期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 54, 期 8, 页码 2538-2542出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201411040
关键词
charge-based detection; nano-oscillators; phosphorylation; plasmonic imaging; real-time imaging
资金
- National Natural Science Foundation of China (NSFC) [21327008, 21405080, 21327902]
- Natural Science Foundation of Jiangsu Province [BK20140592]
- W.N. Keck Fundation
Phosphorylation is a post-translational modification that is involved in many basic cellular processes and diseases, but is difficult to detect in real time with existing technologies. A label-free detection of phosphorylation is reported in real time with self-assembled nano-oscillators. Each nano-oscillator consists of a gold nanoparticle tethered to a gold surface with a molecular linker. When the nanoparticle is charged, the nano-oscillator can be driven into oscillation with an electric field and detected with a plasmonic imaging approach. The nano-oscillators measure charge change associated with phosphorylation of peptides attached onto a single nanoparticle, allowing us to study the dynamic process of phosphorylation in real time without antibodies down to a few molecules, from which Michaelis and catalytic rate constants are determined.
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