Discrete arrays of liquid-crystal-supported proteolipid monolayers as phantom cell surfaces

The phospholipid bilayers of living cell membranes exist almost universally in a liquid state. This enables motion and spatial reorganization of membrane components on multiple length scales, which is on essential feature of many biological processes., There is great interest in the development of molecularly defined. interfaces between synthetic materials and living cells. To this end, there is a need for solid substrate materials that can be derivatized with fluid, membrane-like interfaces. Herein, we describe, array fabrication of discrete liquid-crystal areas supporting phospholipid monolayer membranes, and characterize the interactions with several different membrane surface proteins [avidin series, cholera toxin, green fluorescent protein (GFP), intercellular adhesion molecule (ICAM) and major histocompability complex (MHC)]. Three different linkage strategies (biotin nickel chelating lipids complexing with histitdine, and the choletoxin binding unit (CTB) associating with G(MI) are evaluated. Additionally, experiments with live immunological, T cells forming active synapses at the interface exhibit the specific nature of the surface.