Article
Biophysics
Narges Ashrafi, Behzad Shareghi, Sadegh Farhadian, Mansoore Hosseini-Koupaei
Summary: This study investigated the effect of polyamine on the compound and function of lysozyme using UV-Visible and fluorescence spectroscopy as well as simulation method. The results showed that increasing polyamine concentration reduced the intrinsic quenching fluorescence of lysozyme and altered its secondary and tertiary structures. Polyamine also inhibited the enzymatic activity of lysozyme.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Article
Biochemistry & Molecular Biology
Riddhi P. Dave, Man Singh
Summary: This study designed thermodynamically, kinetically, and tentropically stable homogeneous solutions of bovine serum albumin (BSA 66), hemoglobin (Hb 64.5), and double stranded deoxyribonucleic acid (dsDNA 130 kDa) with water and different concentrations of aqueous carboxymethyl cellulose sodium salt (NaCMC). The physicochemical properties of these solutions were studied, and various methods were used to analyze their structural recognition and interaction. The results showed that the interaction mechanism of BSA, Hb, and dsDNA with NaCMC involved salt bridges, electrostatic dipoles, and hydrogen bonds, and these interactions could affect the solubilization of biomolecules. The findings of this study have implications for areas such as biochemical, biophysical, bioengineering, tissue, and genetic engineering, and can be extended to pharmaceutical and cosmetic processes.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Gayane Ananyan, Nelli H. Karapetyan, Yeva B. Dalyan
Summary: The binding characteristics of water-soluble porphyrins and their Cu and Co derivatives with synthetic polynucleotides were studied, showing different binding modes at different concentrations. The calculated binding constants and exclusion parameters for each complex aligned well with proposed models.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2022)
Article
Chemistry, Organic
Jonathan Alvarez-Garcia, Victor Rubio-Pisabarro, Luis Garcia-Rio, Maria Magdalena Cid
Summary: Proton transfer equilibria play a key role in various biological and pharmaceutical processes. The behavior of chiral pyrido-cyclophanes in the presence of different acids was studied, revealing double protonation and formation of cascade ion-pairs. Water presence promoted the crystallization of intermediates not corresponding to those in solution, and proton transfer from acid to pyridine was found to occur regardless of acid's pKa. The hydrogen position after proton transfer equilibria was elucidated by diagnostic signals in ECD of cascade complexes.
ORGANIC CHEMISTRY FRONTIERS
(2023)
Article
Chemistry, Multidisciplinary
David Pal, Celine Besnard, Adiran de Aguirre, Amalia I. Poblador-Bahamonde, Gennaro Pescitelli, Jerome Lacour
Summary: Hydrogen bonding plays a significant role in chemical reactions, conformation, and stereochemistry. This study introduces a novel dye, tetranitrofluorenone oximate, which can visually detect hydrogen-bond donating species and quickly determine the strength of hydrogen bonding by monitoring hypsochromic shifts. The molecule also exhibits atropisomeric conformations, and enantiopure bis-thioureas are found to be the most effective hydrogen bond donors for chiral induction, as demonstrated by electronic circular dichroism and time-dependent density functional theory calculations. Furthermore, bis-thioureas are used as chiral reagents in asymmetric 1,3-dipolar cycloadditions, with the electronic circular dichroism sensing of stereoinduction serving as an indirect method to select the most effective hydrogen bond donor for asymmetric synthesis.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Chemistry, Physical
Biyu Sun, Xin Ren, Jing Li, Jianing Chen
Summary: In this paper, we theoretically explore the physical mechanism of the fluorescent origin and chiral nature of Möbius carbon nanobelts. The electron-hole recombination is promoted by the electron-hole aggregation on the twisted moiety, resulting in strong fluorescence. The chiral nature of Möbius carbon nanobelts is revealed by the interaction between the transition electric and the magnetic dipole moments, causing the observed Cotton effects in the ECD spectra. Möbius strip molecules are inherently chiral due to their topology, generating two non-superimposable mirror images.
JOURNAL OF PHYSICAL CHEMISTRY C
(2023)
Article
Biochemistry & Molecular Biology
Vania Gomes, Nuno Mateus, Victor de Freitas, Luis Cruz
Summary: Stimuli-responsive pigments, particularly a new xanthylium-derived pigment, were synthesized and studied for their chemical and photophysical properties in hydroalcoholic solutions at different pH values. The pigment showed interesting pH-dependent emission behavior with potential applications as a pH-responsive fluorescent probe for biological purposes.
PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES
(2021)
Article
Food Science & Technology
Jessica Laika, Giampiero Sacchetti, Annalaura Sabatucci, Junior Bernardo Molina-Hernandez, Antonella Ricci, Romolo Laurita, Silvia Tappi, Alessandro Di Michele, Lilia Neri
Summary: This study investigated the effect of different CAP exposures on the activity of horseradish peroxidase (HRP) and the potential role of sugars in enzyme inactivation. The results showed that the inactivation of HRP in phosphate buffer depended on both treatment time and ozone concentration. Sugars reduced the efficacy of CAP, with a greater effect observed for disaccharides. In addition, sugars preserved the tertiary structure of HRP and prevented protein loss. This study deepened our understanding of the effect of CAP on peroxidase activity and demonstrated the effectiveness of sugars in mitigating enzyme inactivation.
Article
Chemistry, Multidisciplinary
Masahide Tominaga, Tadashi Hyodo, Yuya Hikami, Kentaro Yamaguchi
Summary: A new host molecule with pyridazine moieties was synthesized and its crystal structures were studied to investigate halogen interactions. The experiments showed that crystals obtained from different solvents exhibited various types of halogen interactions.
Article
Biochemistry & Molecular Biology
Nuno M. M. Moura, Jose A. S. Cavaleiro, Maria Graca P. M. S. Neves, Catarina I. V. Ramos
Summary: As tumours are closely related to the telomerase function and oncogene expression, the structure of enzymes and genes involved are being targeted for new anticancer drugs. The use of ligands to stabilize G4 DNA structures, such as the telomeric DNA sequence, is considered a promising strategy for cancer therapies. This study evaluated the efficacy of three tetracationic opp-dibenzoporphyrins in stabilizing G4 structures, with the free-base porphyrin and its zinc(II) complex showing good affinity and selectivity for the telomeric G4 structure.
Article
Chemistry, Physical
Liya Dai, Pengyao Xing, Aiyou Hao
Summary: In this study, the aryl-group achiral molecular tweezers were found to effectively include chiral guest molecules via halogen bonding and other noncovalent interactions. Experimental and computational calculations revealed the formation of stable 1:1 host-guest complexes, demonstrating the transfer of chirality to achiral host molecules at the supramolecular level.
JOURNAL OF PHYSICAL CHEMISTRY C
(2023)
Review
Chemistry, Organic
Gabriele Travagliante, Massimiliano Gaeta, Roberto Purrello, Alessandro D'Urso
Summary: Self-assembly is a powerful strategy for constructing complex supramolecules. Water-soluble porphyrinoids, which can form aggregates in aqueous media via weak non-covalent forces, are ideal paradigms to study self-assembly. Studies have shown that even achiral porphyrin aggregates can exhibit supramolecular chirality. This mini-review discusses strategies for designing and building chiral aggregates in water from achiral porphyrin monomers, with a particular focus on their chiroptical features.
CURRENT ORGANIC CHEMISTRY
(2022)
Article
Spectroscopy
Yinan Xue, Jintao Tian, Weiguo Tian, Kai Zhang, Junji Xuan, Xinghua Zhang
Summary: Spiropyran compounds are important photochromic molecules used as detectors and fluorescence probes in various fields. A spiropyran derivative with a hydroxyl group was used in this study to recognize different types of amines based on their UV-Vis absorption spectra. Chemical reactions between primary amines and SPOH, as well as alkalinity, were identified as vital interaction mechanisms for the recognition process.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Biochemistry & Molecular Biology
Milena Mlakic, Ivona Cipor, Petra Kovacec, Goran Kragol, Ana Ratkovic, Tatjana Kovacevic, Rahela Zadravec, Valentina Milasinovic, Kresimir Molcanov, Ivo Piantanida, Irena Skoric
Summary: A new series of 4H-1,3-benzothiazine dyes were synthesized and characterized. Two synthetic pathways, Buchwald-Hartwig amination and electrochemical synthesis, were used to prepare benzothiazine salts. Electrochemical intramolecular dehydrogenative cyclization was employed to form 4H-1,3-benzothiazines. The binding of these compounds to polynucleotides was examined, and compounds 1 and 2 showed potential as DNA/RNA groove binders.
Article
Chemistry, Applied
Sara M. Tome, Raquel G. Soengas, Rosana Badia-Laino, Artur M. S. Silva
Summary: A novel family of (E,E)-aminoaurone-type compounds with an extended pi-system has been successfully synthesized via copper catalysed aza-Michael addition, ring opening, and cyclization. These compounds exhibit strong solvatochromic absorption and emission properties, making them suitable for sensing applications. In particular, compound 3h shows promising characteristics with a large Stokes shift and the presence of a C-glycosyl moiety. Appropriately substituted pi-extended aminoaurones are potentially useful as biological probes.
