Article
Biochemistry & Molecular Biology
Lei Liu, Shujuan Luan, Chunli Zhang, Rui Wang, Yanan Zhang, Mengying Zhang, Qianli Sheng, Guang Han, Tianshun Wang, Shiyong Song
Summary: By introducing dopamine groups onto the skeleton of nanogels, efficient loading and pH-controlled release of bortezomib were achieved, leading to enhanced bioavailability and therapeutic effect of the drug. The pH-sensitive borated bonds between bortezomib and catechol groups endowed the drug with pH-responsive release behavior. In vivo experiments demonstrated that loading bortezomib into nanogels significantly enhanced its anti-tumor effect.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Polymer Science
Ziyang Xue, Rongzhan Fu, Zhiguang Duan, Lei Chi, Chenhui Zhu, Daidi Fan
Summary: In this study, a pH-responsive nanogel was prepared as a potential nanocarrier for CK to enhance its antitumor effects against lung cancer. The nanogel efficiently encapsulated CK and showed high drug loading and encapsulation efficiency. In vitro and in vivo experiments demonstrated the superior inhibitory rates of CK-loaded nanogels against tumor volume compared to CK alone.
Article
Nanoscience & Nanotechnology
Shenglan Zhao, Yu Xia, Qian Lan, Quanxin Wu, Yun Liu, Xiaoya Feng
Summary: Researchers developed an infection microenvironment-responsive nanogel (STF) for the synergistic application of photothermal therapy (PTT) and chemodynamic therapy (CDT) in bacterial infection. The STF nanogel exhibited excellent photothermal effect and catalytic activity, and in vitro and in vivo antibacterial assays demonstrated its satisfactory bactericidal efficiency. The developed intelligent bactericidal nanogel has great potential to be used as an antibiotic alternative to avoid bacterial resistance in future clinical treatments.
ACS APPLIED NANO MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Gulcan Geyik, Enes Guncum, Nuran Isiklan
Summary: The study focuses on designing and developing pH-responsive alginate-based nanogels for controlled release of etoposide. The ALG-g-PHPMA nanogels show a good drug encapsulation efficiency and pH-dependent release profile. These nanogels can be used as a pH-dependent nanoplatform for delivering anticancer drugs.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Chenlong Li, Huimin Zhou, Jingjiang Sun, Deshuo Wang, Jun Chang, Qingfu Wang, Yanan Li, Wei Zhao
Summary: F-19 MRI-assisted drug delivery allows for real-time monitoring and tracking of drug transportation. Amphiphilic block copolymers containing hydrophilic polyethylene glycol and F-19-containing hydrophobic segments were synthesized, with the photolysis behavior of the copolymers controlled using o-nitrobenzyl oxygen. Increasing the hydrophobic chain length enhanced drug loading capacity and photoresponsivity, while reducing chain mobility and FMRI-F-19 signal. Nanoparticles with a PTFEA polymerization degree of 10 exhibited detectable FMRI-F-19 signals and sufficient drug loading capacity (loading efficiency = 10%, cumulative release = 49%), providing a promising smart theranostic platform for F-19 MRI.
Article
Biophysics
Hong Yu Yang, Moon-Sun Jang, Yi Li, Jia Meng Du, Changling Liu, Jung Hee Lee, Yan Fu
Summary: In this study, a chlorine e6-functionalized and pH-responsive dynamically cross-linked nanogel was developed for co-delivery of photosensitizers and protein drugs to enhance the efficiency of synergistic cancer therapy. The nanogel demonstrated excellent systemic stability under normal physiological conditions and could be further cationized to enhance cell uptake in acidic extracellular environment. Upon internalization, the nanogel could release the photosensitizers and protein drugs, leading to the generation of cytotoxic singlet oxygen and improved photodynamic therapy of cancer.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Article
Chemistry, Physical
Mingning Zhu, Dongdong Lu, Amir H. Milani, Najet Mahmoudi, Stephen M. King, Brian R. Saunders
Summary: The study utilized small-angle neutron scattering (SANS) to investigate the structural changes of nanogels (NGs) in dispersion and gels at different pH conditions. Results showed that dispersed NGs have a core-shell structure, and NGs in PAAm gel exhibit consistent changes with increasing pH, as observed for dispersed NGs.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2022)
Article
Engineering, Biomedical
Minghua Li, Junjun Tang, Chao Lin, Aijun Shen, Xiaolong Ma, Jiaqi Wu, Xiaolong Gao, Peijun Wang
Summary: A smart responsive fluorescence-MR dual-modal nanoprobe, QM(GP)-MZF(CP), is reported which can be targeted and cleaved by granzyme B and Caspase-3. The probe uses quinoline-malononitrile (QM) and Mn-Zn ferrite magnetic nanoparticles (MZF-MNPs) as imaging molecules, linked with substrate peptides specific to the markers. Through fluorescence imaging and T2-weighted imaging, the nanoprobe can determine the status of T cells and tumor cell apoptosis after immunotherapy, evaluating the effectiveness of the treatment.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Pharmacology & Pharmacy
G. R. Nirmal, Chia-Chih Liao, Zih-Chan Lin, Abdullah Alshetaili, Erica Hwang, Shih-Chun Yang, Jia-You Fang
Summary: This study established an alkyl radical-based therapy by loading AIPH into chitosan nanogels to treat psoriatic hyperplasia. The nanogels exhibited pH-responsiveness and allowed fast release of alkyl radicals in the target site. The results showed that the nanogels successfully delivered alkyl radicals into the epidermis, reducing hyperplasia without causing irritation.
