期刊
CHEMISTRY-A EUROPEAN JOURNAL
卷 18, 期 13, 页码 4080-4087出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.201103270
关键词
aldehydes; DNA; nucleotides; peptides; reductive aminations
资金
- Academy of Sciences of the Czech Republic [Z40550506]
- Czech Science Foundation [203/09/0317]
- Gilead Sciences, Inc. (Foster City, CA, USA)
5-(5-Formylthienyl)-, 5-(4-formylphenyl)- and 5-(2-fluoro-5-formylphenyl)cytosine 2'-deoxyribonucleoside mono- (dCRMP) and triphosphates (dCRTP) were prepared by aqueous SuzukiMiyaura cross-coupling of 5-iodocytosine nucleotides with the corresponding formylarylboronic acids. The dCRTPs were excellent substrates for DNA polymerases and were incorporated into DNA by primer extension or PCR. Reductive aminations of the model dCRMPs with lysine or lysine-containing tripeptide were studied and optimized. In aqueous phosphate buffer (pH 6.7) the yields of the reductive aminations with tripeptide III were up to 25?%. Bioconjugation of an aldehyde-containing DNA with a lysine-containing tripeptide was achieved through reductive amination in yields of up to 90?% in aqueous phosphate buffer.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据