr-1 was developed for visual detection of Cysteine (Cys) by integrating pyrene and oxime functional groups. Pyr-1 exhibited selective fluorescence quenching response towards Fe3+ and the ensemble Pyr-1-Fe3 complex was applied for Cys detection. Cys induced unique fluorescence enhancement through the decomplexation process.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Yan Niu, Haoping Wang, Yunxia Wang, Liheng Feng
Summary: The designed fluorescent probe TC-gal shows high selectivity and ultra-high sensitivity for detecting beta-galactosidase, with strong anti-interference ability to other biological analytes. By adjusting the intramolecular charge transfer effect, the probe achieves specific turn-on fluorescence triggered by beta-galactosidase.
MICROCHEMICAL JOURNAL
(2021)
Article
Chemistry, Organic
Tobias Rautio, Jonathan Thornell, Henrik Green, Peter Konradsson, Johan Dahlen, Xiongyu Wu
Summary: Fentanyl analogues have become a serious issue in the recreational drug market due to their high potencies and fatal overdoses. Researchers have developed a concise synthetic route for the synthesis of metabolites of seven fentanyl analogues, without the need for silica gel purification.
SYNTHETIC COMMUNICATIONS
(2022)
Article
Chemistry, Physical
Xin Zhang, Juan Chen, Jiwen Hu, Anna du Rietz, Xiongyu Wu, Ruilong Zhang, Zhongping Zhang, Kajsa Uvdal, Zhangjun Hu
Summary: Mitophagy plays a critical role in maintaining cellular homeostasis by allowing acidic lysosomes to engulf excess or impaired mitochondria. Tracking pH fluctuations is essential for studying mitophagy, and this study developed a tailored fluorogenic probe with mitochondrial specificity, excellent biocompatibility, wide pH-sensitive range, and quantitative ability. The probe integrated multiple functionalities into a single molecule, making it suitable for real-time tracking of pH fluctuations in mitophagy. This work provides valuable insights for the fabrication of advanced fluorescent nanoprobes for various biomedical applications.
Article
Chemistry, Multidisciplinary
Katriann Arja, Robert Selegard, Marketa Paloncyova, Mathieu Linares, Mikael Lindgren, Patrick Norman, Daniel Aili, K. Peter R. Nilsson
Summary: This study reports on the chiro-optical properties and architectural arrangement of optoelectronic materials generated through self-assembly of oligothiophene-porphyrin derivatives and synthetic peptides. The materials exhibit a three-dimensional ordered helical structure and optical activity, making them promising for bioelectronic applications.
Editorial Material
Chemistry, Organic
Henrik Ottosson, Bo Durbeej, Miquel Sola
JOURNAL OF PHYSICAL ORGANIC CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Afshan Begum, Jun Zhang, Dean Derbyshire, Xiongyu Wu, Peter Konradsson, Per Hammarstroem, Eleonore von Castelmur
Summary: This study reports two trans-stilbene analog ligands that bind to native tetrameric TTR. Crystal structures revealed that the binding modes of these ligands were opposite to the predictions. One binding mode resulted in pH sensitivity of the fluorescent ligand, while the other mediated a stabilizing conformational change. These structures provide important insights into ligand design and interpretation of trans-stilbene binding modes to the TTR T4 binding site.
ACS CHEMICAL NEUROSCIENCE
(2023)
Review
Biochemistry & Molecular Biology
Per Hammarstroem, Sofie Nystroem
Summary: The interaction between viral infections, amyloid formation, and neurodegeneration has been a topic of discussion for a long time. Viral proteins can form amyloids, and post-acute sequalae (PAS) after viral infections have been observed. SARS-CoV-2 and COVID-19 have shown a connection between amyloid formation and severe outcomes in the acute infection, PAS, and neurodegenerative diseases. Various intersecting aspects of viruses and amyloids are highlighted, including evolutionary driving forces, protein endoproteolysis, and co-operativity between amyloids and viruses. The abnormal blood clotting in severe and long COVID, as well as the side effects of some vaccines, is linked to amyloid formation. Antiviral drug development, implementation, and the identification of suitable antigen targets for future vaccines are emphasized.
Article
Chemistry, Physical
Victoria M. Bjelland, Harue Nakashima, Naoaki Hashimoto, Satoshi Seo, Thor B. Melo, Mikael Lindgren
Summary: This study examines the molecular processes of triplet-triplet annihilation (TTA) in solvents at room temperature, with special focus on nonlinear kinetic reactions. Time- and spectrally resolved spectroscopy is used to simulate, measure, and fit the kinetic parameters of TET and TTA rate reactions without approximations. The study reveals that viscosity/diffusion and the excited triplet lifetime of the annihilator are crucial parameters for high TTA induced delayed fluorescence.
