Article
Chemistry, Analytical
Yanyan Ma, Zhanchen Guo, Chuanwen Fan, Jingran Chen, Shuxin Xu, Zhen Liu
Summary: This study developed aptamers that can target ABCG2 and reverse multidrug resistance, showing great potential in cancer diagnosis and treatment. The aptamers can specifically bind to human colorectal cancer stem cells and effectively reverse drug resistance in liver cancer cells.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Hedvig Tordai, Erzsebet Suhajda, Ian Sillitoe, Sreenath Nair, Mihaly Varadi, Tamas Hegedus
Summary: In recent years, the number of unique transmembrane protein structures has doubled, thanks to the advancement of cryo-electron microscopy and AlphaFold2. However, collecting structures for specific protein families remains challenging. This study demonstrates the applicability and usability of automatic collection and presentation of protein structures through the ABC protein superfamily.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Daniel W. Valencia, Ady B. Melendez, Isaac A. Melendrez, Erik T. Yukl
Summary: ATP binding cassette (ABC) transporters are the primary means by which bacteria acquire trace elements from the environment. They rely on solute binding proteins (SBPs) to bind the relevant substrate and deliver it to the integral membrane permease for ATP-powered import into the cytoplasm. A newly discovered family of zinc SBPs, represented by ZrgA proteins, has been found in Gram-negative bacteria, including human pathogens.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Virapat Kieuvongngam, Jue Chen
Summary: This study explores the structure and function of the peptidase-containing ABC transporter 1 (PCAT1) and reveals two different conformational distributions depending on the presence or absence of ATP and Mg2+. The ATP-bound, NBD-dimerized conformation is found to be the lowest energy state, while the rate-limiting step in the PCAT1 transport cycle is the formation of the NBD dimer.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Amira Sayed Hanafy, Pia Steinlein, Julika Pitsch, Mariella Hurtado Silva, Natascha Vana, Albert J. Becker, Mark Evan Graham, Susanne Schoch, Alf Lamprecht, Dirk Dietrich
Summary: The authors report a method combining micro-perfusion and multiphoton microscopy to study the transport and permeability of the blood-brain barrier (BBB). This method allows monitoring of BBB transport at the cellular level, visualization of permeation of cells and molecules in real-time and provides details of the neurovascular unit. It is a valuable tool for translational research and analysis of therapeutic strategies.
NATURE COMMUNICATIONS
(2023)
Review
Biochemistry & Molecular Biology
Narakorn Khunweeraphong, Karl Kuchler
Summary: Multidrug resistance can be a major issue in cancer treatment and microbial infections, with dysregulated overexpression of ATP-binding cassette transporter members being linked to this phenomenon. High-resolution atomic structures from X-ray crystallography or cryo-EM are helping to improve understanding of the mechanisms and dynamics driving these transporters, offering new perspectives for therapeutic interventions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Microbiology
Ali Raza, Andrew R. Williams, Muhammad Mustafa Abeer
Summary: ABC transporters are ATP-dependent transmembrane proteins responsible for active transport of various molecules across cell membranes. They play a crucial role in the resistance of parasitic nematodes to anthelmintic drugs and could be targeted for nematode control strategies. This review discusses the role of ABC transporters, their regulation, and their association with anthelmintic resistance, as well as the possibility of targeting them with inhibitors or nutraceuticals for controlling parasitic infections.
Article
Biochemistry & Molecular Biology
Jianyi Li, Yuntong Lv, Kunpeng Yan, Fengting Yang, Xuewei Chen, Xiwu Gao, Shuyuan Wen, Hongfei Xu, Yiou Pan, Qingli Shang
Summary: The study revealed that ATP-binding cassette transporters (ABCs) play important roles in cyantraniliprole resistance. Inhibition of ABCs increased the toxicity of cyantraniliprole, while overexpression of ABCs in fruit flies enhanced their tolerance to cyantraniliprole. Suppression of specific ABC transporters increased the sensitivity of cyantraniliprole-resistant strains. These findings provide valuable insights for resistance management strategies.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Chang Xu, Xiyun Jin, Songren Wei, Pingping Wang, Meng Luo, Zhaochun Xu, Wenyi Yang, Yideng Cai, Lixing Xiao, Xiaoyu Lin, Hongxin Liu, Rui Cheng, Fenglan Pang, Rui Chen, Xi Su, Ying Hu, Guohua Wang, Qinghua Jiang
Summary: This study presents DeepST, an accurate and universal deep learning framework, which outperforms existing methods in identifying spatial domains. It can dissect spatial domains in cancer tissue at a finer scale and integrate ST data generated from multiple batches or different technologies effectively. These results demonstrate that DeepST is a powerful tool for gaining novel insights from ST studies.
