期刊
CHEMISTRY LETTERS
卷 40, 期 4, 页码 381-383出版社
CHEMICAL SOC JAPAN
DOI: 10.1246/cl.2011.381
关键词
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资金
- MEXT, Japan
- Grants-in-Aid for Scientific Research [22580093] Funding Source: KAKEN
A fed-batch process was used to produce cis,cis-muconic acid from catechol by recombinant Escherichia coli cells expressing the catA gene, which encodes the Pseudomonas putida mt-2 catechol I,2-dioxygenase responsible for catalyzing ortho-cleavage of catechol, as biocatalysts. We succeeded in producing 415 mM (59.0 g L-1) cis,cis-muconic acid in aqueous solution without generation of by-products in 12 h under the optimal conditions with successive addition of 10 mM catechol. The molar conversion yield based on the amount of consumed catechol was the theoretical value of 100% (mol mol(-1)).
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