期刊
CHEMISTRY-A EUROPEAN JOURNAL
卷 14, 期 34, 页码 10727-10734出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.200801503
关键词
electrocatalysis; electrochemistry; monolayers; proteins; self-assembly
资金
- NSFC [20631010, 20775034, 20535010]
- National Science Fund for Creative Research Groups [20521503, 20721002]
- National Basic Research Program of China [2007CB925102]
Phosphonic acid (-PO3H2) terminated self-assembled monolayers (SAMs) on a gold surface were used as a functional interface to immobilize hemoglobin (Hb). In situ surface-enhanced infrared absorption spectroscopy (SEIRAS) measurements show that Hb immobilization is a sluggish process due to formation of multilayer Hb structures on the PO3H2-terminated SAMs. as revealed by ellipsometry, atomic force microscopy (AFM). and cyclic voltammetry (CV). In the multilayered Hb film the innermost Hb molecules can directly exchange electrons With the electrode, whereas Hb beyond this layer communicates electronically With the electrode via protein-protein electron exchange. In ad clition, electrochemical measurements indicate that immobilization of Hb on the PO3H2-terminated SAMs is not driven by the electrostatic interaction, but likely by hydrogen-bonding interaction. The immobilized Hb molecules show excellent bioelectrocatalytic activity towards hydrogen peroxide, that is, the PO3H2-terminated SAMs are promising for construction of third-generation biosensors.
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