Article
Chemistry, Multidisciplinary
Satyam Khanal, Roberto J. Brea, Michael D. Burkart, Neal K. Devaraj
Summary: The study introduces a novel chemoenzymatic strategy for synthesizing lipid membranes in vitro, successfully transforming simple metabolic building blocks into lipid membranes.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Chemistry, Multidisciplinary
Anton Joseph, Anna M. Wagner, Manuela Garay-Sarmiento, Mina Aleksanyan, Tamas Haraszti, Dominik Soeder, Vasil N. Georgiev, Rumiana Dimova, Virgil Percec, Cesar Rodriguez-Emmenegger
Summary: In this study, a new type of Janus dendrimer is introduced, which can self-assemble into a mimetic cell membrane structure that closely resembles the characteristics of a natural cell membrane in terms of thickness, flexibility, and fluidity. This mimetic cell membrane exhibits resilience to harsh conditions and faster dynamics in closing pores in the event of membrane rupture. It can be used to create hybrid structures with components of natural cell membranes and has the potential to simulate life-like features such as vesicle fusion, motility, and environmental sensing.
ADVANCED MATERIALS
(2022)
Article
Multidisciplinary Sciences
Sarah B. Nyenhuis, Xufeng Wu, Marie-Paule Strub, Yang-In Yim, Abigail E. Stanton, Valentina Baena, Zulfeqhar A. Syed, Bertram Canagarajah, John A. Hammer, Jenny E. Hinshaw
Summary: It has been found that dominant optic atrophy, a leading cause of childhood blindness, is caused by gene mutations in OPA1. OPA1 encodes a protein that plays a key role in mitochondrial membrane fusion and remodelling. Using cryo-electron microscopy, researchers have been able to solve the helical structures of OPA1 on lipid membrane tubes and have discovered the importance of nucleotide in the dimerization of the GTPase domains. The study also revealed unique secondary structures in OPA1 that enhance its association with membranes. These findings provide insight into the effects of pathogenic mutations on protein folding, inter-protein assembly, and membrane interactions.
Article
Biophysics
Katherine N. Liu, Steven G. Boxer
Summary: The study introduces a new method to study the content-mixing process of viruses within single cells by binding content-labeled vesicles to unlabeled influenza virus. Results show that the cholesterol content of the target membrane can increase the efficiency of viral content mixing and decrease the proportion of events resulting in content loss. This approach may be promising for studying the single-virus content-mixing kinetics of other enveloped viruses.
BIOPHYSICAL JOURNAL
(2021)
Article
Biology
Abdelmoneim Eshra, Hartmut Schmidt, Jens Eilers, Stefan Hallermann
Summary: The basal free Ca2+ concentration critically controls action potential-evoked release, indicating a high-affinity Ca2+ sensor for vesicle priming. There is a surprisingly shallow and non-saturating relationship between release rate and intracellular Ca2+ concentration up to 50 μM. The rate of vesicle replenishment during sustained elevated intracellular Ca2+ concentration exhibited little Ca2+-dependence.
Article
Multidisciplinary Sciences
Changwei Zhao, Yanjun Zhang, Yuewen Jia, Bojun Li, Wenjing Tang, Chuning Shang, Rui Mo, Pei Li, Shaomin Liu, Sui Zhang
Summary: By controlling the spatial and temporal aspects of interfacial polymerization, a nanofiltration membrane with fast permeation and high Cl-/SO42- selectivity has been constructed. This membrane demonstrates ultra-permeability and excellent ion-ion separation, making it ideal for sustainable water treatment applications such as water purification and desalination.
NATURE COMMUNICATIONS
(2023)
Article
Chemistry, Physical
Rodolphe Marie, Martin K. Rasmussen, Jonas N. Pedersen
Summary: Self-assembly of synthetic lipid vesicles via lipid membrane fusion is a versatile tool for creating biomimetic nano- and micron-sized particles. This work demonstrates DNA-induced liposome fusion in a nanofluidic trap where the reaction occurs in a 15 femtoliter volume at homogeneous mixing. The chip-based approach limits the amount of sample (down to 440 vesicles) and can be parallelized for systematic studies in synthetic biology, diagnostics, and drug delivery.
Article
Chemistry, Multidisciplinary
Mette Galsgaard Malle, Philipp M. G. Loeffler, Soren S-R Bohr, Magnus Berg Sletfjerding, Nikolaj Alexander Risgaard, Simon Bo Jensen, Min Zhang, Per Hedegard, Stefan Vogel, Nikos S. Hatzakis
Summary: In this study, a single-particle combinatorial multiplexed liposome fusion mediated by DNA is presented, enabling multistep and non-deterministic fusion of nanocontainers. Efficient and leakage-free stochastic fusion sequences were observed for arrays of target liposomes functionalized with individual DNA strands and labeled with distinct chromophores. The method allows high-throughput combinatorial multiplex screens using minimal material.
