Article
Polymer Science
A. Arzola-Rubio, C. Arzola-Alvarez, J. Camarillo-Cisneros, R. C. Anderson, O. Ruiz-Barrera, L. C. Hinojos-Gallardo, R. M. Cabral-Lares
Summary: A new amphiphilic copper compound Multiplex 1 has been designed to form nanoscale toroidal structures in water with a high affinity for DNA binding. The study showed that these compounds can successfully transfect genes into cells, suggesting potential applications in gene therapy.
JOURNAL OF INORGANIC AND ORGANOMETALLIC POLYMERS AND MATERIALS
(2021)
Article
Chemistry, Physical
Jong Dae Jang, Moongi Bae, Changwoo Do, Soo-Hyung Choi, Joona Bang, Young Soo Han, Tae-Hwan Kim
Summary: This study demonstrates the formation of a highly ordered superlattice of gold nanoparticles in a 2D confined geometry using hydrophobic interactions in a polymer vesicle layer. The use of block copolymers as templates allows for control over the degree of freedom of the gold nanoparticles, resulting in a centered rectangular structure formation.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2021)
Article
Polymer Science
Wanda Celentano, Stefania Ordanini, Riccardo Bruni, Luca Marocco, Pietro Medaglia, Andrea Rossi, Stefano Buzzaccaro, Francesco Cellesi
Summary: Well-defined amphiphilic PCL-PEG copolymers with complex molecular architectures were synthesized using ROP and ATRP. These copolymers were used to produce self-assembled nanoparticles for drug encapsulation, with the ability to tune self-assembly, particle size, and drug loading by designing specific macromolecular architectures.
EUROPEAN POLYMER JOURNAL
(2021)
Article
Polymer Science
Anh-Minh Nguyen-Huu, Ngoc Thuy Trang Le, Pham Nguyen Dong Yen, Yern Chee Ching, Dai Hai Nguyen
Summary: Quercetin has been extensively studied in drug delivery systems, and this study aims to use simplistic micelles to solve the problem of complicated systems. PEGylated-cholesterol micelles were prepared and characterized, and the results showed promising features of the nano-micelles as a drug delivery system.
JOURNAL OF APPLIED POLYMER SCIENCE
(2022)
Article
Chemistry, Multidisciplinary
Zhen Xu, Tianhui Shi, Fengye Mo, Wenqian Yu, Yu Shen, Qunying Jiang, Fuan Wang, Xiaoqing Liu
Summary: In this study, programmable DNA-protein superstructures were successfully assembled through biomimetic assembly, by combining programmable polymer DNA wire with alkaline protein protamine to form densely packed DNA nanospheres. These DNA-protein superstructures exhibited specifically high affinity and stability, enabling high cancer cell selectivity and reduced side effects.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Biochemistry & Molecular Biology
Yuan Liu, Jiaxin Wang, Lijun Sun, Bin Wang, Qiang Zhang, Xiaokang Zhang, Ben Cao
Summary: With the advancement of nanotechnology, DNA molecules have evolved from being solely carriers of genetic information to versatile materials with great potential in drug delivery and disease diagnosis. In this study, ladder-shaped drug carriers based on DNA nanoladders were developed. By incorporating nicks into each rung, the nanoladder structure was designed to counteract stability issues and provide drug loading sites. Experimental results showed that the designed drug-carrying function was feasible, demonstrating the potential application of this novel design approach in various fields such as bioimaging, biosensing, and targeted therapy.
Article
Chemistry, Physical
Vidhi Jain, Vashishtha B. Patel, Beena Singh, Dharmesh Varade
Summary: Microfluidics is a promising field with wide applications, providing fine control over particle size and speed in micro- and nanoscale self-assembled systems. It is a powerful tool for controlling physical and chemical properties of fluids and governing self-assembly processes, with potential impacts in various industries.
JOURNAL OF MOLECULAR LIQUIDS
(2022)
Article
Chemistry, Applied
Laura Gallego-Yerga, Cristina de la Torre, Francesco Sansone, Alessandro Casnati, Carmen Ortiz Mellet, Jose M. Garcia Fernandez, Valentin Cena
Summary: Cyclodextrin-calixarene giant amphiphiles are capable of encapsulating and delivering anticancer drugs to tumor cells with high efficiency, significantly enhancing therapeutic efficacy. The redox-responsive disulfide bridge modification in these amphiphiles allows for efficient cargo release in a tumor microenvironment, leading to potent inhibition of cancer cell proliferation.