Article
Chemistry, Analytical
Marta Koscak, Ivona Krosl, Biserka Zinic, Ivo Piantanida
Summary: In this study, novel peptidoids were synthesized by replacing and extending amino acids in a peptide sequence, and these peptidoids demonstrated similar metal ion binding properties as the parent peptide. Some of the peptidoids showed high affinity to Cu2+ ions and exhibited fluorescent and chiral circular dichroism responses upon Cu2+ ion binding. Additionally, these peptidoids were capable of binding to DNA and RNA, and Cu2+ ions were found to enhance the stability of the peptidoid-DNA/RNA complexes. These findings provide important insights for further investigations on the interaction between peptides and metal ions.
Article
Chemistry, Multidisciplinary
Goutam Kumar Kole, Marta Koscak, Anissa Amar, Dragomira Majhen, Ksenija Bozinovic, Zlatko Brkljaca, Matthias Ferger, Evripidis Michail, Sabine Lorenzen, Alexandra Friedrich, Ivo Krummenacher, Michael Moos, Holger Braunschweig, Abdou Boucekkine, Christoph Lambert, Jean-Francois Halet, Ivo Piantanida, Klaus Mueller-Buschbaum, Todd B. Marder
Summary: A series of bis-(4'-pyridylethynyl)arenes were synthesized and investigated for their photophysical and electrochemical properties, as well as their interactions with DNA/RNA. These compounds showed potential as therapeutic and diagnostic agents due to their interactions with DNA/RNA.
CHEMISTRY-A EUROPEAN JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Zoe Jelic Matosevic, Katarina Radman, Jolene Loubser, Ivo Crnolatac, Ivo Piantanida, Ignacy Cukrowski, Ivana Lescic Asler, Branimir Bertosa
Summary: The transcription factor MntR regulates Mn2+ ion homeostasis by binding to Mn2+ ions and enhancing its DNA-binding affinity. The binding of Mn2+ ions reduces the structural dynamics of MntR protein and shifts the conformation towards DNA binding. Additionally, Mn2+ binding also changes the helical structure and stability of MntR protein.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Pharmacology & Pharmacy
Marta Koscak, Isabela Pehar, Ksenija Bozinovic, Goutam Kumar Kole, Sandra Sobocanec, Iva I. Podgorski, Marija Pinteric, Klaus Muller-Buschbaum, Dragomira Majhen, Ivo Piantanida, Todd B. Marder
Summary: Certain methylated pyrene cations exhibit a high affinity for DNA and can be used as probes for DNA detection. Under light irradiation, these cations show increased toxicity against human cell lines and can cause damage to mitochondrial DNA.
Article
Biochemistry & Molecular Biology
Daniela Jaksic, Maja Segvic Klaric, Hrvoje Rimac, Robert Kerep, Ivo Piantanida
Summary: This study shows that sterigmatocystin (STC) can interact with cyclodextrins (CDs), with the highest affinity observed for sugammadex and gamma-CD. Molecular modelling and fluorescence spectroscopy revealed that STC inserts better into larger CDs. In addition, the study found that STC has a much lower affinity for human serum albumin (HSA) compared to sugammadex and gamma-CD, indicating that CDs can effectively displace STC from the STC-HSA complex.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Milena Mlakic, Ivona Cipor, Petra Kovacec, Goran Kragol, Ana Ratkovic, Tatjana Kovacevic, Rahela Zadravec, Valentina Milasinovic, Kresimir Molcanov, Ivo Piantanida, Irena Skoric
Summary: A new series of 4H-1,3-benzothiazine dyes were synthesized and characterized. Two synthetic pathways, Buchwald-Hartwig amination and electrochemical synthesis, were used to prepare benzothiazine salts. Electrochemical intramolecular dehydrogenative cyclization was employed to form 4H-1,3-benzothiazines. The binding of these compounds to polynucleotides was examined, and compounds 1 and 2 showed potential as DNA/RNA groove binders.
Article
Biochemistry & Molecular Biology
Lidija-Marija Tumir, Dijana Pavlovic Saftic, Ivo Crnolatac, Zeljka Ban, Matea Maslac, Stefanie Griesbeck, Todd B. Marder, Ivo Piantanida
Summary: A series of tetracationic bis-triarylborane dyes with different aromatic linkers showed high affinities towards ds-DNA and ds-RNA. The linker influenced the emissive properties of triarylborane cations and controlled the fluorimetric response of dyes. The different dye analogs exhibited selective or non-selective fluorescence responses and induced circular dichroism signals depending on the DNA/RNA sequences.