NUCLEIC ACIDS RESEARCH
(2022)
Review
Biochemistry & Molecular Biology
Ian D. Kerr, Ella Hutchison, Louise Gerard, Shereen M. Aleidi, Ingrid C. Gelissen
Summary: This review provides an update on the function of ABC transporter subfamily members in lipid homeostasis and drug transport, focusing on ABCG1, ABCG2, ABCG4, ABCG5, and ABCG8. Progress in understanding their role in health and disease, recognized transporter substrates, post-translational regulation, microRNA involvement, and structural elements important for transport activity are discussed. Additionally, the impact of lipids such as cholesterol on ABCG2 transport function and a potential model of cholesterol binding sites for ABCG2 are described.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2021)
Article
Biochemistry & Molecular Biology
Dianjie Xie, Cong Zhu, Lei Zhang, Yueqiu Liu, Yunxia Cheng, Xingfu Jiang
Summary: The oriental armyworm Mythimna separata is a polyphagous corn pest in Asia. Transgenic Bt corn can effectively control this insect pest. This study identified 43 ABC transporter genes in the M. separata genome, and found that MsABCC2 may play a more important role in Cry1Ac toxicity and is a potential Cry1Ac receptor in M. separata. These findings provide valuable information for understanding the role of ABC transporter genes in M. separata and the long-term application of Bt insecticidal protein.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biotechnology & Applied Microbiology
Lilia De la Torre, Jose G. Vergara Meza, Sindy Cabarca, Andre G. Costa-Martins, Andrea Balan
Summary: This study compared four ABC transporter systems in Mycobacterium tuberculosis dedicated to carbohydrate uptake and transport. The components of these transporters were phylogenetically separated into four groups based on structural differences, affecting functional activity or interactions with domain partners. The presence of specific transporters in pathogenic species highlights their relevance in virulence and pathogenesis, and differences in components can be explored for drug development targeting the bacillus.
Review
Environmental Sciences
Tapan Behl, Aayush Sehgal, Madhuri Grover, Sukhbir Singh, Neelam Sharma, Saurabh Bhatia, Ahmed Al-Harrasi, Lotfi Aleya, Simona Bungau
Summary: Metabolic disorders such as diabetes, obesity, and cardiovascular complications are increasing worldwide. The dysregulation of lipid and glucose transporters plays a critical role in the onset of diabetes mellitus which requires the search for new therapeutic targets. Studies suggest the involvement of ATP-binding cassette transporters in different diseases, highlighting their potential as treatment targets for insulin resistance and dysregulated glucose metabolism in diabetes.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Microbiology
Anastasios Samaras, George S. Karaoglanidis, Georgios Tzelepis
Summary: The biocontrol agent Bacillus subtilis MBI 600 has been found to inhibit mycelial growth of the plant pathogen B. cinerea in vitro and reduce disease occurrence in cucumber plants. Treatment with B. subtilis induced genes related to plant immunity, indicating its potential role in disease control.
MICROBIOLOGICAL RESEARCH
(2021)
Review
Biochemistry & Molecular Biology
Michele Galluccio, Lara Console, Lorena Pochini, Mariafrancesca Scalise, Nicola Giangregorio, Cesare Indiveri
Summary: Membrane transport systems play a crucial role in maintaining cell homeostasis and drug interactions. E. coli is a preferred host for expressing human membrane proteins, but their hydrophobicity and structural differences pose challenges. This review summarizes various strategies for expressing human transport systems in bacteria.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)