CARBOHYDRATE POLYMERS
(2021)
Article
Engineering, Biomedical
Jinpeng Han, Yuchen Cui, Zi Gu, Dayong Yang
Summary: The study developed a nanocarrier system that can efficiently release drugs in cells while minimizing side effects, utilizing polyphenol-DNA nanocomplexes. Through programmable assembly and disassembly processes, this method achieves precise release of multiple genes and drugs in cells.
Article
Chemistry, Physical
Meiwen Cao, Zijin Zhang, Xiaoyang Zhang, Yu Wang, Jingjing Wu, Zhihong Liu, Li Sun, Dong Wang, Tongtao Yue, Yuchun Han, Yingxiong Wang, Yilin Wang, Ming Wang
Summary: Smart artificial viruses have been developed through co-assembly of peptides and DNA, achieving stimuli-responsiveness and efficient gene transfection in cancer cells. These viruses protect DNA from digestion and release genes efficiently through enzyme-triggered structure disassembly. They resemble natural viruses in terms of structure and function, making them promising gene vectors.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2022)
Article
Chemistry, Multidisciplinary
Zhen Zou, Libei He, Xiangxi Deng, Huangxiang Wang, Ziyun Huang, Qian Xue, Zhihe Qing, Yanli Lei, Ronghua Yang, Juewen Liu
Summary: The study reports the formation of Zn2+-driven RNA self-assembly forming spherical nanoparticles, while preserving the integrity and biological function of RNA, with excellent RNA-loading efficiency, pharmacokinetics, and bioavailability.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Chemistry, Multidisciplinary
Wantao Tang, Ting Tong, Hong Wang, Xuehe Lu, Changping Yang, Yushuai Wu, Yuang Wang, Jianbing Liu, Baoquan Ding
Summary: DNA nanostructures have been widely used in drug delivery systems. This study presents a DNA origami-based CRISPR/Cas9 gene editing system for efficient gene therapy in vivo. The designed DNA origami structure can recruit and load the sgRNA/Cas9 complex for targeted gene editing, and achieve effective drug delivery.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Physical
Alberto Scacchi, Maria Sammalkorpi, Tapio Ala-Nissila
Summary: This study demonstrates how intrinsic length scales and interspecies molecular interactions can be used to control self-assembly in soft matter systems using classical density functional theory. The results show that tuning intermolecular interactions and system composition can influence concentration gradients within the assemblies.
JOURNAL OF CHEMICAL PHYSICS
(2021)
Article
Engineering, Environmental
Shuzhen Yue, Zhenjie Qiao, Kaixin Yu, Xin Hai, Yuwei Li, Yuanfang Li, Weiling Song, Sai Bi
Summary: A novel DNA addition polymerization (DAP) reaction was proposed to guide the self-assembly of DNA towards the construction of size-controlled DNA nanospheres (DNSs), leading to the successful development of a versatile nano-drug delivery system. These multifunctional DNSs demonstrated good stability and biocompatibility in vitro and in vivo, showing excellent performance in cancer therapy.
CHEMICAL ENGINEERING JOURNAL
(2021)
Article
Chemistry, Multidisciplinary
Zhengqing Guo, Hui He, Yi Zhang, Jiaming Rao, Tao Yang, Ting Li, Lu Wang, Mengke Shi, Mengya Wang, Shihong Qiu, Xue Song, Hengte Ke, Huabing Chen
Summary: This study demonstrates the use of self-assembled micellar nanoarchitecture for potent phototherapy against triple-negative breast cancer, showing efficient suppression against primary, metastatic, and recurrent TNBC through tunable photoconversion and cytoplasmic translocation.
ADVANCED MATERIALS
(2021)
Article
Materials Science, Multidisciplinary
Nina Melnychuk, Pichandi Ashokkumar, Ilya O. Aparin, Andrey S. Klymchenko
Summary: Surface modification of nanoparticles plays a crucial role in providing desired functions for biological and biomedical applications. This study explored two approaches, pre- and postfunctionalization, for modifying dye-loaded nanoparticles, revealing different reaction rates based on the number of charged groups introduced. The findings provide insights into the surface reactivity of polymeric nanoparticles and offer guidance for designing effective functional nanoparticle probes for biosensing and bioimaging.
