期刊
CHEMISTRY-A EUROPEAN JOURNAL
卷 17, 期 5, 页码 1642-1648出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.201001856
关键词
aptamers; biosensors; chemiluminescence; gold; nanoparticles
资金
- National Natural Science Foundation of China [21075080]
- Chinese Ministry of Education [109150]
- Fundamental Research Funds for the Central Universities [GK20091004]
We report a label-free, aptamer-based chemiluminescent biosensor. The biosensor relies upon the catalytic activity of unmodified gold nanoparticles (AuNPs) on the luminol-H2O2 chemiluminescence (CL) reaction, and the interaction of unmodified AuNPs with the aptamer. The unmodified AuNPs can effectively differentiate unstructured and folded aptamer. The binding of the aptamer with the target can induce the AuNP aggregation in the presence of 0.5m NaCl, and after aggregation the catalytic activity of the AuNPs on the luminol H2O2 CL reaction is greatly enhanced. During the assay, no covalent functionalization of the AuNPs or aptamer is required. The detection limit of thrombin was estimated to be as low as 26 fM, and the sensitivity was more than 4 orders of magnitude better than that of known AuNP-based colorimetric methods for the detection of thrombin. This aptamer-based biosensor offers the advantages of being simple, cheap, rapid, and sensitive
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