Article
Biochemistry & Molecular Biology
Ethiene Kwok, Shauna C. Otto, Patricia Khuu, Andrew P. Carpenter, Sara J. Codding, Patrick N. Reardon, Juan Vanegas, Tanushri M. Kumar, Chapman J. Kuykendall, Ryan A. Mehl, Joe Baio, Colin P. Johnson
Summary: Dysferlin, a large membrane protein, plays a crucial role in muscle function. It is associated with muscle diseases and its loss of activity leads to reduced exocytosis and abnormal intracellular Ca2+. In this study, the researchers found that the C2A domain of dysferlin binds preferentially to membranes containing PI(4,5)P2 through specific residues on its concave face. They also discovered that binding of the C2A domain to membranes leads to changes in lipid packing and insertion of certain residues into the membrane. These findings provide insights into the mechanism of dysferlin and its role in membrane binding and Ca2+ regulation.
JOURNAL OF MOLECULAR BIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Daniela Cortes-Galvez, John A. A. Dangerfield, Christoph Metzner
Summary: Cells produce small vesicles called exosomes which have similarities to enveloped virus particles. This review discusses the similarities and differences between exosomes and enveloped viral particles, with a focus on events happening at the vesicle or virus membrane. Understanding these similarities and differences is important for basic biology as well as potential research and medical applications.
Article
Chemistry, Multidisciplinary
Yueyue Zhang, Jiangbo Liu, Xiuhai Mao, Hongxuan Fan, Fan Li, Shaopeng Wang, Jiang Li, Min Li, Xiaolei Zuo
Summary: This study successfully reconstructed vesicle assemblies that mimic vesicle clusters in living cells using synthetic vesicles and DNA nanorulers, and monitored them using a carbon fiber nanoelectrode. The results showed that the exocytosis of vesicle clusters is critically dependent on the size of clusters.
ADVANCED MATERIALS
(2023)
Article
Cell Biology
Prabhodh S. Abbineni, Joseph S. Briguglio, Edwin R. Chapman, Ronald W. Holz, Daniel Axelrod
Summary: This study measured the mobility of membrane proteins VAMP2, Syt1, and Syt7 in granule membranes. The results showed that VAMP2 and Syt7 have similar diffusion coefficients of about 3 x 10(-10) cm(2)/s, indicating their mobility. Syt1, on the other hand, had mobility below the detection limit. The study also estimated the time required for these proteins to arrive at docking and nascent fusion sites to be several tens of milliseconds.
MOLECULAR BIOLOGY OF THE CELL
(2022)
Article
Chemistry, Multidisciplinary
Benjamin Krugmann, Alexandros Koutsioubas, Luman Haris, Samantha Micciulla, Didier Lairez, Aurel Radulescu, Stephan Foerster, Andreas M. Stadler
Summary: The study investigates the adhesion process of large unilamellar vesicles with a supported membrane bilayer coated with myelin basic protein using time-resolved neutron reflectometry. The research aims to mimic and study myelination processes in different membrane compositions, showing that the adhesion energy of native membrane per MBP is higher than that of the EAE modified membrane type. This could potentially contribute to understanding myelination and remyelination processes for treating demyelinating diseases like MS.
FRONTIERS IN CHEMISTRY
(2021)
Article
Nanoscience & Nanotechnology
Ons Hmam, Felix Cote-Dubuc, Antonella Badia
Summary: By using the interfacial ionpairing association between charged phospholipid headgroups and electrochemically generated cationic ferroceniums, a single bilayer membrane can be formed on a self-assembled monolayer (SAM) chemisorbed to gold. The surface ferrocene concentration and hydrophobicity/hydrophilicity affect the formation of continuous supported lipid bilayers (SLBs).
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Physical
Grzegorz Bubak, Karina Kwapiszewska, Tomasz Kalwarczyk, Krzysztof Bielec, Tomasz Andryszewski, Michalina Iwan, Szymon Bubak, Robert Holyst
Summary: This study utilized single-molecule fluorescent correlation spectroscopies to show that the motion of inert probes with diameters ranging from 2.6 to 150 nm is mostly unobstructed in the nucleus, indicating the presence of interchromosomal channels filled with diluted protein solution. These channels allow various cargos to migrate freely at the nanoscale.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2021)
Article
Biophysics
Sophie Schobesberger, Peter Jonsson, Andrey Buzuk, Yuri Korchev, Jennifer Siggers, Julia Gorelik
BIOPHYSICAL JOURNAL
(2016)
Article
Multidisciplinary Sciences
Hariharan Subramanian, Alexander Froese, Peter Jonsson, Hannes Schmidt, Julia Gorelik, Viachesla O. Nikolaev
NATURE COMMUNICATIONS
(2018)
Article
Multidisciplinary Sciences
Victoria Junghans, Jana Hladilkova, Ana Mafalda Santos, Mikael Lund, Simon J. Davis, Peter Jonsson
SCIENTIFIC REPORTS
(2018)
Review
Immunology
Victoria Junghans, Ana Mafalda Santos, Yuan Lui, Simon J. Davis, Peter Jonsson
FRONTIERS IN IMMUNOLOGY
(2018)
Article
Chemistry, Multidisciplinary
