期刊
CHEMISTRY-A EUROPEAN JOURNAL
卷 20, 期 48, 页码 15768-15774出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.201404499
关键词
anions; ATP; enzymes; fluorescent probes; quinolines
资金
- Ramsay Memorial Fellowship Trust
- Durham University
Two tripodal fluorescent probes Zn.L-1,L-2 have been synthesised, and their anion-binding capabilities were examined by using fluorescence spectroscopy. Probe Zn.L-1 allows the selective and ratiometric detection of adenosine triphosphate (ATP) at physiological pH, even in the presence of several competing anions, such as ADP, phosphate and bicarbonate. The probe was applied to the real-time monitoring of the apyrase-catalysed hydrolysis of ATP, in a medium that mimics an extracellular fluid.
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