Article
Environmental Sciences
Kai Liu, Jiping Li, Yuhao Zhou, Wei Li, Hu Cheng, Jiangang Han
Summary: The combined ecotoxicity of erythromycin (ERY) and roxithromycin (ROX) on microalgae and their removal efficiency during exposure were investigated in this study. The results showed that the mixture of ERY+ROX had an antagonistic effect on Chlorella pyrenoidosa. In addition, the combined treatment had higher antioxidant stress and removal efficiency than individual treatments, especially at low concentrations.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Environmental Sciences
Xueqing Zhong, Xiangxiang Zhang, Tianyi Zhou, Guangping Lv, Quanyu Zhao
Summary: The accumulation of antibiotics in wastewater poses a threat to human health, but microalgae have shown promise in removing antibiotics. This study evaluated the removal kinetics of Tigecycline (TGC) by Chlorella pyrenoidosa under different conditions and identified the main mechanisms for its removal. The results showed that microalgae can effectively remove TGC, especially at higher initial dry cell density and lower TGC per g dry cell weight ratio.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Agricultural Engineering
Yanan Bai, Yuqin Li, Yufang Tang, Rong Zhou, Yiwen Fan
Summary: The study found that using Chlorella pyrenoidosa can efficiently remove pollutants such as TN, TP, NH3-N, COD, and BOD from Rhizopus oryzae fermentation wastewater, and produce high-quality protein fodder. The protein yield was 6.04 times higher than batch manipulation. The levels of pathogenic bacteria and heavy metals in the wastewater met the fodder standards. The method of using Chlorella pyrenoidosa for wastewater treatment can be applied to other microalgal systems.
BIORESOURCE TECHNOLOGY
(2022)
Article
Engineering, Chemical
Edoardo Dell'Armi, Marco Zeppilli, Bruna Matturro, Simona Rossetti, Marco Petrangeli Papini, Mauro Majone
Summary: The study introduces a sequential bioelectrochemical process composed of two membrane-less microbial electrolysis cells operated with synthetic groundwater, leading to a temporary reduction in PCE removal efficiency due to the establishment of sulphate and nitrate reduction processes.
Article
Agronomy
Jun Tang, Jun Yang, Sirui Zhao, Xiaoyu Wang, Zhengxin Xie
Summary: The study investigates the ecotoxicity of tetracycline (TC) on the freshwater alga Chlorella pyrenoidosa and its removal efficiency. The results show that TC has inhibitory effects on microalgal growth and photosynthetic activities but these effects are reversible. C. pyrenoidosa exhibits high removal efficiency for TC through biodegradation and abiotic processes.
Article
Environmental Sciences
Jiping Li, Kai Liu, Wei Li, Meng Zhang, Pingping Li, Jiangang Han
Summary: Microalgae-based biotechnology for antibiotic removal is a promising and environmentally friendly method. This study investigated the mechanism of erythromycin removal by Chlorella pyrenoidosa and its impact on microalgae's ecotoxic response. Biodegradation was found to be the main removal mechanism, while high concentrations of erythromycin inhibited microalgae growth and led to the production of more toxic degradation products.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Biotechnology & Applied Microbiology
Yuming Zhong, Danni Lin, Sufen Li, Qin Wang, Hui Liu, Lukai Ma, Huifan Liu
Summary: We studied the optimal co-culture ratio of Chlorella pyrenoidosa and Yarrowia lipolytica, finding that a 3:1 ratio led to the highest biological nitrogen removal rate and increased removal of chemical oxygen demand, total nitrogen (TN), and ammoniacal nitrogen (NH3-N). TN and NH3-N content decreased in the co-incubated system within 2-6 days compared to the control. mRNA/microRNA (miRNA) expression analysis revealed thousands of differentially expressed genes (DEGs) after 3 and 5 days of co-culture, with 65 DEGs associated with Y. lipolytica nitrogen, amino acid, photosynthetic, and carbon metabolism after 3 days. We also identified differentially expressed miRNAs, two of which negatively correlated with their target mRNA expressions and potentially regulated amino acid metabolism and nitrogen and carbon transport in C. pyrenoidosa.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Engineering, Environmental
Marco Zeppilli, Bruna Matturro, Edoardo Dell 'Armi, Lorenzo Cristiani, Marco Petrangeli Papini, Simona Rossetti, Mauro Majone
Summary: A bioelectrochemical process combining two membrane-less reactors equipped with graphite granules counter electrode was developed for perchloroethylene removal. The reductive reactor completely reduced PCE into VC and Eth, which were efficiently removed by the oxidative reactor. Different redox niches in each compartment allowed the co-existence of both reducing and oxidative reactions, enhancing the overall efficiency of PCE removal.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2021)
Article
Engineering, Environmental
Edoardo Dell'Armi, Marco Zeppilli, Maria Letizia Di Franca, Bruna Matturro, Viktoria Feigl, Monika Molnar, Zsofia Berkl, Imre Nemeth, Hafsa Yaqoubi, Simona Rossetti, Marco Petrangeli Papini, Mauro Majone
Summary: In this study, a sequential reductive/oxidative bioelectrochemical process was used to remove chlorinated aliphatic hydrocarbons (CAHs) from contaminated groundwater. The sequential system consisted of two membrane-less microbial electrolysis cells (MECs) connected in series. The results showed that the sequential process effectively mineralized CAHs and reduced their ecotoxicity, as evaluated by biomarkers and ecotoxicity tests.
