期刊
CHEMISTRY-A EUROPEAN JOURNAL
卷 16, 期 2, 页码 556-561出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.200901168
关键词
acridine orange; carbon nanotubes; endocytosis; lysosomes
资金
- National Science Fund for Distinguished Young Scholars [50925310]
- National Science Foundation of China [20874059, 20904030]
- Chinese National Programs for Fundamental Research and Development [2009CB930400]
- High Technology Research and Development program of China [2009AA03Z329]
- Science and Technology Commission of the Shanghai Municipal Government [08JC1412300]
- Shanghai Leading Academic [B202]
Fluorescent single-wall carbon nanotubes (SWCNTs) were prepared by mixing Cut SWCNTs with acridine orange (AO). The optical absorbance and fluorescence characteristics of AO-SWCNT conjugates display interesting pH-dependent properties. Fluorescence microscopy in combination with transmission electron microscopy proves that AO-SWCNTs can enter HeLa cells and are located inside lysosomes. The endocytosis-inhibiting tests show that the clathrin-mediated endocytosis is a key step in the internalization process. The internalized AO-SWCNTs remain inside lysosomes for more than a week and have little effect on cell proliferation. These findings may be useful in understanding the SWCNT-based intracellular drug, delivery mechanism and help to develop new intracellular drug transporters.
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