ACS APPLIED POLYMER MATERIALS
(2022)
Article
Chemistry, Analytical
Kyong Tkhe Fam, Remi Pelletier, Farah Bouhedda, Michael Ryckelynck, Mayeul Collot, Andrey S. Klymchenko
Summary: This study aims to develop a robust molecular tool for intracellular RNA imaging. By rational design, a fluorogenic self-quenched dimer was successfully developed, which showed good affinity and fluorescence response to its cognate aptamer both in solution and live cells, with a better limit of detection.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Sophie Michelis, Lydia Danglot, Romain Vauchelles, Andrey S. Klymchenko, Mayeul Collot
Summary: Tracking the pH variation of intracellular vesicles is crucial for assessing cell trafficking and metabolism. we designed a fluorogenic ratiometric pH probe, Mem-pH, which specifically targets the plasma membrane of cells and accumulates in intracellular vesicles through endocytosis. It allows for dynamic observation of vesicular acidification throughout the endocytic pathway and pH measurement through ratiometric imaging.
ANALYTICAL CHEMISTRY
(2022)
Review
Chemistry, Multidisciplinary
Andrey S. Klymchenko
Summary: Biomembranes, as lipid structures, play a crucial role in cellular processes. The development of chemical tools, such as fluorescent probes, for sensing and imaging biomembranes is a rapidly growing field. This Account focuses on advances in smart molecules for membrane targeting and sensing, with a particular emphasis on plasma membranes and organelles.
ACCOUNTS OF CHEMICAL RESEARCH
(2023)
Article
Chemistry, Multidisciplinary
Lazare Saladin, Ophelie Dal Pra, Andrey S. S. Klymchenko, Pascal Didier, Mayeul Collot
Summary: Dual-emissive photoconvertible fluorophores (DPCFs) are useful for tracking labeled cells in bioimaging. The directed photooxidation-induced conversion (DPIC) mechanism allows efficient DPCFs to be obtained by conjugating a coumarin to aromatic singlet-oxygen reactive moieties (ASORMs). Pyrrole is a suitable ASORM that provides a high hypsochromic shift and fast conversion. By modifying pyrrole, we can tune the photoconversion properties of the DPCFs, leading to an improved dual emissive converter, SCP-Boc, with high brightness and a 63% enhanced photoconversion yield. SCP-Boc was successfully used for sequential cell photoconversion using laser scanning confocal microscopy.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Chemistry, Physical
Germain A. Brou, Gildas K. Gbassi, Ievgen Shulov, Aidar Seralin, Andrey S. Klymchenko, Thierry F. Vandamme, Nicolas Anton
Summary: In this study, we investigated the characterization and tracking of chemical reactions of amphiphile compounds on model interfaces. By using a custom-made surfactant and interfacial crosslinking through click chemistry, we observed changes in monolayer properties and dilatational elasticity. Through bulk phase exchange and washing, we specifically measured the interfacial effects of the entities adsorbed and trapped at the interface.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2023)
Article
Nanoscience & Nanotechnology
Fei Liu, Nicolas Anton, Yosuke Niko, Andrey S. Klymchenko
Summary: Current biomedical applications of nanocarriers focus on controlled drug delivery, but this study proposes a different approach where toxic molecules are extracted from biological tissues using lipid nanoemulsion (NE) droplets. By designing a highly lipophilic amine (LipoAmine), cargo-aldehydes can be reacted and encapsulated inside lipid NEs through dynamic covalent chemistry. The formation of imine conjugates was validated and the NEs successfully extracted fluorescent dye and toxic aldehydes from cells, demonstrating the potential for developing detoxification platforms.
ACS APPLIED BIO MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Deep Sekhar Biswas, Paraskevi Gaki, Elisabete Cruz Da Silva, Antoine Combes, Andreas Reisch, Pascal Didier, Andrey S. Klymchenko
Summary: By studying the FRET phenomenon between fluorescent organic nanoparticles, it is found that the efficiency does not follow the Forster law and can achieve high efficiency at long distances. This opens up the possibility of using advanced optical nanomaterials for amplified FRET-based biosensing.
