期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 54, 期 20, 页码 5948-5951出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201500342
关键词
capture reagents; lipidation; mass spectrometry; post-translational modification; proteomics
资金
- Deutsche Forschungsgemeinschaft [Br 4387/1-1]
- European Union [PIEF-GA-2011-299740, PIEF-GA-2010-273868]
- Imperial College London Institute of Chemical Biology EPSRC Centre for Doctoral Training studentship [EP/F500416/1]
- EPSRC [EP/K039946/1, EP/J021199/1] Funding Source: UKRI
- MRC [MR/L000148/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [978583] Funding Source: researchfish
- Engineering and Physical Sciences Research Council [EP/J021199/1, EP/K039946/1] Funding Source: researchfish
- Medical Research Council [MR/L000148/1] Funding Source: researchfish
Novel multifunctional reagents were applied in combination with a lipid probe for affinity enrichment of myristoylated proteins and direct detection of lipid-modified tryptic peptides by mass spectrometry. This method enables high-confidence identification of the myristoylated proteome on an unprecedented scale in cell culture, and allowed the first quantitative analysis of dynamic changes in protein lipidation during vertebrate embryonic development.
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