JOURNAL OF WATER PROCESS ENGINEERING
(2022)
Article
Environmental Sciences
Zhengxin Xie, Xiaoyu Wang, Ying Gan, Haomiao Cheng, Shisuo Fan, Xuede Li, Jun Tang
Summary: This study assessed the ecotoxicological effects of the antidepressant fluoxetine (FLX) on the freshwater microalgae Chlorella pyrenoidosa, and investigated the removal efficiency and degradation pathway of FLX by the microalgae. The results showed that FLX inhibited the growth and photosynthesis of C. pyrenoidosa, but the microalgae exhibited adaptability to FLX and were able to remove it efficiently.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Agricultural Engineering
Xiao-Bo Tan, Lu Wang, Xi-Ping Wan, Xiao-Ni Zhou, Li-Bin Yang, Wen-Wen Zhang, Xian-Chao Zhao
Summary: Pure toilet wastewater is the best medium for cultivating oleaginous microalgae, while discharge of kitchen or laundry wastewater brings risks for biomass and lipids production. It is recommended to separate kitchen or laundry wastewater from toilet wastewater before entering into septic tank.
BIORESOURCE TECHNOLOGY
(2021)
Article
Microbiology
Maria L. Di Franca, Bruna Matturro, Simona Crognale, Marco Zeppilli, Edoardo Dell'Armi, Mauro Majone, Marco Petrangeli Papini, Simona Rossetti
Summary: This study describes the microbiome composition of a novel bioelectrochemical system (BES) for removing perchloroethylene using sequential reductive/oxidative dechlorination. The feeding composition affects the microbiome and the products of the dechlorination reaction.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Plant Sciences
Ruohan Huang, Wan Liu, Jinghua Su, Shihao Li, Liqing Wang, Erik Jeppesen, Wei Zhang
Summary: In recent years, antibiotics pollution has had a serious impact on the aquatic environment, and the degradation of antibiotics by microalgae has gained attention. However, further research is needed to understand the potential toxicity of antibiotics to microalgae species and the impact of microalgal diversity on antibiotic removal. This study investigated the removal efficiency and tolerance of five freshwater microalgae and their consortia to sulfamethoxazole (SMX). The results showed that one microalgae species, Chlorella pyrenoidosa, had a higher removal efficiency compared to the other four species. The study also found that microalgae consortia showed lower removal efficiency than C. pyrenoidosa alone, indicating that the number of microalgae species did not necessarily increase antibiotic removal.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Environmental Sciences
Xianglin Liu, Yanyao Li, Xiaowei Zheng, Liangliang Zhang, Haoxuan Lyu, Honghui Huang, Zhengqiu Fan
Summary: GenX, a substitute for perfluorooctanoic acid, has been widely detected in surface water and has potential ecological hazards. Research shows that acute exposure to GenX inhibits the growth of Chlorella pyrenoidosa and damages its photosynthetic system, but the up-regulation of related genes provides an antioxidant mechanism. This study provides new insights into the antioxidant capacity to cope with the oxidative stress caused by acute GenX exposure.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Engineering, Environmental
Zhongqi He, Xiumin Fan, Luyao Qu, Xu Zhou, Wenbiao Jin, Mohammad Rafe Hatshan, Xuan Li, Huan Liu, Guangming Jiang, Qilin Wang
Summary: The study found that Scenedesmus obliquus and Chlorella pyrenoidosa can effectively treat swine wastewater, and Scenedesmus obliquus has higher biomass and chlorophyll-a content, making it more suitable for treating swine wastewater and accumulating biomass.