ADVANCED MATERIALS
(2023)
Article
Chemistry, Analytical
Reimi Pelletier, Dmytro I. Danylchuk, Hela Benaissa, Fanny Broch, Romain Vauchelles, Arnaud Gautier, Andrey S. Klymchenko
Summary: Genetically encoded protein labels combined with polarity-sensitive fluorescent probes provide information about the nanoscale environments of proteins within subcellular compartments. A solvatochromic HaloTag probe, NR12-Halo, specifically labels a variety of proteins localized in different cell compartments and distinguishes proteins within lipid membranes. This probe also reveals changes in protein environments during their life cycle, heterogeneity in membrane protein polarity, and the effects of mechanical stress and fatty acid diet on protein nanoenvironments. The solvatochromic HaloTag probe is a promising tool for studying protein interactions and nanoscale environments within subcellular structures.
ANALYTICAL CHEMISTRY
(2023)
Editorial Material
Chemistry, Multidisciplinary
L. Saladin, O. Dal Pra, A. S. S. Klymchenko, P. Didier, M. Collot
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Chemistry, Inorganic & Nuclear
Yuriy Boiko, Konstantin Belikov, Ekaterina Bryleva, Zinaida Bunina, Victoria Varchenko, Alexander B. Rozhenko, Andriy Drapailo, Roman Rodik, Alexander Golub, Alexander Katz, Vitaly Kalchenko
Summary: This article describes a one-pot sequential reaction of silica gel with a tetrachlorosilane/triethylamine mixture and then with a conical upper rim tetraphosphorylated tetrahydroxy(thia)calixarenes, resulting in porous organo-inorganic sorbents modified by spatially ordered phosphonate, phosphinate, or phosphine oxide groups capable of cooperative binding of metal cations. The grafting process improves the efficacy of europium(III) sorption by the silica gels. DFT calculations reveal that the calixarene-Eu3+ complexes adopt a flattened cone conformation with coordination of the Eu3+ cation to three of the four P = O oxygen atoms and one of the aromatic systems, while the inclusion of three water molecules completely excludes cation-pi interactions.
PHOSPHORUS SULFUR AND SILICON AND THE RELATED ELEMENTS
(2023)
Article
Chemistry, Physical
Tesla Yudhistira, Elisabete Cruz Da Silva, Antoine Combes, Maxime Lehmann, Andreas Reisch, Andrey S. S. Klymchenko
Summary: This study reports a method of antibody labeling using biotinylated zwitterionic dye-loaded polymeric nanoparticles. The prepared biotinylated nanoparticles are small (14 nm) and bright, capable of carrying a large amount of cationic rhodamine dye. The method significantly improves fluorescence imaging of target epidermal growth factor receptors and becomes a useful tool in the high-sensitivity detection of disease biomarkers.
Article
Materials Science, Multidisciplinary
Deep Sekhar Biswas, Nina Melnychuk, Caterina Severi, Pascal Didier, Andrey S. Klymchenko
Summary: Light-harvesting is a fundamental process in nature that drives the development of artificial systems for various applications. Researchers have discovered that using blank hydrophobic salts can suppress dye aggregation within nanoparticles, improving their fluorescence properties and enabling efficient energy transfer and antenna effects.
ADVANCED OPTICAL MATERIALS
(2023)
Article
Materials Science, Multidisciplinary
Lucie Haye, Nour Fayad, Richard C. Knighton, Antoine Combes, Olivier Jeannin, Andrey Klymchenko, Thibault Gallavardin, Niko Hildebrandt, Loic J. Charbonniere, Andreas Reisch
Summary: This study investigates the physicochemical and photophysical properties of Re diimine complexes and Cy5 dyes coencapsulated in polymer nanoparticles (NPs), and their compatibility for bioimaging. The results show that encapsulation significantly enhances the photoluminescence brightness and lifetimes of Re complexes, while coencapsulation of Cy5 leads to bright near-infrared emission upon Re complex excitation. Furthermore, the Re-Cy5 NPs exhibit high-contrast photoluminescence imaging in mammalian cells.
ADVANCED MATERIALS TECHNOLOGIES
(2023)
Review
Chemistry, Multidisciplinary
Anila Hoskere Ashoka, Ilya O. Aparin, Andreas Reisch, Andrey S. Klymchenko
Summary: Brightness is crucial for (bio)molecular detection and optical bioimaging. This tutorial review provides definitions of brightness and describes the major approaches to its analysis. It also discusses the current chemical methods to combat Aggregation-Caused Quenching and compares the brightness and other properties of different fluorescent organic nanoparticles. The importance of brightness in biological applications is also analyzed.
CHEMICAL SOCIETY REVIEWS
(2023)