Article
Biotechnology & Applied Microbiology
Zeljka Ban, Antun Baris, Ivo Crnolatac, Sasa Kazazic, Sanja Skulj, Filippo Savini, Branimir Bertos, Ivan Barisic, Ivo Piantanida
Summary: The field of bio-nanoengineering has made significant progress in the past decade, allowing the generation of nanoscaled molecular machineries with arbitrary shapes. Functionalising complex molecules and nanostructures precisely is important to fully utilize novel methods like DNA origami technology. This study presents a method for selectively attaching oligonucleotides to glycosylated horseradish peroxidase protein (HRP) with high N-terminus selectivity and yield, while preserving enzymatic activity. The resulting protein-oligonucleotide conjugates were characterized using electrophoresis and mass spectrometry.
ENZYME AND MICROBIAL TECHNOLOGY
(2023)
Article
Pharmacology & Pharmacy
Marta Jurkovic, Marijana Radic Stojkovic, Ksenija Bozinovic, Davor Nestic, Dragomira Majhen, Estefania Delgado-Pinar, Mario Inclan, Enrique Garcia-Espana, Ivo Piantanida
Summary: This article introduces a novel polyamine compound that exhibits strong intramolecular interactions and has strong binding and antiproliferative activity, making it applicable in cancer and gene therapy.
Article
Chemistry, Medicinal
Marta Jurkovic, Matthias Ferger, Isabela Draskovic, Todd B. Marder, Ivo Piantanida
Summary: This study presents a novel triarylborane (TB)-dye that serves as both an NMe2 pi-donor and an inductive NMe2-alkyl cationic acceptor. The TB-dye is highly sensitive to a click reaction with an azide-substituted lysine side chain, resulting in a significant red shift of emission. Furthermore, the TB-dye exhibits high affinities towards both DNA and proteins, making it an ideal fluorimetric probe for detecting the progress of click reactions and sensing the binding site composition.
Article
Chemistry, Inorganic & Nuclear
Jorge Gonzalez-Garcia, Carolina Galiana, M. Auxiliadora Dea-Ayuela, Marijana Radic Stojkovic, Sonia Lopez-Molina, Cristina Galiana-Rosello, Salvador Blasco, Ivo Piantanida, Enrique Garcia-Espana
Summary: Metal complexes have become a focus of biomedical research due to their ability to explore unexplored chemical space and provide additional functionalities for diagnosis and therapy. This study evaluated the interaction between two polytopic polyaza ligands, their zinc complexes, and DNA/RNA using various spectroscopic assays. The results showed selective binding of zinc-PHENPOD complexes to GC-containing DNA and stabilization of AT-based DNA by PYPOD metal complexes. Additionally, the ligands and their trinuclear zinc complexes exhibited high antimicrobial activity. This research provides valuable insights into the nucle-arity of polytopic polyaza ligands in DNA/RNA binding and antimicrobial effects.
DALTON TRANSACTIONS
(2023)
Article
Chemistry, Inorganic & Nuclear
Silvio Jakopec, Lisa Gourdon-Grunewaldt, Ivona Cipor, Andrijana Mescic Macan, Berislav Peric, Ivo Piantanida, Kevin Cariou, Gilles Gasser, Srecko I. Kirin, Silvana Raic-Malic
Summary: Bioorganometallic complexes have shown promise for potential application in cancer treatment and diagnosis, as well as in bioimaging agents, with some acting as theranostic agents. A series of novel complexes with bidentate pyridyl-1,2,3-triazole and 2,2'-dipyridylamine ligands were prepared and characterized. The complexes exhibited interactions with biomacromolecules and showed significant antiproliferative activity on colon cancer cells.
DALTON TRANSACTIONS
(2023)
Article
Chemistry, Inorganic & Nuclear
Sasa Opacak, Margareta Pernar Kovac, Anamaria Brozovic, Ivo Piantanida, Srecko I. Kirin
Summary: Two novel pyrene triphenylphosphine ruthenium conjugates function as fluorescent turn-on beacons for serum albumin, exhibiting strong emission upon binding to BSA while being non-fluorescent in aqueous media. The compounds' selective cytotoxicity against tumour cells is enhanced under UV-light irradiation, suggesting potential application in two-photon excited photodynamic therapy.
DALTON TRANSACTIONS
(2023)
Meeting Abstract
Chemistry, Multidisciplinary
A. Visnjevac, N. Nyssen, I. Niksic-Franjic, I. Piantanida, B. Colasson, O. Reinaud
ACTA CRYSTALLOGRAPHICA A-FOUNDATION AND ADVANCES
(2022)
Article
Chemistry, Multidisciplinary
Ivona Krosl, Ena Otkovic, Ivana Niksic-Franjic, Benoit Colasson, Olivia Reinaud, Aleksandar Visnjevac, Ivo Piantanida
Summary: Research shows that calix[6]arene derivatives with a permanent positive charge can bind to DNA and RNA, providing more efficient stabilization for AT-DNA.
NEW JOURNAL OF CHEMISTRY
(2022)