PROCESS SAFETY AND ENVIRONMENTAL PROTECTION
(2023)
Article
Environmental Sciences
Wei Huang, Guodong Cao, Chengliang Deng, Yanyan Chen, Tao Wang, Da Chen, Zongwei Cai
Summary: This study investigated the effects of TCS exposure on kidney function in mice. The results showed that TCS caused renal injury, oxidative stress, inflammation, and fibrosis in a dose-dependent manner. Metabolite analysis revealed lipid accumulation and defective fatty acid metabolism in TCS-exposed mouse kidney, which may contribute to renal function impairment.
JOURNAL OF ENVIRONMENTAL SCIENCES
(2023)
Article
Multidisciplinary Sciences
Chen Xiao, Kaien Li, Jingmin Hua, Zhao He, Feng Zhang, Qiongfang Li, Hailei Zhang, Lei Yang, Shuying Pan, Zongwei Cai, Zhiling Yu, Kam-Bo Wong, Yiji Xia
Summary: Arabidopsis DXO1 is an essential component in the methylation of the guanosine cap to form the m(7)G cap. It activates RNA guanosine-7 methyltransferase (AtRNMT1) to catalyze this conversion. This study reveals an important mechanism in m(7)G capping in plants and the role of DXO1 in this process.
NATURE COMMUNICATIONS
(2023)
Article
Nanoscience & Nanotechnology
Chao Zhong, Guorong Li, Wenchang Tian, Dan Ouyang, Yin Ji, Zongwei Cai, Zian Lin
Summary: Enzyme immobilization is crucial for its application in various fields. Covalent organic frameworks (COFs) are ideal for enzyme immobilization due to their controlled porous structures. However, constructing COFs-based enzyme immobilization systems with high activity using existing methods is still challenging.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Analytical
Peiru Luo, Mengke Bai, Qingyun He, Zifang Peng, Lingyun Wang, Chuan Dong, Zenghua Qi, Wenfen Zhang, Yanhao Zhang, Zongwei Cai
Summary: The widespread use of plastic products has led to a significant release of microplastics (MPs) into the environment. However, the study of MPs in atmospheric fine particulate matter (PM2.5) is limited due to the lack of appropriate analytical methods. This study developed a novel strategy using pyrolysis-gas chromatography-tandem mass spectrometry (Pyr-GC-MS/MS) to accurately quantify polyethylene MPs (PE-MPs) in PM2.5 without sample pretreatment. The results showed relatively serious pollution of PE-MPs in Taiyuan and suggested the widespread occurrence and potential human exposure risks of PM2.5-bound PE-MPs.
ANALYTICAL CHEMISTRY
(2023)
Article
Engineering, Environmental
Hongli Tan, Liu Yang, Xiaolin Liang, Diedie Huang, Xinhang Qiao, Qingyuan Dai, Da Chen, Zongwei Cai
Summary: With the tightening restrictions on phthalate esters (PAEs), nonphthalate plasticizers (NPPs) are increasingly used. However, there is limited knowledge on the environmental occurrences, fate, and human exposure risks of many emerging NPPs. This study investigated the presence of NPPs and major PAEs in house dust, finding widespread occurrences of NPPs, such as ATBC, TCTM, TOTM, GMO, MO, and DiBA. Although current exposure may not pose significant health risks, potential hazards cannot be overlooked due to the lack of toxic threshold data and possible cocktail effects.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Chemistry, Analytical
Jing Chen, Peisi Xie, Qingyuan Dai, Pengfei Wu, Yu He, Zian Lin, Zongwei Cai
Summary: In this study, MALDI-2 and TIMS imaging were used to analyze lipids and metabolites in H295R adrenocortical MCTS. MALDI-2 detected more lipids and metabolites in MCTS compared to traditional MALDI, while TIMS successfully separated isomeric and isobaric ions in different regions of MCTS. MS/MS imaging experiments further confirmed the presence of these isomeric and isobaric ions. These findings demonstrate the potential of MALDI-2 and TIMS imaging as valuable tools for lipid and metabolite analysis in MCTS for various biological and medical research fields.
Article
Chemistry, Analytical
Xiaojian Shao, Hailei Zhang, Zhou Zhu, Fenfen Ji, Zhao He, Zhu Yang, Yiji Xia, Zongwei Cai
Summary: The recent discovery of noncanonical RNA caps, such as NAD(+) and dpCoA, has expanded our understanding of RNA caps. To identify and characterize dpCoA-RNAs, a method called dpCoA tagSeq was developed. This method efficiently tags and captures dpCoA-capped RNAs, allowing for further functional investigations.
