期刊
CHEMISTRY-A EUROPEAN JOURNAL
卷 24, 期 65, 页码 17239-17254出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.201803178
关键词
antitumor agents; boron neutron capture therapy (BNCT); DNA recognition; intercalations; metallacarboranes; redox chemistry
资金
- Spanish Ministerio de Economia y Competitividad [CTQ2016-75150-R, CTQ2015-71955-REDT, CTQ2014-53334-C2-1-R]
- Generalitat de Catalunya [2017 SGR 1720]
- Comunidad Autonoma de Madrid NANOAVANSENS [S2013/MIT-3029]
- Ministry of Education, Culture, Sports, Science and Technology, Japan [17H02202]
- FCT [UID/Multi/04349/2013]
- Grants-in-Aid for Scientific Research [17H02202] Funding Source: KAKEN
After uptake by U87 MG and A375 cancer cells, cobaltabisdicarbollide [COSAN](-) distributes between membrane and nucleus and presents no relevant cytotoxicity against both cell lines even for long incubation times. The cytotoxicity of Na[COSAN] was also tested towards one normal cell line, the V79 fibroblasts, in order to ascertain the noncytotoxic profile of the compound. As the cell's nucleus contains DNA, the interaction between [COSAN](-) and double-stranded calf thymus DNA (CT-dsDNA) has been investigated. There is a strong interaction between both molecules forming a nanohybrid CT-dsDNA-[COSAN] biomaterial, which was fully characterized. Moreover, Na[COSAN] shows characteristic redox peaks ascribed to the oxidation/reduction of Co3+/2+ at a formal potential of -1.444 V and it can be accumulated at a surface-immobilized DNA layer of glassy carbon electrodes. The equilibrium surface-binding constants (K-ox/K-red), which confirm that [COSAN](-) interacts with DNA by an intercalative or electrostatic mode, depending on the ionic strength of the solution, were estimated. In addition, high binding affinity of Na[COSAN] to proteins was observed by B-11{H-1} NMR and confirmed in vivo. Finally, biodistribution studies of [COSAN](-) in normal mice were run. After administration, Na[COSAN] was distributed into many organs but mainly accumulated in the reticuloendothelial system (RES), including liver and spleen. After 1 h, the formation of aggregates by plasma protein interaction plays a role in the biodistribution profile; the aggregates accumulate mostly in the lungs. Na[COSAN], which displays low toxicity and high uptake by relevant cancer cells accumulating boron within the nucleus, could act as a suitable compound for further developments as boron neutron capture therapy (BNCT) agents.
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