Article
Biochemistry & Molecular Biology
Ghada A. Mahmoud, Hussein E. Ali, Rasha R. Radwan
Summary: A polymeric pH-sensitive nanocarrier was synthesized to deliver pyrogallol, and its anti-tumor activity against colon cancer in rats was investigated. The results showed that pyrogallol-loaded polymeric nanogel significantly reduced tumor incidence and volume. It activated apoptotic pathway and attenuated cell cycle progression. It also exhibited anti-proliferative activity and reduced pro-inflammatory cytokines. The histopathological examination confirmed the results. This formulation could be considered as a promising agent in the treatment of colon cancer.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2022)
Article
Biotechnology & Applied Microbiology
Enping Chen, Ting Wang, Junmei Zhang, Xiang Zhou, Yafan Niu, Fu Liu, Yinan Zhong, Dechun Huang, Wei Chen
Summary: The study developed a mitochondria-directed pH-sensitive PVA nanogel that combined hexokinase inhibitor and chemotherapeutic drug, enhancing drug loading capacity, promoting drug release, and achieving mitochondrial targeting within cells, leading to significant anti-tumor effects.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Alvja Mali, Margot Verbeelen, Paul B. White, Alexander H. J. Staal, N. Koen van Riessen, Cyril Cadiou, Francoise Chuburu, Olga Koshkina, Mangala Srinivas
Summary: F-19 magnetic resonance imaging (F-19 MRI) is a promising technique for quantitative imaging in novel therapies. By using biodegradable polymeric nanoparticles with a multicore structure, the F-19 signal can be modulated and the nanoparticles can be sensitive to different pH environments.
Article
Chemistry, Physical
Adrian Ges Naranjo, Herlys Viltres Cobas, Nishesh Kumar Gupta, Katy Rodriguez Lopez, Aldo Martinez Pena, Daniel Sacasas, Ruben Alvarez Brito
Summary: Nanogels offer a promising approach for enhancing cancer therapy by increasing the concentration of anti-tumor agents at tumor sites and prolonging their effects on cancer cells without significantly impacting normal tissues.
JOURNAL OF MOLECULAR LIQUIDS
(2022)
Article
Biochemistry & Molecular Biology
Kristyna Kolouchova, Ondrej Groborz, Zulfiya Cernochova, Aneta Skarkova, Jan Brabek, Daniel Rosel, Pavel Svec, Zenon Starcuk, Miroslav Slouf, Martin Hruby
Summary: In this study, polymer tracers for F-19 MRI were modified by introducing a redox-responsive monomer, allowing for changes in physicochemical properties in an oxidative environment. These ROS-responsive polymers have potential applications in theranostic purposes, especially in terms of drug delivery systems sensitive to oxidative environments in tumors or inflamed tissues.
Article
Chemistry, Multidisciplinary
Shiya Chen, Mingjian Chen, Jinfeng Yang, Xianqing Zeng, Yibo Zhou, Sheng Yang, Ronghua Yang, Quan Yuan, Jing Zheng
Summary: Hypoxia and acidic pH are chosen as dual stimuli to fabricate a dual lock-and-key fluorescent nanoprobe for highly specific and precise imaging of tumor cells. This nanoprobe successfully applies in a tumor-bearing mice model, indicating the potential for early diagnosis and assessment of cancer treatment.