Article
Chemistry, Physical
Ingvild U. Hageberg, Katrianne Arja, Benedikte E. Vindstad, Johannes K. Bergvoll, Odrun A. Gederaas, Thor-Bernt Melo, K. Peter R. Nilsson, Mikael Lindgren
Summary: We studied a new synthetic method, photophysical properties, and photosensitizing effects of glycosylated 4,5,6,7-tetrahydropyrazolo[1,5-a]pyridine] fused chlorin agents. The glycochlorins showed superior biological activity compared to the unglycosylated chlorin, with the proto form being more effective than the Zn-stabilized variant. Cell studies indicated that the photosensitizer localized in the mitochondria, cell membrane, and lysosomes, with dominance in the lysosomes.
Article
Chemistry, Multidisciplinary
Enrique M. Arpa, Sven Stafstrom, Bo Durbeej
Summary: This study proposes a simple design for achieving unidirectional photogearing through a stereochemical approach, which has been successfully tested using quantum-chemical modeling. The study provides new insights for the through-space transmission of the double-bond rotary motion of light-driven molecular motors.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Chemistry, Analytical
Steven R. Baginski, Tobias Rautio, Lorna A. Nisbet, Karin Lindbom, Xiongyu Wu, Johan Dahlen, Craig Mckenzie, Henrik Green
Summary: Understanding the metabolism of synthetic cannabinoid receptor agonists (SCRAs) is crucial for clinical and forensic toxicology. In this study, the metabolism of ADB-HEXINACA was investigated using in vitro techniques, leading to the discovery of new biomarkers for its consumption.
JOURNAL OF ANALYTICAL TOXICOLOGY
(2023)
Article
Chemistry, Physical
Nghia Nguyen Thi Minh, Afshan Begum, Jun Zhang, Petter Leira, Yogesh Todarwal, Mathieu Linares, Patrick Norman, Dean Derbyshire, Eleonore von Castelmur, Mikael Lindgren, Per Hammarstroem, Carolin Koenig
Summary: Misfolding and aggregation of transthyretin (TTR) can lead to various amyloid diseases. TTR not only acts as an amyloidogenic protein but also has an affinity for small-molecule ligands in its thyroxine (T4) binding site. In this study, we investigated the binding of a fluorescent amyloid ligand, Py1SA, to tetrameric TTR using spectroscopic evidence and X-ray crystallography. Although the X-ray diffraction data were inconclusive, molecular dynamics simulations and umbrella sampling approaches were used to determine the preferred orientation of the ligand in the T4 binding site. Our findings provide insights into the binding mode preference of TTR for trans-stilbene salicylic acid derivatives and a framework for determining structures of TTR-ligand complexes.
JOURNAL OF PHYSICAL CHEMISTRY B
(2023)
Article
Chemistry, Physical
Javier Corpas, Miguel Gomez-Mendoza, Enrique M. Arpa, Victor A. de la Pena O'Shea, Bo Durbeej, Juan C. Carretero, Pablo Mauleon, Ramon Gomez Arrayas
Summary: This article demonstrates the synthesis of stereodivergent tetrasubstituted beta-boryl acrylates through iterative dual-metal and energy transfer catalysis. The reluctance of electron-deficient internal alkynes to undergo catalytic carboboration has been overcome through cooperative Cu/Pd-catalysis, while an Ir complex acts as a versatile sensitizer for photoisomerization of the resulting sterically crowded alkenes.
Article
Chemistry, Physical
Enrique M. Arpa, Bo Durbeej
Summary: Excited-state aromaticity (ESA) and antiaromaticity (ESAA) are well-established concepts in explaining the photophysical properties and reactivities of cyclic, conjugated molecules. In this study, a new parameterization of the harmonic oscillator model of aromaticity (HOMA) called HOMER is presented for the T-1 state of carbocyclic and heterocyclic compounds. The results demonstrate that HOMER provides a superior description of ESA and ESAA compared to the original HOMA scheme, and can be used for predictive modeling of ESA and ESAA at different levels of theory.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2023)
Article
Chemistry, Multidisciplinary
Daniel Bosch, Jun Wang, Lluis Blancafort
Summary: This study successfully models the stability and optical absorption of Eumelanin using deep neural networks, addressing the structural challenge of Eumelanin.