期刊
CHEMPHYSCHEM
卷 14, 期 13, 页码 3138-3145出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cphc.201300436
关键词
longitudinal relaxation; molecular mobility; real-time NMR; T-1; ultrafast 2D NMR
资金
- Fulbright Foundation
- Agilent Technologies [2305]
- ERC [246754]
- EU'S BioNMR [261863]
- Perlman Family Foundation
- European Research Council (ERC) [246754] Funding Source: European Research Council (ERC)
The longitudinal relaxation properties of NMR active nuclei carry useful information about the site-specific chemical environments and about the mobility of molecular fragments. Molecular mobility is in turn a key parameter reporting both on stable properties, such as size, as well as on dynamic ones, such as transient interactions and irreversible aggregation. In order to fully investigate the latter, a fast sampling of the relaxation parameters of transiently formed molecular species may be needed. Nevertheless, the acquisition of longitudinal relaxation data is typically slow, being limited by the requirement that the time for which the nucleus relaxes be varied incrementally until a complete build-up curve is generated. Recently, a number of single-shot-inversion-recovery methods have been developed capable of alleviating this need; still, these may be challenged by either spectral resolution restrictions or when coping with very fast relaxing nuclei. Here, we present a new experiment to measure the T(1)s of multiple nuclear spins that experience fast longitudinal relaxation, while retaining full high-resolution chemical shift information. Good agreement is observed between T(1)s measured with conventional means and T(1)s measured using the new technique. The method is applied to the real-time investigation of the reaction between D-xylose and sodium borate, which is in turn elucidated with the aid of ancillary ultrafast and conventional 2D TOCSY measurements.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据