Sing Yee Yeung, Yulia Sergeeva, Tommy Dam, Peter Jonsson, Guoqing Pan, Vivek Chaturvedi, Borje Sellergren
Article
Multidisciplinary Sciences
Ricardo A. Fernandes, Kristina A. Ganzinger, Justin C. Tzou, Peter Jonsson, Steven F. Lee, Matthieu Palayret, Ana Mafalda Santos, Alexander R. Carr, Aleks Ponjavic, Veronica T. Chang, Charlotte Macleod, B. Christoffer Lagerholm, Alan E. Lindsay, Omer Dushek, Andreas Tilevik, Simon J. Davis, David Klenerman
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2019)
Article
Multidisciplinary Sciences
Jenny Algotsson, Peter Jonsson, Jan Forsman, Daniel Topgaard, Olle Soderman
Article
Cell Biology
Victoria Junghans, Manto Chouliara, Ana Mafalda Santos, Deborah Hatherley, Jan Petersen, Tommy Dam, Lena M. Svensson, Jamie Rossjohn, Simon J. Davis, Peter Jonsson
JOURNAL OF CELL SCIENCE
(2020)
Article
Chemistry, Physical
Marco Fornasier, Andrea Porcheddu, Anna Casu, Srinivasa R. Raghavan, Peter Jonsson, Karin Schillen, Sergio Murgia
Summary: Nanoerythrosomes (NERs) are vesicle-like nanoparticles derived from red blood cells, offering high stability and excellent biocompatibility for delivering therapeutic molecules and imaging probes. Functionalization of NERs with fluorescent dyes using different methods showed promising potential for diagnostic purposes. Physicochemical characterization confirmed the stability and efficient loading of fluorophores on the NERs surface.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2021)
Article
Physiology
Tommy Dam, Victoria Junghans, Jane Humphrey, Manto Chouliara, Peter Jonsson
Summary: Nickel-chelating lipids can induce calcium signaling in T cells independently, potentially acting as ligands to the cell's TCRs. This could lead to false signaling events in cell studies with SLBs if not accounted for. Increasing the amount of nickel-chelating lipids on the SLB can enhance signaling fraction, while adding CD48-ligand does not significantly affect the signaling intensity.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Katarzyna Makasewicz, Stefan Wennmalm, Bjorn Stenqvist, Marco Fornasier, Alexandra Andersson, Peter Jonsson, Sara Linse, Emma Sparr
Summary: Cooperative binding is a crucial feature in metabolic pathways, signaling, and transport processes, allowing for tight regulation over a narrow concentration range. The study shows strong positive cooperativity of alpha-synuclein binding to phospholipid membranes, indicating that the protein has a higher affinity for a membrane where there is already bound protein, compared to a bare membrane.
ACS CHEMICAL NEUROSCIENCE
(2021)
Article
Biophysics
Manto Chouliara, Victoria Junghans, Tommy Dam, Ana Mafalda Santos, Simon J. Davis, Peter Jonsson
Summary: The study introduced a method to measure 2D affinity on single cells using polyhistidine-tagged fluorescent ligands anchored to a supported lipid bilayer. By decreasing ligand density in the SLB, a new steady-state accumulation in the contact was obtained, allowing for determination of both 2D affinity and the number of receptors on the cell. The analysis showed that cell contact size increased with ligand density and the number of receptors per cell, but remained constant when lowering ligand density above a certain threshold, indicating a heterogeneous process.
BIOPHYSICAL JOURNAL
(2021)
Review
Biochemistry & Molecular Biology
Tommy Dam, Manto Chouliara, Victoria Junghans, Peter Jonsson
Summary: Binding between protein molecules on contacting cells is essential in initiating and regulating key biological processes. The use of model membranes, such as supported lipid bilayers, allows for the study of 2D binding kinetics in cell-SLB contacts, providing valuable insights into ligand-receptor interactions in vivo.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Nanoscience & Nanotechnology
Sing Yee Yeung, Yulia Sergeeva, Guoqing Pan, Silvia Mittler, Thomas Ederth, Tommy Dam, Peter Jonsson, Zahra El-Schich, Anette Gjorloff Wingren, Adam Tillo, Sabrina Hsiung Mattisson, Bo Holmqvist, Maria M. Stollenwerk, Borje Sellergren
Summary: This study reports a supramolecular-based approach to control cell adhesion behavior by regulating surface composition. The results suggest that rSAMs featuring mobile bioactive ligands have great potential in biomaterial design, tissue engineering, and regenerative medicine.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Neurosciences
Alexandra Andersson, Marco Fornasier, Katarzyna Makasewicz, Tinna Palmadottir, Sara Linse, Emma Sparr, Peter Jonsson
Summary: Interactions among lipid vesicles are crucial in various functions and dysfunctions of the human body. This study presents a method using fluorescence microscopy and MATLAB analysis program to study protein-lipid interactions, vesicle fusion, fission, and lipid exchange. The method allows for quantitative analysis of single events and their frequency, providing valuable information for understanding the roles of lipid vesicles in healthy and degenerative pathways.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2022)