Article
Biochemistry & Molecular Biology
Anton V. Lopukhov, Zigang Yang, Matthew J. Haney, Tatiana K. Bronich, Marina Sokolsky-Papkov, Elena V. Batrakova, Natalia L. Klyachko, Alexander V. Kabanov
Summary: The use of mannose-decorated polyplexes has shown significantly enhanced gene transfection efficiency in macrophages, especially in the presence of Man-PEG-b-pAsp(DET). While cross-linked polyplexes reduced toxicity, the impact on transfection enhancement was moderate. This suggests that Man-PEG-b-pAsp(DET)/pDNA polyplex may be a potential vector for immune cells-based gene therapy.
MACROMOLECULAR BIOSCIENCE
(2021)
Correction
Chemistry, Multidisciplinary
Xiang Yi, Dongfen Yuan, Susan A. Farr, William A. Banks, Chi-Duen Poon, Alexander V. Kabanov
JOURNAL OF CONTROLLED RELEASE
(2022)
Article
Chemistry, Multidisciplinary
Chaemin Lim, Jacob D. Ramsey, Duhyeong Hwang, Susana C. M. Teixeira, Chi-Duen Poon, Joshua D. Strauss, Elias P. Rosen, Marina Sokolsky-Papkov, Alexander Kabanov
Summary: The study found that the morphology of polymeric micelles significantly affects pharmacological efficacy, with spherical micelles accumulating rapidly in tumor tissue while retaining large amounts of drug, and worm-like micelles accumulating more slowly and only upon releasing significant amounts of drug.
Article
Multidisciplinary Sciences
Chaemin Lim, Taylor Dismuke, Daniel Malawsky, Jacob D. Ramsey, Duhyeong Hwang, Virginia L. Godfrey, Alexander Kabanov, Timothy R. Gershon, Marina Sokolsky-Papkov
Summary: The combination of CDK4/6 inhibitor palbociclib nanoparticle formulation (POx-Palbo) with mTORC1 inhibitor sapanisertib has shown significant therapeutic effects on SHH medulloblastoma, prolonging mouse survival by suppressing mTORC1 signaling and inhibiting cell proliferation.
Meeting Abstract
Biochemistry & Molecular Biology
Carla Freire, Huv Pho, Jacob D. Ramsev, Yuling Zhao, Lenise J. Kim, Slava Berger, Frederick Anokye-Danso, Luiz U. Sennes, Rexford S. Ahima, Elena V. Batrakova, Alexander V. Kabanov, Vsevolod Y. Polotsky
Article
Biochemistry & Molecular Biology
Jacob D. Ramsey, Ian E. Stewart, Emily A. Madden, Chaemin Lim, Duhyeong Hwang, Mark T. Heise, Anthony J. Hickey, Alexander Kabanov
Summary: The novel coronavirus has caused a global pandemic with no approved treatment. Poly(2-oxazoline) block copolymers have been found to stabilize drug nanocrystal suspensions and are suitable for aerosol delivery while maintaining antiviral efficacy.
MACROMOLECULAR BIOSCIENCE
(2022)
Correction
Multidisciplinary Sciences
Alyssa M. Master, Philise N. Williams, Nikorn Pothayee, Nipon Pothayee, Rui Zhang, Hemant M. Vishwasrao, Yuri I. Golovin, Judy S. Riffle, Marina Sokolsky, Alexander V. Kabanov
SCIENTIFIC REPORTS
(2022)
Review
Pharmacology & Pharmacy
Ana Figueiras, Catia Domingues, Ivana Jarak, Ana Isabel Santos, Ana Parra, Alberto Pais, Carmen Alvarez-Lorenzo, Angel Concheiro, Alexander Kabanov, Horacio Cabral, Francisco Veiga
Summary: Nanomedicine, specifically the study of polymeric micelles (PMs), has emerged as a significant area of medicine in the last decade. PMs are colloidal dispersions of amphiphilic compounds that assemble into a core-shell structure, with a hydrophobic core and a hydrophilic shell. They improve the solubility and stability of drugs and can be absorbed and transported to the therapeutic target. PMs have high load capacity and are synthesized using a reproducible and cost-effective method. pH and temperature are commonly studied stimuli for enhanced drug release.
