Article
Environmental Sciences
Jiawen Cui, Zhiyu Hao, Qin Zhou, Minna Qiu, Yuhang Liu, Yuhao Liu, Xiaohua Teng, Lu Kang
Summary: Water pollution caused by the widely used agricultural pesticide chlorpyrifos has led to public concern. This study investigates the effect of chlorpyrifos on the livers of common carp, revealing that it induces liver injury and mitochondrial dysfunction through the activation of autophagy and mitophagy. Additionally, chlorpyrifos disrupts energy metabolism, activates oxidative stress, and causes time-dependent hepatotoxicity. These findings provide insights into the molecular mechanism of chlorpyrifos-induced hepatotoxicity and contribute to the evaluation of pesticide toxicity in aquatic organisms.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Chemistry, Multidisciplinary
Byoung-Hoon Kang, Youngseop Lee, Eun-Sil Yu, Hamin Na, Minhee Kang, Hee Jae Huh, Ki-Hun Jeong
Summary: The rapid and accurate molecular diagnostics are crucial in the face of pandemic diseases, and the nanoplasmonic PCR provides a new solution for rapid and quantitative molecular diagnostics at point-of-care level.
Article
Chemistry, Multidisciplinary
Jing Tu, Yi Qiao, Yuhan Luo, Naiyun Long, Zuhong Lu
Summary: DNA quantification is crucial in life sciences research, and real-time MDA technology can provide template amount information during whole-genome amplification with a very low detection limit, offering a promising tool to quantify entire DNA without sacrificing sample completeness or introducing redundant steps.
Article
Chemistry, Analytical
Liang Gong, Siyue Yao, Yidong He, Chengliang Liu
Summary: A new qPCR data analysis method called AERKM is proposed to achieve more accurate results by introducing amplification efficiency (AE) for error correction. It has been verified to have higher precision and stability in 5-point 10-fold gradient qPCR tests of 63 genes.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Dongfang Zhang, Weikai Ding, Wei Liu, Liuying Li, Gongming Zhu, Junguo Ma
Summary: Chlorpyrifos (CPF) and glyphosate (GLY) are widely used organophosphate insecticide and herbicide, respectively. This study found that exposure to CPF and GLY, alone or in combination, had toxic effects on the brain of carp, including inhibition of acetylcholinesterase and Na+/K+-ATPase activities, alterations in monoamine oxidase levels, decreased antioxidant enzyme activities, and accumulation of malondialdehyde. RNA-seq analysis revealed that exposure to CPF and GLY induced transcriptomic changes in the fish brain, with the combined exposure showing a greater impact than single exposure. These findings provide new insights into the molecular mechanisms of CPF and GLY in fish brains.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Fisheries
Tong Xu, Qiaohan Liu, Dan Chen, Yanyan Liu
Summary: This study reveals that Atrazine exposure induces necroptosis and inflammation in the gills of common carp through the P450/ROS pathway. The findings provide valuable insights into the mechanism of Atrazine-induced injury in common carp.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Jianqing Chen, Bing Shao, Jinliang Wang, Zhiqiang Shen, Honggui Liu, Shu Li
Summary: Chlorpyrifos exposure in common carp gills activated the MAPK/NF-kappa B/TNF-alpha pathway, leading to inflammatory injury and necroptosis with concentration dependency. This study provides valuable data for aquaculture and toxicological research on the toxic effects of CPF.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Zhiwei Chen, Nigel G. Halford, Chenghong Liu
Summary: Real-time quantitative PCR is a technique for measuring the content of a specific nucleic acid sequence in a sample, and it can be divided into absolute and relative quantitative methods. Relative quantification is mainly used for gene expression in functional genomics and transcriptome studies. However, to accurately use this technology, key points such as designing specific primers, selecting appropriate reference genes, and performing scientific calculations and statistics are crucial. This article proposes a workflow for relative quantitative PCR and introduces relevant points to help beginners better understand and utilize this technology.
Article
Fisheries
Zahra Ziafati Kafi, Hamideh Najafi, Mojtaba Alishahi, Hooman Rahmati-Holasoo, Aidin Mouloki, Arash Ghalyanchilangeroudi
Summary: This study confirmed the presence of carp edema virus (CEV) in Iranian freshwater aquaculture. The results also warned of the potential spread of the virus throughout the continent and emphasized the need for restrictions on fish trade.
Article
Chemistry, Applied
Tingting Li, Jishi Wang, Zhiying Wang, Lu Qiao, Rui Liu, Shanshan Li, Ailiang Chen
Summary: Mitochondrial genes are commonly used for PCR-based meat adulteration authentication, but variations in gene copies can lead to quantification errors. This study developed a quantitative method using single-copy nuclear genes to accurately identify mutton adulteration, achieving high recovery rates for simulated mutton-pork, mutton-chicken, and mutton-duck samples.
Article
Multidisciplinary Sciences
Subeen Hong, Seung Mi Lee, Sohee Oh, SoYeon Kim, Young Mi Jung, Sun Min Kim, Chan-Wook Park, Jong Kwan Jun, Byoung Jae Kim, Joong Shin Park
Summary: This study evaluated the performance of a PNA probe-based real-time PCR assay for detecting common aneuploidies. The results showed that the assay had a sensitivity and specificity of 100%. Compared to conventional karyotyping, the PNA probe-based real-time PCR assay was rapid and easy to use.
SCIENTIFIC REPORTS
(2022)
Article
Immunology
Di Wang, Xinya Jiao, Haijiang Jia, Shumei Cheng, Xi Jin, Youhua Wang, Yunhua Gao, Xiaofeng Su
Summary: This study developed a droplet digital PCR (ddPCR) assay for accurate and sensitive detection and quantification of Verticillium dahliae and V. longisporum, showing that ddPCR is more sensitive and accurate than qPCR for quantifying trace amounts of these pathogens in field samples.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Food Science & Technology
Chang-Hun Choi, Eiseul Kim, Seung-Min Yang, Da-Som Kim, Seung-Man Suh, Ga-Young Lee, Hae-Yeong Kim
Summary: This study evaluated the applicability of a ddPCR assay for detecting lactic acid bacteria and compared its performance characteristics to those of a qPCR. The results showed that ddPCR is more sensitive than qPCR, but it has limitations in quantifying high bacterial concentrations.
Article
Reproductive Biology
M. Socha, J. Szczygiel, E. Brzuska, M. Sokolowska-Mikolajczyk, B. Stonawski, M. Grzesiak
Summary: The study found that the herbicide Roundup had negative effects on the embryonic development and survival rate of common carp, leading to reduced hatching rate and increased number of deformed larvae. Significant differences were observed in the transcript of the gene foxr1 in Roundup-exposed groups, while no significant changes were found in hsp70 transcripts.
Article
Environmental Sciences
Mahdi Banaee, Antoni Sureda, Caterina Faggio
Summary: The study showed that Protexin supplementation has a protective effect against chlorpyrifos-induced oxidative stress and immunotoxicity in Cyprinus carpio.
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
(2022)
