期刊
CHEMISTRY-A EUROPEAN JOURNAL
卷 20, 期 37, 页码 11760-11767出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.201402999
关键词
anions; chlorine; fluorescence; imaging agents; lysosomes
资金
- 973 Program [2013CB933800]
- National Natural Science Foundation of China [21227005, 21390411, 91313302, 21035003, 21205073]
- Program for Chang jiang Scholars and Innovative Research Team in University
Fluorescence ratio imaging is currently being used to quantitatively detect biologically active molecules in bio-systems; however, two excitations of most existing fluorescent ratiometric probes account for cumbersome operating conditions for imaging. Thus, a fluorescent ratiometric probe, 6-methoxyquinolinium-dansyl (MQ-DS), for Cl- with single excitation/dual maximum emission has been developed. MQ-DS can preferably localize into lysosomes and display excellent photostability. Upon excitation at a single wavelength, it responds precisely and instantaneously to changes in Cl- concentrations, and it can be conveniently utilized to implement real-time fluorescence ratio imaging to quantitatively track alterations in Cl- levels inside cells treated under various pH conditions, and also in zebrafish with acute wounds. The successful application of the new probe in bioimaging may greatly facilitate a complete understanding of the physiological functions of Cl-.
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