Article
Pharmacology & Pharmacy
James M. Fay, Chaemin Lim, Anna Finkelstein, Elena Batrakova, Alexander Kabanov
Summary: The study introduced two novel formulations replacing PEG in therapeutic polyion complexes, demonstrating that encapsulation does not inhibit receptor kinase access, enhancing the physiological benefits of BDNF.
Article
Chemistry, Multidisciplinary
Maxim M. Veselov, Igor Uporov, Maria Efremova, Irina M. Le-Deygen, Andrey N. Prusov, Igor Shchetinin, Alexander G. Savchenko, Yuri Golovin, Alexander Kabanov, Natalia L. Klyachko
Summary: The conjugation of enzymes to magnetic nanoparticles can lead to changes in their catalytic activity under nonheating low-frequency magnetic fields. In this study, alpha-chymotrypsin (CT) conjugated to gold-coated iron oxide nanoparticles was investigated. The results showed that the stretching force applied to the CT globule by the amino or carboxylic groups attached to the nanoparticles caused shrinkage of the substrate-binding site, but had little effect on the catalytic triad. The magnetic field altered the Michaelis-Menten constant, but not the apparent catalytic constant. Additionally, the secondary structure of CT was affected, with a loss of alpha-helices and an increase in random structures. However, both the catalytic activity and the protein structure of the conjugates were restored after 3 hours of field exposure. These remotely actuated systems have potential applications in advanced manufacturing, nanomedicine, and other areas.
Article
Nanoscience & Nanotechnology
Juan Beltran-Huarac, Dina N. Yamaleyeva, Gianpietro Dotti, Shawn Hingtgen, Marina Sokolsky-Papkov, Alexander Kabanov
Summary: This study developed a noninvasive therapeutic approach using magneto-mechanical actuation to regulate the expression of the tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) protein. By using magnetic nanoparticles as the control medium, the expression of therapeutic proteins in cells can be remotely regulated at low magnetic field strengths, enabling selective killing of cancer cells.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Biochemistry & Molecular Biology
Dina N. Yamaleyeva, Naoki Makita, Duhyeong Hwang, Matthew J. Haney, Rainer Jordan, Alexander V. Kabanov
Summary: The present study aims to expand the application of cationic poly(2-oxazoline) (POx) copolymers as a PEG-free platform for gene delivery to immune cells. By varying the composition of hydrophilic, cationic, and hydrophobic blocks in the copolymers, different structures have been developed and evaluated for their transfection efficiency in macrophages. The results indicate that the DET-containing diblock copolymer, with no targeting ligand, exhibits the highest transfection efficiency among the studied structures.
MACROMOLECULAR BIOSCIENCE
(2023)
Article
Materials Science, Biomaterials
Mohamed F. Attia, Edikan A. Ogunnaike, Megan Pitz, Nancy M. Elbaz, Dillip K. Panda, Angela Alexander-Bryant, Sourav Saha, Daniel C. Whitehead, Alexander Kabanov
Summary: Drug delivery systems based on amphiphilic supramolecular macrocycles have gained significant attention due to their ability to formulate nanoparticles. In this study, we developed amphiphilic macrocycle nanoparticles capable of delivering both hydrophobic and hydrophilic fluorescent dyes as well as the CDK4/6 inhibitor Palbociclib. These nanoparticles exhibited high stability and low toxicity, and showed selective targeting towards melanoma cells.
BIOMATERIALS SCIENCE
(2023)
Letter
Biochemistry & Molecular Biology
Carla Freire, Jacob D. Ramsey, Huy Pho, Ryo Kojima, Yuling Zhao, Lenise Kim, Frederick Anokye-Danso, Slava Berger, Rexford S. Ahima, Elena V. Batrakova, Alexander V. Kabanov, Vsevolod Y. Polotsky
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Maxim M. Veselov, Igor V. Uporov, Maria V. Efremova, Irina M. Le-Deygen, Andrey N. Prusov, Igor V. Shchetinin, Alexander G. Savchenko, Yuri I. Golovin, Alexander V. Kabanov, Natalia L. Klyachko
Summary: This study investigates the changes in enzymatic activity and protein structure of enzymes conjugated to gold-coated iron oxide magnetic nanoparticles. The results show that a low-frequency magnetic field can alter the enzyme kinetics and secondary structure. However, the changes can be restored after 3 hours.