ANALYTICAL CHEMISTRY
(2023)
Article
Engineering, Environmental
Jing Chen, Peisi Xie, Pengfei Wu, Zian Lin, Yu He, Zongwei Cai
Summary: This study used metabolomics and lipidomics combined with mass spectrometry imaging to reveal the mechanisms of enhanced growth of MCF-7 breast cancer cell spheroids exposed to triclosan (TCS). The results showed that TCS and TCS sulfate penetrated the entire area of the cell spheroids initially, but localized in the inner area later. Metabolomics data indicated that TCS exposure induced alterations in energy metabolism and the biosynthesis of glycerophospholipids and glycerolipids. Mass spectrometry imaging revealed enhanced energy supply in the peripheral area and increased energy storage in the inner area as potential contributors to the enhanced growth of MCF-7 breast cancer cell spheroids exposed to TCS.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Engineering, Environmental
Xuemei Wang, Chin Wai Leung, Zongwei Cai, Di Hu
Summary: Organophosphate flame retardants (OPFRs) were found in PM2.5 in Hong Kong, with chlorinated OPFRs being the dominant component. Plastic processing and waste disposal were identified as the main emission sources. Health risk assessment revealed a low-risk level for PM2.5-bound OPFRs, but relatively high noncarcinogenic and carcinogenic risks were associated with chlorinated OPFRs emitted from plastic processing and waste disposal.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Engineering, Environmental
Guodong Cao, Wei Wang, Jing Zhang, Pengfei Wu, Han Qiao, Huankai Li, Gefei Huang, Zhu Yang, Zongwei Cai
Summary: para-Phenylenediamine quinones (PPD-Qs) are newly discovered transformation products derived from para-phenylenediamine (PPD) antioxidants. Their occurrence and fate in wastewater treatment plants (WWTPs) need further investigation to improve pollutant removal efficiency.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Chemistry, Analytical
Gefei Huang, Feng Zhang, Dongying Xie, Yiming Ma, Pengxi Wang, Guodong Cao, Leijian Chen, Siyi Lin, Zhongying Zhao, Zongwei Cai
Summary: In this study, a high-throughput analytical platform combining ultra-performance liquid chromatography (UPLC) and complementary mass spectrometry (MS) was developed to identify and quantify RNA modifications. Sixty-four types of RNA modifications were successfully monitored within a 16-minute single run of UPLC-MS. The method can also aid in the discovery of RNA-modifying enzymes in genetic studies.
Article
Environmental Sciences
Xiaoou Wei, Jiang Yu, Yong Tian, Yujie Ben, Zongwei Cai, Chunmiao Zheng
Summary: Accurately predicting influent wastewater quality is crucial for the efficient operation and maintenance of wastewater treatment plants. This study evaluated three machine learning models and successfully applied them to three full-scale WWTPs in Shenzhen, China. The study provides a model framework for forecasting wastewater loads and sets the groundwork for smart management.
Article
Chemistry, Analytical
Li Xiang, Yi Ru, Jingchun Shi, Hongzhi Zhao, Yu Huang, Zongwei Cai, Li Wang
Summary: N-Acyl glycines (NAGlys) are important metabolites in the human body's detoxification system, and can be used in the diagnosis of metabolic diseases. A simple and sensitive method of NAGlys detection by LC-MS in plasma and urine samples is described, using 3-nitrophenylhydrazine as a derivatization reagent. This approach allows for a broader measurement of NAGlys, especially in the absence of authentic standards. The identified NAGlys have the potential to be diagnostic biomarkers for type II diabetes and diabetic complications.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Peige Qin, Shiping Zhu, Mengyao Mu, Yanmei Gao, Zongwei Cai, Minghua Lu
Summary: A new hierarchical mixed-dimensional metal-organic framework (MOF@MOF) hybrid material was prepared for the removal and enrichment of environmental pollutants. The material exhibited excellent adsorption performance for bisphenols (BPs) due to its extensive mesoporous structures and large surface area.
CHINESE CHEMICAL LETTERS
(2023)
Article
Chemistry, Analytical
Jiajing Chen, Huan Huang, Dan Ouyang, Jiali Lin, Zhuling Chen, Zongwei Cai, Zian Lin
Summary: Analysis of cis-diol compounds is crucial due to their important roles in various fields. Researchers have developed a matrix-assisted laser desorption/ionisation mass spectrometry (MALDI-MS) method to effectively analyse cis-diol compounds. This method utilizes a unique reactive matrix (BMQI) for in situ derivatization of cis-diol compounds, resulting in improved speed, reproducibility, and signal intensity, as well as reduced background interference. Additionally, the method has been successfully applied to the rapid screening and identification of sugar alcohols in different sugar-free foods.
